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rat antimouse cd68 monoclonal antibody  (Bio-Rad)


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    Structured Review

    Bio-Rad rat antimouse cd68 monoclonal antibody
    Transcriptional analysis of the observed recovery of hippocampal function in . (A) Venn diagram depicting the number of transcripts differentially expressed 7 days after CA/CPR (left) or 30 days after CA/CPR (right). Lower venn diagram depicts the number of transcripts that were altered by CA/CPR at 7-day and recover by 30 days. (B) Bar graph depicting a total of 31 transcripts from (A) had greater than 2-fold change in expression. (C) STRING protein–protein interaction network of the 31 transcripts in (B) revealing <t>Cd68,</t> Spi1, and Trem2 as a central regulator of the altered transcripts. (D) Lollipop plot depicting the 10 pathways that are most affected by CA/CPR and subsequent recover by 30 days after CA/CPR.
    Rat Antimouse Cd68 Monoclonal Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3271 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+antimouse+cd68/Rat+anti+Mouse+CD68/pmc12084789-128-15-20
    Average 96 stars, based on 3271 article reviews
    rat antimouse cd68 monoclonal antibody - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Endogenous Recovery of Hippocampal Function Following Global Cerebral Ischemia in Juvenile Female Mice Is Influenced by Neuroinflammation and Circulating Sex Hormones"

    Article Title: Endogenous Recovery of Hippocampal Function Following Global Cerebral Ischemia in Juvenile Female Mice Is Influenced by Neuroinflammation and Circulating Sex Hormones

    Journal: Neural Plasticity

    doi: 10.1155/np/6103242

    Transcriptional analysis of the observed recovery of hippocampal function in . (A) Venn diagram depicting the number of transcripts differentially expressed 7 days after CA/CPR (left) or 30 days after CA/CPR (right). Lower venn diagram depicts the number of transcripts that were altered by CA/CPR at 7-day and recover by 30 days. (B) Bar graph depicting a total of 31 transcripts from (A) had greater than 2-fold change in expression. (C) STRING protein–protein interaction network of the 31 transcripts in (B) revealing Cd68, Spi1, and Trem2 as a central regulator of the altered transcripts. (D) Lollipop plot depicting the 10 pathways that are most affected by CA/CPR and subsequent recover by 30 days after CA/CPR.
    Figure Legend Snippet: Transcriptional analysis of the observed recovery of hippocampal function in . (A) Venn diagram depicting the number of transcripts differentially expressed 7 days after CA/CPR (left) or 30 days after CA/CPR (right). Lower venn diagram depicts the number of transcripts that were altered by CA/CPR at 7-day and recover by 30 days. (B) Bar graph depicting a total of 31 transcripts from (A) had greater than 2-fold change in expression. (C) STRING protein–protein interaction network of the 31 transcripts in (B) revealing Cd68, Spi1, and Trem2 as a central regulator of the altered transcripts. (D) Lollipop plot depicting the 10 pathways that are most affected by CA/CPR and subsequent recover by 30 days after CA/CPR.

    Techniques Used: Expressing

    Protein expression levels of CD68 mirror the transcriptional data. Representative images of DAPI (blue), Iba1 (red), and CD68 (green) staining in the CA1 region of the hippocampus in 7d Sham animals (A), 7d CA/CPR animals (B), 30d Sham animals (C), and 30d CA/CPR animals (D). Quantification of protein expression of CD68 from confocal images (E). ( ⁣ ∗∗∗ = p < 0.001, GLMM).
    Figure Legend Snippet: Protein expression levels of CD68 mirror the transcriptional data. Representative images of DAPI (blue), Iba1 (red), and CD68 (green) staining in the CA1 region of the hippocampus in 7d Sham animals (A), 7d CA/CPR animals (B), 30d Sham animals (C), and 30d CA/CPR animals (D). Quantification of protein expression of CD68 from confocal images (E). ( ⁣ ∗∗∗ = p < 0.001, GLMM).

    Techniques Used: Expressing, Staining

    GCI induced transcriptional changes associated with GCI induced post and presynaptic plasticity alterations. (A) CD68 mRNA is increased 7 days after CA/CPR with a recovery of expression to sham levels by 30 days post-CA/CPR. Removal of gonadal sex hormones allows CD68 expression levels to remain elevated 30 days after CA/CPR. ( ⁣ ∗ = p < 0.05, ⁣ ∗∗∗ = p < 0.001, One-way ANOVA) (B) N -type calcium channel transcripts are significantly elevated by CA/CPR at all time points despite recovery of hippocampal LTP. ( ⁣ ∗ = p < 0.05, One-way ANOVA).
    Figure Legend Snippet: GCI induced transcriptional changes associated with GCI induced post and presynaptic plasticity alterations. (A) CD68 mRNA is increased 7 days after CA/CPR with a recovery of expression to sham levels by 30 days post-CA/CPR. Removal of gonadal sex hormones allows CD68 expression levels to remain elevated 30 days after CA/CPR. ( ⁣ ∗ = p < 0.05, ⁣ ∗∗∗ = p < 0.001, One-way ANOVA) (B) N -type calcium channel transcripts are significantly elevated by CA/CPR at all time points despite recovery of hippocampal LTP. ( ⁣ ∗ = p < 0.05, One-way ANOVA).

    Techniques Used: Expressing

    Related Articles

    Incubation:

    Article Title: Crosstalk between cytotoxic CD8+ T cells and stressed cardiomyocytes triggers development of interstitial cardiac fibrosis in hypertensive mouse hearts.
    Article Snippet: Sections were sequentially incubated in 3% hydrogen peroxide in PBS (20 min), 10% normal serum/PBS (30 min) and biotin/avidin-blocking reagents (15 min each reagent) (Vector Laboratories). .. These sections were then incubated (1hr) with primary antibodies in serum: rat anti-mouse CD4 (BD: cat#550280), rat anti-mouse CD8 (BD: cat#550281), rat antimouse CD68 (BioRad: catMCA1957), rabbit anti-mouse TGFb1 (Novus Biological: cat#NB100-91995), or corresponding nonimmune IgG’s. .. Then, sections were washed and incubated with the appropriate secondary antibody (biotinylated mouse anti-rat: BD Pharmingen cat#550325 or biotinylated anti-rabbit; Vector Labs: cat#BA-1000)] for 40 min, followed by incubation with streptavidin horseradish peroxidase complex (30 min) (Vector Laboratories) and counterstaining with haematoxylin.

    Article Title: Social isolation produces a sex- and brain region-specific alteration of microglia state.
    Article Snippet: .. Another series of slices from the same brains were incubated with Iba1 as above together with rat antimouse CD68 (1:400, BioRad, RRID:AB_322219) and chicken anti-rat Alex647 (1:500, AB_2535875, ThermoFisher). ..

    other:

    Article Title: MicroRNA-disease Network Analysis Repurposes Methotrexate for the Treatment of Abdominal Aortic Aneurysm in Mice.
    Article Snippet: Rat antimouse CD68 (Catalog No. MCA1957) was purchased from AbD Serotec (Kidlington, UK).

    Article Title: MicroRNA–disease Network Analysis Repurposes Methotrexate for the Treatment of Abdominal Aortic Aneurysm in Mice
    Article Snippet: Rat antimouse CD68 (Catalog No. MCA1957) was purchased from AbD Serotec (Kidlington, UK).

    Article Title: Minocycline protects against microgliopathy in a Csf1r haplo-insufficient mouse model of adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP).
    Article Snippet: Rabbit anti-Iba1 (RRID: AB_2687911) was purchased from Wako and rat antimouse CD68 (RRID: AB_322219) was from Bio-Rad.

    Staining:

    Article Title: Flashes to Mind: Biological, imaging and machine learning analyses of radiation effects in preclinical mouse models
    Article Snippet: .. Immunofluoresence staining for microglia utilized rabbit anti-IBA-1 (1:500, Wako), rat antimouse CD68 (1:500 BioRad), goat anti-rabbit 488 (1:500, Invitrogen) and goat anti-rat 647 (1:1000, Abcam). .. Staining for neural stem cells, Sox2, an HMG box transcription factor utilized goat anti-SOX2 polyclonal (1:100, Novus) and donkey anti-goat 488 (1:200, Invitrogen).

    Immunofluorescence:

    Article Title: Extensive diet-induced atherosclerosis in scavenger receptor class B type 1-deficient mice is associated with substantial leukocytosis and elevated vascular cell adhesion molecule-1 expression in coronary artery endothelium.
    Article Snippet: Collagen was detected as blue-stained material in sections stained with Masson’s trichrome (Sigma, Oakville, ON, Canada) and quantified using the colour deconvolution and threshold functions in ImageJ software. .. CD68 and smooth muscle actin (SMA) were detected by immunofluorescence using rat antimouse CD68 (FA11, AbD Serotec, Cat # MCA 1957, Bio-Rad Laboratories, CA, United States; RRID:AB_322219) and rabbit antimouse SMA (Abcam Inc, Cat # ab5694, Cambridge MAUnited States; RRID:AB_2223021) primary antibodies and Alexa 594 goat anti-rat IgG (H + L) (Cat #A-11007; RRID:AB_10561522), and Alexa 488 goat anti-rabbit IgG (H + L) (Cat # A-11008; RRID:AB_143165) secondary antibodies (Life Technologies Inc, Burlington, Ontario Canada), respectively. ..



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    Transcriptional analysis of the observed recovery of hippocampal function in . (A) Venn diagram depicting the number of transcripts differentially expressed 7 days after CA/CPR (left) or 30 days after CA/CPR (right). Lower venn diagram depicts the number of transcripts that were altered by CA/CPR at 7-day and recover by 30 days. (B) Bar graph depicting a total of 31 transcripts from (A) had greater than 2-fold change in expression. (C) STRING protein–protein interaction network of the 31 transcripts in (B) revealing <t>Cd68,</t> Spi1, and Trem2 as a central regulator of the altered transcripts. (D) Lollipop plot depicting the 10 pathways that are most affected by CA/CPR and subsequent recover by 30 days after CA/CPR.
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    Transcriptional analysis of the observed recovery of hippocampal function in . (A) Venn diagram depicting the number of transcripts differentially expressed 7 days after CA/CPR (left) or 30 days after CA/CPR (right). Lower venn diagram depicts the number of transcripts that were altered by CA/CPR at 7-day and recover by 30 days. (B) Bar graph depicting a total of 31 transcripts from (A) had greater than 2-fold change in expression. (C) STRING protein–protein interaction network of the 31 transcripts in (B) revealing <t>Cd68,</t> Spi1, and Trem2 as a central regulator of the altered transcripts. (D) Lollipop plot depicting the 10 pathways that are most affected by CA/CPR and subsequent recover by 30 days after CA/CPR.
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    https://www.bioz.com/product/rat+antimouse+cd68/Rat+anti+Mouse+CD68/drayson_olivia__2024__flashes_to_mind_biological_imaging_and_machine_learning_analyses_of_radiation_effects_in_preclinical_mouse-535-9-13
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    Transcriptional analysis of the observed recovery of hippocampal function in . (A) Venn diagram depicting the number of transcripts differentially expressed 7 days after CA/CPR (left) or 30 days after CA/CPR (right). Lower venn diagram depicts the number of transcripts that were altered by CA/CPR at 7-day and recover by 30 days. (B) Bar graph depicting a total of 31 transcripts from (A) had greater than 2-fold change in expression. (C) STRING protein–protein interaction network of the 31 transcripts in (B) revealing <t>Cd68,</t> Spi1, and Trem2 as a central regulator of the altered transcripts. (D) Lollipop plot depicting the 10 pathways that are most affected by CA/CPR and subsequent recover by 30 days after CA/CPR.
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    Transcriptional analysis of the observed recovery of hippocampal function in . (A) Venn diagram depicting the number of transcripts differentially expressed 7 days after CA/CPR (left) or 30 days after CA/CPR (right). Lower venn diagram depicts the number of transcripts that were altered by CA/CPR at 7-day and recover by 30 days. (B) Bar graph depicting a total of 31 transcripts from (A) had greater than 2-fold change in expression. (C) STRING protein–protein interaction network of the 31 transcripts in (B) revealing <t>Cd68,</t> Spi1, and Trem2 as a central regulator of the altered transcripts. (D) Lollipop plot depicting the 10 pathways that are most affected by CA/CPR and subsequent recover by 30 days after CA/CPR.
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    Bio-Rad rat antimouse antibodies against cd68
    Transcriptional analysis of the observed recovery of hippocampal function in . (A) Venn diagram depicting the number of transcripts differentially expressed 7 days after CA/CPR (left) or 30 days after CA/CPR (right). Lower venn diagram depicts the number of transcripts that were altered by CA/CPR at 7-day and recover by 30 days. (B) Bar graph depicting a total of 31 transcripts from (A) had greater than 2-fold change in expression. (C) STRING protein–protein interaction network of the 31 transcripts in (B) revealing <t>Cd68,</t> Spi1, and Trem2 as a central regulator of the altered transcripts. (D) Lollipop plot depicting the 10 pathways that are most affected by CA/CPR and subsequent recover by 30 days after CA/CPR.
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    Bio-Rad rat antimouse cd68 antibody
    Transcriptional analysis of the observed recovery of hippocampal function in . (A) Venn diagram depicting the number of transcripts differentially expressed 7 days after CA/CPR (left) or 30 days after CA/CPR (right). Lower venn diagram depicts the number of transcripts that were altered by CA/CPR at 7-day and recover by 30 days. (B) Bar graph depicting a total of 31 transcripts from (A) had greater than 2-fold change in expression. (C) STRING protein–protein interaction network of the 31 transcripts in (B) revealing <t>Cd68,</t> Spi1, and Trem2 as a central regulator of the altered transcripts. (D) Lollipop plot depicting the 10 pathways that are most affected by CA/CPR and subsequent recover by 30 days after CA/CPR.
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    Image Search Results


    Transcriptional analysis of the observed recovery of hippocampal function in . (A) Venn diagram depicting the number of transcripts differentially expressed 7 days after CA/CPR (left) or 30 days after CA/CPR (right). Lower venn diagram depicts the number of transcripts that were altered by CA/CPR at 7-day and recover by 30 days. (B) Bar graph depicting a total of 31 transcripts from (A) had greater than 2-fold change in expression. (C) STRING protein–protein interaction network of the 31 transcripts in (B) revealing Cd68, Spi1, and Trem2 as a central regulator of the altered transcripts. (D) Lollipop plot depicting the 10 pathways that are most affected by CA/CPR and subsequent recover by 30 days after CA/CPR.

    Journal: Neural Plasticity

    Article Title: Endogenous Recovery of Hippocampal Function Following Global Cerebral Ischemia in Juvenile Female Mice Is Influenced by Neuroinflammation and Circulating Sex Hormones

    doi: 10.1155/np/6103242

    Figure Lengend Snippet: Transcriptional analysis of the observed recovery of hippocampal function in . (A) Venn diagram depicting the number of transcripts differentially expressed 7 days after CA/CPR (left) or 30 days after CA/CPR (right). Lower venn diagram depicts the number of transcripts that were altered by CA/CPR at 7-day and recover by 30 days. (B) Bar graph depicting a total of 31 transcripts from (A) had greater than 2-fold change in expression. (C) STRING protein–protein interaction network of the 31 transcripts in (B) revealing Cd68, Spi1, and Trem2 as a central regulator of the altered transcripts. (D) Lollipop plot depicting the 10 pathways that are most affected by CA/CPR and subsequent recover by 30 days after CA/CPR.

    Article Snippet: Slices were then incubated in primary goat antimouse Iba1 polyclonal antibody (Abcam, Waltham, MA) and rat antimouse CD68 monoclonal antibody (Bio-Rad, Hercules, CA) at 1:500 in 3% PBST solution overnight at 4°C.

    Techniques: Expressing

    Protein expression levels of CD68 mirror the transcriptional data. Representative images of DAPI (blue), Iba1 (red), and CD68 (green) staining in the CA1 region of the hippocampus in 7d Sham animals (A), 7d CA/CPR animals (B), 30d Sham animals (C), and 30d CA/CPR animals (D). Quantification of protein expression of CD68 from confocal images (E). ( ⁣ ∗∗∗ = p < 0.001, GLMM).

    Journal: Neural Plasticity

    Article Title: Endogenous Recovery of Hippocampal Function Following Global Cerebral Ischemia in Juvenile Female Mice Is Influenced by Neuroinflammation and Circulating Sex Hormones

    doi: 10.1155/np/6103242

    Figure Lengend Snippet: Protein expression levels of CD68 mirror the transcriptional data. Representative images of DAPI (blue), Iba1 (red), and CD68 (green) staining in the CA1 region of the hippocampus in 7d Sham animals (A), 7d CA/CPR animals (B), 30d Sham animals (C), and 30d CA/CPR animals (D). Quantification of protein expression of CD68 from confocal images (E). ( ⁣ ∗∗∗ = p < 0.001, GLMM).

    Article Snippet: Slices were then incubated in primary goat antimouse Iba1 polyclonal antibody (Abcam, Waltham, MA) and rat antimouse CD68 monoclonal antibody (Bio-Rad, Hercules, CA) at 1:500 in 3% PBST solution overnight at 4°C.

    Techniques: Expressing, Staining

    GCI induced transcriptional changes associated with GCI induced post and presynaptic plasticity alterations. (A) CD68 mRNA is increased 7 days after CA/CPR with a recovery of expression to sham levels by 30 days post-CA/CPR. Removal of gonadal sex hormones allows CD68 expression levels to remain elevated 30 days after CA/CPR. ( ⁣ ∗ = p < 0.05, ⁣ ∗∗∗ = p < 0.001, One-way ANOVA) (B) N -type calcium channel transcripts are significantly elevated by CA/CPR at all time points despite recovery of hippocampal LTP. ( ⁣ ∗ = p < 0.05, One-way ANOVA).

    Journal: Neural Plasticity

    Article Title: Endogenous Recovery of Hippocampal Function Following Global Cerebral Ischemia in Juvenile Female Mice Is Influenced by Neuroinflammation and Circulating Sex Hormones

    doi: 10.1155/np/6103242

    Figure Lengend Snippet: GCI induced transcriptional changes associated with GCI induced post and presynaptic plasticity alterations. (A) CD68 mRNA is increased 7 days after CA/CPR with a recovery of expression to sham levels by 30 days post-CA/CPR. Removal of gonadal sex hormones allows CD68 expression levels to remain elevated 30 days after CA/CPR. ( ⁣ ∗ = p < 0.05, ⁣ ∗∗∗ = p < 0.001, One-way ANOVA) (B) N -type calcium channel transcripts are significantly elevated by CA/CPR at all time points despite recovery of hippocampal LTP. ( ⁣ ∗ = p < 0.05, One-way ANOVA).

    Article Snippet: Slices were then incubated in primary goat antimouse Iba1 polyclonal antibody (Abcam, Waltham, MA) and rat antimouse CD68 monoclonal antibody (Bio-Rad, Hercules, CA) at 1:500 in 3% PBST solution overnight at 4°C.

    Techniques: Expressing