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human burkitt s lymphoma ramos  (ATCC)


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    Structured Review

    ATCC human burkitt s lymphoma ramos
    KHK2840 is a novel human CD40 agonist. (A) Representative sensorgrams of hCD40 (left), cynomolgus monkey CD40 (middle left), rat CD40 (middle; right), and mouse CD40 (right) to KHK2840. KHK2840 was captured on the sensor chip immobilized with anti‐human IgG, and then dilutions of recombinant CD40 proteins (0.0625, 0.125, 0.250, 0.500, 1.00 μg/mL) were injected. (B) KHK2840 (red), isotype control (human anti‐DNP IgG2, gray), and positive control (human anti‐DNP IgG1, blue) bind to FcγRIIIa (left, 158F; middle, 158 V) and C1q. Data represent the mean ± SD of three experiments. (C) The in vitro expression of CD80, CD86, and IL‐12p70 in hmoDCs induced by KHK2840 (red) and isotype control (human anti‐DNP IgG2, gray). In IL‐12p70, values below the lower limit of quantification (LLOQ) were replaced with the LLOQ. Data represent the mean ± SD of three wells. The same experiments were repeated in a total of six donors. (D) The in vitro expression of CD86 and CD95 induced in <t>Ramos</t> cells by 0.01 (filled circle), 0.1 (blank circle), 1 (filled square) and 10 μg/mL (blank square) KHK2840 after 0.5, 4, 24, and 48 h of treatment. KHK2840 was washed out at the indicated time points. Cells were analyzed after a total of 48 h of incubation. Data represent the mean ± SD of three wells.
    Human Burkitt S Lymphoma Ramos, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ramos+ra+1/Ramos+(RA+1)%3B+Burkitt's+Lymphoma%3B+Human/pmc11611760-93-0-7
    Average 95 stars, based on 83 article reviews
    human burkitt s lymphoma ramos - by Bioz Stars, 2026-11
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    Images

    1) Product Images from "The novel and potent CD40 agonist KHK2840 augments the antitumor efficacy of anti‐ PD ‐1 antibody and paclitaxel"

    Article Title: The novel and potent CD40 agonist KHK2840 augments the antitumor efficacy of anti‐ PD ‐1 antibody and paclitaxel

    Journal: Cancer Science

    doi: 10.1111/cas.16366

    KHK2840 is a novel human CD40 agonist. (A) Representative sensorgrams of hCD40 (left), cynomolgus monkey CD40 (middle left), rat CD40 (middle; right), and mouse CD40 (right) to KHK2840. KHK2840 was captured on the sensor chip immobilized with anti‐human IgG, and then dilutions of recombinant CD40 proteins (0.0625, 0.125, 0.250, 0.500, 1.00 μg/mL) were injected. (B) KHK2840 (red), isotype control (human anti‐DNP IgG2, gray), and positive control (human anti‐DNP IgG1, blue) bind to FcγRIIIa (left, 158F; middle, 158 V) and C1q. Data represent the mean ± SD of three experiments. (C) The in vitro expression of CD80, CD86, and IL‐12p70 in hmoDCs induced by KHK2840 (red) and isotype control (human anti‐DNP IgG2, gray). In IL‐12p70, values below the lower limit of quantification (LLOQ) were replaced with the LLOQ. Data represent the mean ± SD of three wells. The same experiments were repeated in a total of six donors. (D) The in vitro expression of CD86 and CD95 induced in Ramos cells by 0.01 (filled circle), 0.1 (blank circle), 1 (filled square) and 10 μg/mL (blank square) KHK2840 after 0.5, 4, 24, and 48 h of treatment. KHK2840 was washed out at the indicated time points. Cells were analyzed after a total of 48 h of incubation. Data represent the mean ± SD of three wells.
    Figure Legend Snippet: KHK2840 is a novel human CD40 agonist. (A) Representative sensorgrams of hCD40 (left), cynomolgus monkey CD40 (middle left), rat CD40 (middle; right), and mouse CD40 (right) to KHK2840. KHK2840 was captured on the sensor chip immobilized with anti‐human IgG, and then dilutions of recombinant CD40 proteins (0.0625, 0.125, 0.250, 0.500, 1.00 μg/mL) were injected. (B) KHK2840 (red), isotype control (human anti‐DNP IgG2, gray), and positive control (human anti‐DNP IgG1, blue) bind to FcγRIIIa (left, 158F; middle, 158 V) and C1q. Data represent the mean ± SD of three experiments. (C) The in vitro expression of CD80, CD86, and IL‐12p70 in hmoDCs induced by KHK2840 (red) and isotype control (human anti‐DNP IgG2, gray). In IL‐12p70, values below the lower limit of quantification (LLOQ) were replaced with the LLOQ. Data represent the mean ± SD of three wells. The same experiments were repeated in a total of six donors. (D) The in vitro expression of CD86 and CD95 induced in Ramos cells by 0.01 (filled circle), 0.1 (blank circle), 1 (filled square) and 10 μg/mL (blank square) KHK2840 after 0.5, 4, 24, and 48 h of treatment. KHK2840 was washed out at the indicated time points. Cells were analyzed after a total of 48 h of incubation. Data represent the mean ± SD of three wells.

    Techniques Used: Recombinant, Injection, Control, Positive Control, In Vitro, Expressing, Incubation

    Related Articles

    Cell Culture:

    Article Title: Bruton's tyrosine kinase activity probe and method of using
    Article Snippet: .. Cell lines 293H (11631-017, Invitrogen), DOHH2 (ACC 47, DSMZ) and Ramos RA 1 (CRL-1596, ATCC) were routinely cultured in D-MEM and RPMI, respectively, supplemented with 10% FBS and non-essential amino acids (Invitrogen). .. Antibiotics blasticidin and G418 (Invitrogen) were included in the culture medium when necessary to select or to maintain transfected cells.

    Article Title: Potassium suppresses allosteric activation of ZAP-70-dependent T Cell Receptor signaling.
    Article Snippet: .. Jurkat E6.1 cells (ATCC # TIB-152), Jurkat P116 cells (ATCC # CRL-2676) and Ramos RA- 1(ATCC # CRL-1596) were cultured in high glucose RPMI 1640 media (GIBCO #A10491-01) and HEK293T cells were cultured in DMEM media (GIBCO #11995049), supplemented with 10%FBS (GIBCO #10270-106) and 1% anti-anti (GIBCO#15240-062) at 370C and 5% CO2. ..

    Transformation Assay:

    Article Title: B cell receptor modification in B cells
    Article Snippet: .. B Cell Engineering Optimal nucleofection parameters for Ramos RA 1, 2G6 (from ATCC) or EBV transformed polyclonal B cell lines were identified using a GFPmax (Lonza) plasmid as described for the Neon transfection System (Life Technologies). ..

    Article Title: In vitro study of Listeria monocytogenes infection to murine primary and human transformed B cells.
    Article Snippet: Immunity to Listeria monocytogenes is largely mediated by T lymphocytes.. Recently, B lymphocytes or their secreted products are implicated to provide immunity against L. monocytogenes infection.. To understand whether L. monocytogenes can infect and kill B cells as a possible strategy to initiate an infection, we examined the effects of L. monocytogenes on a human B lymphoma (Ramos RA-1) and mouse primary B cells in vitro.

    Plasmid Preparation:

    Article Title: B cell receptor modification in B cells
    Article Snippet: .. B Cell Engineering Optimal nucleofection parameters for Ramos RA 1, 2G6 (from ATCC) or EBV transformed polyclonal B cell lines were identified using a GFPmax (Lonza) plasmid as described for the Neon transfection System (Life Technologies). ..

    Transfection:

    Article Title: B cell receptor modification in B cells
    Article Snippet: .. B Cell Engineering Optimal nucleofection parameters for Ramos RA 1, 2G6 (from ATCC) or EBV transformed polyclonal B cell lines were identified using a GFPmax (Lonza) plasmid as described for the Neon transfection System (Life Technologies). ..

    Electrical Source Imaging:

    Article Title: Gold nanoparticle-streptavidin conjugates for rapid and efficient screening of aptamer function in lateral flow sensors using novel CD4-binding aptamers identified through Crossover-SELEX.
    Article Snippet: To decrease the burden of laborious and reagent-intensive screening of modified aptamers, their binding function requires assessment in assay formats compatible with the end diagnostic application.. Here, we report on the use of an alternative and cost-effective approach: a rapid lateral flow assay (LFA) utilising streptavidin-conjugated gold nanoparticles (AuNP) as reporter molecules to screen novel ssDNA aptamers for their ability to detect CD4.. Crossover-SELEX was employed to identify CD4-targeting aptamers from a ssDNA library enriched against a recombinant human CD4 protein (hCD4) conjugated to magneticbeads and to endogenous CD4 expressed by U937 cells.



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    ATCC human burkitt s lymphoma ramos
    KHK2840 is a novel human CD40 agonist. (A) Representative sensorgrams of hCD40 (left), cynomolgus monkey CD40 (middle left), rat CD40 (middle; right), and mouse CD40 (right) to KHK2840. KHK2840 was captured on the sensor chip immobilized with anti‐human IgG, and then dilutions of recombinant CD40 proteins (0.0625, 0.125, 0.250, 0.500, 1.00 μg/mL) were injected. (B) KHK2840 (red), isotype control (human anti‐DNP IgG2, gray), and positive control (human anti‐DNP IgG1, blue) bind to FcγRIIIa (left, 158F; middle, 158 V) and C1q. Data represent the mean ± SD of three experiments. (C) The in vitro expression of CD80, CD86, and IL‐12p70 in hmoDCs induced by KHK2840 (red) and isotype control (human anti‐DNP IgG2, gray). In IL‐12p70, values below the lower limit of quantification (LLOQ) were replaced with the LLOQ. Data represent the mean ± SD of three wells. The same experiments were repeated in a total of six donors. (D) The in vitro expression of CD86 and CD95 induced in <t>Ramos</t> cells by 0.01 (filled circle), 0.1 (blank circle), 1 (filled square) and 10 μg/mL (blank square) KHK2840 after 0.5, 4, 24, and 48 h of treatment. KHK2840 was washed out at the indicated time points. Cells were analyzed after a total of 48 h of incubation. Data represent the mean ± SD of three wells.
    Human Burkitt S Lymphoma Ramos, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ramos+ra+1/Ramos+(RA+1)%3B+Burkitt's+Lymphoma%3B+Human/pmc11611760-93-0-7
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    KHK2840 is a novel human CD40 agonist. (A) Representative sensorgrams of hCD40 (left), cynomolgus monkey CD40 (middle left), rat CD40 (middle; right), and mouse CD40 (right) to KHK2840. KHK2840 was captured on the sensor chip immobilized with anti‐human IgG, and then dilutions of recombinant CD40 proteins (0.0625, 0.125, 0.250, 0.500, 1.00 μg/mL) were injected. (B) KHK2840 (red), isotype control (human anti‐DNP IgG2, gray), and positive control (human anti‐DNP IgG1, blue) bind to FcγRIIIa (left, 158F; middle, 158 V) and C1q. Data represent the mean ± SD of three experiments. (C) The in vitro expression of CD80, CD86, and IL‐12p70 in hmoDCs induced by KHK2840 (red) and isotype control (human anti‐DNP IgG2, gray). In IL‐12p70, values below the lower limit of quantification (LLOQ) were replaced with the LLOQ. Data represent the mean ± SD of three wells. The same experiments were repeated in a total of six donors. (D) The in vitro expression of CD86 and CD95 induced in <t>Ramos</t> cells by 0.01 (filled circle), 0.1 (blank circle), 1 (filled square) and 10 μg/mL (blank square) KHK2840 after 0.5, 4, 24, and 48 h of treatment. KHK2840 was washed out at the indicated time points. Cells were analyzed after a total of 48 h of incubation. Data represent the mean ± SD of three wells.
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    ATCC ramos ra 1
    KHK2840 is a novel human CD40 agonist. (A) Representative sensorgrams of hCD40 (left), cynomolgus monkey CD40 (middle left), rat CD40 (middle; right), and mouse CD40 (right) to KHK2840. KHK2840 was captured on the sensor chip immobilized with anti‐human IgG, and then dilutions of recombinant CD40 proteins (0.0625, 0.125, 0.250, 0.500, 1.00 μg/mL) were injected. (B) KHK2840 (red), isotype control (human anti‐DNP IgG2, gray), and positive control (human anti‐DNP IgG1, blue) bind to FcγRIIIa (left, 158F; middle, 158 V) and C1q. Data represent the mean ± SD of three experiments. (C) The in vitro expression of CD80, CD86, and IL‐12p70 in hmoDCs induced by KHK2840 (red) and isotype control (human anti‐DNP IgG2, gray). In IL‐12p70, values below the lower limit of quantification (LLOQ) were replaced with the LLOQ. Data represent the mean ± SD of three wells. The same experiments were repeated in a total of six donors. (D) The in vitro expression of CD86 and CD95 induced in <t>Ramos</t> cells by 0.01 (filled circle), 0.1 (blank circle), 1 (filled square) and 10 μg/mL (blank square) KHK2840 after 0.5, 4, 24, and 48 h of treatment. KHK2840 was washed out at the indicated time points. Cells were analyzed after a total of 48 h of incubation. Data represent the mean ± SD of three wells.
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    ATCC ramos ra 1 cell line
    KHK2840 is a novel human CD40 agonist. (A) Representative sensorgrams of hCD40 (left), cynomolgus monkey CD40 (middle left), rat CD40 (middle; right), and mouse CD40 (right) to KHK2840. KHK2840 was captured on the sensor chip immobilized with anti‐human IgG, and then dilutions of recombinant CD40 proteins (0.0625, 0.125, 0.250, 0.500, 1.00 μg/mL) were injected. (B) KHK2840 (red), isotype control (human anti‐DNP IgG2, gray), and positive control (human anti‐DNP IgG1, blue) bind to FcγRIIIa (left, 158F; middle, 158 V) and C1q. Data represent the mean ± SD of three experiments. (C) The in vitro expression of CD80, CD86, and IL‐12p70 in hmoDCs induced by KHK2840 (red) and isotype control (human anti‐DNP IgG2, gray). In IL‐12p70, values below the lower limit of quantification (LLOQ) were replaced with the LLOQ. Data represent the mean ± SD of three wells. The same experiments were repeated in a total of six donors. (D) The in vitro expression of CD86 and CD95 induced in <t>Ramos</t> cells by 0.01 (filled circle), 0.1 (blank circle), 1 (filled square) and 10 μg/mL (blank square) KHK2840 after 0.5, 4, 24, and 48 h of treatment. KHK2840 was washed out at the indicated time points. Cells were analyzed after a total of 48 h of incubation. Data represent the mean ± SD of three wells.
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    ra 1  (ATCC)
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    ATCC ra 1
    KHK2840 is a novel human CD40 agonist. (A) Representative sensorgrams of hCD40 (left), cynomolgus monkey CD40 (middle left), rat CD40 (middle; right), and mouse CD40 (right) to KHK2840. KHK2840 was captured on the sensor chip immobilized with anti‐human IgG, and then dilutions of recombinant CD40 proteins (0.0625, 0.125, 0.250, 0.500, 1.00 μg/mL) were injected. (B) KHK2840 (red), isotype control (human anti‐DNP IgG2, gray), and positive control (human anti‐DNP IgG1, blue) bind to FcγRIIIa (left, 158F; middle, 158 V) and C1q. Data represent the mean ± SD of three experiments. (C) The in vitro expression of CD80, CD86, and IL‐12p70 in hmoDCs induced by KHK2840 (red) and isotype control (human anti‐DNP IgG2, gray). In IL‐12p70, values below the lower limit of quantification (LLOQ) were replaced with the LLOQ. Data represent the mean ± SD of three wells. The same experiments were repeated in a total of six donors. (D) The in vitro expression of CD86 and CD95 induced in <t>Ramos</t> cells by 0.01 (filled circle), 0.1 (blank circle), 1 (filled square) and 10 μg/mL (blank square) KHK2840 after 0.5, 4, 24, and 48 h of treatment. KHK2840 was washed out at the indicated time points. Cells were analyzed after a total of 48 h of incubation. Data represent the mean ± SD of three wells.
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    ra 1  (DSMZ)
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    DSMZ ra 1
    KHK2840 is a novel human CD40 agonist. (A) Representative sensorgrams of hCD40 (left), cynomolgus monkey CD40 (middle left), rat CD40 (middle; right), and mouse CD40 (right) to KHK2840. KHK2840 was captured on the sensor chip immobilized with anti‐human IgG, and then dilutions of recombinant CD40 proteins (0.0625, 0.125, 0.250, 0.500, 1.00 μg/mL) were injected. (B) KHK2840 (red), isotype control (human anti‐DNP IgG2, gray), and positive control (human anti‐DNP IgG1, blue) bind to FcγRIIIa (left, 158F; middle, 158 V) and C1q. Data represent the mean ± SD of three experiments. (C) The in vitro expression of CD80, CD86, and IL‐12p70 in hmoDCs induced by KHK2840 (red) and isotype control (human anti‐DNP IgG2, gray). In IL‐12p70, values below the lower limit of quantification (LLOQ) were replaced with the LLOQ. Data represent the mean ± SD of three wells. The same experiments were repeated in a total of six donors. (D) The in vitro expression of CD86 and CD95 induced in <t>Ramos</t> cells by 0.01 (filled circle), 0.1 (blank circle), 1 (filled square) and 10 μg/mL (blank square) KHK2840 after 0.5, 4, 24, and 48 h of treatment. KHK2840 was washed out at the indicated time points. Cells were analyzed after a total of 48 h of incubation. Data represent the mean ± SD of three wells.
    Ra 1, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    ATCC ramos cells
    Fig. 2. SARS-CoV-2 displayed on lentivirus surface does not enable infection of T cells. Viruses pseudotyped with VSVG-mut and SARS-CoV-2 S (wild type and prefusion-stabilized) infect <t>ACE2-expressing</t> <t>Jurkat</t> and CR3022 BCR-expressing <t>Ramos</t> cells and do not infect TCR-expressing Jurkat J76 cells (JM22 and 1G4 TCRs) or off-target VRC01-expressing Ramos cells. Positive controls confirm the functionality of pseudotyped viruses and cell lines: GL9/HLA-A*02:01 virus for JM22 J76 cells and NYESO/HLA-A*02:01 virus for 1G4 J76 cells. Data shown as mean ± SD for n = 3 technical replicates in addition to representative flow cytometry plots. P-values calculated with a one-way ANOVA with Tukey’s multiple comparisons correction. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.
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    Image Search Results


    KHK2840 is a novel human CD40 agonist. (A) Representative sensorgrams of hCD40 (left), cynomolgus monkey CD40 (middle left), rat CD40 (middle; right), and mouse CD40 (right) to KHK2840. KHK2840 was captured on the sensor chip immobilized with anti‐human IgG, and then dilutions of recombinant CD40 proteins (0.0625, 0.125, 0.250, 0.500, 1.00 μg/mL) were injected. (B) KHK2840 (red), isotype control (human anti‐DNP IgG2, gray), and positive control (human anti‐DNP IgG1, blue) bind to FcγRIIIa (left, 158F; middle, 158 V) and C1q. Data represent the mean ± SD of three experiments. (C) The in vitro expression of CD80, CD86, and IL‐12p70 in hmoDCs induced by KHK2840 (red) and isotype control (human anti‐DNP IgG2, gray). In IL‐12p70, values below the lower limit of quantification (LLOQ) were replaced with the LLOQ. Data represent the mean ± SD of three wells. The same experiments were repeated in a total of six donors. (D) The in vitro expression of CD86 and CD95 induced in Ramos cells by 0.01 (filled circle), 0.1 (blank circle), 1 (filled square) and 10 μg/mL (blank square) KHK2840 after 0.5, 4, 24, and 48 h of treatment. KHK2840 was washed out at the indicated time points. Cells were analyzed after a total of 48 h of incubation. Data represent the mean ± SD of three wells.

    Journal: Cancer Science

    Article Title: The novel and potent CD40 agonist KHK2840 augments the antitumor efficacy of anti‐ PD ‐1 antibody and paclitaxel

    doi: 10.1111/cas.16366

    Figure Lengend Snippet: KHK2840 is a novel human CD40 agonist. (A) Representative sensorgrams of hCD40 (left), cynomolgus monkey CD40 (middle left), rat CD40 (middle; right), and mouse CD40 (right) to KHK2840. KHK2840 was captured on the sensor chip immobilized with anti‐human IgG, and then dilutions of recombinant CD40 proteins (0.0625, 0.125, 0.250, 0.500, 1.00 μg/mL) were injected. (B) KHK2840 (red), isotype control (human anti‐DNP IgG2, gray), and positive control (human anti‐DNP IgG1, blue) bind to FcγRIIIa (left, 158F; middle, 158 V) and C1q. Data represent the mean ± SD of three experiments. (C) The in vitro expression of CD80, CD86, and IL‐12p70 in hmoDCs induced by KHK2840 (red) and isotype control (human anti‐DNP IgG2, gray). In IL‐12p70, values below the lower limit of quantification (LLOQ) were replaced with the LLOQ. Data represent the mean ± SD of three wells. The same experiments were repeated in a total of six donors. (D) The in vitro expression of CD86 and CD95 induced in Ramos cells by 0.01 (filled circle), 0.1 (blank circle), 1 (filled square) and 10 μg/mL (blank square) KHK2840 after 0.5, 4, 24, and 48 h of treatment. KHK2840 was washed out at the indicated time points. Cells were analyzed after a total of 48 h of incubation. Data represent the mean ± SD of three wells.

    Article Snippet: Human Burkitt's lymphoma Ramos (RA 1) cells (ATCC) were cultured in RPMI1640 supplemented with 10% FBS.

    Techniques: Recombinant, Injection, Control, Positive Control, In Vitro, Expressing, Incubation

    Fig. 2. SARS-CoV-2 displayed on lentivirus surface does not enable infection of T cells. Viruses pseudotyped with VSVG-mut and SARS-CoV-2 S (wild type and prefusion-stabilized) infect ACE2-expressing Jurkat and CR3022 BCR-expressing Ramos cells and do not infect TCR-expressing Jurkat J76 cells (JM22 and 1G4 TCRs) or off-target VRC01-expressing Ramos cells. Positive controls confirm the functionality of pseudotyped viruses and cell lines: GL9/HLA-A*02:01 virus for JM22 J76 cells and NYESO/HLA-A*02:01 virus for 1G4 J76 cells. Data shown as mean ± SD for n = 3 technical replicates in addition to representative flow cytometry plots. P-values calculated with a one-way ANOVA with Tukey’s multiple comparisons correction. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: SARS-CoV-2 spike does not interact with the T cell receptor or directly activate T cells.

    doi: 10.1073/pnas.2406615121

    Figure Lengend Snippet: Fig. 2. SARS-CoV-2 displayed on lentivirus surface does not enable infection of T cells. Viruses pseudotyped with VSVG-mut and SARS-CoV-2 S (wild type and prefusion-stabilized) infect ACE2-expressing Jurkat and CR3022 BCR-expressing Ramos cells and do not infect TCR-expressing Jurkat J76 cells (JM22 and 1G4 TCRs) or off-target VRC01-expressing Ramos cells. Positive controls confirm the functionality of pseudotyped viruses and cell lines: GL9/HLA-A*02:01 virus for JM22 J76 cells and NYESO/HLA-A*02:01 virus for 1G4 J76 cells. Data shown as mean ± SD for n = 3 technical replicates in addition to representative flow cytometry plots. P-values calculated with a one-way ANOVA with Tukey’s multiple comparisons correction. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.

    Article Snippet: Jurkat (ATCC TIB- 152), J76 cells, and Ramos cells (ATCC CRL- 1596) were cultured in RPMI- 1640 (ATCC) supplemented with 10% FBS and penicillin- streptomycin.

    Techniques: Infection, Expressing, Virus, Flow Cytometry