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Pyrosequencing Inc pyrosequencing based analysis
Pyrosequencing Based Analysis, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pyrosequencing-based+assays/16s+pyrosequencing+rrna/pm42128334-658-12-12
Average 86 stars, based on 1 article reviews
pyrosequencing based analysis - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Diagnostic Assay:

Article Title: Non-canonical IDH1 and IDH2 mutations: a clonal and relevant event in an Italian cohort of gliomas classified according to the 2016 World Health Organization (WHO) criteria.
Article Snippet: 8/31, 25.8%; oligodendrogliomas: 5/21, 23.8%) and in 5/40 (12.5%) grade III gliomas (astrocytomas: 3/25, 12.0%; oligodendrogliomas: 2/15, 13.3%).. They were not identified in 196 grade IV gliomas (192 glioblastomas, 4 gliosarcomas).. In the large majority (>80%) of tumors IDH mutations, both IDH1-R132H and the non-canonical ones, were present in the large majority (>80%) of neoplastic cells.

Article Title: Association between blood pressure and DNA methylation of retrotransposons and pro-inflammatory genes
Article Snippet: Pyrosequencing-based assays produce individual measures of methylation at more than one CpG dinucleotide.

Article Title: Update on Influenza Diagnostics: Lessons from the Novel H1N1 Influenza A Pandemic
Article Snippet: Pyrosequencing-based assays have some limitations as well. (i) While markers of adamantane resistance in influenza virus are well established (mutations at residues 26, 27, 30, 31, and 34 in the M2 protein), molecular determinants of NAI resistance are not as well characterized.

Article Title: A pyrosequencing assay for the rapid discrimination of mitochondrial lineages in the Salmo trutta species complex.
Article Snippet: The European trout (Salmo trutta species complex) is genetically very diverse consisting of five distinct mitochondrial lineages that probably originated in the Pleistocene.. Here, we describe a novel pyrosequencing protocol to generate two short sequence reads from the mitochondrial control region, which allow the unambiguous identification of all five lineages.. The approach was found to be easily transferable between laboratories and should be a valuable tool for the assessment of genetic diversity in trout.

Article Title: Promoter Methylation of Selected Genes in Non-Small-Cell Lung Cancer Patients and Cell Lines
Article Snippet: Pyrosequencing-based assays were developed and validated for clinical use to detect the DNA promoter methylation of five genes.

Article Title: Satisfying the need for rapid diagnosis of new variant influenza A H1N1.
Article Snippet: Pyrosequencing-based assays are, however, highly contamination sensitive, and require instrumentation not present at many diagnostic laboratories.

Article Title: Molecular heterogeneity characterizes glioblastoma with lipoblast/adipocyte-like cytology.
Article Snippet: Glioblastoma, the most common primary brain tumor in adults, may rarely show among unusual histological patterns lipoblast/adipocyte-like features.. The genetic features of such cases are not yet well characterized, and molecular data are available for only few cases.. In order to further expand our knowledge about their molecular profile, we analyzed four cases of glioblastoma with adipocyte-like features.

Article Title: Tissue-specific DNA methylation profiles regulate liver-specific expression of the APOA1/C3/A4/A5 cluster and can be manipulated with demethylating agents on intestinal cells.
Article Snippet: Objective: The tissue-specific expression profiles of genes within the APOA1/C3/A4/A5 cluster play an important role in lipid metabolism regulation.. We hypothesize that the tissue-specific expression of the APOA1/C3/A4/A5 gene cluster will show an inverse pattern with DNA methylation, and that repression in nonor low-expressing tissue, such as the intestine, can be reversed using epigenetic drugs.. Methods and results: We analyzed DNA samples from different human adult tissues (liver, intestine, leukocytes, brain, kidney, pancreas, muscle and sperm) using the Infinium HumanMethyation450 BeadChip array.

Mutagenesis:

Article Title: Non-canonical IDH1 and IDH2 mutations: a clonal and relevant event in an Italian cohort of gliomas classified according to the 2016 World Health Organization (WHO) criteria.
Article Snippet: 8/31, 25.8%; oligodendrogliomas: 5/21, 23.8%) and in 5/40 (12.5%) grade III gliomas (astrocytomas: 3/25, 12.0%; oligodendrogliomas: 2/15, 13.3%).. They were not identified in 196 grade IV gliomas (192 glioblastomas, 4 gliosarcomas).. In the large majority (>80%) of tumors IDH mutations, both IDH1-R132H and the non-canonical ones, were present in the large majority (>80%) of neoplastic cells.

Article Title: Association between blood pressure and DNA methylation of retrotransposons and pro-inflammatory genes
Article Snippet: Pyrosequencing-based assays produce individual measures of methylation at more than one CpG dinucleotide.

Article Title: Update on Influenza Diagnostics: Lessons from the Novel H1N1 Influenza A Pandemic
Article Snippet: Pyrosequencing-based assays have some limitations as well. (i) While markers of adamantane resistance in influenza virus are well established (mutations at residues 26, 27, 30, 31, and 34 in the M2 protein), molecular determinants of NAI resistance are not as well characterized.

Article Title: A pyrosequencing assay for the rapid discrimination of mitochondrial lineages in the Salmo trutta species complex.
Article Snippet: The European trout (Salmo trutta species complex) is genetically very diverse consisting of five distinct mitochondrial lineages that probably originated in the Pleistocene.. Here, we describe a novel pyrosequencing protocol to generate two short sequence reads from the mitochondrial control region, which allow the unambiguous identification of all five lineages.. The approach was found to be easily transferable between laboratories and should be a valuable tool for the assessment of genetic diversity in trout.

Article Title: Promoter Methylation of Selected Genes in Non-Small-Cell Lung Cancer Patients and Cell Lines
Article Snippet: Pyrosequencing-based assays were developed and validated for clinical use to detect the DNA promoter methylation of five genes.

Article Title: Satisfying the need for rapid diagnosis of new variant influenza A H1N1.
Article Snippet: Pyrosequencing-based assays are, however, highly contamination sensitive, and require instrumentation not present at many diagnostic laboratories.

Article Title: Molecular heterogeneity characterizes glioblastoma with lipoblast/adipocyte-like cytology.
Article Snippet: Glioblastoma, the most common primary brain tumor in adults, may rarely show among unusual histological patterns lipoblast/adipocyte-like features.. The genetic features of such cases are not yet well characterized, and molecular data are available for only few cases.. In order to further expand our knowledge about their molecular profile, we analyzed four cases of glioblastoma with adipocyte-like features.

Article Title: Tissue-specific DNA methylation profiles regulate liver-specific expression of the APOA1/C3/A4/A5 cluster and can be manipulated with demethylating agents on intestinal cells.
Article Snippet: Objective: The tissue-specific expression profiles of genes within the APOA1/C3/A4/A5 cluster play an important role in lipid metabolism regulation.. We hypothesize that the tissue-specific expression of the APOA1/C3/A4/A5 gene cluster will show an inverse pattern with DNA methylation, and that repression in nonor low-expressing tissue, such as the intestine, can be reversed using epigenetic drugs.. Methods and results: We analyzed DNA samples from different human adult tissues (liver, intestine, leukocytes, brain, kidney, pancreas, muscle and sperm) using the Infinium HumanMethyation450 BeadChip array.

Polymerase Chain Reaction:

Article Title: Non-canonical IDH1 and IDH2 mutations: a clonal and relevant event in an Italian cohort of gliomas classified according to the 2016 World Health Organization (WHO) criteria.
Article Snippet: 8/31, 25.8%; oligodendrogliomas: 5/21, 23.8%) and in 5/40 (12.5%) grade III gliomas (astrocytomas: 3/25, 12.0%; oligodendrogliomas: 2/15, 13.3%).. They were not identified in 196 grade IV gliomas (192 glioblastomas, 4 gliosarcomas).. In the large majority (>80%) of tumors IDH mutations, both IDH1-R132H and the non-canonical ones, were present in the large majority (>80%) of neoplastic cells.

Article Title: Association between blood pressure and DNA methylation of retrotransposons and pro-inflammatory genes
Article Snippet: Pyrosequencing-based assays produce individual measures of methylation at more than one CpG dinucleotide.

Article Title: Update on Influenza Diagnostics: Lessons from the Novel H1N1 Influenza A Pandemic
Article Snippet: Pyrosequencing-based assays have some limitations as well. (i) While markers of adamantane resistance in influenza virus are well established (mutations at residues 26, 27, 30, 31, and 34 in the M2 protein), molecular determinants of NAI resistance are not as well characterized.

Article Title: A pyrosequencing assay for the rapid discrimination of mitochondrial lineages in the Salmo trutta species complex.
Article Snippet: The European trout (Salmo trutta species complex) is genetically very diverse consisting of five distinct mitochondrial lineages that probably originated in the Pleistocene.. Here, we describe a novel pyrosequencing protocol to generate two short sequence reads from the mitochondrial control region, which allow the unambiguous identification of all five lineages.. The approach was found to be easily transferable between laboratories and should be a valuable tool for the assessment of genetic diversity in trout.

Article Title: Promoter Methylation of Selected Genes in Non-Small-Cell Lung Cancer Patients and Cell Lines
Article Snippet: Pyrosequencing-based assays were developed and validated for clinical use to detect the DNA promoter methylation of five genes.

Article Title: Satisfying the need for rapid diagnosis of new variant influenza A H1N1.
Article Snippet: Pyrosequencing-based assays are, however, highly contamination sensitive, and require instrumentation not present at many diagnostic laboratories.

Article Title: Molecular heterogeneity characterizes glioblastoma with lipoblast/adipocyte-like cytology.
Article Snippet: Glioblastoma, the most common primary brain tumor in adults, may rarely show among unusual histological patterns lipoblast/adipocyte-like features.. The genetic features of such cases are not yet well characterized, and molecular data are available for only few cases.. In order to further expand our knowledge about their molecular profile, we analyzed four cases of glioblastoma with adipocyte-like features.

Article Title: Tissue-specific DNA methylation profiles regulate liver-specific expression of the APOA1/C3/A4/A5 cluster and can be manipulated with demethylating agents on intestinal cells.
Article Snippet: Objective: The tissue-specific expression profiles of genes within the APOA1/C3/A4/A5 cluster play an important role in lipid metabolism regulation.. We hypothesize that the tissue-specific expression of the APOA1/C3/A4/A5 gene cluster will show an inverse pattern with DNA methylation, and that repression in nonor low-expressing tissue, such as the intestine, can be reversed using epigenetic drugs.. Methods and results: We analyzed DNA samples from different human adult tissues (liver, intestine, leukocytes, brain, kidney, pancreas, muscle and sperm) using the Infinium HumanMethyation450 BeadChip array.

Genome Wide:

Article Title: Non-canonical IDH1 and IDH2 mutations: a clonal and relevant event in an Italian cohort of gliomas classified according to the 2016 World Health Organization (WHO) criteria.
Article Snippet: 8/31, 25.8%; oligodendrogliomas: 5/21, 23.8%) and in 5/40 (12.5%) grade III gliomas (astrocytomas: 3/25, 12.0%; oligodendrogliomas: 2/15, 13.3%).. They were not identified in 196 grade IV gliomas (192 glioblastomas, 4 gliosarcomas).. In the large majority (>80%) of tumors IDH mutations, both IDH1-R132H and the non-canonical ones, were present in the large majority (>80%) of neoplastic cells.

Article Title: Association between blood pressure and DNA methylation of retrotransposons and pro-inflammatory genes
Article Snippet: Pyrosequencing-based assays produce individual measures of methylation at more than one CpG dinucleotide.

Article Title: Update on Influenza Diagnostics: Lessons from the Novel H1N1 Influenza A Pandemic
Article Snippet: Pyrosequencing-based assays have some limitations as well. (i) While markers of adamantane resistance in influenza virus are well established (mutations at residues 26, 27, 30, 31, and 34 in the M2 protein), molecular determinants of NAI resistance are not as well characterized.

Article Title: A pyrosequencing assay for the rapid discrimination of mitochondrial lineages in the Salmo trutta species complex.
Article Snippet: The European trout (Salmo trutta species complex) is genetically very diverse consisting of five distinct mitochondrial lineages that probably originated in the Pleistocene.. Here, we describe a novel pyrosequencing protocol to generate two short sequence reads from the mitochondrial control region, which allow the unambiguous identification of all five lineages.. The approach was found to be easily transferable between laboratories and should be a valuable tool for the assessment of genetic diversity in trout.

Article Title: Promoter Methylation of Selected Genes in Non-Small-Cell Lung Cancer Patients and Cell Lines
Article Snippet: Pyrosequencing-based assays were developed and validated for clinical use to detect the DNA promoter methylation of five genes.

Article Title: Satisfying the need for rapid diagnosis of new variant influenza A H1N1.
Article Snippet: Pyrosequencing-based assays are, however, highly contamination sensitive, and require instrumentation not present at many diagnostic laboratories.

Article Title: Molecular heterogeneity characterizes glioblastoma with lipoblast/adipocyte-like cytology.
Article Snippet: Glioblastoma, the most common primary brain tumor in adults, may rarely show among unusual histological patterns lipoblast/adipocyte-like features.. The genetic features of such cases are not yet well characterized, and molecular data are available for only few cases.. In order to further expand our knowledge about their molecular profile, we analyzed four cases of glioblastoma with adipocyte-like features.

Article Title: Tissue-specific DNA methylation profiles regulate liver-specific expression of the APOA1/C3/A4/A5 cluster and can be manipulated with demethylating agents on intestinal cells.
Article Snippet: Objective: The tissue-specific expression profiles of genes within the APOA1/C3/A4/A5 cluster play an important role in lipid metabolism regulation.. We hypothesize that the tissue-specific expression of the APOA1/C3/A4/A5 gene cluster will show an inverse pattern with DNA methylation, and that repression in nonor low-expressing tissue, such as the intestine, can be reversed using epigenetic drugs.. Methods and results: We analyzed DNA samples from different human adult tissues (liver, intestine, leukocytes, brain, kidney, pancreas, muscle and sperm) using the Infinium HumanMethyation450 BeadChip array.

Methylation:

Article Title: Non-canonical IDH1 and IDH2 mutations: a clonal and relevant event in an Italian cohort of gliomas classified according to the 2016 World Health Organization (WHO) criteria.
Article Snippet: 8/31, 25.8%; oligodendrogliomas: 5/21, 23.8%) and in 5/40 (12.5%) grade III gliomas (astrocytomas: 3/25, 12.0%; oligodendrogliomas: 2/15, 13.3%).. They were not identified in 196 grade IV gliomas (192 glioblastomas, 4 gliosarcomas).. In the large majority (>80%) of tumors IDH mutations, both IDH1-R132H and the non-canonical ones, were present in the large majority (>80%) of neoplastic cells.

Article Title: Association between blood pressure and DNA methylation of retrotransposons and pro-inflammatory genes
Article Snippet: Pyrosequencing-based assays produce individual measures of methylation at more than one CpG dinucleotide.

Article Title: Update on Influenza Diagnostics: Lessons from the Novel H1N1 Influenza A Pandemic
Article Snippet: Pyrosequencing-based assays have some limitations as well. (i) While markers of adamantane resistance in influenza virus are well established (mutations at residues 26, 27, 30, 31, and 34 in the M2 protein), molecular determinants of NAI resistance are not as well characterized.

Article Title: A pyrosequencing assay for the rapid discrimination of mitochondrial lineages in the Salmo trutta species complex.
Article Snippet: The European trout (Salmo trutta species complex) is genetically very diverse consisting of five distinct mitochondrial lineages that probably originated in the Pleistocene.. Here, we describe a novel pyrosequencing protocol to generate two short sequence reads from the mitochondrial control region, which allow the unambiguous identification of all five lineages.. The approach was found to be easily transferable between laboratories and should be a valuable tool for the assessment of genetic diversity in trout.

Article Title: Promoter Methylation of Selected Genes in Non-Small-Cell Lung Cancer Patients and Cell Lines
Article Snippet: Pyrosequencing-based assays were developed and validated for clinical use to detect the DNA promoter methylation of five genes.

Article Title: Satisfying the need for rapid diagnosis of new variant influenza A H1N1.
Article Snippet: Pyrosequencing-based assays are, however, highly contamination sensitive, and require instrumentation not present at many diagnostic laboratories.

Article Title: Molecular heterogeneity characterizes glioblastoma with lipoblast/adipocyte-like cytology.
Article Snippet: Glioblastoma, the most common primary brain tumor in adults, may rarely show among unusual histological patterns lipoblast/adipocyte-like features.. The genetic features of such cases are not yet well characterized, and molecular data are available for only few cases.. In order to further expand our knowledge about their molecular profile, we analyzed four cases of glioblastoma with adipocyte-like features.

Article Title: Tissue-specific DNA methylation profiles regulate liver-specific expression of the APOA1/C3/A4/A5 cluster and can be manipulated with demethylating agents on intestinal cells.
Article Snippet: Objective: The tissue-specific expression profiles of genes within the APOA1/C3/A4/A5 cluster play an important role in lipid metabolism regulation.. We hypothesize that the tissue-specific expression of the APOA1/C3/A4/A5 gene cluster will show an inverse pattern with DNA methylation, and that repression in nonor low-expressing tissue, such as the intestine, can be reversed using epigenetic drugs.. Methods and results: We analyzed DNA samples from different human adult tissues (liver, intestine, leukocytes, brain, kidney, pancreas, muscle and sperm) using the Infinium HumanMethyation450 BeadChip array.



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Image Search Results


Association between progression-free survival (PFS) and the MGMTp methylation score. The association of the MGMTp methylation score, based on the MGMT-STP27 procedure, with progression-free survival (PFS; interval from diagnosis) was evaluated by the hazard ratio (confidence interval at 95%) from the cox regression model. The forest plots in A and B correspond to the PFS reported for patients treated in EORTC-22033 with temozolomide (TMZ) or radiotherapy (RT), respectively. , The MGMT-STP27 score was significant in the TMZ treated patients, but not in the patients with RT. The forest plots in C and D visualize the outcome of the Montpellier patients treated with TMZ, with time to progression defined by the next treatment-free survival (NxtTrtFS), and PFS defined by RANO, respectively. The MGMT-STP27 score was significant in the Montpellier cohort when using NxtTrtFS as outcome measure, a trend was observed when using RANO criteria for PFS. * P <.05; ** P <.01.

Journal: Neuro-Oncology Advances

Article Title: Clinical value of the MGMT promoter methylation score in IDHmt low-grade glioma for predicting benefit from temozolomide treatment

doi: 10.1093/noajnl/vdae224

Figure Lengend Snippet: Association between progression-free survival (PFS) and the MGMTp methylation score. The association of the MGMTp methylation score, based on the MGMT-STP27 procedure, with progression-free survival (PFS; interval from diagnosis) was evaluated by the hazard ratio (confidence interval at 95%) from the cox regression model. The forest plots in A and B correspond to the PFS reported for patients treated in EORTC-22033 with temozolomide (TMZ) or radiotherapy (RT), respectively. , The MGMT-STP27 score was significant in the TMZ treated patients, but not in the patients with RT. The forest plots in C and D visualize the outcome of the Montpellier patients treated with TMZ, with time to progression defined by the next treatment-free survival (NxtTrtFS), and PFS defined by RANO, respectively. The MGMT-STP27 score was significant in the Montpellier cohort when using NxtTrtFS as outcome measure, a trend was observed when using RANO criteria for PFS. * P <.05; ** P <.01.

Article Snippet: The association between the MGMT-STP27 score and NxtTrtFS was confirmed using a pyrosequencing-based MGMTp methylation score (MGMT-PYROscore) obtained for the same samples ( P = .002, ).

Techniques: Methylation, Biomarker Discovery