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factor κb ligand  (R&D Systems)


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    Structured Review

    R&D Systems factor κb ligand
    Factor κb Ligand, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rank+l/Recombinant+Mouse+TRANCE%2FRANK+L%2FTNFSF11+(E%2E+coli-expressed)/pmc13085024-92-28-32
    Average 96 stars, based on 297 article reviews
    factor κb ligand - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Selective disruption of microtubule formation at the nuclear envelope impairs the bone resorption capacity of osteoclasts.
    Article Snippet: For differentiation, cells were plated at 20.000 cells per ml in αMEM supplemented with 10% heat-inactivated FBS and 50 ng/ml of RANK-L (R&D Systems, 462-TEC-010).

    Article Title: Approximating bone ECM: Crosslinking directs individual and coupled osteoblast/osteoclast behavior.
    Article Snippet: Osteoblast and osteoclast communication (i.e. osteocoupling) is an intricate process, in which the biophysical profile of bone ECM is an aggregate product of their activities.. While the effect of microenvironmental cues on osteoblast and osteoclast maturation has been resolved into individual variables (e.g. stiffness or topography), a single cue can be limited with regards to reflecting the full biophysical scope of natural bone ECM.. Additionally, the natural modulation of bone ECM, which involves collagenous fibril and elastin crosslinking via lysyl oxidase, has yet to be reflected in current synthetic platforms.

    Article Title: Selective disruption of microtubule formation at the nuclear envelope impairs the bone resorption capacity of osteoclasts
    Article Snippet: For differentiation, cells were plated at 20,000 cells per ml in αMEM (Thermo Fisher Scientific, 41061029) supplemented with 10% heat-inactivated FBS and 50 ng/ml RANK-L (R&D Systems, 462-TEC-010).

    Control:

    Article Title: Inhibition of the histone demethylase KDM4B leads to activation of KDM1A, attenuates bacterial-induced pro-inflammatory cytokine release, and reduces osteoclastogenesis
    Article Snippet: Cells were supplemented with macrophage colony stimulating factor (M-CSF, R&D Systems, Cat# 416-ML-500) (15ng/mL/48h) for 3 days followed by supplementing with M-CSF (15 ng/mL/48 h) and receptor activator of nuclear factor kappa b ligand (RANK-L) (R&D Systems, Cat# 462-TEC-010) (50ng/mL/48h) for 2 days. .. On day 5, wells were rinsed to remove RANK-L and replacement media was supplemented with M-CSF (15 ng/mL) with or without Aa -LPS (100 ng/mL) or RANK-L (R&D Systems, Cat# 462-TEC-010) (50 ng/mL) and ML324 (SelleckChem, Cat# S7296, 10μM) or DMSO vehicle control for 72 hours. .. Cells were rinsed twice, fixed with 10% glutaraldehyde (Fisher, Cat# O2957-1) and stained for tartrate resistant acid phosphatase (TRAP) as described in BD Bioscience Technical Bulletin No. 445 using a 10-minute incubation with TRAP buffer.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Comparison of maternal isocaloric high carbohydrate and high fat diets on osteogenic and adipogenic genes expression in adolescent mice offspring
    Article Snippet: .. Serum OPG (R&D system; Cat ≠ DY459), RANK-L (R&D system; Cat ≠ MTR00), Lep (R&D system; Cat ≠ MOB00) and AdipoQ (EMD Millipore; Cat ≠ EZMADP-60 K) were measured by ELISA method, according to the manufacturer’s instructions. ..

    Recombinant:

    Article Title: Mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos
    Article Snippet: .. Human recombinant M-CSF (R&D Systems, Inc, Minneapolis, MN; Catalog Number 216-MCC) 40 ng/mL and RANK-L (R&D Systems, Inc; Catalog Number 390-TN) 400 ng /mL in combination were loaded into MSNPs by soaking overnight. ..



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    Image Search Results


    Dual inhibition of TGF-β and sclerostin increases bone mass in a hindlimb unloading model. a Experimental timeline of hindlimb unloading using C57B1/6 J mice and treatment with TGFβ-Ab, Scl-Ab, or both. Mice euthanized at week 12. b Representative μCT images of femoral diaphysis. c Representative confocal images of trabecular bone showing calcein (green), alizarin (red) signals, and DAPI (blue). Scale bar = 100 μm. d Representative images of TRAP-stained femur sections; violet = TRAP-positive, green = counterstain. Scale bar = 200 μm. e – h Bar plot of trabecular bone volume/total volume (Tra. BV/TV, e ), trabecular thickness (Tb. Th, f ), trabecular separation (Tb. Sp, g ), and trabecular number (Tb. N, h ) from μCT. Data = mean ± standard deviation. i , j Bar plot of mineral apposition rate (Tra. MAR, i ) and trabecular bone formation rate per bone surface (Tra. BFR/BS, j ) from labeling images. Data = mean ± standard deviation k Box plot of TRAP+ osteoclasts per bone surface (OC.N/BS), measured through ImageJ from TRAP-stained slides. l – n Box plot of serum P1NP ( l ) and TRAP ( m ) levels, and RANKL/OPG ratio ( n ). Group sizes: Control ( n = 5), Un_con ( n = 5), Scl-Ab ( n = 6), TGFβ-Ab ( n = 6), and Scl-Ab+TGFβ-Ab ( n = 7). Each point represents an individual mouse. ANOVA was used for statistics; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.000 1; ns not significant

    Journal: Bone Research

    Article Title: Spatially resolved osteoblast-traced transcriptomics uncovers TGF-β as a combination target with sclerostin in osteoporosis

    doi: 10.1038/s41413-026-00521-9

    Figure Lengend Snippet: Dual inhibition of TGF-β and sclerostin increases bone mass in a hindlimb unloading model. a Experimental timeline of hindlimb unloading using C57B1/6 J mice and treatment with TGFβ-Ab, Scl-Ab, or both. Mice euthanized at week 12. b Representative μCT images of femoral diaphysis. c Representative confocal images of trabecular bone showing calcein (green), alizarin (red) signals, and DAPI (blue). Scale bar = 100 μm. d Representative images of TRAP-stained femur sections; violet = TRAP-positive, green = counterstain. Scale bar = 200 μm. e – h Bar plot of trabecular bone volume/total volume (Tra. BV/TV, e ), trabecular thickness (Tb. Th, f ), trabecular separation (Tb. Sp, g ), and trabecular number (Tb. N, h ) from μCT. Data = mean ± standard deviation. i , j Bar plot of mineral apposition rate (Tra. MAR, i ) and trabecular bone formation rate per bone surface (Tra. BFR/BS, j ) from labeling images. Data = mean ± standard deviation k Box plot of TRAP+ osteoclasts per bone surface (OC.N/BS), measured through ImageJ from TRAP-stained slides. l – n Box plot of serum P1NP ( l ) and TRAP ( m ) levels, and RANKL/OPG ratio ( n ). Group sizes: Control ( n = 5), Un_con ( n = 5), Scl-Ab ( n = 6), TGFβ-Ab ( n = 6), and Scl-Ab+TGFβ-Ab ( n = 7). Each point represents an individual mouse. ANOVA was used for statistics; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.000 1; ns not significant

    Article Snippet: Serum levels of P1NP, CTx1, TRAcP, OPG, RANKL, and TGF-β1 were measured via an enzyme-linked immunosorbent assay (ELISA) performed using ELISA kit for mouse P1NP (AC-33F1, Immunodiagnostic Systems, UK), mouse CTx1 (AC-06F1, Immunodiagnostic Systems, UK), mouse TRAcP 5b (SB-TR103, Immunodiagnostic Systems, UK), mouse OPG (MOP00, R&D systems, USA), mouse RANKL (MTR00, R&D systems, USA), and mouse TGF-β1 (DY1679-05 and DY007B, R&D systems, USA) according to the manufacturer’s instruction.

    Techniques: Inhibition, Staining, Standard Deviation, Labeling, Control