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rbfox-1 antibody  (Merck KGaA)

 
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    Structured Review

    Merck KGaA rbfox-1 antibody
    Rbfox 1 Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rbfox-1/rbfox+1+antibody/pm34880207-272-17-19
    Average 90 stars, based on 1 article reviews
    rbfox-1 antibody - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Immunostaining:

    Article Title: MiRNA-132/212 regulates tight junction stabilization in blood–brain barrier after stroke
    Article Snippet: The following antibodies were used: CRTC1 (1:1000; Cell Signaling Technology, Danvers, MA, USA); TJAP-1 (1:1000; Novus Biologicals); RBFox-1 (1:1000; Merck Millipore); E-Cadherin (1:1000; Cell Signaling Technology); Claudin-1 (1:1000; Invitrogen); cleaved Caspase-3 (1:1000; Cell Signaling Technology); β-actin (1:5000; Proteintech, Rosemont, IL, USA).

    Lactate Dehydrogenase Assay:

    Article Title: MiRNA-132/212 regulates tight junction stabilization in blood–brain barrier after stroke
    Article Snippet: The following antibodies were used: CRTC1 (1:1000; Cell Signaling Technology, Danvers, MA, USA); TJAP-1 (1:1000; Novus Biologicals); RBFox-1 (1:1000; Merck Millipore); E-Cadherin (1:1000; Cell Signaling Technology); Claudin-1 (1:1000; Invitrogen); cleaved Caspase-3 (1:1000; Cell Signaling Technology); β-actin (1:5000; Proteintech, Rosemont, IL, USA).

    Western Blot:

    Article Title: MiRNA-132/212 regulates tight junction stabilization in blood–brain barrier after stroke
    Article Snippet: The following antibodies were used: CRTC1 (1:1000; Cell Signaling Technology, Danvers, MA, USA); TJAP-1 (1:1000; Novus Biologicals); RBFox-1 (1:1000; Merck Millipore); E-Cadherin (1:1000; Cell Signaling Technology); Claudin-1 (1:1000; Invitrogen); cleaved Caspase-3 (1:1000; Cell Signaling Technology); β-actin (1:5000; Proteintech, Rosemont, IL, USA).

    Luciferase:

    Article Title: MiRNA-132/212 regulates tight junction stabilization in blood–brain barrier after stroke
    Article Snippet: The following antibodies were used: CRTC1 (1:1000; Cell Signaling Technology, Danvers, MA, USA); TJAP-1 (1:1000; Novus Biologicals); RBFox-1 (1:1000; Merck Millipore); E-Cadherin (1:1000; Cell Signaling Technology); Claudin-1 (1:1000; Invitrogen); cleaved Caspase-3 (1:1000; Cell Signaling Technology); β-actin (1:5000; Proteintech, Rosemont, IL, USA).

    Activity Assay:

    Article Title: MiRNA-132/212 regulates tight junction stabilization in blood–brain barrier after stroke
    Article Snippet: The following antibodies were used: CRTC1 (1:1000; Cell Signaling Technology, Danvers, MA, USA); TJAP-1 (1:1000; Novus Biologicals); RBFox-1 (1:1000; Merck Millipore); E-Cadherin (1:1000; Cell Signaling Technology); Claudin-1 (1:1000; Invitrogen); cleaved Caspase-3 (1:1000; Cell Signaling Technology); β-actin (1:5000; Proteintech, Rosemont, IL, USA).

    Transfection:

    Article Title: MiRNA-132/212 regulates tight junction stabilization in blood–brain barrier after stroke
    Article Snippet: The following antibodies were used: CRTC1 (1:1000; Cell Signaling Technology, Danvers, MA, USA); TJAP-1 (1:1000; Novus Biologicals); RBFox-1 (1:1000; Merck Millipore); E-Cadherin (1:1000; Cell Signaling Technology); Claudin-1 (1:1000; Invitrogen); cleaved Caspase-3 (1:1000; Cell Signaling Technology); β-actin (1:5000; Proteintech, Rosemont, IL, USA).

    Expressing:

    Article Title: MiRNA-132/212 regulates tight junction stabilization in blood–brain barrier after stroke
    Article Snippet: The following antibodies were used: CRTC1 (1:1000; Cell Signaling Technology, Danvers, MA, USA); TJAP-1 (1:1000; Novus Biologicals); RBFox-1 (1:1000; Merck Millipore); E-Cadherin (1:1000; Cell Signaling Technology); Claudin-1 (1:1000; Invitrogen); cleaved Caspase-3 (1:1000; Cell Signaling Technology); β-actin (1:5000; Proteintech, Rosemont, IL, USA).



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    A Representative microtubule-associated protein 2 (MAP2) immunostaining images of CRTC1 KO and WT neurons, before and after OGD. (Scale Bar, 100 μm). B LDH assay of CRTC1 KO and WT neuron up to 48 h after ischemia/reperfusion. Representative Western Blot images ( C ) and quantification ( D ) of <t>RBFox-1</t> in CRTC1 KO and WT mice penumbra region. E Relative luciferase activity of CHO cell line co-transfected with RBFox-1 and miR-132 vehicle/mimic/antagomir. Representative Western Blot images ( F ) and quantification ( G ) of RBFox-1 in neurons transfected with miR-132 mimic/antagomir/vehicle, before and after OGD.
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    A Representative microtubule-associated protein 2 (MAP2) immunostaining images of CRTC1 KO and WT neurons, before and after OGD. (Scale Bar, 100 μm). B LDH assay of CRTC1 KO and WT neuron up to 48 h after ischemia/reperfusion. Representative Western Blot images ( C ) and quantification ( D ) of <t>RBFox-1</t> in CRTC1 KO and WT mice penumbra region. E Relative luciferase activity of CHO cell line co-transfected with RBFox-1 and miR-132 vehicle/mimic/antagomir. Representative Western Blot images ( F ) and quantification ( G ) of RBFox-1 in neurons transfected with miR-132 mimic/antagomir/vehicle, before and after OGD.
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    A Representative microtubule-associated protein 2 (MAP2) immunostaining images of CRTC1 KO and WT neurons, before and after OGD. (Scale Bar, 100 μm). B LDH assay of CRTC1 KO and WT neuron up to 48 h after ischemia/reperfusion. Representative Western Blot images ( C ) and quantification ( D ) of <t>RBFox-1</t> in CRTC1 KO and WT mice penumbra region. E Relative luciferase activity of CHO cell line co-transfected with RBFox-1 and miR-132 vehicle/mimic/antagomir. Representative Western Blot images ( F ) and quantification ( G ) of RBFox-1 in neurons transfected with miR-132 mimic/antagomir/vehicle, before and after OGD.
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    Image Search Results


    A Representative microtubule-associated protein 2 (MAP2) immunostaining images of CRTC1 KO and WT neurons, before and after OGD. (Scale Bar, 100 μm). B LDH assay of CRTC1 KO and WT neuron up to 48 h after ischemia/reperfusion. Representative Western Blot images ( C ) and quantification ( D ) of RBFox-1 in CRTC1 KO and WT mice penumbra region. E Relative luciferase activity of CHO cell line co-transfected with RBFox-1 and miR-132 vehicle/mimic/antagomir. Representative Western Blot images ( F ) and quantification ( G ) of RBFox-1 in neurons transfected with miR-132 mimic/antagomir/vehicle, before and after OGD.

    Journal: Cell Death Discovery

    Article Title: MiRNA-132/212 regulates tight junction stabilization in blood–brain barrier after stroke

    doi: 10.1038/s41420-021-00773-w

    Figure Lengend Snippet: A Representative microtubule-associated protein 2 (MAP2) immunostaining images of CRTC1 KO and WT neurons, before and after OGD. (Scale Bar, 100 μm). B LDH assay of CRTC1 KO and WT neuron up to 48 h after ischemia/reperfusion. Representative Western Blot images ( C ) and quantification ( D ) of RBFox-1 in CRTC1 KO and WT mice penumbra region. E Relative luciferase activity of CHO cell line co-transfected with RBFox-1 and miR-132 vehicle/mimic/antagomir. Representative Western Blot images ( F ) and quantification ( G ) of RBFox-1 in neurons transfected with miR-132 mimic/antagomir/vehicle, before and after OGD.

    Article Snippet: The following antibodies were used: CRTC1 (1:1000; Cell Signaling Technology, Danvers, MA, USA); TJAP-1 (1:1000; Novus Biologicals); RBFox-1 (1:1000; Merck Millipore); E-Cadherin (1:1000; Cell Signaling Technology); Claudin-1 (1:1000; Invitrogen); cleaved Caspase-3 (1:1000; Cell Signaling Technology); β-actin (1:5000; Proteintech, Rosemont, IL, USA).

    Techniques: Immunostaining, Lactate Dehydrogenase Assay, Western Blot, Luciferase, Activity Assay, Transfection

    CRTC1-mediated miR-132/212 attenuates neuron damages via suppressing RBFox-1, and alleviates blood–brain barrier dysfunction by regulating TJAP-1 and Claudin-1 expression in ischemic stroke.

    Journal: Cell Death Discovery

    Article Title: MiRNA-132/212 regulates tight junction stabilization in blood–brain barrier after stroke

    doi: 10.1038/s41420-021-00773-w

    Figure Lengend Snippet: CRTC1-mediated miR-132/212 attenuates neuron damages via suppressing RBFox-1, and alleviates blood–brain barrier dysfunction by regulating TJAP-1 and Claudin-1 expression in ischemic stroke.

    Article Snippet: The following antibodies were used: CRTC1 (1:1000; Cell Signaling Technology, Danvers, MA, USA); TJAP-1 (1:1000; Novus Biologicals); RBFox-1 (1:1000; Merck Millipore); E-Cadherin (1:1000; Cell Signaling Technology); Claudin-1 (1:1000; Invitrogen); cleaved Caspase-3 (1:1000; Cell Signaling Technology); β-actin (1:5000; Proteintech, Rosemont, IL, USA).

    Techniques: Expressing