prmt1 (BPS Bioscience)

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BPS Bioscience prmt1
Prmt1, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+prmt1/PRMT1%2C+GST-tag+(E%2E+coli-derived)+Recombinant/10__1158_slash_1535___7163__mct___25___1105-43-5-14
Average 94 stars, based on 2 article reviews

prmt1 - by Bioz Stars, 2026-09

94/100 stars

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Modification:

Article Title: Target identification reveals protein arginine methyltransferase 1 is a potential target of phenyl vinyl sulfone and its derivatives
Article Snippet: .. High glucose Dulbecco’s Modified Eagle Medium, fetal bovine serum, Medium 199, OPTI-MEM, 0.25% trypsin-EDTA 1X (Gibco, Grand Island, NY); Immobilon Western chemiluminescent HRP substrate, C 18 Zip-tip (Millipore, Billerica, MA); phenyl vinyl sulfone, phenyl vinyl sulfonate, ethyl vinyl sulfone, BVT 948, NSC 95397, Bay 11-7082, Bay 11-9085, AMI-1 (Santa Cruz Biotechnology, Santa Cruz, CA); trypsin (Promega, Madison, WI); recombinant PRMT1, PRMT1 assay kit (BPS Bioscience, San Diego, CA); histone H4 (New England BioLabs, Ipswich, MA); recombinant glutathione reductase, glutathione reductase activity colorimetric assay Kit (BioVision, Milpitas, CA); antibodies: IMPDH1 (PA5-27792, Thermo Fisher Scientific Inc,Waltham, MA); Anti-dimethyl-Arginine Antibody, asymmetric(ASYM24) (07-414), Anti-dimethyl-Histone H4 (Arg3) Asymmetric Antibody(07-213-I), Anti-phosphotyrosine Antibody, 4G10® Platinum (05-1050) (Millipore, Billerica, MA); (Cell Signaling Technology, Danvers, MA); PRMT1 (B-2, sc-166963), PTP1B (D-4, sc-133259), glutathione reductase (C-10, sc-133245), vimentin (H-84, sc-5565) (Santa Cruz Biotechnology, Santa Cruz, CA); peroxidase-conjugated secondary antibodies, DylightTM 488, fluorescein (FITC)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA) were purchased from manufacturers indicated in parentheses. .. HeLa cells were obtained originally from American Type Culture Collection.

Article Title: Target identification reveals protein arginine methyltransferase 1 is a potential target of phenyl vinyl sulfone and its derivatives
Article Snippet: .. High glucose Dulbecco’s Modified Eagle Medium, fetal bovine serum, Medium 199, OPTI-MEM, 0.25% trypsin-EDTA 1X (Gibco, Grand Island, NY); Immobilon Western chemiluminescent HRP substrate, C18 Zip-tip (Millipore, Billerica, MA); phenyl vinyl sulfone, phenyl vinyl sulfonate, ethyl vinyl sulfone, BVT 948, NSC 95397, Bay 11-7082, Bay 11-9085, AMI-1 (Santa Cruz Biotechnology, Santa Cruz, CA); trypsin (Promega, Madison, WI); recombinant PRMT1, PRMT1 assay kit (BPS Bioscience, San Diego, CA); histone H4 (New England BioLabs, Ipswich, MA); recombinant glutathione reductase, glutathione reductase activity colorimetric assay Kit (BioVision, Milpitas, CA); antibodies: IMPDH1 (PA5-27792, Thermo Fisher Scientific Inc,Waltham, MA);Anti-dimethyl-Arginine Antibody,asymmetric(ASYM24) (07-414), Anti-dimethyl-Histone H4 (Arg3)Asymmetric Antibody(07-213-I), Anti-phosphotyrosine Antibody, 4G10® Platinum (05-1050) (Millipore, Billerica, MA); (Cell Signaling Technology, Danvers, MA); PRMT1 (B-2, sc-166963), PTP1B (D-4, sc-133259), glutathione reductase (C-10, sc-133245), vimentin (H-84, sc-5565) (Santa Cruz Biotechnology, Santa Cruz, CA); peroxidase-conjugated secondary antibodies, DylightTM 488, fluorescein (FITC)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA) were purchased from manufacturers indicated in parentheses. .. HeLa cells were obtained originally from American Type Culture Collection.

Western Blot:

Article Title: Target identification reveals protein arginine methyltransferase 1 is a potential target of phenyl vinyl sulfone and its derivatives
Article Snippet: .. High glucose Dulbecco’s Modified Eagle Medium, fetal bovine serum, Medium 199, OPTI-MEM, 0.25% trypsin-EDTA 1X (Gibco, Grand Island, NY); Immobilon Western chemiluminescent HRP substrate, C18 Zip-tip (Millipore, Billerica, MA); phenyl vinyl sulfone, phenyl vinyl sulfonate, ethyl vinyl sulfone, BVT 948, NSC 95397, Bay 11-7082, Bay 11-9085, AMI-1 (Santa Cruz Biotechnology, Santa Cruz, CA); trypsin (Promega, Madison, WI); recombinant PRMT1, PRMT1 assay kit (BPS Bioscience, San Diego, CA); histone H4 (New England BioLabs, Ipswich, MA); recombinant glutathione reductase, glutathione reductase activity colorimetric assay Kit (BioVision, Milpitas, CA); antibodies: IMPDH1 (PA5-27792, Thermo Fisher Scientific Inc,Waltham, MA);Anti-dimethyl-Arginine Antibody,asymmetric(ASYM24) (07-414), Anti-dimethyl-Histone H4 (Arg3)Asymmetric Antibody(07-213-I), Anti-phosphotyrosine Antibody, 4G10® Platinum (05-1050) (Millipore, Billerica, MA); (Cell Signaling Technology, Danvers, MA); PRMT1 (B-2, sc-166963), PTP1B (D-4, sc-133259), glutathione reductase (C-10, sc-133245), vimentin (H-84, sc-5565) (Santa Cruz Biotechnology, Santa Cruz, CA); peroxidase-conjugated secondary antibodies, DylightTM 488, fluorescein (FITC)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA) were purchased from manufacturers indicated in parentheses. .. HeLa cells were obtained originally from American Type Culture Collection.

Recombinant:

Article Title: Discovery of a Novel Chemotype of Histone Lysine Methyltransferase EHMT1/2 (GLP/G9a) Inhibitors: Rational Design, Synthesis, Biological Evaluation, and Co-crystal Structure
Article Snippet: .. Briefly, 3 L of human recombinant PRMT1 (BPS BioScience, # 51041) (final concentration 0.9 nM) were first incubated with 2.5 L of each compound for 30 min, then 4.5 L of a mixture of Histone H4 (1– Page 67 of 115 ACS Paragon Plus Environment Journal of Medicinal Chemistry 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 68 21) peptide biotinylated (AnaSpec, # 62555) (final concentration 100 nM) and of SAM (Sigma, # A7007) (final concentration 2 M) was added. .. The reaction was incubated for 60 min then it was stopped by the addition in subdued light of 5 L of anti-methyl- histone H4 arginine 3 (H4R3me) AlphaLISA acceptor beads (PerkinElmer, # AL150) (final concentration 20 g/mL) diluted in Epigenetic Buffer (PerkinElmer, # AL008).

Article Title: Discovery of a Novel Chemotype of Histone Lysine Methyltransferase EHMT1/2 (GLP/G9a) Inhibitors: Rational Design, Synthesis, Biological Evaluation, and Co-crystal Structure
Article Snippet: .. Briefly, 3 μ L of human recombinant PRMT1 (BPS BioScience, # 51041) (final concentration, 0.9 nM) was first incubated with 2.5 μ L of each compound for 30 min; then, 4.5 μ L of a mixture of histone H4 (1–21) peptide biotinylated (AnaSpec, # 62555) (final concentration, 100 nM) and SAM (Sigma, # A7007) (final concentration, 2 μ M) was added. .. The reaction was incubated for 60 min, and then it was stopped by the addition in subdued light of 5 μ L of anti-methyl-histone H4 arginine 3 (H4R3me) AlphaLISA acceptor beads (PerkinElmer, # AL150) (final concentration, 20 μ g/mL) diluted in epigenetic buffer (PerkinElmer, # AL008).

Article Title: Target identification reveals protein arginine methyltransferase 1 is a potential target of phenyl vinyl sulfone and its derivatives
Article Snippet: .. High glucose Dulbecco’s Modified Eagle Medium, fetal bovine serum, Medium 199, OPTI-MEM, 0.25% trypsin-EDTA 1X (Gibco, Grand Island, NY); Immobilon Western chemiluminescent HRP substrate, C18 Zip-tip (Millipore, Billerica, MA); phenyl vinyl sulfone, phenyl vinyl sulfonate, ethyl vinyl sulfone, BVT 948, NSC 95397, Bay 11-7082, Bay 11-9085, AMI-1 (Santa Cruz Biotechnology, Santa Cruz, CA); trypsin (Promega, Madison, WI); recombinant PRMT1, PRMT1 assay kit (BPS Bioscience, San Diego, CA); histone H4 (New England BioLabs, Ipswich, MA); recombinant glutathione reductase, glutathione reductase activity colorimetric assay Kit (BioVision, Milpitas, CA); antibodies: IMPDH1 (PA5-27792, Thermo Fisher Scientific Inc,Waltham, MA);Anti-dimethyl-Arginine Antibody,asymmetric(ASYM24) (07-414), Anti-dimethyl-Histone H4 (Arg3)Asymmetric Antibody(07-213-I), Anti-phosphotyrosine Antibody, 4G10® Platinum (05-1050) (Millipore, Billerica, MA); (Cell Signaling Technology, Danvers, MA); PRMT1 (B-2, sc-166963), PTP1B (D-4, sc-133259), glutathione reductase (C-10, sc-133245), vimentin (H-84, sc-5565) (Santa Cruz Biotechnology, Santa Cruz, CA); peroxidase-conjugated secondary antibodies, DylightTM 488, fluorescein (FITC)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA) were purchased from manufacturers indicated in parentheses. .. HeLa cells were obtained originally from American Type Culture Collection.

Article Title: Turning Nonselective Inhibitors of Type I Protein Arginine Methyltransferases into Potent and Selective Inhibitors of Protein Arginine Methyltransferase 4 through a Deconstruction–Reconstruction and Fragment-Growing Approach
Article Snippet: The assays were performed in white opaque OptiPlate-384 (PerkinElmer, #6007299) at 22 °C in a final volume of 25 μL, using the following assay buffer: 30 mM Tris–HCl pH 8.0, 1 mM DTT, 0.01% BSA, 0.01% Tween-20. .. In each well, 3 μL of human recombinant PRMT1 (BPS BioScience, #51041) (final concentration, 0.9 nM) was first incubated for 30 min with 2.5 μL of each compound (dissolved in DMSO and diluted in assay buffer to obtain 1% DMSO); then, 4.5 μL of a mixture of histone H4 (1–21) peptide biotinylated (AnaSpec, # 62555) (final concentration, 100 nM) and SAM (Sigma, #A7007) (final concentration, 2 μM) was added. .. The reaction was incubated for 60 min, and then it was stopped by the addition of 5 μL of antimethyl-histone H4 arginine 3 (H4R3me) AlphaLISA acceptor beads (PerkinElmer, #AL150) (final concentration, 20 μg/mL) diluted in Epigenetic Buffer 1X (PerkinElmer, #AL008).

Article Title: Turning Nonselective Inhibitors of Type I Protein Arginine Methyltransferases into Potent and Selective Inhibitors of Protein Arginine Methyltransferase 4 through a Deconstruction-Reconstruction and Fragment-Growing Approach.
Article Snippet: The assays were performed in white opaque OptiPlate-384 (PerkinElmer, #6007299) at 22 °C in a final volume of 25 μL, using the following assay buffer: 30 mM Tris−HCl pH 8.0, 1 mM DTT, 0.01% BSA, 0.01% Tween-20. .. In each well, 3 μL of human recombinant PRMT1 (BPS BioScience, #51041) (final concentration, 0.9 nM) was first incubated for 30 min with 2.5 μL of each compound (dissolved inDMSO and diluted in assay buffer to obtain 1% DMSO); then, 4.5 μL of a mixture of histone H4 (1−21) peptide biotinylated (AnaSpec, # 62555) (final concentration, 100 nM) and SAM (Sigma, #A7007) (final concentration, 2 μM) was added. .. The reaction was incubated for 60 min, and then it was stopped by the addition of 5 μL of antimethyl-histone H4 arginine 3 (H4R3me) AlphaLISA acceptor beads (PerkinElmer, #AL150) (final concentration, 20 μg/mL) diluted in Epigenetic Buffer 1X (PerkinElmer, #AL008).

Activity Assay:

Article Title: Target identification reveals protein arginine methyltransferase 1 is a potential target of phenyl vinyl sulfone and its derivatives
Article Snippet: .. High glucose Dulbecco’s Modified Eagle Medium, fetal bovine serum, Medium 199, OPTI-MEM, 0.25% trypsin-EDTA 1X (Gibco, Grand Island, NY); Immobilon Western chemiluminescent HRP substrate, C18 Zip-tip (Millipore, Billerica, MA); phenyl vinyl sulfone, phenyl vinyl sulfonate, ethyl vinyl sulfone, BVT 948, NSC 95397, Bay 11-7082, Bay 11-9085, AMI-1 (Santa Cruz Biotechnology, Santa Cruz, CA); trypsin (Promega, Madison, WI); recombinant PRMT1, PRMT1 assay kit (BPS Bioscience, San Diego, CA); histone H4 (New England BioLabs, Ipswich, MA); recombinant glutathione reductase, glutathione reductase activity colorimetric assay Kit (BioVision, Milpitas, CA); antibodies: IMPDH1 (PA5-27792, Thermo Fisher Scientific Inc,Waltham, MA);Anti-dimethyl-Arginine Antibody,asymmetric(ASYM24) (07-414), Anti-dimethyl-Histone H4 (Arg3)Asymmetric Antibody(07-213-I), Anti-phosphotyrosine Antibody, 4G10® Platinum (05-1050) (Millipore, Billerica, MA); (Cell Signaling Technology, Danvers, MA); PRMT1 (B-2, sc-166963), PTP1B (D-4, sc-133259), glutathione reductase (C-10, sc-133245), vimentin (H-84, sc-5565) (Santa Cruz Biotechnology, Santa Cruz, CA); peroxidase-conjugated secondary antibodies, DylightTM 488, fluorescein (FITC)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA) were purchased from manufacturers indicated in parentheses. .. HeLa cells were obtained originally from American Type Culture Collection.

Colorimetric Assay:

Article Title: Target identification reveals protein arginine methyltransferase 1 is a potential target of phenyl vinyl sulfone and its derivatives
Article Snippet: .. High glucose Dulbecco’s Modified Eagle Medium, fetal bovine serum, Medium 199, OPTI-MEM, 0.25% trypsin-EDTA 1X (Gibco, Grand Island, NY); Immobilon Western chemiluminescent HRP substrate, C18 Zip-tip (Millipore, Billerica, MA); phenyl vinyl sulfone, phenyl vinyl sulfonate, ethyl vinyl sulfone, BVT 948, NSC 95397, Bay 11-7082, Bay 11-9085, AMI-1 (Santa Cruz Biotechnology, Santa Cruz, CA); trypsin (Promega, Madison, WI); recombinant PRMT1, PRMT1 assay kit (BPS Bioscience, San Diego, CA); histone H4 (New England BioLabs, Ipswich, MA); recombinant glutathione reductase, glutathione reductase activity colorimetric assay Kit (BioVision, Milpitas, CA); antibodies: IMPDH1 (PA5-27792, Thermo Fisher Scientific Inc,Waltham, MA);Anti-dimethyl-Arginine Antibody,asymmetric(ASYM24) (07-414), Anti-dimethyl-Histone H4 (Arg3)Asymmetric Antibody(07-213-I), Anti-phosphotyrosine Antibody, 4G10® Platinum (05-1050) (Millipore, Billerica, MA); (Cell Signaling Technology, Danvers, MA); PRMT1 (B-2, sc-166963), PTP1B (D-4, sc-133259), glutathione reductase (C-10, sc-133245), vimentin (H-84, sc-5565) (Santa Cruz Biotechnology, Santa Cruz, CA); peroxidase-conjugated secondary antibodies, DylightTM 488, fluorescein (FITC)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA) were purchased from manufacturers indicated in parentheses. .. HeLa cells were obtained originally from American Type Culture Collection.

Concentration Assay:

Article Title: Discovery of a Novel Chemotype of Histone Lysine Methyltransferase EHMT1/2 (GLP/G9a) Inhibitors: Rational Design, Synthesis, Biological Evaluation, and Co-crystal Structure
Article Snippet: .. Briefly, 3 μ L of human recombinant PRMT1 (BPS BioScience, # 51041) (final concentration, 0.9 nM) was first incubated with 2.5 μ L of each compound for 30 min; then, 4.5 μ L of a mixture of histone H4 (1–21) peptide biotinylated (AnaSpec, # 62555) (final concentration, 100 nM) and SAM (Sigma, # A7007) (final concentration, 2 μ M) was added. .. The reaction was incubated for 60 min, and then it was stopped by the addition in subdued light of 5 μ L of anti-methyl-histone H4 arginine 3 (H4R3me) AlphaLISA acceptor beads (PerkinElmer, # AL150) (final concentration, 20 μ g/mL) diluted in epigenetic buffer (PerkinElmer, # AL008).

Article Title: Turning Nonselective Inhibitors of Type I Protein Arginine Methyltransferases into Potent and Selective Inhibitors of Protein Arginine Methyltransferase 4 through a Deconstruction-Reconstruction and Fragment-Growing Approach.
Article Snippet: The assays were performed in white opaque OptiPlate-384 (PerkinElmer, #6007299) at 22 °C in a final volume of 25 μL, using the following assay buffer: 30 mM Tris−HCl pH 8.0, 1 mM DTT, 0.01% BSA, 0.01% Tween-20. .. In each well, 3 μL of human recombinant PRMT1 (BPS BioScience, #51041) (final concentration, 0.9 nM) was first incubated for 30 min with 2.5 μL of each compound (dissolved inDMSO and diluted in assay buffer to obtain 1% DMSO); then, 4.5 μL of a mixture of histone H4 (1−21) peptide biotinylated (AnaSpec, # 62555) (final concentration, 100 nM) and SAM (Sigma, #A7007) (final concentration, 2 μM) was added. .. The reaction was incubated for 60 min, and then it was stopped by the addition of 5 μL of antimethyl-histone H4 arginine 3 (H4R3me) AlphaLISA acceptor beads (PerkinElmer, #AL150) (final concentration, 20 μg/mL) diluted in Epigenetic Buffer 1X (PerkinElmer, #AL008).

Incubation:

Article Title: Discovery of a Novel Chemotype of Histone Lysine Methyltransferase EHMT1/2 (GLP/G9a) Inhibitors: Rational Design, Synthesis, Biological Evaluation, and Co-crystal Structure
Article Snippet: .. Briefly, 3 μ L of human recombinant PRMT1 (BPS BioScience, # 51041) (final concentration, 0.9 nM) was first incubated with 2.5 μ L of each compound for 30 min; then, 4.5 μ L of a mixture of histone H4 (1–21) peptide biotinylated (AnaSpec, # 62555) (final concentration, 100 nM) and SAM (Sigma, # A7007) (final concentration, 2 μ M) was added. .. The reaction was incubated for 60 min, and then it was stopped by the addition in subdued light of 5 μ L of anti-methyl-histone H4 arginine 3 (H4R3me) AlphaLISA acceptor beads (PerkinElmer, # AL150) (final concentration, 20 μ g/mL) diluted in epigenetic buffer (PerkinElmer, # AL008).

Article Title: Turning Nonselective Inhibitors of Type I Protein Arginine Methyltransferases into Potent and Selective Inhibitors of Protein Arginine Methyltransferase 4 through a Deconstruction-Reconstruction and Fragment-Growing Approach.
Article Snippet: The assays were performed in white opaque OptiPlate-384 (PerkinElmer, #6007299) at 22 °C in a final volume of 25 μL, using the following assay buffer: 30 mM Tris−HCl pH 8.0, 1 mM DTT, 0.01% BSA, 0.01% Tween-20. .. In each well, 3 μL of human recombinant PRMT1 (BPS BioScience, #51041) (final concentration, 0.9 nM) was first incubated for 30 min with 2.5 μL of each compound (dissolved inDMSO and diluted in assay buffer to obtain 1% DMSO); then, 4.5 μL of a mixture of histone H4 (1−21) peptide biotinylated (AnaSpec, # 62555) (final concentration, 100 nM) and SAM (Sigma, #A7007) (final concentration, 2 μM) was added. .. The reaction was incubated for 60 min, and then it was stopped by the addition of 5 μL of antimethyl-histone H4 arginine 3 (H4R3me) AlphaLISA acceptor beads (PerkinElmer, #AL150) (final concentration, 20 μg/mL) diluted in Epigenetic Buffer 1X (PerkinElmer, #AL008).

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