brightvision anti rabbit ap (Vector Laboratories)

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Vector Laboratories brightvision anti rabbit ap
Brightvision Anti Rabbit Ap, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1932 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/red+ap+substrate+kit/VECTOR+Red+Alkaline+Phosphatase+(Red+AP)+Substrate+Kit/10__22203_slash_ecm__v033a11-131-22-35
Average 96 stars, based on 1932 article reviews

brightvision anti rabbit ap - by Bioz Stars, 2026-10

96/100 stars

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Staining:

Article Title: Viral S protein histochemistry reveals few potential SARS-CoV-2 entry sites in human ocular tissues.
Article Snippet: Briefly, after blocking with 2 drops of Ultrablock (UltraVision Protein Block, Thermo Scientific, Germany), primary antibodies (100 μl, see Table 1) were incubated for 1 h at room temperature (RT) followed by 100 μl of biotinylated secondary antibodies applied for 30 min at RT (donkey anti-mouse #715-065-151 and donkey anti-rabbit #711-065-152, Jackson ImmunoResearch, Ely, UK). .. Next, one drop of streptavidin coupled with alkaline phosphatase for 30 min at RT (#5550-0002, SeraCare, Milford, MA, USA), and 150 μl of Vector Red AP Substrate Kit I for 8 min at RT (#SK-5100, Vector Labs, Burlingame, CA, USA) resulting in red staining. ..

Article Title: A synthetic lethality screen reveals ING5 as a genetic dependency of catalytically dead Set1A/COMPASS in mouse embryonic stem cells
Article Snippet: .. AP staining was conducted using the Red AP Substrate Kit (Vector Labs) following the manufacturer’s directions. .. The AP staining quantification in Supplemental Figure 2 was performed using the ColonyArea plugin (6) in the open-source image-processing ImageJ package FIJI, software version 2.1.0/1.53c.

Article Title: The decrease in peripheral blood basophils in a mouse model of IgE-induced inflammation involves their migration to lymph nodes.
Article Snippet: Background: During the active phase of urticaria, a decrease in peripheral blood basophils, known as basopenia, is observed.. We previously reported that basopenia occurs as a result of basophils migrating to the skin in a contact dermatitis model where a Th2 response is induced with oxazolone.. Objective: Although there is currently no established model for urticaria, given that urticaria is an IgEmediated immediate-type allergic reaction, we aimed to determine whether an IgE-mediated model could reproduce the decrease in basophils in peripheral blood observed during the active phase of urticaria.

Article Title: Kap1 regulates the self-renewal of embryonic stem cells and cellular reprogramming by modulating Oct4 protein stability.
Article Snippet: .. AP staining was performed for 30 min at room temperature in the dark using the Vector Red AP Substrate Kit I according to the manufacturer’s protocol. .. The cells were incubated with a substrate solution at room temperature until the development of suitable staining and were photographed with a model DFC300FX mounted digital camera (Leica, Solms, Germany).

Article Title: Role of the IgG4-related cholangitis autoantigen annexin A11 in cholangiocyte protection
Article Snippet: Sections were blocked for 8 minutes with Ultravision protein block (Thermo Scientific, Waltham, MS) and incubated overnight with anti-human annexin A11 primary antibody (Santa Cruz, Dallas, TX) 1:200 in antibody diluent (ScyTek, Logan, UT). .. After incubation with BrightVision Poly-horseradish peroxidase (HRP)-anti-mouse (Avantor, Radnor, PA), sections were stained with Red AP substrate kit (Vector, Burlingame, CA) and counterstained with hematoxylin. .. Microscopy was performed using Olympus BX51 (Olympus Corporation, Tokyo, Japan) at a 20x objective.

Article Title: Role of the IgG4-related cholangitis autoantigen annexin A11 in cholangiocyte protection.
Article Snippet: Sections were blocked for 8 minutes with Ultravision protein block (Thermo Scientific, Waltham, MS) and incubated overnight with anti-human annexin A11 primary antibody (Santa Cruz, Dallas, TX) 1:200 in antibody diluent (ScyTek, Logan, UT). .. After incubation with BrightVision Poly-horseradish peroxidase (HRP)anti-mouse (Avantor, Radnor, PA), sections were stained with Red AP substrate kit (Vector, Burlingame, CA) and counterstained with hematoxylin. .. Microscopy was performed using Olympus BX51 (Olympus Corporation, Tokyo, Japan) at a 20x objective.

other:

Article Title: One-Step Drug Screening System Utilizing Electrophysiological Activity in Multiple Brain Organoids.
Article Snippet: Cells were then incubated overnight at 4 °C with the following primary antibodies: anti-SOX2 (1:1000, AB5603, Millipore), anti-TRA-1-60 (1:200, MAB4360, Millipore), and anti-OCT4 (1:100, SC-9081, Santa Cruz Biotechnology).

Incubation:

Activity Assay:

Article Title: One‐Step Drug Screening System Utilizing Electrophysiological Activity in Multiple Brain Organoids
Article Snippet: .. Alkaline phosphatase (AP) activity in SCN2A‐mutant iPSCs was examined using the VECTOR Red AP Substrate Kit (SK‐5100, Vector Laboratories, Burlingame, CA, USA) according to the manufacturer's guidelines. ..

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