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tsdr pyrosequencing primer agaaatttgtggggtggg  (Pyrosequencing Inc)

 
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    Pyrosequencing Inc tsdr pyrosequencing primer agaaatttgtggggtggg
    TYK2 inhibition enhances Treg stability in Th17-polarizing conditions Tregs (CD4 + CD25 hi CD127 lo ) were isolated from peripheral blood of healthy human donors, stimulated with anti-CD3/CD28, and cultured for 7 days in a Th17-polarizing cocktail in the presence of BMS-986202 (B202). (A) Schematic of polarization protocol. (B) The proportion of FOXP3 + cells was assessed after 7 days. Representative plots and quantification are shown ( n = 9). (C) Expression of RORC2, CCR4, and CCR6 was determined after 7 days relative to non-polarized, control Tregs ( n = 3). (D) Methylation of the Treg-specific demethylated region <t>(TSDR)</t> after 7 days of culture ( n = 5). (E) Representative plot and quantification of intracellular IL-17A/F expression after a 4 h stimulation with PMA/ionomycin ( n = 5). (F) IL-17A, IL-17F, and TNF-α concentration in supernatant after a 24 h re-stimulation with anti-CD3/CD28 ( n = 6). (G) Representative figures and quantification of TIGIT and CD226 expression on Tregs on day 7 ( n = 9). Statistically significant differences compared to DMSO-treated cells were determined by a repeated-measures one-way ANOVA with Dunnett’s multiple comparisons test (B–G). Bars indicate mean ± SEM. Points indicate individual replicates. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
    Tsdr Pyrosequencing Primer Agaaatttgtggggtggg, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    1) Product Images from "TYK2 inhibition enhances Treg differentiation and function while preventing Th1 and Th17 differentiation"

    Article Title: TYK2 inhibition enhances Treg differentiation and function while preventing Th1 and Th17 differentiation

    Journal: Cell Reports Medicine

    doi: 10.1016/j.xcrm.2025.102303

    TYK2 inhibition enhances Treg stability in Th17-polarizing conditions Tregs (CD4 + CD25 hi CD127 lo ) were isolated from peripheral blood of healthy human donors, stimulated with anti-CD3/CD28, and cultured for 7 days in a Th17-polarizing cocktail in the presence of BMS-986202 (B202). (A) Schematic of polarization protocol. (B) The proportion of FOXP3 + cells was assessed after 7 days. Representative plots and quantification are shown ( n = 9). (C) Expression of RORC2, CCR4, and CCR6 was determined after 7 days relative to non-polarized, control Tregs ( n = 3). (D) Methylation of the Treg-specific demethylated region (TSDR) after 7 days of culture ( n = 5). (E) Representative plot and quantification of intracellular IL-17A/F expression after a 4 h stimulation with PMA/ionomycin ( n = 5). (F) IL-17A, IL-17F, and TNF-α concentration in supernatant after a 24 h re-stimulation with anti-CD3/CD28 ( n = 6). (G) Representative figures and quantification of TIGIT and CD226 expression on Tregs on day 7 ( n = 9). Statistically significant differences compared to DMSO-treated cells were determined by a repeated-measures one-way ANOVA with Dunnett’s multiple comparisons test (B–G). Bars indicate mean ± SEM. Points indicate individual replicates. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
    Figure Legend Snippet: TYK2 inhibition enhances Treg stability in Th17-polarizing conditions Tregs (CD4 + CD25 hi CD127 lo ) were isolated from peripheral blood of healthy human donors, stimulated with anti-CD3/CD28, and cultured for 7 days in a Th17-polarizing cocktail in the presence of BMS-986202 (B202). (A) Schematic of polarization protocol. (B) The proportion of FOXP3 + cells was assessed after 7 days. Representative plots and quantification are shown ( n = 9). (C) Expression of RORC2, CCR4, and CCR6 was determined after 7 days relative to non-polarized, control Tregs ( n = 3). (D) Methylation of the Treg-specific demethylated region (TSDR) after 7 days of culture ( n = 5). (E) Representative plot and quantification of intracellular IL-17A/F expression after a 4 h stimulation with PMA/ionomycin ( n = 5). (F) IL-17A, IL-17F, and TNF-α concentration in supernatant after a 24 h re-stimulation with anti-CD3/CD28 ( n = 6). (G) Representative figures and quantification of TIGIT and CD226 expression on Tregs on day 7 ( n = 9). Statistically significant differences compared to DMSO-treated cells were determined by a repeated-measures one-way ANOVA with Dunnett’s multiple comparisons test (B–G). Bars indicate mean ± SEM. Points indicate individual replicates. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

    Techniques Used: Inhibition, Isolation, Cell Culture, Expressing, Control, Methylation, Concentration Assay

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    Purification:

    Article Title: Inter-generational resemblance of methylation levels at circadian genes and associations with phenology in the barn swallow
    Article Snippet: The PCR product was bound to Streptavidin Sepharose HP (Amersham Biosciences, Uppsala, Sweden) and the Sepharose beads containing the immobilized PCR product were purified, washed, denatured using a 0.2 M NaOH solution, and washed again using the Pyrosequencing Vacuum Prep Tool (Pyrosequencing, Inc., Westborough, MA), as recommended by the manufacturer. .. Then, 0.3 μΜ pyrosequencing primer was annealed to the purified single-stranded PCR product and pyrosequencing was performed using the PyroMark MD System (Pyrosequencing, Inc.). ..

    Article Title: Blood DNA methylation, nevi number and the risk of melanoma.
    Article Snippet: The PCR product was bound to Streptavidin Sepharose HP (Amersham Biosciences, Uppsala, Sweden) and the Sepharose beads containing the immobilized PCR product were purified, washed, denatured using a 0.2 M NaOH solution, and washed again using the Pyrosequencing Vacuum Prep Tool (Pyrosequencing, Inc., Westborough, MA), as recommended by the manufacturer. .. Then, 0.3 μM pyrosequencing primer was annealed to the purified single-stranded PCR product and pyrosequencing was performed using the PyroMark MD System (Pyrosequencing, Inc.). ..

    Polymerase Chain Reaction:

    Article Title: Inter-generational resemblance of methylation levels at circadian genes and associations with phenology in the barn swallow
    Article Snippet: The PCR product was bound to Streptavidin Sepharose HP (Amersham Biosciences, Uppsala, Sweden) and the Sepharose beads containing the immobilized PCR product were purified, washed, denatured using a 0.2 M NaOH solution, and washed again using the Pyrosequencing Vacuum Prep Tool (Pyrosequencing, Inc., Westborough, MA), as recommended by the manufacturer. .. Then, 0.3 μΜ pyrosequencing primer was annealed to the purified single-stranded PCR product and pyrosequencing was performed using the PyroMark MD System (Pyrosequencing, Inc.). ..

    Article Title: Blood DNA methylation, nevi number and the risk of melanoma.
    Article Snippet: The PCR product was bound to Streptavidin Sepharose HP (Amersham Biosciences, Uppsala, Sweden) and the Sepharose beads containing the immobilized PCR product were purified, washed, denatured using a 0.2 M NaOH solution, and washed again using the Pyrosequencing Vacuum Prep Tool (Pyrosequencing, Inc., Westborough, MA), as recommended by the manufacturer. .. Then, 0.3 μM pyrosequencing primer was annealed to the purified single-stranded PCR product and pyrosequencing was performed using the PyroMark MD System (Pyrosequencing, Inc.). ..

    other:

    Article Title: Night Shift Work, DNA Methylation and Telomere Length: An Investigation on Hospital Female Nurses
    Article Snippet: Pyrosequencing primer (0.3 μΜ) was annealed to the purified single-stranded PCR products, and pyrosequencing was performed with the PyroMark MD System (Pyrosequencing, Inc. Westborough, MA, USA).

    Article Title: Role of Dietary Factors on DNA Methylation Levels of TNF-Alpha Gene and Proteome Profiles in Obese Men
    Article Snippet: Pyrosequencing primer (0.3 μM) was annealed to the purified single-stranded PCR product, and pyrosequencing was performed with the PyroMark MD System (Pyrosequencing, Inc.).

    Article Title: A circular RNA map for human induced pluripotent stem cells of foetal origin
    Article Snippet: Pyrosequencing primer (0.3 μM) was annealed to the purified single-stranded PCR product in presence of 15 μL of annealing buffer, during an incubation of 2 min at 85 °C.

    Article Title: Targeted resequencing of FECH locus reveals that a novel deep intronic pathogenic variant and eQTLs may cause erythropoietic protoporphyria (EPP) through a methylation-dependent mechanism.
    Article Snippet: Then, pyrosequencing primer (0.3 μm) was annealed to the purified single-stranded PCR product, and methylation analysis was performed by PyroMark MD Q96 (Pyrosequencing, Inc. Westborough, MA).



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    TYK2 inhibition enhances Treg stability in Th17-polarizing conditions Tregs (CD4 + CD25 hi CD127 lo ) were isolated from peripheral blood of healthy human donors, stimulated with anti-CD3/CD28, and cultured for 7 days in a Th17-polarizing cocktail in the presence of BMS-986202 (B202). (A) Schematic of polarization protocol. (B) The proportion of FOXP3 + cells was assessed after 7 days. Representative plots and quantification are shown ( n = 9). (C) Expression of RORC2, CCR4, and CCR6 was determined after 7 days relative to non-polarized, control Tregs ( n = 3). (D) Methylation of the Treg-specific demethylated region (TSDR) after 7 days of culture ( n = 5). (E) Representative plot and quantification of intracellular IL-17A/F expression after a 4 h stimulation with PMA/ionomycin ( n = 5). (F) IL-17A, IL-17F, and TNF-α concentration in supernatant after a 24 h re-stimulation with anti-CD3/CD28 ( n = 6). (G) Representative figures and quantification of TIGIT and CD226 expression on Tregs on day 7 ( n = 9). Statistically significant differences compared to DMSO-treated cells were determined by a repeated-measures one-way ANOVA with Dunnett’s multiple comparisons test (B–G). Bars indicate mean ± SEM. Points indicate individual replicates. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

    Journal: Cell Reports Medicine

    Article Title: TYK2 inhibition enhances Treg differentiation and function while preventing Th1 and Th17 differentiation

    doi: 10.1016/j.xcrm.2025.102303

    Figure Lengend Snippet: TYK2 inhibition enhances Treg stability in Th17-polarizing conditions Tregs (CD4 + CD25 hi CD127 lo ) were isolated from peripheral blood of healthy human donors, stimulated with anti-CD3/CD28, and cultured for 7 days in a Th17-polarizing cocktail in the presence of BMS-986202 (B202). (A) Schematic of polarization protocol. (B) The proportion of FOXP3 + cells was assessed after 7 days. Representative plots and quantification are shown ( n = 9). (C) Expression of RORC2, CCR4, and CCR6 was determined after 7 days relative to non-polarized, control Tregs ( n = 3). (D) Methylation of the Treg-specific demethylated region (TSDR) after 7 days of culture ( n = 5). (E) Representative plot and quantification of intracellular IL-17A/F expression after a 4 h stimulation with PMA/ionomycin ( n = 5). (F) IL-17A, IL-17F, and TNF-α concentration in supernatant after a 24 h re-stimulation with anti-CD3/CD28 ( n = 6). (G) Representative figures and quantification of TIGIT and CD226 expression on Tregs on day 7 ( n = 9). Statistically significant differences compared to DMSO-treated cells were determined by a repeated-measures one-way ANOVA with Dunnett’s multiple comparisons test (B–G). Bars indicate mean ± SEM. Points indicate individual replicates. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

    Article Snippet: TSDR Pyrosequencing primer: AGAAATTTGTGGGGTGGG , Boardman et al. , N/A.

    Techniques: Inhibition, Isolation, Cell Culture, Expressing, Control, Methylation, Concentration Assay

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: The exocyst subunit EXOC2 regulates the toxicity of expanded GGGGCC repeats in C9ORF72 -ALS/FTD

    doi: 10.1016/j.celrep.2024.114375

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: C9ORF72-V3 pyrosequencing primers, See , Yuva-Aydemir et al. , N/A.

    Techniques: Reverse Transcription, Enzyme-linked Immunosorbent Assay, Control, Recombinant, Plasmid Preparation, Software

    Overview of the data and methods used in this study. a , b , c , d depict the primary analyses, in which a latent variable model was used to determine the association between peripheral TPH2 and SLC6A4 methylation and brain levels of 5-HTT and 5-HT 4 . e , f , g describe the sensitivity analyses evaluating the association between SLC6A4 / TPH2 methylation and measures of environmental stress, depressive and anxiety symptoms. h and i show sensitivity analyses used to evaluate potential influence of blood cell proportions in the A-G analyses. Abbreviations: 5-HT 4 : serotonin 4 receptor; 5-HTT: serotonin transporter; CpG: CpG site; TSS: transcription start site; SLE: stressful life events; PBI: parental bonding inventory; BDI: Beck’s depressive index; GAD10: generalized anxiety disorder 10-item; CATS: childhood abuse trauma scale; HAMD6: Hamilton depressive rating scale 6; PSS: perceived stress scale; CpG LV : latent variable including all CpG methylation values

    Journal: Clinical Epigenetics

    Article Title: No association between peripheral serotonin-gene-related DNA methylation and brain serotonin neurotransmission in the healthy and depressed state

    doi: 10.1186/s13148-024-01678-y

    Figure Lengend Snippet: Overview of the data and methods used in this study. a , b , c , d depict the primary analyses, in which a latent variable model was used to determine the association between peripheral TPH2 and SLC6A4 methylation and brain levels of 5-HTT and 5-HT 4 . e , f , g describe the sensitivity analyses evaluating the association between SLC6A4 / TPH2 methylation and measures of environmental stress, depressive and anxiety symptoms. h and i show sensitivity analyses used to evaluate potential influence of blood cell proportions in the A-G analyses. Abbreviations: 5-HT 4 : serotonin 4 receptor; 5-HTT: serotonin transporter; CpG: CpG site; TSS: transcription start site; SLE: stressful life events; PBI: parental bonding inventory; BDI: Beck’s depressive index; GAD10: generalized anxiety disorder 10-item; CATS: childhood abuse trauma scale; HAMD6: Hamilton depressive rating scale 6; PSS: perceived stress scale; CpG LV : latent variable including all CpG methylation values

    Article Snippet: Primers used for pyrosequencing SLC6A4 and TPH2 (XLSX 9 kb) Table S4.

    Techniques: Methylation, CpG Methylation Assay

    Demographics of the participants included in the primary analyses

    Journal: Clinical Epigenetics

    Article Title: No association between peripheral serotonin-gene-related DNA methylation and brain serotonin neurotransmission in the healthy and depressed state

    doi: 10.1186/s13148-024-01678-y

    Figure Lengend Snippet: Demographics of the participants included in the primary analyses

    Article Snippet: Primers used for pyrosequencing SLC6A4 and TPH2 (XLSX 9 kb) Table S4.

    Techniques: Methylation

    Associations between peripheral TPH2 DNA methylation and brain 5-HTT binding a or 5-HT 4 binding b in the healthy cohort. Orange dashed boxes to the left depict the TPH2 CpG sites and the covariates included in the model. Rs45706210 stands for TPH2 rs45706210 G/T SNP. For representation purposes, PET and MR scanner covariates are not reported in the a and b models. Similarly, although included in the 5-HT 4 latent variable model, 5-HTTLPR/rs25531 and BDNF rs6265 genotypes are not reported in ( b ). Scatter plots in c and d depict the relation between TPH2 methylation and 5-HTT LV or 5-HT 4LV in healthy controls ( c , d ), while the relation between TPH2 methylation and 5-HT 4 binding in patients with MDD is showed in ( e )

    Journal: Clinical Epigenetics

    Article Title: No association between peripheral serotonin-gene-related DNA methylation and brain serotonin neurotransmission in the healthy and depressed state

    doi: 10.1186/s13148-024-01678-y

    Figure Lengend Snippet: Associations between peripheral TPH2 DNA methylation and brain 5-HTT binding a or 5-HT 4 binding b in the healthy cohort. Orange dashed boxes to the left depict the TPH2 CpG sites and the covariates included in the model. Rs45706210 stands for TPH2 rs45706210 G/T SNP. For representation purposes, PET and MR scanner covariates are not reported in the a and b models. Similarly, although included in the 5-HT 4 latent variable model, 5-HTTLPR/rs25531 and BDNF rs6265 genotypes are not reported in ( b ). Scatter plots in c and d depict the relation between TPH2 methylation and 5-HTT LV or 5-HT 4LV in healthy controls ( c , d ), while the relation between TPH2 methylation and 5-HT 4 binding in patients with MDD is showed in ( e )

    Article Snippet: Primers used for pyrosequencing SLC6A4 and TPH2 (XLSX 9 kb) Table S4.

    Techniques: DNA Methylation Assay, Binding Assay, Methylation

    Results of latent variable models (LVM) evaluating the association between SLC6A4 / TPH2 methylation and 5-HTT and 5-HT 4 brain binding

    Journal: Clinical Epigenetics

    Article Title: No association between peripheral serotonin-gene-related DNA methylation and brain serotonin neurotransmission in the healthy and depressed state

    doi: 10.1186/s13148-024-01678-y

    Figure Lengend Snippet: Results of latent variable models (LVM) evaluating the association between SLC6A4 / TPH2 methylation and 5-HTT and 5-HT 4 brain binding

    Article Snippet: Primers used for pyrosequencing SLC6A4 and TPH2 (XLSX 9 kb) Table S4.

    Techniques: Methylation

    Association between a latent variable including blood cell proportions (CpG LV+cells ) and a latent variable including serotonin transporter (5-HTT) or serotonin 4 receptor (5-HT 4 ) binding

    Journal: Clinical Epigenetics

    Article Title: No association between peripheral serotonin-gene-related DNA methylation and brain serotonin neurotransmission in the healthy and depressed state

    doi: 10.1186/s13148-024-01678-y

    Figure Lengend Snippet: Association between a latent variable including blood cell proportions (CpG LV+cells ) and a latent variable including serotonin transporter (5-HTT) or serotonin 4 receptor (5-HT 4 ) binding

    Article Snippet: Primers used for pyrosequencing SLC6A4 and TPH2 (XLSX 9 kb) Table S4.

    Techniques:

    Methodology of studies assessing the gut microbiota composition in cats with suspected or confirmed chronic enteropathies.

    Journal: Frontiers in Microbiology

    Article Title: Gut microbiota in cats with inflammatory bowel disease and low-grade intestinal T-cell lymphoma

    doi: 10.3389/fmicb.2024.1346639

    Figure Lengend Snippet: Methodology of studies assessing the gut microbiota composition in cats with suspected or confirmed chronic enteropathies.

    Article Snippet: , 15 colony cats with uncharacterized chronic diarrhea , Feces , 16S rRNA gene pyrosequencing Primers for the V1-V2 region.

    Techniques: Sequencing, Fluorescence, In Situ Hybridization, Real-time Polymerase Chain Reaction, Bacteria