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qseq software  (DNASTAR)


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    Structured Review

    DNASTAR qseq software
    (A) Primary HBE cells were stimulated with media or a combination of 10/-50 ng/ml IL-22 and 50 ng/ml of IL-17A for 48 hours (n = 3 donors per condition). RNA was harvested and subjected to hybridization on Hu-133 arrays for RNA sequencing as described in the . Transcript abundance or hybridization intensity was measured on normalized data in <t>Qseq</t> <t>software</t> (Lasergene). Scatter plot analysis of normalized transcript abundance for Affymetrix (x-axis) and RNA-seq (y-axis) are shown. (B) Relative gene expression of MMPs and TIMP2 from RNA-seq data. (C) Up-regulation of Ccl2 by IL-17 and IL-22 combination treatment in primary HBE cells as measured by RNAseq. (D) CCL2, MMP3 and MMP12 gene expression were analyzed by real-time RT-PCR.
    Qseq Software, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 10381 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qseq software/product/DNASTAR
    Average 99 stars, based on 10381 article reviews
    qseq software - by Bioz Stars, 2026-05
    99/100 stars

    Images

    1) Product Images from "IL-17RA Is Required for CCL2 Expression, Macrophage Recruitment, and Emphysema in Response to Cigarette Smoke"

    Article Title: IL-17RA Is Required for CCL2 Expression, Macrophage Recruitment, and Emphysema in Response to Cigarette Smoke

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0020333

    (A) Primary HBE cells were stimulated with media or a combination of 10/-50 ng/ml IL-22 and 50 ng/ml of IL-17A for 48 hours (n = 3 donors per condition). RNA was harvested and subjected to hybridization on Hu-133 arrays for RNA sequencing as described in the . Transcript abundance or hybridization intensity was measured on normalized data in Qseq software (Lasergene). Scatter plot analysis of normalized transcript abundance for Affymetrix (x-axis) and RNA-seq (y-axis) are shown. (B) Relative gene expression of MMPs and TIMP2 from RNA-seq data. (C) Up-regulation of Ccl2 by IL-17 and IL-22 combination treatment in primary HBE cells as measured by RNAseq. (D) CCL2, MMP3 and MMP12 gene expression were analyzed by real-time RT-PCR.
    Figure Legend Snippet: (A) Primary HBE cells were stimulated with media or a combination of 10/-50 ng/ml IL-22 and 50 ng/ml of IL-17A for 48 hours (n = 3 donors per condition). RNA was harvested and subjected to hybridization on Hu-133 arrays for RNA sequencing as described in the . Transcript abundance or hybridization intensity was measured on normalized data in Qseq software (Lasergene). Scatter plot analysis of normalized transcript abundance for Affymetrix (x-axis) and RNA-seq (y-axis) are shown. (B) Relative gene expression of MMPs and TIMP2 from RNA-seq data. (C) Up-regulation of Ccl2 by IL-17 and IL-22 combination treatment in primary HBE cells as measured by RNAseq. (D) CCL2, MMP3 and MMP12 gene expression were analyzed by real-time RT-PCR.

    Techniques Used: Hybridization, RNA Sequencing Assay, Software, Expressing, Quantitative RT-PCR



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    (A) Primary HBE cells were stimulated with media or a combination of 10/-50 ng/ml IL-22 and 50 ng/ml of IL-17A for 48 hours (n = 3 donors per condition). RNA was harvested and subjected to hybridization on Hu-133 arrays for RNA sequencing as described in the . Transcript abundance or hybridization intensity was measured on normalized data in <t>Qseq</t> <t>software</t> (Lasergene). Scatter plot analysis of normalized transcript abundance for Affymetrix (x-axis) and RNA-seq (y-axis) are shown. (B) Relative gene expression of MMPs and TIMP2 from RNA-seq data. (C) Up-regulation of Ccl2 by IL-17 and IL-22 combination treatment in primary HBE cells as measured by RNAseq. (D) CCL2, MMP3 and MMP12 gene expression were analyzed by real-time RT-PCR.
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    (A) Primary HBE cells were stimulated with media or a combination of 10/-50 ng/ml IL-22 and 50 ng/ml of IL-17A for 48 hours (n = 3 donors per condition). RNA was harvested and subjected to hybridization on Hu-133 arrays for RNA sequencing as described in the . Transcript abundance or hybridization intensity was measured on normalized data in <t>Qseq</t> <t>software</t> (Lasergene). Scatter plot analysis of normalized transcript abundance for Affymetrix (x-axis) and RNA-seq (y-axis) are shown. (B) Relative gene expression of MMPs and TIMP2 from RNA-seq data. (C) Up-regulation of Ccl2 by IL-17 and IL-22 combination treatment in primary HBE cells as measured by RNAseq. (D) CCL2, MMP3 and MMP12 gene expression were analyzed by real-time RT-PCR.
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    qseq software version 12  (DNASTAR)
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    (A) Primary HBE cells were stimulated with media or a combination of 10/-50 ng/ml IL-22 and 50 ng/ml of IL-17A for 48 hours (n = 3 donors per condition). RNA was harvested and subjected to hybridization on Hu-133 arrays for RNA sequencing as described in the . Transcript abundance or hybridization intensity was measured on normalized data in <t>Qseq</t> <t>software</t> (Lasergene). Scatter plot analysis of normalized transcript abundance for Affymetrix (x-axis) and RNA-seq (y-axis) are shown. (B) Relative gene expression of MMPs and TIMP2 from RNA-seq data. (C) Up-regulation of Ccl2 by IL-17 and IL-22 combination treatment in primary HBE cells as measured by RNAseq. (D) CCL2, MMP3 and MMP12 gene expression were analyzed by real-time RT-PCR.
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    Image Search Results


    (A) Primary HBE cells were stimulated with media or a combination of 10/-50 ng/ml IL-22 and 50 ng/ml of IL-17A for 48 hours (n = 3 donors per condition). RNA was harvested and subjected to hybridization on Hu-133 arrays for RNA sequencing as described in the . Transcript abundance or hybridization intensity was measured on normalized data in Qseq software (Lasergene). Scatter plot analysis of normalized transcript abundance for Affymetrix (x-axis) and RNA-seq (y-axis) are shown. (B) Relative gene expression of MMPs and TIMP2 from RNA-seq data. (C) Up-regulation of Ccl2 by IL-17 and IL-22 combination treatment in primary HBE cells as measured by RNAseq. (D) CCL2, MMP3 and MMP12 gene expression were analyzed by real-time RT-PCR.

    Journal: PLoS ONE

    Article Title: IL-17RA Is Required for CCL2 Expression, Macrophage Recruitment, and Emphysema in Response to Cigarette Smoke

    doi: 10.1371/journal.pone.0020333

    Figure Lengend Snippet: (A) Primary HBE cells were stimulated with media or a combination of 10/-50 ng/ml IL-22 and 50 ng/ml of IL-17A for 48 hours (n = 3 donors per condition). RNA was harvested and subjected to hybridization on Hu-133 arrays for RNA sequencing as described in the . Transcript abundance or hybridization intensity was measured on normalized data in Qseq software (Lasergene). Scatter plot analysis of normalized transcript abundance for Affymetrix (x-axis) and RNA-seq (y-axis) are shown. (B) Relative gene expression of MMPs and TIMP2 from RNA-seq data. (C) Up-regulation of Ccl2 by IL-17 and IL-22 combination treatment in primary HBE cells as measured by RNAseq. (D) CCL2, MMP3 and MMP12 gene expression were analyzed by real-time RT-PCR.

    Article Snippet: Qseq software (Lasergene) was used to determine the relative transcript abundance.

    Techniques: Hybridization, RNA Sequencing Assay, Software, Expressing, Quantitative RT-PCR