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rt-pcr-pyrosequencing assay  (Pyrosequencing Inc)

 
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    Pyrosequencing Inc rt-pcr-pyrosequencing assay
    Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
    Rt Pcr Pyrosequencing Assay, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pyrosequencing+rt-pcr/pcr+pyrosequencing/pmc09088475-58-9-10
    Average 90 stars, based on 1 article reviews
    rt-pcr-pyrosequencing assay - by Bioz Stars, 2026-11
    90/100 stars

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    1) Product Images from "A pyrosequencing protocol for rapid identification of SARS‐CoV‐2 variants"

    Article Title: A pyrosequencing protocol for rapid identification of SARS‐CoV‐2 variants

    Journal: Journal of Medical Virology

    doi: 10.1002/jmv.27770

    Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the S protein and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
    Figure Legend Snippet: Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the S protein and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.

    Techniques Used: Binding Assay, Sequencing

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    Article Title: Design and Validation of an H5 TaqMan Real-Time One-Step Reverse Transcription-PCR and Confirmatory Assays for Diagnosis and Verification of Influenza A Virus H5 Infections in Humans
    Article Snippet: However, amplicons from the other clade 2 H5 viruses analyzed retained the Taq I restriction site and gave restriction patterns characteristic of clade 2 viruses (Fig. 2b). (ii) Pyrosequencing RT-PCR and sequence analysis.

    Article Title: Methods for molecular surveillance of influenza
    Article Snippet: Pyrosequencing, combined with RT-PCR techniques, has provided rapid, high-throughput and cost-effective screening of NA inhibitor-resistant influenza A viruses of subtype H1N1, H3N2 and H5N1 [ 55 ].



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    Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
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    Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
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    Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
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    Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
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    Image Search Results


    Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the S protein and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.

    Journal: Journal of Medical Virology

    Article Title: A pyrosequencing protocol for rapid identification of SARS‐CoV‐2 variants

    doi: 10.1002/jmv.27770

    Figure Lengend Snippet: Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the S protein and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.

    Article Snippet: The current study describes the development of a reverse transcription (RT)‐PCR‐pyrosequencing assay targeting >65 spike protein gene ( S ) mutations of SARS‐CoV‐2, which permits differentiation of commonly reported variants currently circulating in the United States with a high degree of confidence.

    Techniques: Binding Assay, Sequencing