Blocking Assay:Article Title: Preserving Mafa Expression in Diabetic Islet β-Cells Improves Glycemic Control in Vivo
Article Snippet: .. After blocking with 3% donkey serum, immunolabeling was performed with the following antibodies: rabbit Mafa, 1:500 (Bethyl Laboratories, Inc., Montgomery, TX); rabbit myc tag, 1:200 (Cell Signaling Technology, Inc., Danvers, MA); guinea pig insulin, 1:1000 (DAKO, Glostrup, Denmark); mouse Ki-67, 1:500 (BD Biosciences); rabbit cleaved caspase-3, 1:500 (Cell Signaling Technology, Inc., Danvers, MA); mouse 4–4-hydroxy-2-nonenal, 1:200 (Japan Institute for the Control of Aging, Shizuoka, Japan); and rabbit GSTA1 (Thermo Fisher Scientific, Rockford, IL). .. The secondary antibodies used for fluorescent imaging on an Olympus FV1000-D confocal microscope were 488- or 555-conjugated donkey anti-guinea pig and anti-rabbit (Jackson ImmunoResearch Laboratories, 1:200).
Article Title: Preserving Mafa Expression in Diabetic Islet β-Cells Improves Glycemic Control in Vivo
Article Snippet: .. After blocking with 3% donkey serum, immunolabeling was performed with the following antibodies: rabbit Mafa, 1:500 (Bethyl Laboratories, Inc., Montgomery, TX); rabbit myc tag, 1:200 (Cell Signaling Technology, Inc., Danvers, MA); guinea pig insulin, 1:1000 (DAKO, Glostrup, Denmark); mouse Ki-67, 1:500 (BD Biosciences); rabbit cleaved caspase-3, 1:500 (Cell Signaling Technology, Inc., Danvers, MA); mouse 4 – 4-hydroxy-2-nonenal, 1:200 (Japan Institute for the Control of Aging, Shizuoka, Japan); and rabbit GSTA1 (Thermo Fisher Scientific, Rockford, IL). .. The secondary antibodies used for fluorescent imaging on an Olympus FV1000-D confocal microscope were 488- or 555-conjugated donkey anti-guinea pig and anti-rabbit (Jackson ImmunoResearch Laboratories, 1:200).
Immunolabeling:Article Title: Preserving Mafa Expression in Diabetic Islet β-Cells Improves Glycemic Control in Vivo
Article Snippet: .. After blocking with 3% donkey serum, immunolabeling was performed with the following antibodies: rabbit Mafa, 1:500 (Bethyl Laboratories, Inc., Montgomery, TX); rabbit myc tag, 1:200 (Cell Signaling Technology, Inc., Danvers, MA); guinea pig insulin, 1:1000 (DAKO, Glostrup, Denmark); mouse Ki-67, 1:500 (BD Biosciences); rabbit cleaved caspase-3, 1:500 (Cell Signaling Technology, Inc., Danvers, MA); mouse 4–4-hydroxy-2-nonenal, 1:200 (Japan Institute for the Control of Aging, Shizuoka, Japan); and rabbit GSTA1 (Thermo Fisher Scientific, Rockford, IL). .. The secondary antibodies used for fluorescent imaging on an Olympus FV1000-D confocal microscope were 488- or 555-conjugated donkey anti-guinea pig and anti-rabbit (Jackson ImmunoResearch Laboratories, 1:200).
Article Title: Preserving Mafa Expression in Diabetic Islet β-Cells Improves Glycemic Control in Vivo
Article Snippet: .. After blocking with 3% donkey serum, immunolabeling was performed with the following antibodies: rabbit Mafa, 1:500 (Bethyl Laboratories, Inc., Montgomery, TX); rabbit myc tag, 1:200 (Cell Signaling Technology, Inc., Danvers, MA); guinea pig insulin, 1:1000 (DAKO, Glostrup, Denmark); mouse Ki-67, 1:500 (BD Biosciences); rabbit cleaved caspase-3, 1:500 (Cell Signaling Technology, Inc., Danvers, MA); mouse 4 – 4-hydroxy-2-nonenal, 1:200 (Japan Institute for the Control of Aging, Shizuoka, Japan); and rabbit GSTA1 (Thermo Fisher Scientific, Rockford, IL). .. The secondary antibodies used for fluorescent imaging on an Olympus FV1000-D confocal microscope were 488- or 555-conjugated donkey anti-guinea pig and anti-rabbit (Jackson ImmunoResearch Laboratories, 1:200).
Control:Article Title: Preserving Mafa Expression in Diabetic Islet β-Cells Improves Glycemic Control in Vivo
Article Snippet: .. After blocking with 3% donkey serum, immunolabeling was performed with the following antibodies: rabbit Mafa, 1:500 (Bethyl Laboratories, Inc., Montgomery, TX); rabbit myc tag, 1:200 (Cell Signaling Technology, Inc., Danvers, MA); guinea pig insulin, 1:1000 (DAKO, Glostrup, Denmark); mouse Ki-67, 1:500 (BD Biosciences); rabbit cleaved caspase-3, 1:500 (Cell Signaling Technology, Inc., Danvers, MA); mouse 4–4-hydroxy-2-nonenal, 1:200 (Japan Institute for the Control of Aging, Shizuoka, Japan); and rabbit GSTA1 (Thermo Fisher Scientific, Rockford, IL). .. The secondary antibodies used for fluorescent imaging on an Olympus FV1000-D confocal microscope were 488- or 555-conjugated donkey anti-guinea pig and anti-rabbit (Jackson ImmunoResearch Laboratories, 1:200).
Article Title: Preserving Mafa Expression in Diabetic Islet β-Cells Improves Glycemic Control in Vivo
Article Snippet: .. After blocking with 3% donkey serum, immunolabeling was performed with the following antibodies: rabbit Mafa, 1:500 (Bethyl Laboratories, Inc., Montgomery, TX); rabbit myc tag, 1:200 (Cell Signaling Technology, Inc., Danvers, MA); guinea pig insulin, 1:1000 (DAKO, Glostrup, Denmark); mouse Ki-67, 1:500 (BD Biosciences); rabbit cleaved caspase-3, 1:500 (Cell Signaling Technology, Inc., Danvers, MA); mouse 4 – 4-hydroxy-2-nonenal, 1:200 (Japan Institute for the Control of Aging, Shizuoka, Japan); and rabbit GSTA1 (Thermo Fisher Scientific, Rockford, IL). .. The secondary antibodies used for fluorescent imaging on an Olympus FV1000-D confocal microscope were 488- or 555-conjugated donkey anti-guinea pig and anti-rabbit (Jackson ImmunoResearch Laboratories, 1:200).
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