Journal: Science Advances

Article Title: Whole-body in vivo MPI cytometry reveals injection route-, dose-, cell size–, and disease-dependent differences in organ distribution

doi: 10.1126/sciadv.aec4482

Figure Lengend Snippet: ( A ) Macro- and microscopic visualization of ferucarbotran-labeled hMSCs before injection. ( B ) In vivo MPI/CT images of labeled hMSCs at 30 min and 1 day after intravenous or intra-arterial injection. S.I., signal intensity. ( C ) Quantitative analysis of cell accumulation 30 min post–intra-arterial injection. Two fiducials (F1 = 10,000 and F2 = 20,000 labeled cells) were used for calibration ( y = 0.13 x − 94.92; R 2 = 0.98). Data in each figure panel represent three independent experiments. B, Lu, Li, and F stand for the brain, lungs, liver, and fiducials, respectively.

Article Snippet: To this end, hMSCs were incubated for 24 hours with a pre-prepared labeling medium consisting of MSC basal medium supplemented with ferucarbotran (25 μg Fe/ml, Meito Sangyo Co.) and poly- l -lysine (PLL; Sigma-Aldrich, P-1524, 3 μg/ml).

Techniques: Labeling, Injection, In Vivo

Journal: Science Advances

Article Title: Whole-body in vivo MPI cytometry reveals injection route-, dose-, cell size–, and disease-dependent differences in organ distribution

doi: 10.1126/sciadv.aec4482

Figure Lengend Snippet: Representative ex vivo MPI and histological analysis of ferucarbotran-labeled hMSCs ( A ) and Synomag-D70–labeled hNPCs ( B ) 6 hours after intra-arterial injection. Colocalization of positive PB and anti-HuNa staining confirms the presence of SPIO-labeled hMSCs in the brain.

Article Snippet: To this end, hMSCs were incubated for 24 hours with a pre-prepared labeling medium consisting of MSC basal medium supplemented with ferucarbotran (25 μg Fe/ml, Meito Sangyo Co.) and poly- l -lysine (PLL; Sigma-Aldrich, P-1524, 3 μg/ml).

Techniques: Ex Vivo, Labeling, Injection, Staining

Journal: Nanoscale Advances

Article Title: Magnetic particle spectroscopy for Eu-VSOP quantification in intestinal inflammation: distinguishing nanoparticle signals from dietary contamination

doi: 10.1039/d5na00452g

Figure Lengend Snippet: MPS measurements of different MNP types (VSOP, Endorem®, Resovist®, MCP) reveal distinct signal characteristics in both the amplitude ( A n ) (A) and phase spectrum ( ϕ n ) (B). A two-dimensional plot of the phase ( ϕ 3 ) and amplitude ratio ( A 5 / A 3 ) further enables clear differentiation between MNP types (C). While environmental factors such as cellular uptake, pH variations, and ionic strength influence the magnetic response, the different MNP types remain clearly distinguishable by MPS. The dashed circles indicate the regions where the signals of the different MNP types are located and serve as visual guides only.

Article Snippet: Furthermore, we used two commercially available MNP systems, namely Ferucarbotran (marketed under the name Resovist® by Bayer Schering Pharma, GER) as well as Ferumoxides (marketed under the name Endorem® by Guerbet, FRA).

Techniques: