rabbit polyclonal anti glua2 (Millipore)

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Millipore rabbit polyclonal anti glua2

(A–D) <t>GLUA2</t> protein level is decreased at intracortical synapses in Gpc5 cKO mice at P28. (A) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. (B–D) Numbers of VGLUT1 (B), GLUA2 (C), and colocalized (D) puncta. N = 5 mice/condition. (E–H) GLUA1 protein level is unchanged at intracortical synapses in Gpc5 cKO mice at P28. (E) Immunostaining of presynaptic VGLUT1 and postsynaptic GLUA1 in L2/3. (F–H) Numbers of VGLUT1 (F), GLUA1 (G), and colocalized (H) puncta. N = 6 mice/condition. (I–P) Thalamocortical synapses have unaltered AMPAR levels in Gpc5 cKO mice at P28. (I) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA2 in L4. (J–L) Numbers of VGLUT2 (J), GLUA2 (K), and colocalized (L) puncta. N = 5 mice/condition. (M) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA1 in L4. (N–P) Numbers of VGLUT2 (N), GLUA1 (O), and colocalized (P) puncta. N = 5 mice/condition. (Q–S) VGLUT2 puncta volume is decreased in P28 Gpc5 cKO mice. (Q) Immunostaining of VGLUT2 puncta in L1 VC. (R and S) VGLUT2 puncta volume in L1 and L4. N = 6 mice/condition L1, N = 5 mice/condition L4. (T) Summary of synaptic changes in Gpc5 cKO mice at P28. Scale bars: 5 μm (A, E, I, M, and Q, main) and 1 μm (A, E, I, and M, magnified). Graphs: mean ± SEM. Individual data points represent mice. Statistics: t test. See also .

Rabbit Polyclonal Anti Glua2, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti-glua2/glua2+antibody/pmc12013928-3-0-5
Average 90 stars, based on 1 article reviews

rabbit polyclonal anti glua2 - by Bioz Stars, 2026-10

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1) Product Images from "Astrocyte glypican 5 regulates synapse maturation and stabilization"

Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization

Journal: Cell reports

doi: 10.1016/j.celrep.2025.115374

(A–D) GLUA2 protein level is decreased at intracortical synapses in Gpc5 cKO mice at P28. (A) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. (B–D) Numbers of VGLUT1 (B), GLUA2 (C), and colocalized (D) puncta. N = 5 mice/condition. (E–H) GLUA1 protein level is unchanged at intracortical synapses in Gpc5 cKO mice at P28. (E) Immunostaining of presynaptic VGLUT1 and postsynaptic GLUA1 in L2/3. (F–H) Numbers of VGLUT1 (F), GLUA1 (G), and colocalized (H) puncta. N = 6 mice/condition. (I–P) Thalamocortical synapses have unaltered AMPAR levels in Gpc5 cKO mice at P28. (I) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA2 in L4. (J–L) Numbers of VGLUT2 (J), GLUA2 (K), and colocalized (L) puncta. N = 5 mice/condition. (M) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA1 in L4. (N–P) Numbers of VGLUT2 (N), GLUA1 (O), and colocalized (P) puncta. N = 5 mice/condition. (Q–S) VGLUT2 puncta volume is decreased in P28 Gpc5 cKO mice. (Q) Immunostaining of VGLUT2 puncta in L1 VC. (R and S) VGLUT2 puncta volume in L1 and L4. N = 6 mice/condition L1, N = 5 mice/condition L4. (T) Summary of synaptic changes in Gpc5 cKO mice at P28. Scale bars: 5 μm (A, E, I, M, and Q, main) and 1 μm (A, E, I, and M, magnified). Graphs: mean ± SEM. Individual data points represent mice. Statistics: t test. See also .

Figure Legend Snippet: (A–D) GLUA2 protein level is decreased at intracortical synapses in Gpc5 cKO mice at P28. (A) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. (B–D) Numbers of VGLUT1 (B), GLUA2 (C), and colocalized (D) puncta. N = 5 mice/condition. (E–H) GLUA1 protein level is unchanged at intracortical synapses in Gpc5 cKO mice at P28. (E) Immunostaining of presynaptic VGLUT1 and postsynaptic GLUA1 in L2/3. (F–H) Numbers of VGLUT1 (F), GLUA1 (G), and colocalized (H) puncta. N = 6 mice/condition. (I–P) Thalamocortical synapses have unaltered AMPAR levels in Gpc5 cKO mice at P28. (I) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA2 in L4. (J–L) Numbers of VGLUT2 (J), GLUA2 (K), and colocalized (L) puncta. N = 5 mice/condition. (M) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA1 in L4. (N–P) Numbers of VGLUT2 (N), GLUA1 (O), and colocalized (P) puncta. N = 5 mice/condition. (Q–S) VGLUT2 puncta volume is decreased in P28 Gpc5 cKO mice. (Q) Immunostaining of VGLUT2 puncta in L1 VC. (R and S) VGLUT2 puncta volume in L1 and L4. N = 6 mice/condition L1, N = 5 mice/condition L4. (T) Summary of synaptic changes in Gpc5 cKO mice at P28. Scale bars: 5 μm (A, E, I, M, and Q, main) and 1 μm (A, E, I, and M, magnified). Graphs: mean ± SEM. Individual data points represent mice. Statistics: t test. See also .

Techniques Used: Immunostaining

(A–D) Overexpressing GPC5 in astrocytes in Gpc5 cKO VC is sufficient to increase the GLUA2 level. (A) Experimental design. (B) Membrane-GFP or HA-GPC5 colocalize with the astrocyte marker S100beta. Scale bar: 50 μm. (C) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. Scale bars: 5 μm (main) and 1 μm (magnified). (D) Number of GLUA2 puncta. N = 5–6 mice/condition. Graph: mean ± SEM. Data points represent mice. Statistics: t test. (E–G) Absence of GPC5 during the critical period does not impact large-scale remodeling in response to monocular enucleation (ME). (E) Experiment overview (F) Arc mRNA in the VC ipsilateral to the nondeprived eye 12 hours or 5 days after ME. Scale bar: 500 μm. (G) Width of the binocular zone. Graph: mean ± SEM. Individual points represent mice. N = 5 mice/condition. Statistics: two-way ANOVA. See also and .

Figure Legend Snippet: (A–D) Overexpressing GPC5 in astrocytes in Gpc5 cKO VC is sufficient to increase the GLUA2 level. (A) Experimental design. (B) Membrane-GFP or HA-GPC5 colocalize with the astrocyte marker S100beta. Scale bar: 50 μm. (C) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. Scale bars: 5 μm (main) and 1 μm (magnified). (D) Number of GLUA2 puncta. N = 5–6 mice/condition. Graph: mean ± SEM. Data points represent mice. Statistics: t test. (E–G) Absence of GPC5 during the critical period does not impact large-scale remodeling in response to monocular enucleation (ME). (E) Experiment overview (F) Arc mRNA in the VC ipsilateral to the nondeprived eye 12 hours or 5 days after ME. Scale bar: 500 μm. (G) Width of the binocular zone. Graph: mean ± SEM. Individual points represent mice. N = 5 mice/condition. Statistics: two-way ANOVA. See also and .

Techniques Used: Membrane, Marker, Immunostaining

(A–H) GLUA2 puncta numbers are recovered at intracortical synapses in Gpc5 cKO mice at P120. (A and E) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L1 (A) and L2/3 (E). (B–D and F–H) VGLUT1 (B and F), GLUA2 (C and G), and colocalized (D and H) puncta in L1 and L2/3. (I–K) VGLUT2 puncta volume is recovered at P120 in Gpc5 cKO mice. (I) Immunostaining of VGLUT2 in layer 1 VC. (J and K) VGLUT2 puncta volume in L1 (J) and L4 (K). N = 5 mice/condition. Scale bars: 5 μm (A, E, and I, main) and 1 μm (A and E, magnified). Graphs: mean ± SEM. Individual points represent mice. Statistics: t test. (L) Summary of synapse findings in Gpc5 cKO mice at P120. (M and N) Absence of astrocyte GPC5 enables enhanced ocular dominance plasticity in adulthood. (M) Arc mRNA in the VC ipsilateral to the nondeprived eye 12 hours or 5 days after ME. Scale bar: 500 μm. (N) Width of the binocular zone. Graph: mean ± SEM. Individual points represent mice. N = 5 mice/condition. Statistics: two-way ANOVA. (O) Summary of phenotypes in Gpc5 astrocyte-specific cKO mice. See also .

Figure Legend Snippet: (A–H) GLUA2 puncta numbers are recovered at intracortical synapses in Gpc5 cKO mice at P120. (A and E) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L1 (A) and L2/3 (E). (B–D and F–H) VGLUT1 (B and F), GLUA2 (C and G), and colocalized (D and H) puncta in L1 and L2/3. (I–K) VGLUT2 puncta volume is recovered at P120 in Gpc5 cKO mice. (I) Immunostaining of VGLUT2 in layer 1 VC. (J and K) VGLUT2 puncta volume in L1 (J) and L4 (K). N = 5 mice/condition. Scale bars: 5 μm (A, E, and I, main) and 1 μm (A and E, magnified). Graphs: mean ± SEM. Individual points represent mice. Statistics: t test. (L) Summary of synapse findings in Gpc5 cKO mice at P120. (M and N) Absence of astrocyte GPC5 enables enhanced ocular dominance plasticity in adulthood. (M) Arc mRNA in the VC ipsilateral to the nondeprived eye 12 hours or 5 days after ME. Scale bar: 500 μm. (N) Width of the binocular zone. Graph: mean ± SEM. Individual points represent mice. N = 5 mice/condition. Statistics: two-way ANOVA. (O) Summary of phenotypes in Gpc5 astrocyte-specific cKO mice. See also .

Techniques Used: Immunostaining

Figure Legend Snippet: KEY RESOURCES TABLE

Techniques Used: Recombinant, Modification, Saline, RNAscope, Multiplex Assay, Electron Microscopy, Negative Control, Software, Cell Culture, Fluorescence, Microscopy, Hybridization

Recombinant:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Tissue sections were incubated with primary antibodies: rabbit monoclonal anti-GluA1 (1:200; Millipore; Cat# 04-855; RRID: AB_1977216), rabbit polyclonal anti-GluA2 (1:200; Millipore; Cat# AB1768-25UG; RRID: AB_2247874), and mouse monoclonal anti-Synaptophysin (1:200; Millipore; Cat# MAB5258-50UG; RRID: AB_95187) overnight at room temperature and then incubated with fluorescent secondary antibody: goat anti-rabbit IgG Alexa Fluor 488 (1:100; Thermo Fisher Scientific; Cat# A-11034; RRID: AB_2576217) and goat anti-mouse IgG Alexa Fluor 647 (1:100; Thermo Fisher Scientific; Cat# A-21236; RRID: AB_2535805), followed by coverslipped with mounting medium containing DAPI.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly.96,97 Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04- 823, RRID: AB_1977218), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901) in blocking buffer.

Article Title: Inactivation of NMDA Receptors in the Ventral Tegmental Area during Cocaine Self-Administration Prevents GluA1 Upregulation but with Paradoxical Increases in Cocaine-Seeking Behavior
Article Snippet: Ten micrograms of protein aliquots of either membrane of cytosol fractions were separated by SDS-PAGE and transferred to a PVDF membrane and probed with rabbit polyclonal anti-GluA1 (1:500; Millipore, catalog #07-660, RRID: AB_390157 ), rabbit polycolonal anti-GluA2 (1:2000; Millipore, catalog #AB1768, RRID: AB_2313802 ), rabbit polyclonal anti-GluN2B (1:500; Millipore, catalog #06-600, RRID: AB_310193 ), rabbit polyclonal anti-GluN3A (1:500; Millipore, catalog #07-356, RRID: AB_2112620 ), and mouse monoclonal anti-pan cadherin (1:2000; Sigma-Aldrich, catalog #C1821, RRID: AB_476826 ) as an internal standard for membrane fractions, or mouse monoclonal anti-β-tubulin (1:100,000; Millipore, catalog #05-661, RRID: AB_309885 ), as an internal standard for cytosol fractions.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Article Title: Absence of chordin-like 1 aids motor recovery in a mouse model of stroke
Article Snippet: Sections were then incubated with the corresponding primary antibodies: rabbit polyclonal anti-GluA2 (dilution 1:1000, Millipore AB1768-I, RRID: AB_2922404), rabbit polyclonal anti-GluA1 (dilution 1:400, Millipore AB1504, RRID:AB_2113602) or rabbit polyclonal anti-PSD95 (dilution 1:400, Invitrogen 51-6900) in combination with guinea pig polyclonal anti-VGlut1 (dilution 1:2000, Millipore AB5905, RRID:AB_2301751) in 5% goat serum, 100 mM lysine in PBS and left overnight at 4 °C in a humidified chamber.

Article Title: Absence of chordin-like 1 aids motor recovery in a mouse model of stroke.
Article Snippet: Sections were then incubated with the corresponding primary antibodies: rabbit polyclonal anti-GluA2 (dilution 1:1000, Millipore AB1768-I, RRID: AB_2922404), rabbit polyclonal anti-GluA1 (dilution 1:400, Millipore AB1504, RRID:AB_2113602) or rabbit polyclonal anti-PSD95 (dilution 1:400, Invitrogen 51–6900) in combination with guinea pig polyclonal anti-VGlut1 (dilution 1:2000, Millipore AB5905, RRID:AB_2301751) in 5% goat serum, 100 mM lysine in PBS and left overnight at 4 ◦C in a humidified chamber.

Article Title: Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome
Article Snippet: Rabbit polyclonal anti-GluA2 , MilliPORE SiGMA , Cat#AB10529; RRID: AB_10807819.

Protease Inhibitor:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Blocking Assay:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Plasmid Preparation:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Enzyme-linked Immunosorbent Assay:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Bicinchoninic Acid Protein Assay:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Software:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Imaging:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Inverted Microscopy:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Microscopy:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Polyacrylamide Gel Electrophoresis:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Membrane:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Control:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Incubation:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Virus:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Mass Spectrometry:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

SDS Page:

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Rabbit Polyclonal Anti-GluA2 , Millipore , AB1768-25UG; RRID: AB_2247874.

Article Title: Disuse plasticity limits spinal cord injury recovery
Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Review

rabbit polyclonal anti glua2 (Millipore)

Structured Review

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1) Product Images from "Astrocyte glypican 5 regulates synapse maturation and stabilization"

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Recombinant:

Protease Inhibitor:

Blocking Assay:

Plasmid Preparation:

Enzyme-linked Immunosorbent Assay:

Bicinchoninic Acid Protein Assay:

Software:

Imaging:

Inverted Microscopy:

Microscopy:

Polyacrylamide Gel Electrophoresis:

Membrane:

Control:

Incubation:

Virus:

Mass Spectrometry:

SDS Page:

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