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pwpxl empty vector  (Addgene inc)


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    Structured Review

    Addgene inc pwpxl empty vector
    Pwpxl Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pwpxl+vector/pWPXL+(Plasmid+%2312257)/pm40752636-81-16-18
    Average 94 stars, based on 153 article reviews
    pwpxl empty vector - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Sequencing:

    Article Title: Identification and characterization of a carbohydrate recognition domain-like region in Entamoeba histolytica Gal/GalNAc lectin intermediate subunit
    Article Snippet: In brief, as full-length Igl (GenBank accession no. AF337950.1 ) without the SP and GPI-anchored sequences, Igl–F and its segments were amplified and subcloned into pET-19b vectors (Novagen, Darmstadt, Germany) within the XhoI restriction site. .. After obtaining the EGFP sequence from a pWPXL vector (Addgene, #12257), FLAG–HA–pcDNA3.1- vectors (Addgene, #52535) were used for EGFP–Igl–C3 expression on the CHO cell surface. ..

    Article Title: The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics.
    Article Snippet: TMEM182, a transmembrane protein highly expressed in muscle and adipose tissues, plays a crucial role in muscle cell differentiation, metabolism, and signaling.. However, its role in fat deposition and metabolism is still unknown.. In this study, we used overexpression and knockout models to examine the impact of TMEM182 on fat synthesis and metabolism.

    Article Title: Identification and characterization of a carbohydrate recognition domain-like region in Entamoeba histolytica Gal/GalNAc lectin intermediate subunit.
    Article Snippet: In brief, as fulllength Igl (GenBank accession no. AF337950.1) without the SP and GPI-anchored sequences, Igl–F and its segments were amplified and subcloned into pET-19b vec tors (Novagen, Darmstadt, Germany) within the XhoI restriction site. .. After obtaining the EGFP sequence from a pWPXL vector (Addgene, #12257), FLAG–HA–pcDNA3.1vectors (Addgene, #52535) were used for EGFP–Igl–C3 expression on the CHO cell surface. ..

    Expressing:

    Article Title: Identification and characterization of a carbohydrate recognition domain-like region in Entamoeba histolytica Gal/GalNAc lectin intermediate subunit
    Article Snippet: In brief, as full-length Igl (GenBank accession no. AF337950.1 ) without the SP and GPI-anchored sequences, Igl–F and its segments were amplified and subcloned into pET-19b vectors (Novagen, Darmstadt, Germany) within the XhoI restriction site. .. After obtaining the EGFP sequence from a pWPXL vector (Addgene, #12257), FLAG–HA–pcDNA3.1- vectors (Addgene, #52535) were used for EGFP–Igl–C3 expression on the CHO cell surface. ..

    Article Title: Identification and characterization of a carbohydrate recognition domain-like region in Entamoeba histolytica Gal/GalNAc lectin intermediate subunit.
    Article Snippet: In brief, as fulllength Igl (GenBank accession no. AF337950.1) without the SP and GPI-anchored sequences, Igl–F and its segments were amplified and subcloned into pET-19b vec tors (Novagen, Darmstadt, Germany) within the XhoI restriction site. .. After obtaining the EGFP sequence from a pWPXL vector (Addgene, #12257), FLAG–HA–pcDNA3.1vectors (Addgene, #52535) were used for EGFP–Igl–C3 expression on the CHO cell surface. ..

    Over Expression:

    Article Title: The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics.
    Article Snippet: TMEM182, a transmembrane protein highly expressed in muscle and adipose tissues, plays a crucial role in muscle cell differentiation, metabolism, and signaling.. However, its role in fat deposition and metabolism is still unknown.. In this study, we used overexpression and knockout models to examine the impact of TMEM182 on fat synthesis and metabolism.

    Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer.
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in Table S5. .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in . .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer
    Article Snippet: Rapid ampli cation of cDNA ends (RACE) We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC97L cells using the SMARTer RACE cDNA Ampli cation Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol. .. Vector construction and transfection of cell lines The full-length of tsTE1 was ampli ed from the cRNA of MHCC-97L cells as stated in Rapid ampli cation of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in Supplementary Table 5. .. The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing.

    Amplification:

    Article Title: The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics.
    Article Snippet: TMEM182, a transmembrane protein highly expressed in muscle and adipose tissues, plays a crucial role in muscle cell differentiation, metabolism, and signaling.. However, its role in fat deposition and metabolism is still unknown.. In this study, we used overexpression and knockout models to examine the impact of TMEM182 on fat synthesis and metabolism.

    Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer.
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in Table S5. .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in . .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    Clone Assay:

    Article Title: The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics.
    Article Snippet: TMEM182, a transmembrane protein highly expressed in muscle and adipose tissues, plays a crucial role in muscle cell differentiation, metabolism, and signaling.. However, its role in fat deposition and metabolism is still unknown.. In this study, we used overexpression and knockout models to examine the impact of TMEM182 on fat synthesis and metabolism.

    Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer.
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in Table S5. .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Modular Cell Line for Scalable and Rapid In Vitro Evaluation of Chimeric Antigen Receptors
    Article Snippet: .. They were cloned into the pWPXL vector (#12257, Addgene) under the control of the EF-1a promoter. ..

    Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in . .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer
    Article Snippet: Rapid ampli cation of cDNA ends (RACE) We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC97L cells using the SMARTer RACE cDNA Ampli cation Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol. .. Vector construction and transfection of cell lines The full-length of tsTE1 was ampli ed from the cRNA of MHCC-97L cells as stated in Rapid ampli cation of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in Supplementary Table 5. .. The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing.

    Article Title: Modular cell line for scalable and rapid in vitro evaluation of chimeric antigen receptors.
    Article Snippet: .. They were cloned into the pWPXL vector (#12257, Addgene) under the control of the EF1α promoter. ..

    Plasmid Preparation:

    Article Title: The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics.
    Article Snippet: TMEM182, a transmembrane protein highly expressed in muscle and adipose tissues, plays a crucial role in muscle cell differentiation, metabolism, and signaling.. However, its role in fat deposition and metabolism is still unknown.. In this study, we used overexpression and knockout models to examine the impact of TMEM182 on fat synthesis and metabolism.

    Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer.
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in Table S5. .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Modular Cell Line for Scalable and Rapid In Vitro Evaluation of Chimeric Antigen Receptors
    Article Snippet: .. They were cloned into the pWPXL vector (#12257, Addgene) under the control of the EF-1a promoter. ..

    Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in . .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer
    Article Snippet: Rapid ampli cation of cDNA ends (RACE) We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC97L cells using the SMARTer RACE cDNA Ampli cation Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol. .. Vector construction and transfection of cell lines The full-length of tsTE1 was ampli ed from the cRNA of MHCC-97L cells as stated in Rapid ampli cation of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in Supplementary Table 5. .. The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing.

    Article Title: Modular cell line for scalable and rapid in vitro evaluation of chimeric antigen receptors.
    Article Snippet: .. They were cloned into the pWPXL vector (#12257, Addgene) under the control of the EF1α promoter. ..

    Transfection:

    Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer.
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in Table S5. .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer
    Article Snippet: Rapid ampli cation of cDNA ends (RACE) We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC97L cells using the SMARTer RACE cDNA Ampli cation Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol. .. Vector construction and transfection of cell lines The full-length of tsTE1 was ampli ed from the cRNA of MHCC-97L cells as stated in Rapid ampli cation of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in Supplementary Table 5. .. The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing.

    Rapid Amplification of cDNA Ends:

    Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer.
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in Table S5. .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in . .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    In Vitro:

    Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer.
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in Table S5. .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in . .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer
    Article Snippet: Rapid ampli cation of cDNA ends (RACE) We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC97L cells using the SMARTer RACE cDNA Ampli cation Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol. .. Vector construction and transfection of cell lines The full-length of tsTE1 was ampli ed from the cRNA of MHCC-97L cells as stated in Rapid ampli cation of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in Supplementary Table 5. .. The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing.

    Synthesized:

    Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer.
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in Table S5. .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer
    Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in . .. Cells were transfected at a final concentration of 20 nM using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s instructions.

    Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer
    Article Snippet: Rapid ampli cation of cDNA ends (RACE) We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC97L cells using the SMARTer RACE cDNA Ampli cation Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol. .. Vector construction and transfection of cell lines The full-length of tsTE1 was ampli ed from the cRNA of MHCC-97L cells as stated in Rapid ampli cation of cDNA ends (RACE) subsection, and then cloned into pWPXL vector (Addgene #12257) generating pwpxl-tsTE1 plasmid for overexpression assay in vitro. siRNAs were synthesized by Ribobio (Guangzhou, China) and the sequences are provided in Supplementary Table 5. .. The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing.

    Control:

    Article Title: Modular Cell Line for Scalable and Rapid In Vitro Evaluation of Chimeric Antigen Receptors
    Article Snippet: .. They were cloned into the pWPXL vector (#12257, Addgene) under the control of the EF-1a promoter. ..

    Article Title: Modular cell line for scalable and rapid in vitro evaluation of chimeric antigen receptors.
    Article Snippet: .. They were cloned into the pWPXL vector (#12257, Addgene) under the control of the EF1α promoter. ..



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    Image Search Results


    Fig. 3. Schematic representation of pWPXL-aNab-Short and pWPXL-aNab-Long vectors (a); alfa-GFP stained cells (b); Flow cytometry analysis of transduced K-562 cells (c) before and (d) after selection step with alfa-GFP staining; The dynamics of alfa-GFP signal during cultivation of K-562, K-562-Short, and K-562-Long cells (f); The dynamics of MFI values during incubation for K-562-Long or K-562-Short cells (e).

    Journal: Protein expression and purification

    Article Title: Modular cell line for scalable and rapid in vitro evaluation of chimeric antigen receptors.

    doi: 10.1016/j.pep.2025.106738

    Figure Lengend Snippet: Fig. 3. Schematic representation of pWPXL-aNab-Short and pWPXL-aNab-Long vectors (a); alfa-GFP stained cells (b); Flow cytometry analysis of transduced K-562 cells (c) before and (d) after selection step with alfa-GFP staining; The dynamics of alfa-GFP signal during cultivation of K-562, K-562-Short, and K-562-Long cells (f); The dynamics of MFI values during incubation for K-562-Long or K-562-Short cells (e).

    Article Snippet: The assembly of constructs was performed in the pWPXL lentiviral vector (#12257, Addgene) under the constant EF-1a promoter, followed by the components described previously.

    Techniques: Staining, Flow Cytometry, Selection, Incubation