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quickchange kit  (Qiagen)


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    Structured Review

    Qiagen quickchange kit
    Quickchange Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quickchange/quickchange+kit/pmc12205366-60-26-28
    Average 90 stars, based on 1 article reviews
    quickchange kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Tyrosine kinase inhibitors
    Article Snippet: Site-directed mutagenesis (QuickChange, Qiagen) was performed on each construct at a single nucleotide whereby upon translation C1156→Y, or F1174→L, or L1196→M, or R1275→Q.

    Binding Assay:

    Article Title: Regulators of aldehyde dehydrogenase ALDH3A1 and related therapeutic methods
    Article Snippet: .. In order to characterize the binding pattern of CB7 to catalytic site of ALDH3A1, two important mutations Q122A and Q122W were made. oint mutations of ALDH3A1 were performed using QuickChange (Qiagen) mutagenesis. .. In order to characterize the binding pattern of CB7 to catalytic site of ALDH3A1, two important mutations Q122A and Q122W were made. oint mutations of ALDH3A1 were performed using QuickChange (Qiagen) mutagenesis.

    Mutagenesis:

    Article Title: Regulators of aldehyde dehydrogenase ALDH3A1 and related therapeutic methods
    Article Snippet: .. In order to characterize the binding pattern of CB7 to catalytic site of ALDH3A1, two important mutations Q122A and Q122W were made. oint mutations of ALDH3A1 were performed using QuickChange (Qiagen) mutagenesis. .. In order to characterize the binding pattern of CB7 to catalytic site of ALDH3A1, two important mutations Q122A and Q122W were made. oint mutations of ALDH3A1 were performed using QuickChange (Qiagen) mutagenesis.

    Article Title: Selective ALDH3A1 Inhibition by Benzimidazole Analogues Increase Mafosfamide Sensitivity in Cancer Cells
    Article Snippet: .. Point mutations of ALDH3A1 were performed using QuickChange (Qiagen) mutagenesis. ..

    Article Title: Regulator of G protein signaling 2 (RGS2) and RGS4 form distinct G protein-dependent complexes with protease activated-receptor 1 (PAR1) in live cells.
    Article Snippet: .. To generate a Gq/11-insensitive mutant of PAR1 (R205A) [24] for BRET studies, the wild type mPAR1-Ven plasmid (above) was used as a template for amplification by QuickChange (Qiagen) using primers, 59-CAT AAG CAT TGA CGC GTT CCT GGC GGT G-39 (forward) and 59-CAC CGC CAG GAA CGC GTC AAT GCT TAT G- 39(reverse). ..

    Article Title: Selective ALDH3A1 Inhibition by Benzimidazole Analogues Increase Mafosfamide Sensitivity in Cancer Cells
    Article Snippet: .. Point mutations of ALDH3A1 were performed using QuickChange (Qiagen) mutagenesis. ..

    Article Title: Regulator of G Protein Signaling 2 (RGS2) and RGS4 Form Distinct G Protein-Dependent Complexes with Protease Activated-Receptor 1 (PAR1) in Live Cells
    Article Snippet: .. To generate a G q/11 -insensitive mutant of PAR1 (R205A) for BRET studies, the wild type mPAR1-Ven plasmid (above) was used as a template for amplification by QuickChange (Qiagen) using primers, 5′-CAT AAG CAT TGA CGC GTT CCT GGC GGT G-3′ (forward) and 5′-CAC CGC CAG GAA CGC GTC AAT GCT TAT G- 3′(reverse). ..

    Bioluminescence Resonance Energy Transfer:

    Article Title: Regulator of G protein signaling 2 (RGS2) and RGS4 form distinct G protein-dependent complexes with protease activated-receptor 1 (PAR1) in live cells.
    Article Snippet: .. To generate a Gq/11-insensitive mutant of PAR1 (R205A) [24] for BRET studies, the wild type mPAR1-Ven plasmid (above) was used as a template for amplification by QuickChange (Qiagen) using primers, 59-CAT AAG CAT TGA CGC GTT CCT GGC GGT G-39 (forward) and 59-CAC CGC CAG GAA CGC GTC AAT GCT TAT G- 39(reverse). ..

    Article Title: Regulator of G Protein Signaling 2 (RGS2) and RGS4 Form Distinct G Protein-Dependent Complexes with Protease Activated-Receptor 1 (PAR1) in Live Cells
    Article Snippet: .. To generate a G q/11 -insensitive mutant of PAR1 (R205A) for BRET studies, the wild type mPAR1-Ven plasmid (above) was used as a template for amplification by QuickChange (Qiagen) using primers, 5′-CAT AAG CAT TGA CGC GTT CCT GGC GGT G-3′ (forward) and 5′-CAC CGC CAG GAA CGC GTC AAT GCT TAT G- 3′(reverse). ..

    Plasmid Preparation:

    Article Title: Regulator of G protein signaling 2 (RGS2) and RGS4 form distinct G protein-dependent complexes with protease activated-receptor 1 (PAR1) in live cells.
    Article Snippet: .. To generate a Gq/11-insensitive mutant of PAR1 (R205A) [24] for BRET studies, the wild type mPAR1-Ven plasmid (above) was used as a template for amplification by QuickChange (Qiagen) using primers, 59-CAT AAG CAT TGA CGC GTT CCT GGC GGT G-39 (forward) and 59-CAC CGC CAG GAA CGC GTC AAT GCT TAT G- 39(reverse). ..

    Article Title: Regulator of G Protein Signaling 2 (RGS2) and RGS4 Form Distinct G Protein-Dependent Complexes with Protease Activated-Receptor 1 (PAR1) in Live Cells
    Article Snippet: .. To generate a G q/11 -insensitive mutant of PAR1 (R205A) for BRET studies, the wild type mPAR1-Ven plasmid (above) was used as a template for amplification by QuickChange (Qiagen) using primers, 5′-CAT AAG CAT TGA CGC GTT CCT GGC GGT G-3′ (forward) and 5′-CAC CGC CAG GAA CGC GTC AAT GCT TAT G- 3′(reverse). ..

    Amplification:

    Article Title: Regulator of G protein signaling 2 (RGS2) and RGS4 form distinct G protein-dependent complexes with protease activated-receptor 1 (PAR1) in live cells.
    Article Snippet: .. To generate a Gq/11-insensitive mutant of PAR1 (R205A) [24] for BRET studies, the wild type mPAR1-Ven plasmid (above) was used as a template for amplification by QuickChange (Qiagen) using primers, 59-CAT AAG CAT TGA CGC GTT CCT GGC GGT G-39 (forward) and 59-CAC CGC CAG GAA CGC GTC AAT GCT TAT G- 39(reverse). ..

    Article Title: Regulator of G Protein Signaling 2 (RGS2) and RGS4 Form Distinct G Protein-Dependent Complexes with Protease Activated-Receptor 1 (PAR1) in Live Cells
    Article Snippet: .. To generate a G q/11 -insensitive mutant of PAR1 (R205A) for BRET studies, the wild type mPAR1-Ven plasmid (above) was used as a template for amplification by QuickChange (Qiagen) using primers, 5′-CAT AAG CAT TGA CGC GTT CCT GGC GGT G-3′ (forward) and 5′-CAC CGC CAG GAA CGC GTC AAT GCT TAT G- 3′(reverse). ..

    Generated:

    Article Title: Hereditary spastic paraplegia SPG13 mutation increases structural stability and ATPase activity of human mitochondrial chaperonin.
    Article Snippet: .. To remove the C-terminal 26 a.a. Gly-Met repeats, primers (5’) AAA CAT ATG GCT AAA GAT GTG AAG TTT and (3’) TTT CTT AAG TTA TGG GTC CTT CTC T were used to generate mHsp60GMless. mHsp60 mutants, mHsp60F72I, mHsp60V72A and their truncated counterparts were generated using QuickChange (Qiagen). ..

    Article Title: Hereditary spastic paraplegia SPG13 mutation increases structural stability and ATPase activity of human mitochondrial chaperonin
    Article Snippet: .. To remove the C-terminal 26 a.a. Gly-Met repeats, primers (5’) AAACATATGGCTAAAGATGTGAAGTTT and (3’) TTTCTTAAGTTATGGGTCCTTCTCT were used to generate mHsp60 GMless . mHsp60 mutants, mHsp60 F72I , mHsp60 V72A and their truncated counterparts were generated using QuickChange (Qiagen). ..



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