recombinant human fgf-basic (154 aa)  (PeproTech)

Journal: STAR Protocols

Article Title: Protocol for culturing neurospheres from progenitor cells in the dentate gyrus of aged mouse hippocampus

doi: 10.1016/j.xpro.2025.103692

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Article Snippet: Recombinant human FGF-basic (154 aa) , PeproTech , 100-18B.

Techniques: Recombinant, Microscopy, Electron Microscopy, Sterility, Cell Culture, Software

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Elucidation of the pluripotent potential of bovine embryonic lineages facilitates the establishment of formative stem cell lines

doi: 10.1007/s00018-024-05457-z

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Article Snippet: To prepare the 3i/LAF culture system, it is also necessary to add the following small molecule inhibitors or cytokines to BM: CHIR99021 (1 μM, Selleckchem, S1263), IWR-1-endo (0.5 μM, Selleckchem, S7086), WH-4–023 (1 μM, Selleckchem, S7565), recombinant human LIF (10 ng/mL, PeproTech, 300–05), recombinant human Activin A (25 ng/mL, PeproTech, 120-14E), and recombinant human FGF-basic (154 aa) (10 ng/mL, PeproTech, 100-18B).

Techniques: Recombinant, Knock-Out, Saline, Staining, Transfection, Plasmid Preparation, Software

Journal: iScience

Article Title: Selective regulation of chemosensitivity in glioblastoma by phosphatidylinositol 3-kinase beta

doi: 10.1016/j.isci.2024.109921

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Article Snippet: Human FGF-basic (FGF-2/bFGF) (154 aa) Animal-Free Recombinant Protein PeproTech® , Thermo Fisher Scientific , Cat#AF-100-18B-1MG.

Techniques: Recombinant, Transfection, Proliferation Assay, Concentration Assay, Software, Protein Array, Expressing, shRNA, Modification

Journal: Cell & Bioscience

Article Title: Phosphorylation at tyrosine 317 and 508 are crucial for PIK3CA/p110α to promote CRC tumorigenesis

doi: 10.1186/s13578-023-01102-7

Figure Lengend Snippet: p110α protein undergoes tyrosine phosphorylation. A Schematic of isogenic cell lines in which endogenous p110α was tagged with SBP-3×FLAG-6×HIS. ABD: adaptor-binding domain; RBD: Ras-binding domain; C2: C2 domain; helical: helical domain; kinase: kinase domain; SBP: SBP tag; FLAG: 3×FLAG tag; HIS: 6×HIS tag. B-C DLD1-p110α-SFH cells ( B ) or HCT116-p110α-SFH cells ( C ) were serum-starved overnight and then treated with or without pervanadate for 30 min. Cell lysates were immunoprecipitated with FLAG agarose beads and immunocomplex were blotted with indicated antibodies. DLD1-p110α-SFH: DLD1 isogenic cells in which endogenous p110α was tagged with SBP-3×FLAG-6×HIS; HCT116-p110α-SFH: HCT116 isogenic cells in which endogenous p110α was tagged with SBP-3×FLAG-6×HIS. D HCT116-p110α-SFH cells were serum-starved overnight and then treated with growth factors, insulin, or pervanadate for indicated time. Cell lysates were immunoprecipitated with anti-FLAG agarose beads and immunocomplex were blotted with the indicated antibodies. FGF: fibroblast growth factor; PDGF: platelet derived growth factor BB; HGF: hepatocyte growth factor; EGF: epidermal growth factor; Per: pervanadate. E Schematic of p110α tyrosine phosphorylation sites. Tyrosine 317 and 508 located in the linker regions RBD-C2 and C2- Helical of p110α respectively. F Mutation at Y317 and/or Y508 reduced tyrosine phosphorylation of p110α. Wild-type or mutant p110α constructs were transfected into 293T cells. Cells were serum-starved overnight and then treated with or without pervanadate for 30 min. Cell lysates were immunoprecipitated with FLAG agarose beads and immunocomplex were blotted with indicated antibodies

Article Snippet: EGF (cat# 8916) and FGF (cat# 61,977) were purchased from cell signaling technology (MA, USA).

Techniques: Phospho-proteomics, Binding Assay, Immunoprecipitation, Derivative Assay, Mutagenesis, Construct, Transfection