Journal: Frontiers in Veterinary Science
Article Title: In vitro chemosensitivity of a canine tumor venereal transmissible cancer cell line
doi: 10.3389/fvets.2022.972185
Figure Lengend Snippet: Effect of different pharmacological agents on the viability of the CTVT cell line. CTVT cells (5x10 3 /well) were exposed to different concentrations (from 0 to 100 μg/ml) of the drugs Vin, Dox, Tmz, Pan, Gem, Toc, 5-Fu, Cyclo, Metho, and Cis for 24 h. Relative cell viability was determined by the Alamar-blue assay. (A) Each bar graph represents the average of 6 independent measurements ± SD. Statistical significance was performed using Dunnett's post hoc test ( * p < 0.05). (B) The CC 50 of each drug was calculated from the viability data obtained with the Alamar blue assay using the Quest GraphTM ED50 Calculator program (AAT Bioquest, Inc.). The CC 50 is expressed in μg/ml. (C) Representative photographs taken under a bright field microscopy of TVT cells treated with the CC 50 of each drug. Magnification: 40 ×. Vin, vincristine; Dox, doxorubicin; Tmz, temozolomide; Pan, panobinostat; Gem, gemcitabine; Toc, toceranib; 5-Fu, 5-fluorouracil; Cyclo, cyclophosphamide; Metho, methotrexate; Cis, cisplatin.
Article Snippet: The CC 50 of each drug was calculated from the viability data obtained with the Alamar blue assay using the Quest GraphTM ED50 Calculator program (AAT Bioquest, Inc.) ( , ).
Techniques: Alamar Blue Assay, Microscopy