recombinant mouse il7 (PeproTech)
Structured Review
Recombinant Mouse Il7, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il7/ccl21+chemokine/pm40249077-193-39-42
Average 90 stars, based on 1 article reviews
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Recombinant:Article Title: Glycoengineering-based anti-PD-1-iRGD peptide conjugate boosts antitumor efficacy through T cell engagement Article Snippet: FTY720 , MCE , HY-12005. .. Article Title: Signalling input from divergent pathways subverts B cell transformation. Article Snippet: Bone-marrow cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM, Gibco) with GlutaMAX supplemented with 20% FBS, 100 IU ml−1 penicillin, 100 μg ml−1 streptomycin and 50 μmol l−1 β-mercaptoethanol (Gibco). .. Bone-marrow cells, for IL7-dependent pro-B-cell culture, were cultured in full IMDM supplemented with 10 ng ml−1 Article Title: Combined Deletion of ZFP36L1 and ZFP36L2 Drives Superior Cytokine Production in T Cells at the Cost of Cell Fitness. Article Snippet: Erythrocytes were lysed using cold red blood cell lysis buffer (155 mM NH4Cl, 10 mM KHCO3, 0.1 mM EDTA, pH 7.4) for 60 s. OT-I T cells were purified with the MACS CD8a+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol. .. To generate effector OT-I T cells, purified OT-I T cells (1e6 cells/well) were activated with preseeded MEC.B7.SigOVA cells (1e5 cells/well) for 20 h. T cells were removed from stimulation and cultured in a complete medium supplemented with 10 ng/mL other:Article Title: Glycoengineering-based anti-PD-1-iRGD peptide conjugate boosts antitumor efficacy through T cell engagement. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse NRP-1-PerCP5.5 Biolegend Cat# 145208, RRID:AB_2562034 Anti-mouse PD-L1-PerCP5.5 Biolegend Cat# 124334, RRID:AB_2629831 Anti-mouse CD11b-FITC Biolegend Cat# 101206, RRID:AB_312788 Anti-mouse CD11b-APC Biolegend Cat# 101212, RRID:AB_312795 Anti-mouse CD11c-FITC Biolegend Cat# 117306, RRID:AB_313775 Anti-mouse F4/80-FITC Biolegend Cat# 123108, RRID:AB_893502 Anti-mouse MHC II-APC Biolegend Cat# 107614, RRID:AB_313329 Anti-mouse CD83-PE Biolegend Cat# 121508, RRID:AB_572015 Anti-mouse CD103-PerCP Biolegend Cat# 121406, RRID:AB_1133989 Anti-mouse CD86-PerCP Biolegend Cat# 105028, RRID:AB_893420 Anti-mouse CD163-PE Biolegend Cat# 156704, RRID:AB_2860724 Anti-mouse Ly6G-PE Biolegend Cat# 127608, RRID:AB_1186104 Anti-mouse Ly6G-PC7 Biolegend Cat# 127618, RRID:AB_1877262 Anti-mouse NRP-1 Biolegend Cat# 145201, RRID:AB_2561840 Chemicals, peptides, and recombinant proteins Recombinant Mouse PD-1 (HEK293, His) MCE HY-P73724 Recombinant human PD-1 (HEK293, His) MCE HY-P7396 Blinatumomab MCE HY-P9963 FTY720 MCE HY-12005 Recombinant mouse IL7 Peprotech #210-07 Cell Culture:Article Title: Signalling input from divergent pathways subverts B cell transformation. Article Snippet: Bone-marrow cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM, Gibco) with GlutaMAX supplemented with 20% FBS, 100 IU ml−1 penicillin, 100 μg ml−1 streptomycin and 50 μmol l−1 β-mercaptoethanol (Gibco). .. Bone-marrow cells, for IL7-dependent pro-B-cell culture, were cultured in full IMDM supplemented with 10 ng ml−1 Article Title: Combined Deletion of ZFP36L1 and ZFP36L2 Drives Superior Cytokine Production in T Cells at the Cost of Cell Fitness. Article Snippet: Erythrocytes were lysed using cold red blood cell lysis buffer (155 mM NH4Cl, 10 mM KHCO3, 0.1 mM EDTA, pH 7.4) for 60 s. OT-I T cells were purified with the MACS CD8a+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol. .. To generate effector OT-I T cells, purified OT-I T cells (1e6 cells/well) were activated with preseeded MEC.B7.SigOVA cells (1e5 cells/well) for 20 h. T cells were removed from stimulation and cultured in a complete medium supplemented with 10 ng/mL Purification:Article Title: Combined Deletion of ZFP36L1 and ZFP36L2 Drives Superior Cytokine Production in T Cells at the Cost of Cell Fitness. Article Snippet: Erythrocytes were lysed using cold red blood cell lysis buffer (155 mM NH4Cl, 10 mM KHCO3, 0.1 mM EDTA, pH 7.4) for 60 s. OT-I T cells were purified with the MACS CD8a+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol. .. To generate effector OT-I T cells, purified OT-I T cells (1e6 cells/well) were activated with preseeded MEC.B7.SigOVA cells (1e5 cells/well) for 20 h. T cells were removed from stimulation and cultured in a complete medium supplemented with 10 ng/mL |
