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reg3a elisawas performedwith sandwich elisa kits  (R&D Systems)


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    R&D Systems reg3a elisawas performedwith sandwich elisa kits
    Reg3a Elisawas Performedwith Sandwich Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reg3a+elisa+kit/Human+Reg3A+DuoSet+ELISA/pm38589392-214-0-8
    Average 92 stars, based on 13 article reviews
    reg3a elisawas performedwith sandwich elisa kits - by Bioz Stars, 2026-11
    92/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: WJMSC‐derived small extracellular vesicle enhance T cell suppression through PD‐L1
    Article Snippet: Samples from blood plasma, cell culture supernatant and purified WJMSC sEVs were added to 96‐wll plate to detect the human PD‐L1 using a Human/Cynomolgus Monkey B7‐H1 Elisa kit according to the manufacturer's instructions (R&D systems, USA). .. For detection of IFN‐γ, ST2 and REG3 alpha (REG3A), plates were prepared using Duoset human IFN‐γ, ST2 and REG3A ELISA kit according to the manufacturers’ instructions (R&D systems, USA). .. Coated plates were added by the diluted plasma samples from aGvHD patients and detected by ELISA detection antibodies (R&D systems, USA).



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    Overview plasma markers analyzed in the present study
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    Decreased plasma ST2 and <t>REG3A</t> levels in aGvHD patients with WJMSC infusion. (a,b) ELISA of ST2 (a) and REG3A (b) in the plasma samples from aGvHD patients with the WJMSC infusion. Left, combined analysis; Middle, low‐dose (1.2 × 10 6 /kg WJMSCs); Right, high‐dose (10 × 10 6 /kg WJMSCs). Two cell infusion time points were indicated by red arrows (a,b). Data are mean ± s.e.m. and analysed by unpaired one‐tailed Student's t‐test (a,b). * P < 0.05, ** P < 0.01, *** P < 0.005
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    Plasma analysis of inflammatory cytokines IL-2. (A) IL-6 (B) and IL-8 (C) in patient PB. Analysis of GVHD biomarkers ST-2 (D) , osteopontin (E) and follistatin (F) in patient PB. (G-H) ELISA analysis of <t>REG3a</t> and Elafin in plasma of patient PB.
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    Overview plasma markers analyzed in the present study

    Journal: Medical Microbiology and Immunology

    Article Title: Inflammation, the kynurenines, and mucosal injury during human experimental enterotoxigenic Escherichia coli infection

    doi: 10.1007/s00430-024-00786-z

    Figure Lengend Snippet: Overview plasma markers analyzed in the present study

    Article Snippet: Plasma concentrations of regenerating islet-derived protein 3 alpha (Reg3A) were estimated using the Human Reg3A DuoSet ELISA kit (Catalog # DY5940-05; R&D Systems, Minneapolis, MN, USA) (Table ).

    Techniques: Clinical Proteomics, Concentration Assay, Activation Assay

    Decreased plasma ST2 and REG3A levels in aGvHD patients with WJMSC infusion. (a,b) ELISA of ST2 (a) and REG3A (b) in the plasma samples from aGvHD patients with the WJMSC infusion. Left, combined analysis; Middle, low‐dose (1.2 × 10 6 /kg WJMSCs); Right, high‐dose (10 × 10 6 /kg WJMSCs). Two cell infusion time points were indicated by red arrows (a,b). Data are mean ± s.e.m. and analysed by unpaired one‐tailed Student's t‐test (a,b). * P < 0.05, ** P < 0.01, *** P < 0.005

    Journal: Journal of Extracellular Vesicles

    Article Title: WJMSC‐derived small extracellular vesicle enhance T cell suppression through PD‐L1

    doi: 10.1002/jev2.12067

    Figure Lengend Snippet: Decreased plasma ST2 and REG3A levels in aGvHD patients with WJMSC infusion. (a,b) ELISA of ST2 (a) and REG3A (b) in the plasma samples from aGvHD patients with the WJMSC infusion. Left, combined analysis; Middle, low‐dose (1.2 × 10 6 /kg WJMSCs); Right, high‐dose (10 × 10 6 /kg WJMSCs). Two cell infusion time points were indicated by red arrows (a,b). Data are mean ± s.e.m. and analysed by unpaired one‐tailed Student's t‐test (a,b). * P < 0.05, ** P < 0.01, *** P < 0.005

    Article Snippet: For detection of IFN‐γ, ST2 and REG3 alpha (REG3A), plates were prepared using Duoset human IFN‐γ, ST2 and REG3A ELISA kit according to the manufacturers’ instructions (R&D systems, USA).

    Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, One-tailed Test

    Plasma analysis of inflammatory cytokines IL-2. (A) IL-6 (B) and IL-8 (C) in patient PB. Analysis of GVHD biomarkers ST-2 (D) , osteopontin (E) and follistatin (F) in patient PB. (G-H) ELISA analysis of REG3a and Elafin in plasma of patient PB.

    Journal: Oncotarget

    Article Title: Third party, umbilical cord blood derived regulatory T-cells for prevention of graft versus host disease in allogeneic hematopoietic stem cell transplantation: feasibility, safety and immune reconstitution

    doi: 10.18632/oncotarget.26242

    Figure Lengend Snippet: Plasma analysis of inflammatory cytokines IL-2. (A) IL-6 (B) and IL-8 (C) in patient PB. Analysis of GVHD biomarkers ST-2 (D) , osteopontin (E) and follistatin (F) in patient PB. (G-H) ELISA analysis of REG3a and Elafin in plasma of patient PB.

    Article Snippet: Elisa kits were obtained for Reg3a and Elafin from R&D Systems (Minneapolis, MN).

    Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay

    Plasma analysis of inflammatory cytokines IL-2. (A) IL-6 (B) and IL-8 (C) in patient PB. Analysis of GVHD biomarkers ST-2 (D) , osteopontin (E) and follistatin (F) in patient PB. (G-H) ELISA analysis of REG3a and Elafin in plasma of patient PB.

    Journal: Oncotarget

    Article Title: Third party, umbilical cord blood derived regulatory T-cells for prevention of graft versus host disease in allogeneic hematopoietic stem cell transplantation: feasibility, safety and immune reconstitution

    doi: 10.18632/oncotarget.26242

    Figure Lengend Snippet: Plasma analysis of inflammatory cytokines IL-2. (A) IL-6 (B) and IL-8 (C) in patient PB. Analysis of GVHD biomarkers ST-2 (D) , osteopontin (E) and follistatin (F) in patient PB. (G-H) ELISA analysis of REG3a and Elafin in plasma of patient PB.

    Article Snippet: Elisa kits were obtained for Reg3a and Elafin from R&D Systems (Minneapolis, MN).

    Techniques: Enzyme-linked Immunosorbent Assay