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quantikine elisa kit  (R&D Systems)


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    R&D Systems quantikine elisa kit
    Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantikine+elisa+kit/Human+M-CSF+Quantikine+ELISA+Kit/pm42821460-65-17-22
    Average 94 stars, based on 68 article reviews
    quantikine elisa kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Processes for production of tumor infiltrating lymphocytes and uses of same in immunotherapy
    Article Snippet: .. Production of IFN-γ was measured using Quantikine ELISA kit by R&D systems. ..

    Article Title: Ethanolic Extract of Padina arborescens Suppresses Melanogenesis and Attenuates UVB-Induced Photodamage in Cellular and Zebrafish Models
    Article Snippet: .. After 24 h of incubation, culture supernatants were collected, and human IL-1β levels were measured using the Quantikine® ELISA Kit (Human IL-1β/IL-1F2, Catalog #DLB50; R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. ..

    Article Title: From bats to humans: uncovering ISG15 as a new resistance factor in type 1 diabetes
    Article Snippet: .. Human CXCL10/IP-10 Immunoassay (Quantikine ELISA kit, R&D Systems) was used to quantify CXCL10 secretion to the culture medium as indicated in the figure legends. .. Human ISG15 ELISA Kit (Abcam) was used to quantify ISG15 secretion to the culture medium as indicated in the figure legends.

    Article Title: INTERLEUKIN-1 RECEPTOR ANTAGONIST LEVELS IN PATIENTS WITH HEART FAILURE AND REDUCED EJECTION FRACTION TREATED WITH ANAKINRA
    Article Snippet: .. Plasma samples were analyzed using enzyme-linked immunosorbent assay (Quantikine ELISA Kit, R&D Systems) to measure human interleukin-1Ra (Human IL-1Ra/IL-1F3) following the manufacturer’s instructions. ..

    Article Title: The role of S100A8/A9 in the pathogenesis of acute exacerbations of pulmonary fibrosis.
    Article Snippet: .. 140 DuoSet ELISA Kit (R&D Systems, Minneapolis, MN, USA) was used to detect S100A8/A9 141 levels (both human and mouse), whereas the Quantikine ELISA Kit (R&D Systems) was used 142 to evaluate the levels of other cytokines, according to the manufacturer’s protocols. ..

    Article Title: Plasma biomarker‐informed brain topology for cognitive resilience: applications in non‐pharmacological interventions concerning cognitive aging
    Article Snippet: .. For a proportion of ACT participants, we measured plasma p‐tau 217 using an ALZpath Simoa® p‐tau217 version 2 Assay Kit (Lot No. 999024, MAB031022) on an HD‐X analyzer (Quanterix, MA, USA), plasma GFAP, NfL, Aβ 1‐42 using the Quanterix Neurology 4 plex assay E (N4PE) kit (Lot No. 503940) on an HD‐X analyzer (Quanterix), and serum free‐BDNF using a Quantikine ELISA kit (Lot No. P386518) (R&D Systems, Minneapolis, MN, USA). .. All statistics were implemented with built‐in functions in IBM SPSS Statistics version 29.0.2.0, and all figures were plotted in the ggplot2 R package.

    Article Title: Neutrophils and CSF1 promote the expansion of monocyte-derived alveolar macrophages in progressive pulmonary fibrosis.
    Article Snippet: Neutrophils and CSF1 promote the expansion of monocyte-derived alveolar 1 macrophages in progressive pulmonary fibrosis 2 3 Kazumasa Kase1, Satoshi Watanabe1, Masafumi Horie2,3, Atsushi Muto1, Kazuhiko 4 Iwasaki1, Kyota Akasaki1, Takafumi Kobayashi1, Sachiko Arai1,7, Hayato Koba1, Kenta 5 Yamamura1, Shigeki Nanjo1, Yuichi Tambo1, Noriyuki Ohkura1, Miki Abo1, Tomohiro 6 Iba4, Hitoshi Ando4, Takashi Matsushita5, Hisamichi Naito6, Seiji Yano1,7 7 8 1Department of Respiratory Medicine, Kanazawa University Graduate School of Medical 9 Sciences, Kanazawa, Ishikawa, Japan 10 2Department of Molecular and Cellular Pathology, Kanazawa University Graduate School of 11 Medical Sciences, Kanazawa, Ishikawa, Japan 12 3Department of Molecular and Genomic Pathology, Kobe University Graduate School of 13 Medicine, Kobe, Hyogo, Japan 14 4Department of Cellular and Molecular Function Analysis, Kanazawa University Graduate 15 School of Medical Sciences, Kanazawa, Ishikawa, Japan 16 5Department of Dermatology, Kanazawa University Graduate School of Medical Sciences, 17 Kanazawa, Ishikawa, Japan 18 6Department of Vascular Physiology, Kanazawa University Graduate School of Medical 19 Sciences, Japan 20 7WPI Nano Life Science Institute, Kanazawa University, Kanazawa, Ishikawa, Japan 21 22 Running head: Neutrophil and CSF1 in progressive pulmonary fibrosis 23 24 Corresponding Author: 25 Satoshi Watanabe, MD, PhD 26 Department of Respiratory Medicine, 27 Kanazawa University Graduate School of Medical Sciences 28 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8641, Japan 29 Tel: +81-076-265-2146 30 Fax: +81-076-234-4215 31 E-mail: swatanabe@staff.kanazawa-u.ac.jp 32 33

    Incubation:

    Article Title: Ethanolic Extract of Padina arborescens Suppresses Melanogenesis and Attenuates UVB-Induced Photodamage in Cellular and Zebrafish Models
    Article Snippet: .. After 24 h of incubation, culture supernatants were collected, and human IL-1β levels were measured using the Quantikine® ELISA Kit (Human IL-1β/IL-1F2, Catalog #DLB50; R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. ..

    other:

    Article Title: Doxorubicin enhances adipogenesis in an FGF2-dependent manner and induces a tumour-promoting secretory phenotype
    Article Snippet: Coverslips were washed twice with PBS and then TUNEL reaction mixture (cat # 11684795910, In Situ Cell Death Detection Kit, Fluorescein, Roche, Mannheim, Germany) was added to the sample and incubated for 1 h at 37°C in the dark.

    Clinical Proteomics:

    Article Title: INTERLEUKIN-1 RECEPTOR ANTAGONIST LEVELS IN PATIENTS WITH HEART FAILURE AND REDUCED EJECTION FRACTION TREATED WITH ANAKINRA
    Article Snippet: .. Plasma samples were analyzed using enzyme-linked immunosorbent assay (Quantikine ELISA Kit, R&D Systems) to measure human interleukin-1Ra (Human IL-1Ra/IL-1F3) following the manufacturer’s instructions. ..

    Article Title: Plasma biomarker‐informed brain topology for cognitive resilience: applications in non‐pharmacological interventions concerning cognitive aging
    Article Snippet: .. For a proportion of ACT participants, we measured plasma p‐tau 217 using an ALZpath Simoa® p‐tau217 version 2 Assay Kit (Lot No. 999024, MAB031022) on an HD‐X analyzer (Quanterix, MA, USA), plasma GFAP, NfL, Aβ 1‐42 using the Quanterix Neurology 4 plex assay E (N4PE) kit (Lot No. 503940) on an HD‐X analyzer (Quanterix), and serum free‐BDNF using a Quantikine ELISA kit (Lot No. P386518) (R&D Systems, Minneapolis, MN, USA). .. All statistics were implemented with built‐in functions in IBM SPSS Statistics version 29.0.2.0, and all figures were plotted in the ggplot2 R package.

    Plex Assay:

    Article Title: Plasma biomarker‐informed brain topology for cognitive resilience: applications in non‐pharmacological interventions concerning cognitive aging
    Article Snippet: .. For a proportion of ACT participants, we measured plasma p‐tau 217 using an ALZpath Simoa® p‐tau217 version 2 Assay Kit (Lot No. 999024, MAB031022) on an HD‐X analyzer (Quanterix, MA, USA), plasma GFAP, NfL, Aβ 1‐42 using the Quanterix Neurology 4 plex assay E (N4PE) kit (Lot No. 503940) on an HD‐X analyzer (Quanterix), and serum free‐BDNF using a Quantikine ELISA kit (Lot No. P386518) (R&D Systems, Minneapolis, MN, USA). .. All statistics were implemented with built‐in functions in IBM SPSS Statistics version 29.0.2.0, and all figures were plotted in the ggplot2 R package.

    Concentration Assay:

    Article Title: Neutrophils and CSF1 promote the expansion of monocyte-derived alveolar macrophages in progressive pulmonary fibrosis.
    Article Snippet: Neutrophils and CSF1 promote the expansion of monocyte-derived alveolar 1 macrophages in progressive pulmonary fibrosis 2 3 Kazumasa Kase1, Satoshi Watanabe1, Masafumi Horie2,3, Atsushi Muto1, Kazuhiko 4 Iwasaki1, Kyota Akasaki1, Takafumi Kobayashi1, Sachiko Arai1,7, Hayato Koba1, Kenta 5 Yamamura1, Shigeki Nanjo1, Yuichi Tambo1, Noriyuki Ohkura1, Miki Abo1, Tomohiro 6 Iba4, Hitoshi Ando4, Takashi Matsushita5, Hisamichi Naito6, Seiji Yano1,7 7 8 1Department of Respiratory Medicine, Kanazawa University Graduate School of Medical 9 Sciences, Kanazawa, Ishikawa, Japan 10 2Department of Molecular and Cellular Pathology, Kanazawa University Graduate School of 11 Medical Sciences, Kanazawa, Ishikawa, Japan 12 3Department of Molecular and Genomic Pathology, Kobe University Graduate School of 13 Medicine, Kobe, Hyogo, Japan 14 4Department of Cellular and Molecular Function Analysis, Kanazawa University Graduate 15 School of Medical Sciences, Kanazawa, Ishikawa, Japan 16 5Department of Dermatology, Kanazawa University Graduate School of Medical Sciences, 17 Kanazawa, Ishikawa, Japan 18 6Department of Vascular Physiology, Kanazawa University Graduate School of Medical 19 Sciences, Japan 20 7WPI Nano Life Science Institute, Kanazawa University, Kanazawa, Ishikawa, Japan 21 22 Running head: Neutrophil and CSF1 in progressive pulmonary fibrosis 23 24 Corresponding Author: 25 Satoshi Watanabe, MD, PhD 26 Department of Respiratory Medicine, 27 Kanazawa University Graduate School of Medical Sciences 28 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8641, Japan 29 Tel: +81-076-265-2146 30 Fax: +81-076-234-4215 31 E-mail: swatanabe@staff.kanazawa-u.ac.jp 32 33



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    Disruption of the NEDD4-AQP5 axis impairs barrier function and promotes cytokine release. (A) MUC5AC amounts (pg/mL) in cellular culture supernatants were assayed with <t>ELISA</t> across various treatment groups. (B) TER (Ω·cm²) of HNEpC monolayers was measured utilizing a Millicell ERS-2 system across the specified groups. (C, D) The amounts of IL-6 (pg/mL) <t>and</t> <t>IL-8</t> (pg/mL) in cellular culture supernatants were determined via ELISA. Data are shown as mean ± SD from three independent experiments, with each sample measured in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Ctrl group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the HDM group. Ctrl, untreated control; HDM, house dust mite-sensitized HNEpCs; HDM + siNEDD4, HDM-sensitized HNEpCs transfected with siNEDD4; HDM + ovAQP5, HDM-sensitized HNEpCs transfected with AQP5 overexpression plasmid.
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    T-cell migration and activity. A T-cell migration towards a FCS gradient or conditioned medium from B16BL6 melanoma cells. Pan T-cells were isolated from the spleens of wildtype (WT) and IKKε KO mice and plated into cell culture insert filled with T-cell medium containing 0.1% FCS. The bottom chamber was filled either with medium containing 0.1% FCS (neg ctrl), 10% FCS (pos ctrl) or conditioned medium from B16BL6 cells without FCS (B16). Cell migration of different T-cell subtypes to the lower channel was determined by FACS analysis. Cell numbers were normalized to counting beads and negative controls. n = 3–6. One-way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, ** P < 0.01. B — D Effects of co-culture of B16BL6 melanoma cells with CD8⁺ and γδ-T-cells, respectively, derived from the spleens of wildtype (WT) and IKKε KO mice. B Cell proliferation determined by WST assay, B16BL6 monocultures were set as 1 for better comparison. One-way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, **P < 0.01 significant difference in comparison to B16BL6 monoculture ( C ) IFN-γ levels analyzed by cytokine bead array (CBA) and ( D ) granzyme B release determined by ELISA. n = 4–10/group. C and D The T-cell monocultures of wildtype and IKKε knock-out mice were set as 1 for better comparison. Student´s t-test, * P < 0.05, significant difference in comparison to wildtype, # P < 0.05, ## P < 0.01, ### P < 0.001 significant difference in comparison to T-cell monocultures

    Journal: Molecular Medicine

    Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity

    doi: 10.1186/s10020-026-01651-w

    Figure Lengend Snippet: T-cell migration and activity. A T-cell migration towards a FCS gradient or conditioned medium from B16BL6 melanoma cells. Pan T-cells were isolated from the spleens of wildtype (WT) and IKKε KO mice and plated into cell culture insert filled with T-cell medium containing 0.1% FCS. The bottom chamber was filled either with medium containing 0.1% FCS (neg ctrl), 10% FCS (pos ctrl) or conditioned medium from B16BL6 cells without FCS (B16). Cell migration of different T-cell subtypes to the lower channel was determined by FACS analysis. Cell numbers were normalized to counting beads and negative controls. n = 3–6. One-way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, ** P < 0.01. B — D Effects of co-culture of B16BL6 melanoma cells with CD8⁺ and γδ-T-cells, respectively, derived from the spleens of wildtype (WT) and IKKε KO mice. B Cell proliferation determined by WST assay, B16BL6 monocultures were set as 1 for better comparison. One-way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, **P < 0.01 significant difference in comparison to B16BL6 monoculture ( C ) IFN-γ levels analyzed by cytokine bead array (CBA) and ( D ) granzyme B release determined by ELISA. n = 4–10/group. C and D The T-cell monocultures of wildtype and IKKε knock-out mice were set as 1 for better comparison. Student´s t-test, * P < 0.05, significant difference in comparison to wildtype, # P < 0.05, ## P < 0.01, ### P < 0.001 significant difference in comparison to T-cell monocultures

    Article Snippet: Granzyme B, Cxcl1, Cxcl2 and VEGF protein levels were determined from cell and tumor protein lysates using Mouse Granzyme B ELISA Kit (Invitrogen/Thermo Fisher Scientific, Dreieich, Germany #BMS6079), Mouse CXCL1/KC Quantikine ELISA Kit (#MKC00B), Mouse CXCL2/MIP-2 DuoSet ELISA (#DY452) and Mouse VEGF Quantikine ELISA Kit (#MMVOO-1) (all R&D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions.

    Techniques: Migration, Activity Assay, Isolation, Cell Culture, Analysis, Counting, Co-culture Assay, Derivative Assay, WST Assay, Comparison, Enzyme-linked Immunosorbent Assay, Knock-Out

    Regulation of cytokines and angiogenesis markers in the tumor. A OLINK proteomic analysis of inflammatory cytokines and chemokines in the tumors of wildtype and IKKε KO mice at the end of the experiment. Heat map and Volcano Plot of significantly different regulations. n = 4/group ( B ) Cxcl1 and Cxcl2 ELISA. Cxcl1: WT n = 8, IKKε-KO n = 6; Cxcl2: WT n = 6, IKKε-KO n = 5. C Determination of CD31-positive cells as vessel markers in the tumors. Left panel: FACS analysis, n = 6 per group. Right panel: immunofluorescence images and quantitative analysis, n = 4–5 tumors per group; 3–5 areas per tumor were evaluated. CD31-positive areas were normalized to wildtype controls to facilitate comparison. D MMP-9 expression as assessed by Western blot and zymography, n = 5–6 per group. E HIF-1α expression as determined by immunofluorescence, the pictures show on representative stain from 6 independent analyses, the diagram shows the densitometric analysis of all experiments, scale bar represents 100 µm. F VEGF expression analyzed by ELISA and Western Blot, n = 3/group. Student´s t-test, * P < 0.05, ** P < 0.01

    Journal: Molecular Medicine

    Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity

    doi: 10.1186/s10020-026-01651-w

    Figure Lengend Snippet: Regulation of cytokines and angiogenesis markers in the tumor. A OLINK proteomic analysis of inflammatory cytokines and chemokines in the tumors of wildtype and IKKε KO mice at the end of the experiment. Heat map and Volcano Plot of significantly different regulations. n = 4/group ( B ) Cxcl1 and Cxcl2 ELISA. Cxcl1: WT n = 8, IKKε-KO n = 6; Cxcl2: WT n = 6, IKKε-KO n = 5. C Determination of CD31-positive cells as vessel markers in the tumors. Left panel: FACS analysis, n = 6 per group. Right panel: immunofluorescence images and quantitative analysis, n = 4–5 tumors per group; 3–5 areas per tumor were evaluated. CD31-positive areas were normalized to wildtype controls to facilitate comparison. D MMP-9 expression as assessed by Western blot and zymography, n = 5–6 per group. E HIF-1α expression as determined by immunofluorescence, the pictures show on representative stain from 6 independent analyses, the diagram shows the densitometric analysis of all experiments, scale bar represents 100 µm. F VEGF expression analyzed by ELISA and Western Blot, n = 3/group. Student´s t-test, * P < 0.05, ** P < 0.01

    Article Snippet: Granzyme B, Cxcl1, Cxcl2 and VEGF protein levels were determined from cell and tumor protein lysates using Mouse Granzyme B ELISA Kit (Invitrogen/Thermo Fisher Scientific, Dreieich, Germany #BMS6079), Mouse CXCL1/KC Quantikine ELISA Kit (#MKC00B), Mouse CXCL2/MIP-2 DuoSet ELISA (#DY452) and Mouse VEGF Quantikine ELISA Kit (#MMVOO-1) (all R&D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions.

    Techniques: Analysis, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Comparison, Expressing, Western Blot, Zymography, Staining

    Early regulation of cytokines and angiogenesis markers in the tumor. A Cxcl1 ELISA, ( B ) MMP-9 expression (Western Blot and zymography), ( C ) HIF-1α expression as determined by immunofluorescence. The pictures show a representative stain from 4–6 independent analyses, the diagram shows the densitometric analysis of all experiments, scale bar represents 100 µm. D FACS analysis of CD31. + -cells as vessel markers in the tumors, ( n = 6/group). E VEGF expression analyzed by ELISA and Western Blot, ( F ) p65 expression and activity in nuclear extracts as determined by Western Blot and transcription factor ELISA. n = 3–5/group. Student´s t-test, * P < 0.05, ** P < 0.01

    Journal: Molecular Medicine

    Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity

    doi: 10.1186/s10020-026-01651-w

    Figure Lengend Snippet: Early regulation of cytokines and angiogenesis markers in the tumor. A Cxcl1 ELISA, ( B ) MMP-9 expression (Western Blot and zymography), ( C ) HIF-1α expression as determined by immunofluorescence. The pictures show a representative stain from 4–6 independent analyses, the diagram shows the densitometric analysis of all experiments, scale bar represents 100 µm. D FACS analysis of CD31. + -cells as vessel markers in the tumors, ( n = 6/group). E VEGF expression analyzed by ELISA and Western Blot, ( F ) p65 expression and activity in nuclear extracts as determined by Western Blot and transcription factor ELISA. n = 3–5/group. Student´s t-test, * P < 0.05, ** P < 0.01

    Article Snippet: Granzyme B, Cxcl1, Cxcl2 and VEGF protein levels were determined from cell and tumor protein lysates using Mouse Granzyme B ELISA Kit (Invitrogen/Thermo Fisher Scientific, Dreieich, Germany #BMS6079), Mouse CXCL1/KC Quantikine ELISA Kit (#MKC00B), Mouse CXCL2/MIP-2 DuoSet ELISA (#DY452) and Mouse VEGF Quantikine ELISA Kit (#MMVOO-1) (all R&D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Zymography, Immunofluorescence, Staining, Analysis, Activity Assay

    Angiogenesis assay in HUVEC with and without depletion of IKKε by siRNA. A IKKε mRNA and protein expression in HUVEC cells after treatment with IKKε siRNA or a scrambled negative control as determined by PCR and Western Blot analysis, PCR: n = 3–4/group, Western Blot ( n = 2/group) ( B ) Tube formation assay, left side: microscopic pictures, right side: analysis of the total mesh area of naïve HUVEC, or HUVEC treated with NC and IKKε siRNA. n = 6–7/group. The scale bars represent 100 µm. C Spheroid sprout assay, left side: microscopic pictures, right side: analysis of cumulative sprout length (CSL) in spheroids of naïve HUVEC ( n = 15 spheroids), HUVEC treated with VEGF ( n = 38 spheroids) HUVEC-NC ( n = 25 spheroids) and HUVEC-IKKε siRNA ( n = 38 spheroids). Data were generated in 5 independent biological experiments. The scale bars represent 100 µm. One Way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Journal: Molecular Medicine

    Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity

    doi: 10.1186/s10020-026-01651-w

    Figure Lengend Snippet: Angiogenesis assay in HUVEC with and without depletion of IKKε by siRNA. A IKKε mRNA and protein expression in HUVEC cells after treatment with IKKε siRNA or a scrambled negative control as determined by PCR and Western Blot analysis, PCR: n = 3–4/group, Western Blot ( n = 2/group) ( B ) Tube formation assay, left side: microscopic pictures, right side: analysis of the total mesh area of naïve HUVEC, or HUVEC treated with NC and IKKε siRNA. n = 6–7/group. The scale bars represent 100 µm. C Spheroid sprout assay, left side: microscopic pictures, right side: analysis of cumulative sprout length (CSL) in spheroids of naïve HUVEC ( n = 15 spheroids), HUVEC treated with VEGF ( n = 38 spheroids) HUVEC-NC ( n = 25 spheroids) and HUVEC-IKKε siRNA ( n = 38 spheroids). Data were generated in 5 independent biological experiments. The scale bars represent 100 µm. One Way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Article Snippet: Granzyme B, Cxcl1, Cxcl2 and VEGF protein levels were determined from cell and tumor protein lysates using Mouse Granzyme B ELISA Kit (Invitrogen/Thermo Fisher Scientific, Dreieich, Germany #BMS6079), Mouse CXCL1/KC Quantikine ELISA Kit (#MKC00B), Mouse CXCL2/MIP-2 DuoSet ELISA (#DY452) and Mouse VEGF Quantikine ELISA Kit (#MMVOO-1) (all R&D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions.

    Techniques: Angiogenesis Assay, Expressing, Negative Control, Western Blot, Analysis, Tube Formation Assay, Spheroids, Generated

    Disruption of the NEDD4-AQP5 axis impairs barrier function and promotes cytokine release. (A) MUC5AC amounts (pg/mL) in cellular culture supernatants were assayed with ELISA across various treatment groups. (B) TER (Ω·cm²) of HNEpC monolayers was measured utilizing a Millicell ERS-2 system across the specified groups. (C, D) The amounts of IL-6 (pg/mL) and IL-8 (pg/mL) in cellular culture supernatants were determined via ELISA. Data are shown as mean ± SD from three independent experiments, with each sample measured in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Ctrl group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the HDM group. Ctrl, untreated control; HDM, house dust mite-sensitized HNEpCs; HDM + siNEDD4, HDM-sensitized HNEpCs transfected with siNEDD4; HDM + ovAQP5, HDM-sensitized HNEpCs transfected with AQP5 overexpression plasmid.

    Journal: Frontiers in Immunology

    Article Title: The role of NEDD4-mediated ubiquitination of AQP5 in allergic rhinitis: implications for nasal mucosal barrier function

    doi: 10.3389/fimmu.2026.1908261

    Figure Lengend Snippet: Disruption of the NEDD4-AQP5 axis impairs barrier function and promotes cytokine release. (A) MUC5AC amounts (pg/mL) in cellular culture supernatants were assayed with ELISA across various treatment groups. (B) TER (Ω·cm²) of HNEpC monolayers was measured utilizing a Millicell ERS-2 system across the specified groups. (C, D) The amounts of IL-6 (pg/mL) and IL-8 (pg/mL) in cellular culture supernatants were determined via ELISA. Data are shown as mean ± SD from three independent experiments, with each sample measured in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Ctrl group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the HDM group. Ctrl, untreated control; HDM, house dust mite-sensitized HNEpCs; HDM + siNEDD4, HDM-sensitized HNEpCs transfected with siNEDD4; HDM + ovAQP5, HDM-sensitized HNEpCs transfected with AQP5 overexpression plasmid.

    Article Snippet: Cell culture supernatants were used to detect IL-6, IL-8, and MUC5AC levels in the supernatant by using ELISA kits for IL-6 (R&D Systems, D6050), IL-8 (R&D Systems, D8000C), and MUC5AC (R&D Systems, DY2197).

    Techniques: Disruption, Cell Culture, Enzyme-linked Immunosorbent Assay, Control, Transfection, Over Expression, Plasmid Preparation