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crispr cas9 genomic engineering idt star methods rbns primers idt star methods recombinant dna px458 addgene plasmid  (Addgene inc)


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    Addgene inc crispr cas9 genomic engineering idt star methods rbns primers idt star methods recombinant dna px458 addgene plasmid
    Crispr Cas9 Genomic Engineering Idt Star Methods Rbns Primers Idt Star Methods Recombinant Dna Px458 Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4083 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px458/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm41932309-792-83-97
    Average 96 stars, based on 4083 article reviews
    crispr cas9 genomic engineering idt star methods rbns primers idt star methods recombinant dna px458 addgene plasmid - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Ribosome Molecular Aging Shapes Translation Dynamics
    Article Snippet: .. The guide RNA, GAACCGCCACCATGGTAAGG, was designed with CRISPOR and cloned into a modified version of PX458 (Addgene, Plasmid #48138) with a Cas9-mCherry fusion (gift from the Daniel Larson Lab, NCI). ..

    Article Title: The conserved N-terminal SANT1-binding domain (SBD) of EZH2 regulates PRC2 activity.
    Article Snippet: .. Plasmid generation and lentivirus production for cell culture Single guide RNAs (sgRNAs) directed againstmouse Ezh2 were cloned into PX458 (Addgene 48138; a gift from F. Zhang). .. Mouse Ezh2 cDNA sequences from Horizon Dharmacon were cloned into PiggyBac (pCAGGS-IRESNeo; a gift fromH.

    Article Title: Plagl1 and Lrrc58 control mammalian body size by triggering target-directed microRNA degradation of miR-322 and miR-503
    Article Snippet: .. MEF trigger site knockout clones were generated in one of two ways: (1) Guide sequences were cloned into px458 (Addgene 48138) ( ) expressing GFP and Cas9, and immortalized MEFs were transfected with plasmids using FuGENE HD (Promega). .. Twenty-four hours after transfection, GFP-positive cells were sorted into 96 well plates using a Melody cell sorter (BD Biosciences), and clonal cell lines were established. (2) sgRNAs were synthesized (Integrated DNA Technologies), resuspended at a final concentration of 25 μM, and incubated with 25 μM Alt-R S.p.

    Article Title: Combined targeted and epigenetic-based therapy enhances antitumor immunity by stabilizing GATA6-dependent MHCI expression in pancreatic ductal adenocarcinoma
    Article Snippet: HAs were PCR-amplified using murine primary PDAC cell line (110299) genomic DNA as template and cloned into pJET (Thermo Fisher Scientific) flanking the Blast-P2A-V5-AID cassette to generate pJET_gata6_N-AID_HDR. .. Two sgRNAs targeting around the gata6 start codon were cloned into PX458 (Addgene #48138) to obtain PX458_gata6_sgRNA1 and PX458_ gata6_sgRNA2. ..

    Modification:

    Article Title: Ribosome Molecular Aging Shapes Translation Dynamics
    Article Snippet: .. The guide RNA, GAACCGCCACCATGGTAAGG, was designed with CRISPOR and cloned into a modified version of PX458 (Addgene, Plasmid #48138) with a Cas9-mCherry fusion (gift from the Daniel Larson Lab, NCI). ..

    Plasmid Preparation:

    Article Title: Ribosome Molecular Aging Shapes Translation Dynamics
    Article Snippet: .. The guide RNA, GAACCGCCACCATGGTAAGG, was designed with CRISPOR and cloned into a modified version of PX458 (Addgene, Plasmid #48138) with a Cas9-mCherry fusion (gift from the Daniel Larson Lab, NCI). ..

    Article Title: The conserved N-terminal SANT1-binding domain (SBD) of EZH2 regulates PRC2 activity.
    Article Snippet: .. Plasmid generation and lentivirus production for cell culture Single guide RNAs (sgRNAs) directed againstmouse Ezh2 were cloned into PX458 (Addgene 48138; a gift from F. Zhang). .. Mouse Ezh2 cDNA sequences from Horizon Dharmacon were cloned into PiggyBac (pCAGGS-IRESNeo; a gift fromH.

    Article Title: U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAs
    Article Snippet: Recombinant DNA , , . .. PX458 , Addgene , Plasmid #48138. .. U2AF1 C-terminal 3XFlag Donor plasmid , This study , Addgene Plasmid.

    Article Title: Generation of functionally competent testicular somatic cells from pluripotent stem cells.
    Article Snippet: After cleavage at Kpn I and Sac I sites to replace the Rosa homology arm of the pDonor- Rosa26 plasmid (Addgene; no. 37200) and Sox92A- CGFP, each cloned DNA fragment was joined together using an In- Fusion HD cloning kit (Takara Bio, Shiga, Japan) to generate the Sox9- CGFP targeting vector. .. A Cas9 and chimeric guide RNA expression plasmid, pX458 (pSpCas9- 2A- GFP) (Addgene; no. 48138), which expresses Cas9 nuclease, D ow nloaded from https://w w w .science.org on M arch 01, 2026 Sato et al., Sci. ..

    Cell Culture:

    Article Title: The conserved N-terminal SANT1-binding domain (SBD) of EZH2 regulates PRC2 activity.
    Article Snippet: .. Plasmid generation and lentivirus production for cell culture Single guide RNAs (sgRNAs) directed againstmouse Ezh2 were cloned into PX458 (Addgene 48138; a gift from F. Zhang). .. Mouse Ezh2 cDNA sequences from Horizon Dharmacon were cloned into PiggyBac (pCAGGS-IRESNeo; a gift fromH.

    Knock-Out:

    Article Title: The integrated stress response promotes immune evasion through lipocalin 2.
    Article Snippet: Jozef P. Bossowski, Ray Pillai, John Kilian, Angela Wong Lau, Mari Nakamura, Ali Rashidfarrokhi, Yuan Hao, Ruxuan Li, Katherine Wu, Takamitsu Hattori, Eliezra Glasser, Akiko Koide, Lidong Wang, Andre L. Moreira, Cristina Hajdu, Sahith Rajalingam, Sarah E. LeBoeuf, Hortense Le, Seungeun Lee, Jin Woo Oh, Cheolyong Joe, Hyemin Kim, Chan-Young Ock, Se-Hoon Lee, Hao Wang, Angana A. H. Patel, Volkan I. Sayin, Aristotelis Tsirigos, Kwok-Kin Wong, Sergei B. Koralov, Mario Pende, Francisco J. Sánchez-Rivera, Diane M. Simeone, Ioannis K. Zervantonakis, Shohei Koide4,9 ✉ & Thales Papagiannakopoulos1,4 ✉

    Article Title: Plagl1 and Lrrc58 control mammalian body size by triggering target-directed microRNA degradation of miR-322 and miR-503
    Article Snippet: .. MEF trigger site knockout clones were generated in one of two ways: (1) Guide sequences were cloned into px458 (Addgene 48138) ( ) expressing GFP and Cas9, and immortalized MEFs were transfected with plasmids using FuGENE HD (Promega). .. Twenty-four hours after transfection, GFP-positive cells were sorted into 96 well plates using a Melody cell sorter (BD Biosciences), and clonal cell lines were established. (2) sgRNAs were synthesized (Integrated DNA Technologies), resuspended at a final concentration of 25 μM, and incubated with 25 μM Alt-R S.p.

    Generated:

    Article Title: The integrated stress response promotes immune evasion through lipocalin 2.
    Article Snippet: Jozef P. Bossowski, Ray Pillai, John Kilian, Angela Wong Lau, Mari Nakamura, Ali Rashidfarrokhi, Yuan Hao, Ruxuan Li, Katherine Wu, Takamitsu Hattori, Eliezra Glasser, Akiko Koide, Lidong Wang, Andre L. Moreira, Cristina Hajdu, Sahith Rajalingam, Sarah E. LeBoeuf, Hortense Le, Seungeun Lee, Jin Woo Oh, Cheolyong Joe, Hyemin Kim, Chan-Young Ock, Se-Hoon Lee, Hao Wang, Angana A. H. Patel, Volkan I. Sayin, Aristotelis Tsirigos, Kwok-Kin Wong, Sergei B. Koralov, Mario Pende, Francisco J. Sánchez-Rivera, Diane M. Simeone, Ioannis K. Zervantonakis, Shohei Koide4,9 ✉ & Thales Papagiannakopoulos1,4 ✉

    Article Title: Plagl1 and Lrrc58 control mammalian body size by triggering target-directed microRNA degradation of miR-322 and miR-503
    Article Snippet: .. MEF trigger site knockout clones were generated in one of two ways: (1) Guide sequences were cloned into px458 (Addgene 48138) ( ) expressing GFP and Cas9, and immortalized MEFs were transfected with plasmids using FuGENE HD (Promega). .. Twenty-four hours after transfection, GFP-positive cells were sorted into 96 well plates using a Melody cell sorter (BD Biosciences), and clonal cell lines were established. (2) sgRNAs were synthesized (Integrated DNA Technologies), resuspended at a final concentration of 25 μM, and incubated with 25 μM Alt-R S.p.

    Transfection:

    Article Title: The integrated stress response promotes immune evasion through lipocalin 2.
    Article Snippet: Jozef P. Bossowski, Ray Pillai, John Kilian, Angela Wong Lau, Mari Nakamura, Ali Rashidfarrokhi, Yuan Hao, Ruxuan Li, Katherine Wu, Takamitsu Hattori, Eliezra Glasser, Akiko Koide, Lidong Wang, Andre L. Moreira, Cristina Hajdu, Sahith Rajalingam, Sarah E. LeBoeuf, Hortense Le, Seungeun Lee, Jin Woo Oh, Cheolyong Joe, Hyemin Kim, Chan-Young Ock, Se-Hoon Lee, Hao Wang, Angana A. H. Patel, Volkan I. Sayin, Aristotelis Tsirigos, Kwok-Kin Wong, Sergei B. Koralov, Mario Pende, Francisco J. Sánchez-Rivera, Diane M. Simeone, Ioannis K. Zervantonakis, Shohei Koide4,9 ✉ & Thales Papagiannakopoulos1,4 ✉

    Article Title: Plagl1 and Lrrc58 control mammalian body size by triggering target-directed microRNA degradation of miR-322 and miR-503
    Article Snippet: .. MEF trigger site knockout clones were generated in one of two ways: (1) Guide sequences were cloned into px458 (Addgene 48138) ( ) expressing GFP and Cas9, and immortalized MEFs were transfected with plasmids using FuGENE HD (Promega). .. Twenty-four hours after transfection, GFP-positive cells were sorted into 96 well plates using a Melody cell sorter (BD Biosciences), and clonal cell lines were established. (2) sgRNAs were synthesized (Integrated DNA Technologies), resuspended at a final concentration of 25 μM, and incubated with 25 μM Alt-R S.p.

    Expressing:

    Article Title: The integrated stress response promotes immune evasion through lipocalin 2.
    Article Snippet: Jozef P. Bossowski, Ray Pillai, John Kilian, Angela Wong Lau, Mari Nakamura, Ali Rashidfarrokhi, Yuan Hao, Ruxuan Li, Katherine Wu, Takamitsu Hattori, Eliezra Glasser, Akiko Koide, Lidong Wang, Andre L. Moreira, Cristina Hajdu, Sahith Rajalingam, Sarah E. LeBoeuf, Hortense Le, Seungeun Lee, Jin Woo Oh, Cheolyong Joe, Hyemin Kim, Chan-Young Ock, Se-Hoon Lee, Hao Wang, Angana A. H. Patel, Volkan I. Sayin, Aristotelis Tsirigos, Kwok-Kin Wong, Sergei B. Koralov, Mario Pende, Francisco J. Sánchez-Rivera, Diane M. Simeone, Ioannis K. Zervantonakis, Shohei Koide4,9 ✉ & Thales Papagiannakopoulos1,4 ✉

    Article Title: Plagl1 and Lrrc58 control mammalian body size by triggering target-directed microRNA degradation of miR-322 and miR-503
    Article Snippet: .. MEF trigger site knockout clones were generated in one of two ways: (1) Guide sequences were cloned into px458 (Addgene 48138) ( ) expressing GFP and Cas9, and immortalized MEFs were transfected with plasmids using FuGENE HD (Promega). .. Twenty-four hours after transfection, GFP-positive cells were sorted into 96 well plates using a Melody cell sorter (BD Biosciences), and clonal cell lines were established. (2) sgRNAs were synthesized (Integrated DNA Technologies), resuspended at a final concentration of 25 μM, and incubated with 25 μM Alt-R S.p.

    RNA Expression:

    Article Title: Generation of functionally competent testicular somatic cells from pluripotent stem cells.
    Article Snippet: After cleavage at Kpn I and Sac I sites to replace the Rosa homology arm of the pDonor- Rosa26 plasmid (Addgene; no. 37200) and Sox92A- CGFP, each cloned DNA fragment was joined together using an In- Fusion HD cloning kit (Takara Bio, Shiga, Japan) to generate the Sox9- CGFP targeting vector. .. A Cas9 and chimeric guide RNA expression plasmid, pX458 (pSpCas9- 2A- GFP) (Addgene; no. 48138), which expresses Cas9 nuclease, D ow nloaded from https://w w w .science.org on M arch 01, 2026 Sato et al., Sci. ..

    Purification:

    Article Title: Acute Degradation of Pumilio Proteins Uncovers a Biphasic Post-transcriptional Regulatory Hierarchy Controlling Embryonic Stem Cell Fate Decisions
    Article Snippet: .. The PX458 (addgene #48138) or PX459 (addgene #62988) vector was linearized with BbsI-HF digestion and gel purified. ..



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