murine raw264 7 cells (ATCC)

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ATCC murine raw264 7 cells
Murine Raw264 7 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24488 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/raw264+7+cells/RAW+264%2E7/10__2147_slash_jir__s633372-75-7-13
Average 99 stars, based on 24488 article reviews

murine raw264 7 cells - by Bioz Stars, 2026-10

99/100 stars

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Cell Culture:

Article Title: NSUN2 aggravates M1 macrophage polarization in arthritis progression through m5C modification of CCL2.
Article Snippet: Background: Arthritis is an inflammatory disease characterized by multifactorial pathogenesis in joints or surrounding tissues.. M1 macrophage polarization is a key pathological feature of arthritis, and m5C RNA modification has been implicated in disease progression.. This study aimed to elucidate the role of NSUN2, a writer of m5C modification, in modulating arthritis development.

Article Title: Amuc_1473 Links Gut Microbes to Skeletal Homeostasis and Counteracts Multifactorial Osteoporosis.
Article Snippet: These ells were maintained in α-Minimum Essential Medium ( αEM; Hyclone, Logan, USA) containing 15% fetal bovine serum FBS; Gibco, Grand Island, USA) and 1% penicillin–streptomycin PS; Cat. Solarbio, Beijing, China). .. RAW264.7 cells (ATCC; ockville, USA) were cultured in high-glucose Dulbecco’s modfied Eagle’s medium (DMEM; Gibco) with 10% FBS and 1% PS. ells were incubated at 37◦C and 5% CO2 . ..

Article Title: Hematopoietic PI3Kγ promotes abdominal aortic aneurysm by orchestrating macrophage trafficking.
Article Snippet: Background and purpose: Abdominal aortic aneurysm (AAA) is characterized by progressive aortic dilation, often driven by chronic inflammation.. PI3Kγ, predominantly expressed in myeloid cells, plays a pivotal role in mediating inflammatory responses in various cardiovascular conditions.. This study aimed to investigate whether, and through which mechanisms, PI3Kγ promotes AAA progression.

Enzyme-linked Immunosorbent Assay:

Article Title: Nanoengineered Macrophages for in vivo Three-Dimensional Photoacoustic Imaging and Immunotherapy of Acute Lung Injury
Article Snippet: Lipopolysaccharides (LPS) and Lumogallion (LG) were purchased from Beyotime Biotechnology Co., Ltd. Anti‐F4/80 and anti‐MPO monoclonal antibodies were obtained from Abcam Trading (Shanghai) Co., Ltd. .. The 3,3′‐diaminobenzidine (DAB) substrate kit was purchased from Fuzhou Maixin Biotech, Ltd. Enzyme‐linked immunosorbent assay (ELISA) kits for mouse cytokines were sourced from Wuhan Boster Biological Technology Co., Ltd. RAW264.7 cells were obtained from the American Type Culture Collection (ATCC, TIB-71TM, Manassas, VA, USA). ..

Incubation:

Article Title: BMSC-targeting aptamer conjugation enhances bone delivery of a rice bran peptide to mitigate osteoporosis via eIF3L
Article Snippet: MLO-Y4 cells (Procell) were maintained in α-MEM (Hyclone, Logan, USA) with 2.5% FBS, 2.5% calf serum (Gibco), and 1% PS. .. RAW264.7 cells (ATCC, TIB-71) were incubated in high glucose DMEM (Gibco) containing 10% FBS and 1% PS. ..

Modification:

Article Title: Bioactive potential of 70% ethanol extracts from different cheese products: LPS-stimulated macrophage cytokine response, radical-scavenging activity, and peptide characterization
Article Snippet: The protein concentrations of each cheese ExtractW1 were determined using a Qubit Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA), and the adjusted samples at 250 μg mL− 1 of bovine serum albumin (BSA)-equivalent were subjected to SDS-PAGE at 100 V. After electrophoresis, the gels were stained with Coomassie Brilliant Blue G-250 (Bio-Rad Laboratories, Hercules, CA, USA), and detection was performed using a Gel Imaging System (Fusion SL4, Vilber Lourmat, Marne-la-Vallée, France). .. RAW264.7 cells (TIB-71, American Type Culture Collection, Manassas, VA, USA) were routinely maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U mL− 1 penicillin, and 100 μg mL− 1 streptomycin in a humidified atmosphere of 5% CO2 at 37 ◦C. ..

Article Title: Substrate pre-stretch reprograms macrophage behavior through surface topographical remodeling
Article Snippet: .. RAW264.7 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and maintained in Advanced Dulbecco's Modified Eagle's Medium (DMEM; Invitrogen) supplemented with 10 % fetal bovine serum (FBS; Fisher Scientific, Houston, TX) and 1 % (v/v) penicillin-streptomycin antibiotic mixture. ..

other:

Article Title: Selective disruption of microtubule formation at the nuclear envelope impairs the bone resorption capacity of osteoclasts
Article Snippet: The following primary antibodies were used in this study: rabbit anti-AKAP6 (Sigma-Aldrich, HPA048741; 1:500), mouse anti-GM130 (BD Biosciences, 610823; 1:500), rabbit anti-PCNT (Abcam, ab220784; 1:500), mouse anti-tubulin-γ (Sigma-Aldrich, T6557; 1:200), rabbit anti-CDK5RAP2 (Abcam, ab70213; 1:500), rabbit anti-PCM1 (Proteintech, 19856-1-AP; 1:500), rat anti-tubulin-α (Abcam, ab6160; 1:500), rabbit anti-EB1 (Sigma-Aldrich, E3406; 1:500), mouse anti-nesprin-1 (Developmental Studies Hybridoma Bank, MANNES1E(8C3); 1:200), rabbit anti-SUN2C (Abcam, ab124916; 1:500), and mouse anti-lamin B1 (B-10) (Santa Cruz Biotechnology, sc-374015; 1:500).

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