Journal: Scientific Reports

Article Title: Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters

doi: 10.1038/s41598-020-70291-9

Figure Lengend Snippet: Prussian blue (blue)/eosin (pink) staining of the unlabeled and iron-oxide-labeled UC-MSCs. The cells were cultured with 50 or 100 µg/mL of SPIO or USPIO particles. Scale bars, 50 µm. UCMSCs umbilical cord-derived mesenchymal stem cells, SPIO superparamagnetic iron oxide, USPIO ultrasmall superparamagnetic iron oxide.

Article Snippet: The UC-MSCs were labeled for 24 h with a SPIO (Resovist; Fujifilm Toyama Chemical Co., Ltd., Tokyo, Japan) or USPIO (MIRB; BioPAL, Worcester, MA, USA), at final concentrations of 50 or 100 μg/mL, then, washed three times with phosphate-buffered saline (PBS) (Nakalai Tesque Inc., Kyoto, Japan).

Techniques: Staining, Labeling, Cell Culture, Derivative Assay

Journal: Scientific Reports

Article Title: Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters

doi: 10.1038/s41598-020-70291-9

Figure Lengend Snippet: The effects of labelling on UCMSCs ( A ) Cell viability as determined by the Trypan blue exclusion assay. Viability is calculated as follows: percent viable cells = 100 × (number of viable cells per mL of aliquot)/(total number of cells per mL of aliquot). No significant impairment is observed. ( B ) Cell proliferation, as evaluated by the MTT assay (MTT: 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide). Proliferation as a percent is calculated as follows: 100 × (optical density of “treated” well − blank)/(optical density of “control” well − blank) (i.e., the optical density measurements are scaled to those of the untreated control cells). A significant impairment is observed at 100 μg/mL of USPIO. * P < 0.05 compared with control. UCMSCs umbilical cord-derived mesenchymal stem cells, SPIO superparamagnetic iron oxide, USPIO ultrasmall superparamagnetic iron oxide.

Article Snippet: The UC-MSCs were labeled for 24 h with a SPIO (Resovist; Fujifilm Toyama Chemical Co., Ltd., Tokyo, Japan) or USPIO (MIRB; BioPAL, Worcester, MA, USA), at final concentrations of 50 or 100 μg/mL, then, washed three times with phosphate-buffered saline (PBS) (Nakalai Tesque Inc., Kyoto, Japan).

Techniques: Trypan Blue Exclusion Assay, MTT Assay, Control, Derivative Assay

Journal: Scientific Reports

Article Title: Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters

doi: 10.1038/s41598-020-70291-9

Figure Lengend Snippet: The effect of the labelling protocol on UCMSC differentiation is presented. ( A ) Adipogenic differentiation is detected by Oil red O staining; all normal. ( B ) Osteogenic differentiation is detected by Alizarin red S staining; all normal. ( C ) Chondrogenic differentiation is detected by Alcian blue staining; a small impairment is observed at 100 μg/mL of SPIO. Oil red O and Alizarin red S: scale bar, 100 µm; Alcian blue: scale bars, 200 µm. UCMSCs umbilical cord-derived mesenchymal stem cells, SPIO superparamagnetic iron oxide, USPIO ultrasmall superparamagnetic iron oxide.

Article Snippet: The UC-MSCs were labeled for 24 h with a SPIO (Resovist; Fujifilm Toyama Chemical Co., Ltd., Tokyo, Japan) or USPIO (MIRB; BioPAL, Worcester, MA, USA), at final concentrations of 50 or 100 μg/mL, then, washed three times with phosphate-buffered saline (PBS) (Nakalai Tesque Inc., Kyoto, Japan).

Techniques: Staining, Derivative Assay

Journal: Scientific Reports

Article Title: Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters

doi: 10.1038/s41598-020-70291-9

Figure Lengend Snippet: T 2 values (contrast) in MRI of phantoms containing different concentrations of unlabeled and labeled UC-MSCs. USPIO shows a dose-dependent effect. * P < 0.05, compared with control group; # P < 0.05, compared with concentration = 0.25 × 10 6 cells/mL in the USPIO series. UCMSCs umbilical cord-derived mesenchymal stem cells, SPIO superparamagnetic iron oxide, USPIO ultrasmall superparamagnetic iron oxide, MRI magnetic resonance imaging.

Article Snippet: The UC-MSCs were labeled for 24 h with a SPIO (Resovist; Fujifilm Toyama Chemical Co., Ltd., Tokyo, Japan) or USPIO (MIRB; BioPAL, Worcester, MA, USA), at final concentrations of 50 or 100 μg/mL, then, washed three times with phosphate-buffered saline (PBS) (Nakalai Tesque Inc., Kyoto, Japan).

Techniques: Labeling, Control, Concentration Assay, Derivative Assay, Magnetic Resonance Imaging

Journal: Scientific Reports

Article Title: Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters

doi: 10.1038/s41598-020-70291-9

Figure Lengend Snippet: In vivo results: ( A ) T 2 -weighted MRI of the control (upper, n = 1), SPIO (middle, n = 4), and USPIO (lower, n = 4) animal groups at 0, 7, and 14 days after injection of UC-MSCs. ( B ) Micrographs of the low-signal-intensity regions observed on MRI at day 14. SPIO and USPIO deposits are demonstrated in the left hemisphere by Prussian-blue staining. Scale bars, 500 μm. UCMSCs umbilical cord-derived mesenchymal stem cells, SPIO superparamagnetic iron oxide, USPIO ultrasmall superparamagnetic iron oxide, MRI magnetic resonance imaging.

Article Snippet: The UC-MSCs were labeled for 24 h with a SPIO (Resovist; Fujifilm Toyama Chemical Co., Ltd., Tokyo, Japan) or USPIO (MIRB; BioPAL, Worcester, MA, USA), at final concentrations of 50 or 100 μg/mL, then, washed three times with phosphate-buffered saline (PBS) (Nakalai Tesque Inc., Kyoto, Japan).

Techniques: In Vivo, Control, Injection, Staining, Derivative Assay, Magnetic Resonance Imaging