rabbit derived ab (Bio-Rad)
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Rabbit Derived Ab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 347 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+derived+ab/Rabbit+anti+Protein+A/pmc12846922-64-30-33
Average 95 stars, based on 347 article reviews
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Incubation:Article Title: EZH2 PROTACs outperform catalytic inhibitors in prostate cancer by targeting a methylation-independent function of PRC2 Article Snippet: For coimmunoprecipitation, C4-2B were treated with DMSO or PROTAC-6272 (0.5uM) for 3 h, and cell pellets were lysed in Co-IP buffer (50 mM tris-HCl, pH 7.4 from Life Technology, 150 mM NaCl from VWR, 1 mM EDTA from Life Technology, 1% Triton X-100 from Sigma-Aldrich) supplemented with protease inhibitor (Roche). .. Whole lysates were incubated with primary antibodies overnight at 4 °C with agitation, followed by 1 h incubation with protein G-conjugated magnetic beads for mouse-derived Ab and protein A-conjugated for Article Title: Chemokine receptor CXCR7 activates AURKA and promotes neuroendocrine prostate cancer growth Article Snippet: For coimmunoprecipitation, HEK293T or C4-2B transiently transfected with the plasmids for 48 hours or C4-2B EnzR cells were lysed in Co-IP buffer (50mM tris-HCl pH 7.4 [Life Technology], 150mM NaCl [VWR], 1mM EDTA [Life Technology], 1% triton X-100 [Sigma-Aldrich]) supplemented with protease inhibitor (Roche). .. Whole lysates were incubated with primary antibodies overnight at 4°C with agitation, followed by 1 hour incubation with protein G-conjugated magnetic beads for mouse-derived Ab and protein A-conjugated for Article Title: Chemokine receptor CXCR7 activates Aurora Kinase A and promotes neuroendocrine prostate cancer growth Article Snippet: For coimmunoprecipitation, HEK293T or C4-2B transiently transfected with the plasmids for 48 hours or C4-2B EnzR cells were lysed in Co-IP buffer (50mM tris-HCl pH 7.4 [Life Technology], 150mM NaCl [VWR], 1mM EDTA [Life Technology], 1% triton X-100 [Sigma-Aldrich]) supplemented with protease inhibitor (Roche). .. Whole lysates were incubated with primary antibodies overnight at 4°C with agitation, followed by 1 hour incubation with protein G-conjugated magnetic beads for mouse-derived Ab and protein A-conjugated for Magnetic Beads:Article Title: EZH2 PROTACs outperform catalytic inhibitors in prostate cancer by targeting a methylation-independent function of PRC2 Article Snippet: For coimmunoprecipitation, C4-2B were treated with DMSO or PROTAC-6272 (0.5uM) for 3 h, and cell pellets were lysed in Co-IP buffer (50 mM tris-HCl, pH 7.4 from Life Technology, 150 mM NaCl from VWR, 1 mM EDTA from Life Technology, 1% Triton X-100 from Sigma-Aldrich) supplemented with protease inhibitor (Roche). .. Whole lysates were incubated with primary antibodies overnight at 4 °C with agitation, followed by 1 h incubation with protein G-conjugated magnetic beads for mouse-derived Ab and protein A-conjugated for Article Title: Chemokine receptor CXCR7 activates AURKA and promotes neuroendocrine prostate cancer growth Article Snippet: For coimmunoprecipitation, HEK293T or C4-2B transiently transfected with the plasmids for 48 hours or C4-2B EnzR cells were lysed in Co-IP buffer (50mM tris-HCl pH 7.4 [Life Technology], 150mM NaCl [VWR], 1mM EDTA [Life Technology], 1% triton X-100 [Sigma-Aldrich]) supplemented with protease inhibitor (Roche). .. Whole lysates were incubated with primary antibodies overnight at 4°C with agitation, followed by 1 hour incubation with protein G-conjugated magnetic beads for mouse-derived Ab and protein A-conjugated for Article Title: Chemokine receptor CXCR7 activates Aurora Kinase A and promotes neuroendocrine prostate cancer growth Article Snippet: For coimmunoprecipitation, HEK293T or C4-2B transiently transfected with the plasmids for 48 hours or C4-2B EnzR cells were lysed in Co-IP buffer (50mM tris-HCl pH 7.4 [Life Technology], 150mM NaCl [VWR], 1mM EDTA [Life Technology], 1% triton X-100 [Sigma-Aldrich]) supplemented with protease inhibitor (Roche). .. Whole lysates were incubated with primary antibodies overnight at 4°C with agitation, followed by 1 hour incubation with protein G-conjugated magnetic beads for mouse-derived Ab and protein A-conjugated for |


