recombinant shp2 (R&D Systems)
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Structured Review
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recombinant shp2
Recombinant Shp2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+shp2/Recombinant+Human+Active+SHP-2+Protein%2C+CF/pm40419795-339-18-23
Average 93 stars, based on 8 article reviews
Recombinant Shp2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+shp2/Recombinant+Human+Active+SHP-2+Protein%2C+CF/pm40419795-339-18-23
Average 93 stars, based on 8 article reviews
recombinant shp2 - by Bioz Stars,
2026-10
93/100 stars
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1) Product Images from "Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells."
Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells.
Journal: Nature cell biology
doi: 10.1038/s41556-025-01663-4
Figure Legend Snippet: Fig. 5 | ITGB1 is a substrate for PTP-PEST and Shp2. a,b, A malachite green assay for free phosphate release after incubation of phosphorylated/non- phosphorylated ITGB1 peptides with recombinant Shp2 (n = 5 independent replicates, each performed in triplicate) (a) or PTP-PEST (n = 4 independent replicates, each performed in triplicate) (b). The significance was assessed using a Kruskal–Wallis test with a Dunn’s correction for multiple comparisons. The data are presented as the mean ± s.e.m. c,d, Schematics of FRET experiments (left) using mRuby2-tagged ITGB1 and Clover-tagged PTPs. Representative FLIM–FRET images (right) and quantification of apparent FRET efficiency of MM231 cells with stable expression of either ITGB1(WT)–mRuby2 or ITGB1(YYFF)–mRuby2
Techniques Used: Malachite Green Assay, Incubation, Recombinant, Expressing
Related Articles
Incubation:Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells. Article Snippet: IP The cells were collected in 300 μl IP lysis buffer (40 mM HEPES, 75 mM NaCl, 2 mM EDTA, 1% NP-40, along with protease (cOmpleteTM Mini, EDTA-free, Roche) and phosphatase (PhosSTOP, Roche) inhibitor cocktails) using a plastic cell lifter. Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells Article Snippet: For stable expression of the mRuby2-tagged ITGB1 WT and YYFF and the Illusia and Illusia(YYFF) intramolecular FRET biosensors, MM231 cells were cotransfected with the respective pPB.DEST vectors and pCMV-hyPBase (Supplementary Table ), using Lipofectamine 3000, as per the manufacturer’s protocol (Thermo Fisher Scientific). Recombinant:Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells. Article Snippet: IP The cells were collected in 300 μl IP lysis buffer (40 mM HEPES, 75 mM NaCl, 2 mM EDTA, 1% NP-40, along with protease (cOmpleteTM Mini, EDTA-free, Roche) and phosphatase (PhosSTOP, Roche) inhibitor cocktails) using a plastic cell lifter. Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells Article Snippet: For stable expression of the mRuby2-tagged ITGB1 WT and YYFF and the Illusia and Illusia(YYFF) intramolecular FRET biosensors, MM231 cells were cotransfected with the respective pPB.DEST vectors and pCMV-hyPBase (Supplementary Table ), using Lipofectamine 3000, as per the manufacturer’s protocol (Thermo Fisher Scientific). Malachite Green Assay:Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells. Article Snippet: IP The cells were collected in 300 μl IP lysis buffer (40 mM HEPES, 75 mM NaCl, 2 mM EDTA, 1% NP-40, along with protease (cOmpleteTM Mini, EDTA-free, Roche) and phosphatase (PhosSTOP, Roche) inhibitor cocktails) using a plastic cell lifter. Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells Article Snippet: For stable expression of the mRuby2-tagged ITGB1 WT and YYFF and the Illusia and Illusia(YYFF) intramolecular FRET biosensors, MM231 cells were cotransfected with the respective pPB.DEST vectors and pCMV-hyPBase (Supplementary Table ), using Lipofectamine 3000, as per the manufacturer’s protocol (Thermo Fisher Scientific). Expressing:Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells. Article Snippet: IP The cells were collected in 300 μl IP lysis buffer (40 mM HEPES, 75 mM NaCl, 2 mM EDTA, 1% NP-40, along with protease (cOmpleteTM Mini, EDTA-free, Roche) and phosphatase (PhosSTOP, Roche) inhibitor cocktails) using a plastic cell lifter. Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells Article Snippet: For stable expression of the mRuby2-tagged ITGB1 WT and YYFF and the Illusia and Illusia(YYFF) intramolecular FRET biosensors, MM231 cells were cotransfected with the respective pPB.DEST vectors and pCMV-hyPBase (Supplementary Table ), using Lipofectamine 3000, as per the manufacturer’s protocol (Thermo Fisher Scientific). Stable Transfection:Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells. Article Snippet: IP The cells were collected in 300 μl IP lysis buffer (40 mM HEPES, 75 mM NaCl, 2 mM EDTA, 1% NP-40, along with protease (cOmpleteTM Mini, EDTA-free, Roche) and phosphatase (PhosSTOP, Roche) inhibitor cocktails) using a plastic cell lifter. Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells Article Snippet: For stable expression of the mRuby2-tagged ITGB1 WT and YYFF and the Illusia and Illusia(YYFF) intramolecular FRET biosensors, MM231 cells were cotransfected with the respective pPB.DEST vectors and pCMV-hyPBase (Supplementary Table ), using Lipofectamine 3000, as per the manufacturer’s protocol (Thermo Fisher Scientific). Phospho-proteomics:Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells. Article Snippet: IP The cells were collected in 300 μl IP lysis buffer (40 mM HEPES, 75 mM NaCl, 2 mM EDTA, 1% NP-40, along with protease (cOmpleteTM Mini, EDTA-free, Roche) and phosphatase (PhosSTOP, Roche) inhibitor cocktails) using a plastic cell lifter. Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells Article Snippet: For stable expression of the mRuby2-tagged ITGB1 WT and YYFF and the Illusia and Illusia(YYFF) intramolecular FRET biosensors, MM231 cells were cotransfected with the respective pPB.DEST vectors and pCMV-hyPBase (Supplementary Table ), using Lipofectamine 3000, as per the manufacturer’s protocol (Thermo Fisher Scientific). Transfection:Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells. Article Snippet: IP The cells were collected in 300 μl IP lysis buffer (40 mM HEPES, 75 mM NaCl, 2 mM EDTA, 1% NP-40, along with protease (cOmpleteTM Mini, EDTA-free, Roche) and phosphatase (PhosSTOP, Roche) inhibitor cocktails) using a plastic cell lifter. Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells Article Snippet: For stable expression of the mRuby2-tagged ITGB1 WT and YYFF and the Illusia and Illusia(YYFF) intramolecular FRET biosensors, MM231 cells were cotransfected with the respective pPB.DEST vectors and pCMV-hyPBase (Supplementary Table ), using Lipofectamine 3000, as per the manufacturer’s protocol (Thermo Fisher Scientific). Control:Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells. Article Snippet: IP The cells were collected in 300 μl IP lysis buffer (40 mM HEPES, 75 mM NaCl, 2 mM EDTA, 1% NP-40, along with protease (cOmpleteTM Mini, EDTA-free, Roche) and phosphatase (PhosSTOP, Roche) inhibitor cocktails) using a plastic cell lifter. Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells Article Snippet: For stable expression of the mRuby2-tagged ITGB1 WT and YYFF and the Illusia and Illusia(YYFF) intramolecular FRET biosensors, MM231 cells were cotransfected with the respective pPB.DEST vectors and pCMV-hyPBase (Supplementary Table ), using Lipofectamine 3000, as per the manufacturer’s protocol (Thermo Fisher Scientific). Western Blot:Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells. Article Snippet: IP The cells were collected in 300 μl IP lysis buffer (40 mM HEPES, 75 mM NaCl, 2 mM EDTA, 1% NP-40, along with protease (cOmpleteTM Mini, EDTA-free, Roche) and phosphatase (PhosSTOP, Roche) inhibitor cocktails) using a plastic cell lifter. Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells Article Snippet: For stable expression of the mRuby2-tagged ITGB1 WT and YYFF and the Illusia and Illusia(YYFF) intramolecular FRET biosensors, MM231 cells were cotransfected with the respective pPB.DEST vectors and pCMV-hyPBase (Supplementary Table ), using Lipofectamine 3000, as per the manufacturer’s protocol (Thermo Fisher Scientific). Staining:Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells. Article Snippet: IP The cells were collected in 300 μl IP lysis buffer (40 mM HEPES, 75 mM NaCl, 2 mM EDTA, 1% NP-40, along with protease (cOmpleteTM Mini, EDTA-free, Roche) and phosphatase (PhosSTOP, Roche) inhibitor cocktails) using a plastic cell lifter. Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells Article Snippet: For stable expression of the mRuby2-tagged ITGB1 WT and YYFF and the Illusia and Illusia(YYFF) intramolecular FRET biosensors, MM231 cells were cotransfected with the respective pPB.DEST vectors and pCMV-hyPBase (Supplementary Table ), using Lipofectamine 3000, as per the manufacturer’s protocol (Thermo Fisher Scientific). Quantitative RT-PCR:Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells. Article Snippet: IP The cells were collected in 300 μl IP lysis buffer (40 mM HEPES, 75 mM NaCl, 2 mM EDTA, 1% NP-40, along with protease (cOmpleteTM Mini, EDTA-free, Roche) and phosphatase (PhosSTOP, Roche) inhibitor cocktails) using a plastic cell lifter. Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells Article Snippet: For stable expression of the mRuby2-tagged ITGB1 WT and YYFF and the Illusia and Illusia(YYFF) intramolecular FRET biosensors, MM231 cells were cotransfected with the respective pPB.DEST vectors and pCMV-hyPBase (Supplementary Table ), using Lipofectamine 3000, as per the manufacturer’s protocol (Thermo Fisher Scientific). Over Expression:Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells. Article Snippet: IP The cells were collected in 300 μl IP lysis buffer (40 mM HEPES, 75 mM NaCl, 2 mM EDTA, 1% NP-40, along with protease (cOmpleteTM Mini, EDTA-free, Roche) and phosphatase (PhosSTOP, Roche) inhibitor cocktails) using a plastic cell lifter. Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells Article Snippet: For stable expression of the mRuby2-tagged ITGB1 WT and YYFF and the Illusia and Illusia(YYFF) intramolecular FRET biosensors, MM231 cells were cotransfected with the respective pPB.DEST vectors and pCMV-hyPBase (Supplementary Table ), using Lipofectamine 3000, as per the manufacturer’s protocol (Thermo Fisher Scientific). Mutagenesis:Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells. Article Snippet: IP The cells were collected in 300 μl IP lysis buffer (40 mM HEPES, 75 mM NaCl, 2 mM EDTA, 1% NP-40, along with protease (cOmpleteTM Mini, EDTA-free, Roche) and phosphatase (PhosSTOP, Roche) inhibitor cocktails) using a plastic cell lifter. Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells Article Snippet: For stable expression of the mRuby2-tagged ITGB1 WT and YYFF and the Illusia and Illusia(YYFF) intramolecular FRET biosensors, MM231 cells were cotransfected with the respective pPB.DEST vectors and pCMV-hyPBase (Supplementary Table ), using Lipofectamine 3000, as per the manufacturer’s protocol (Thermo Fisher Scientific). Two Tailed Test:Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells. Article Snippet: IP The cells were collected in 300 μl IP lysis buffer (40 mM HEPES, 75 mM NaCl, 2 mM EDTA, 1% NP-40, along with protease (cOmpleteTM Mini, EDTA-free, Roche) and phosphatase (PhosSTOP, Roche) inhibitor cocktails) using a plastic cell lifter. Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells Article Snippet: For stable expression of the mRuby2-tagged ITGB1 WT and YYFF and the Illusia and Illusia(YYFF) intramolecular FRET biosensors, MM231 cells were cotransfected with the respective pPB.DEST vectors and pCMV-hyPBase (Supplementary Table ), using Lipofectamine 3000, as per the manufacturer’s protocol (Thermo Fisher Scientific). |