equivalent miniprep plasmid purification kit  (Bio-Rad)

Journal: BioMed Research International

Article Title: Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics

doi: 10.1155/2017/9306564

Figure Lengend Snippet: Summary of advantages and disadvantages of the main NAE methods. GuSCN, guanidine thiocyanate; CsCl, cesium chloride; EtBr, ethidium bromide; CTAB, cetyltrimethylammonium bromide.

Article Snippet: Diatomaceous Earth , Quantum Prep Plasmid Purification Kits (e.g., Bio-Rad) , Culture bacteria (1-2 mL liquid culture) , Up to 40 μ g , [ ] .

Techniques: Extraction, Gradient Centrifugation, Chromatography, Purification

Journal: BioMed Research International

Article Title: Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics

doi: 10.1155/2017/9306564

Figure Lengend Snippet: Examples of commercially available kits applying each extraction method and typical yields for distinct samples.

Article Snippet: Diatomaceous Earth , Quantum Prep Plasmid Purification Kits (e.g., Bio-Rad) , Culture bacteria (1-2 mL liquid culture) , Up to 40 μ g , [ ] .

Techniques: Extraction, Plasmid Preparation, Cell Culture, Bacteria, DNA Purification, Purification

Journal:

Article Title: A Rapid Method for Manual or Automated Purification of Fluorescently Labeled Nucleic Acids for Sequencing, Genotyping, and Microarrays

doi:

Figure Lengend Snippet: Effects of unincorporated dye precursors on fluorescent nucleic acid analyses. A, B: Comparison of DNA sequencing analyses performed without (A) or with (B) purification of unincorporated dye terminators. Sequencing reactions were prepared from 250 ng pGEMZf(+) plasmid and 4 pmol M13 −47 primer, using BigDye version 3.0 at a 2:10 (μL BigDye:μL total volume) reaction mixture with Better Buffer diluent. Purification B was performed with the RapXtract II Kit. DNA sequences were analyzed on an ABI PRISM 3100 Genetic Analyzer using POP6 polymer and injection parameters recommended for RapXtract Kits (2 kV, 45 s). C, D: Comparison of cDNA-microarray hybridization analyses performed without (C) or with (D) purification of unincorporated dye-labeled nucleotides from the labeling reaction. Labeled cDNA was prepared from 1 μg Saccharomyces cerevisiae mRNA using the CyScribe First Strand Labeling Kit and Cy3-labeled dUTP. Purification D was performed with the RapXtract Fluorescent Nucleic Acid Purification Kit. The cDNA was then hybridized to a yeast collage array at 67°C for 12 h. The microarray was visualized on a ScanArray Lite scanner at a laser output of 80%, PMT gain of 80%, and spatial resolution of 50 μm.

Article Snippet: Templates were purified using the Plasmid Purification System (Qiagen, Valencia, CA) or the Quantum Prep Plasmid Purification Kit (Bio-Rad, Hercules, CA).

Techniques: Comparison, DNA Sequencing, Purification, Sequencing, Plasmid Preparation, Polymer, Injection, Microarray, Hybridization, Labeling, Nucleic Acid Purification

Journal:

Article Title: A Rapid Method for Manual or Automated Purification of Fluorescently Labeled Nucleic Acids for Sequencing, Genotyping, and Microarrays

doi:

Figure Lengend Snippet: Schematic illustrating RapXtract “reverse purification” with a DNA sequencing reaction.

Article Snippet: Templates were purified using the Plasmid Purification System (Qiagen, Valencia, CA) or the Quantum Prep Plasmid Purification Kit (Bio-Rad, Hercules, CA).

Techniques: Purification, DNA Sequencing

Journal:

Article Title: A Rapid Method for Manual or Automated Purification of Fluorescently Labeled Nucleic Acids for Sequencing, Genotyping, and Microarrays

doi:

Figure Lengend Snippet: Representative chromatograph of a DNA sequence prepared from BigDye version 3.0 and purified with RapXtract particles. The sequencing reaction was prepared from 250 ng pUC19 DNA and 4 pmol M13 −47 primer, using BigDye version 3.0 at a 4:10 (μL BigDye:μL total volume) reaction mixture. Automated purification was performed in 384-well microplates with the RapXtract 384 Kit, using a Tomtec Quadra 3. Sequencing reaction samples were analyzed on an ABI PRISM 3700 DNA Analyzer using POP5 polymer and injection parameters recommended for RapXtract Kits (2.5 kV, 45 s).

Article Snippet: Templates were purified using the Plasmid Purification System (Qiagen, Valencia, CA) or the Quantum Prep Plasmid Purification Kit (Bio-Rad, Hercules, CA).

Techniques: Sequencing, Purification, Polymer, Injection

Journal:

Article Title: A Rapid Method for Manual or Automated Purification of Fluorescently Labeled Nucleic Acids for Sequencing, Genotyping, and Microarrays

doi:

Figure Lengend Snippet: Bar graphs representing sequence quality (Q20) and signal strength obtained from DNA sequences purified with RapXtract particles. Sequencing reactions were prepared from 250 ng pUC19 DNA and 4 pmol M13 −47 primer, using BigDye version 1.0 at a dye dilution ratio of 1:10 (μL BigDye:μL total volume). Sixty-four reactions were prepared using 5× Sequencing Reagent as a diluent (A) and 64 were prepared using Better Buffer as a diluent (B). RapXtract purification was performed in 384-well microplates with the RapXtract 384 Kit, using a Tomtec Quadra 3. DNA sequences were analyzed on an ABI PRISM 3100 Genetic Analyzer using POP6 polymer and injection parameters recommended for RapXtract Kits (2 kV, 45 s). Sequence quality is shown as the Q20 score, assessed using the Phred basecaller software. Signal strengths were recorded by the Sequencing Autoscore version 2.0 software provided with the ABI PRISM 3100.

Article Snippet: Templates were purified using the Plasmid Purification System (Qiagen, Valencia, CA) or the Quantum Prep Plasmid Purification Kit (Bio-Rad, Hercules, CA).

Techniques: Sequencing, Purification, Polymer, Injection, Software

Journal:

Article Title: A Rapid Method for Manual or Automated Purification of Fluorescently Labeled Nucleic Acids for Sequencing, Genotyping, and Microarrays

doi:

Figure Lengend Snippet: Comparison showing relationships of sequence quality (Q20) and signal strength for two sets of templates. Sequencing reactions were BigDye version 1.0 at a dye dilution ratio of 3:10 (μL BigDye:μL total volume) (A) or 2:10 (μL BigDye:μL total volume) (B) using 5× Sequencing Buffer as a diluent. Samples were prepared from approximately 100 ng H. akashiwo chloroplast amplicon DNA (various templates) and 4 pmol M13 forward primer (A) or from 250 ng pUC19 DNA and 4 pmol M13 −47 forward primer (B). Purification was with the RapXtract II Kit, manual protocol. DNA sequences were analyzed on an ABI PRISM 3100 Genetic Analyzer using POP6 polymer and injection parameters recommended for RapXtract Kits (2 kV, 45 s) (A) or instrument default parameters (1.5 kV, 30 s) (B). Q20 scores were assessed using the Phred basecaller software. Signal strengths were recorded by the Sequencing Autoscore version 2.0 software provided with the ABI PRISM 3100.

Article Snippet: Templates were purified using the Plasmid Purification System (Qiagen, Valencia, CA) or the Quantum Prep Plasmid Purification Kit (Bio-Rad, Hercules, CA).

Techniques: Comparison, Sequencing, Amplification, Purification, Polymer, Injection, Software

Journal:

Article Title: A Rapid Method for Manual or Automated Purification of Fluorescently Labeled Nucleic Acids for Sequencing, Genotyping, and Microarrays

doi:

Figure Lengend Snippet: Representative trace of a DNA sequence prepared using IRDye700 and IRDye800 near-infrared dyes and purified with RapXtract particles. The sequencing reaction was prepared from 1.5 μg noncommercially prepared plasmid DNA (3–7 kb) and 3 pmol M13 forward primer, using IRDye700 Termination Mix at full strength in a 2:8.5 (μL IRDye:μL total volume) reaction mixture. Purification was with the RapXtract II Kit, manual protocol. Sequencing reaction samples were analyzed on an NEN Global IR2 DNA Analyzer.

Article Snippet: Templates were purified using the Plasmid Purification System (Qiagen, Valencia, CA) or the Quantum Prep Plasmid Purification Kit (Bio-Rad, Hercules, CA).

Techniques: Sequencing, Purification, Plasmid Preparation

Journal:

Article Title: A Rapid Method for Manual or Automated Purification of Fluorescently Labeled Nucleic Acids for Sequencing, Genotyping, and Microarrays

doi:

Figure Lengend Snippet: Comparison of Purification of DNA Sequencing Reactions by RapXtract II Kit or by Ethanol Precipitation Using IRDye700 and IRDye800 Terminators

Article Snippet: Templates were purified using the Plasmid Purification System (Qiagen, Valencia, CA) or the Quantum Prep Plasmid Purification Kit (Bio-Rad, Hercules, CA).

Techniques: Comparison, Purification, DNA Sequencing, Ethanol Precipitation

Journal:

Article Title: A Rapid Method for Manual or Automated Purification of Fluorescently Labeled Nucleic Acids for Sequencing, Genotyping, and Microarrays

doi:

Figure Lengend Snippet: Dual-color RNA-microarray hybridization analysis showing H. influenzae RNA from cells grown aerobically (Alexa Fluor dye 594, indicated by green) or anaerobically (Alexa Fluor dye 660, indicated by red). Purification of labeled RNA was performed with the RapXtract Fluorescent Nucleic Acid Purification Kit. The RNA was then hybridized to an H. influenzae gene microarray glass slide at 42°C for 15 h, and results were visualized in a ScanArray 5000 with laser output of 100% (Alexa Fluor 594) or 95% (Alexa Fluor 660), PMT gain of 70% (Alexa Fluor 594) or 63% (Alexa Fluor 660), and spatial resolution of 10 μm.

Article Snippet: Templates were purified using the Plasmid Purification System (Qiagen, Valencia, CA) or the Quantum Prep Plasmid Purification Kit (Bio-Rad, Hercules, CA).

Techniques: Microarray, Hybridization, Purification, Labeling, Nucleic Acid Purification

Journal:

Article Title: A Rapid Method for Manual or Automated Purification of Fluorescently Labeled Nucleic Acids for Sequencing, Genotyping, and Microarrays

doi:

Figure Lengend Snippet: Spectrophotometric analysis of different methods for purification of Cy5-labeled cDNA. Six reactions of the Cy5-labeled mouse cDNA were combined and then split into six equal parts for purification. Purification methods for samples shown are: (1) unpurified, (2) size exclusion type 1 (Auto-Seq G-50, Amersham Biosciences), (3) size exclusion type 2 (Micro Bio-Spin 6, Bio-Rad), (4) size exclusion type 3 (Microcon YM-30, Millipore), (5) size exclusion type 4 (Microcon YM-100, Millipore), and (6) RapXtract Fluorescent Nucleic Acid Purification Kit. All samples were diluted 1:10 in 10 mM Tris-HCl, 140 mM NaCl, pH 8, and were scanned in an HP 845x UV-Visible Chemstation. A: Spectral scans from each sample. B: List of absorbance values for each sample at 260 nm (absorbance maximum for cDNA) and 650 nm (absorbance maximum for Cy5 Dye).

Article Snippet: Templates were purified using the Plasmid Purification System (Qiagen, Valencia, CA) or the Quantum Prep Plasmid Purification Kit (Bio-Rad, Hercules, CA).

Techniques: Purification, Labeling, Nucleic Acid Purification