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Image <t>analysis</t> of Masson's trichrome‐stained lung sections for the <t>quantification</t> of airway smooth muscle (ASM; C) and collagen content (D) <t>relative</t> to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).
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Image <t>analysis</t> of Masson's trichrome‐stained lung sections for the <t>quantification</t> of airway smooth muscle (ASM; C) and collagen content (D) <t>relative</t> to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).
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qbase relative quantification software  (Biogazelle)
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Biogazelle qbase relative quantification software
Image <t>analysis</t> of Masson's trichrome‐stained lung sections for the <t>quantification</t> of airway smooth muscle (ASM; C) and collagen content (D) <t>relative</t> to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).
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https://www.bioz.com/result/qbase relative quantification software/product/Biogazelle
Average 90 stars, based on 1 article reviews
qbase relative quantification software - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

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Image analysis of Masson's trichrome‐stained lung sections for the quantification of airway smooth muscle (ASM; C) and collagen content (D) relative to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).

Journal: The Journal of Physiology

Article Title: Development of an experimental model of maternal allergic asthma during pregnancy

doi: 10.1113/JP270752

Figure Lengend Snippet: Image analysis of Masson's trichrome‐stained lung sections for the quantification of airway smooth muscle (ASM; C) and collagen content (D) relative to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).

Article Snippet: The reference genes (β actin, hypoxanthine phosphoribosyltransferase 1 and tyrosine 3‐monooxygenase (YWAHZ)) were chosen (Duffield et al . 2009 ; Passmore et al . 2009 ; Wang et al . 2013 ; Hellemans & Vandesompele, 2014 ) based on expression analysis using the geNorm component of the qBase (Biogazelle, Zwinjnaarde, Belgium) relative quantification analysis software (Hellemans et al . 2007 ) because their expression was stable across samples (maximum value, factor = 0.3–0.4; Vandesompele et al . 2002 ; Soo et al . 2012 ; Hellemans & Vandesompele, 2014 ).

Techniques: Staining, Membrane, Control