Journal: STAR Protocols

Article Title: Protocol for evaluating RNA-protein associations in mammalian cells with RIP-seq and RIP-qPCR

doi: 10.1016/j.xpro.2025.104298

Figure Lengend Snippet: Demonstration of trace or undetectable DNA contamination in RNA samples purified using TRIzol/chloroform extraction and DNase I digestion 5% input, SRSF1 RIP, and RBM15 RIP RNA samples were purified as described in Steps 38–43. cDNA samples were generated with or without reverse transcriptase and used as templates in qPCR reactions with primers targeting the Repeat A region of Xist RNA known to associate with the proteins SRSF1 and RBM15. , , , Repeat A signal is reported as percent of input, derived from a standard curve prepared by serially diluting of the 5% input +RT cDNA. Dots represent technical triplicate qPCR values, and average Cq values are reported. Related to Step 69.

Article Snippet: RBM15 , Proteintech , 10587-1-AP.

Techniques: Purification, Extraction, Generated, Reverse Transcription, Derivative Assay