elisa serum amyloid a saa  (BioVendor Instruments)

Journal: Nature metabolism

Article Title: Hippo-YAP/TAZ signalling coordinates adipose plasticity and energy balance by uncoupling leptin expression from fat mass.

doi: 10.1038/s42255-024-01045-4

Figure Lengend Snippet: Fig. 5 | Leptin upregulation is essential for the prevention of lipodystrophy- associated metabolic dysfunction in adipose-specific Lats1/Lats2 knockout mice. a, Serum leptin levels in Con and AKO mice (n = 5 Con, n = 5 AKO). b, Serum leptin levels in Con and Quad AKO (QKO) mice (n = 5 Con, n = 6 QKO). c, Serum leptin levels normalized by fat mass in Con and iAKO mice (n = 4 Con, n = 5 iAKO). d, RT–qPCR analysis of Adipoq and Lep expression in adipocytes differentiated

Article Snippet: Serum leptin (22-LEPMS-E01, ALPCO) and insulin (80-INSMSU-E10, ALPCO) levels were determined through ELISAs.

Techniques: Knock-Out, Quantitative RT-PCR, Expressing

Journal: Scientific reports

Article Title: Blockade of CXXC5-dishevelled interaction inhibits adipogenic differentiation, obesity, and insulin resistance in mice.

doi: 10.1038/s41598-022-25315-x

Figure Lengend Snippet: Figure 2. KY19334 treatment prevents diet-induced obesity and insulin resistance. C57BL/6 mice fed the NCD or HFD were orally administered with KY19334 or Orlistat concentration at 25 mg/kg/day for 8 weeks (n = 6 per group). (A) Representative photographs (upper panel) and MR images of mice (lower panel). Visceral and subcutaneous fat were quantified (B) Body weight changes. (C) Body weight gain (D) Daily food intake during all study weeks (mean ± SD., n.s indicates non-significance with HFD-fed vehicle group). (E) Plasma concentration of total cholesterol, HDL-cholesterol, leptin, and resistin in the overnight fasted state. (F) Fasting glucose. (G) Glucose and insulin tolerance test. Scale bar, 100 µm. Results are expressed as mean ± SD., n = 3 per group, *P < 0.05, ***P < 0.001, n.s indicates non-significance with HFD-fed vehicle group. NCD normal chow diet, HFD high-fat diet.

Article Snippet: ELISA assay kits were used to assess serum leptin (R&D system, MOB00B), serum resistin (R&D system, MRSN00), serum FFA (Cayman Chemical, 700310).

Techniques: Concentration Assay, Clinical Proteomics

Journal: Nutrients

Article Title: Bifidobacterium animalis ssp. lactis MG741 Reduces Body Weight and Ameliorates Nonalcoholic Fatty Liver Disease via Improving the Gut Permeability and Amelioration of Inflammatory Cytokines

doi: 10.3390/nu14091965

Figure Lengend Snippet: Bifidobacterium animalis ssp. lactis MG741 (MG741) ameliorates high-fat-diet-induced metabolic disorder parameters. ( A ) Fasting blood glucose; ( B ) insulin; ( C ) HOMA-IR; ( D ) serum ALT; ( E ) serum AST; ( F ) serum leptin; ( G ) serum total cholesterol; ( H ) serum HDL-cholesterol; ( I ) serum LDL-cholesterol; ( J ) liver weight; and ( K ) liver triglycerides. Data are presented as the mean ± standard error of the mean ( n = 9). The different letters (a–c) indicate significant differences ( p < 0.05) determined by one-way ANOVA with Tukey’s post hoc tests.

Article Snippet: Serum leptin concentration was determined using kits from R&D systems (MOB00B, Minneapolis, MN, USA), and insulin and endotoxin levels were determined with a kit from Thermo Fisher Scientific (EMINS, A39553).

Techniques: