pwpxl empty vector (Addgene inc)
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Pwpxl Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pwpxl+vector/pWPXL+(Plasmid+%2312257)/pm40752636-81-16-18
Average 94 stars, based on 153 article reviews
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Sequencing:Article Title: Identification and characterization of a carbohydrate recognition domain-like region in Entamoeba histolytica Gal/GalNAc lectin intermediate subunit Article Snippet: In brief, as full-length Igl (GenBank accession no. AF337950.1 ) without the SP and GPI-anchored sequences, Igl–F and its segments were amplified and subcloned into pET-19b vectors (Novagen, Darmstadt, Germany) within the XhoI restriction site. .. After obtaining the EGFP sequence from a Article Title: The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics. Article Snippet: TMEM182, a transmembrane protein highly expressed in muscle and adipose tissues, plays a crucial role in muscle cell differentiation, metabolism, and signaling.. However, its role in fat deposition and metabolism is still unknown.. In this study, we used overexpression and knockout models to examine the impact of TMEM182 on fat synthesis and metabolism. Article Title: Identification and characterization of a carbohydrate recognition domain-like region in Entamoeba histolytica Gal/GalNAc lectin intermediate subunit. Article Snippet: In brief, as fulllength Igl (GenBank accession no. AF337950.1) without the SP and GPI-anchored sequences, Igl–F and its segments were amplified and subcloned into pET-19b vec tors (Novagen, Darmstadt, Germany) within the XhoI restriction site. .. After obtaining the EGFP sequence from a Expressing:Article Title: Identification and characterization of a carbohydrate recognition domain-like region in Entamoeba histolytica Gal/GalNAc lectin intermediate subunit Article Snippet: In brief, as full-length Igl (GenBank accession no. AF337950.1 ) without the SP and GPI-anchored sequences, Igl–F and its segments were amplified and subcloned into pET-19b vectors (Novagen, Darmstadt, Germany) within the XhoI restriction site. .. After obtaining the EGFP sequence from a Article Title: Identification and characterization of a carbohydrate recognition domain-like region in Entamoeba histolytica Gal/GalNAc lectin intermediate subunit. Article Snippet: In brief, as fulllength Igl (GenBank accession no. AF337950.1) without the SP and GPI-anchored sequences, Igl–F and its segments were amplified and subcloned into pET-19b vec tors (Novagen, Darmstadt, Germany) within the XhoI restriction site. .. After obtaining the EGFP sequence from a Over Expression:Article Title: The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics. Article Snippet: TMEM182, a transmembrane protein highly expressed in muscle and adipose tissues, plays a crucial role in muscle cell differentiation, metabolism, and signaling.. However, its role in fat deposition and metabolism is still unknown.. In this study, we used overexpression and knockout models to examine the impact of TMEM182 on fat synthesis and metabolism. Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer. Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer Article Snippet: Rapid ampli cation of cDNA ends (RACE) We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC97L cells using the SMARTer RACE cDNA Ampli cation Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol. .. Vector construction and transfection of cell lines The full-length of tsTE1 was ampli ed from the cRNA of MHCC-97L cells as stated in Rapid ampli cation of cDNA ends (RACE) subsection, and then cloned into Amplification:Article Title: The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics. Article Snippet: TMEM182, a transmembrane protein highly expressed in muscle and adipose tissues, plays a crucial role in muscle cell differentiation, metabolism, and signaling.. However, its role in fat deposition and metabolism is still unknown.. In this study, we used overexpression and knockout models to examine the impact of TMEM182 on fat synthesis and metabolism. Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer. Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into Clone Assay:Article Title: The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics. Article Snippet: TMEM182, a transmembrane protein highly expressed in muscle and adipose tissues, plays a crucial role in muscle cell differentiation, metabolism, and signaling.. However, its role in fat deposition and metabolism is still unknown.. In this study, we used overexpression and knockout models to examine the impact of TMEM182 on fat synthesis and metabolism. Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer. Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into Article Title: Modular Cell Line for Scalable and Rapid In Vitro Evaluation of Chimeric Antigen Receptors Article Snippet: .. They were cloned into the Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer Article Snippet: Rapid ampli cation of cDNA ends (RACE) We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC97L cells using the SMARTer RACE cDNA Ampli cation Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol. .. Vector construction and transfection of cell lines The full-length of tsTE1 was ampli ed from the cRNA of MHCC-97L cells as stated in Rapid ampli cation of cDNA ends (RACE) subsection, and then cloned into Article Title: Modular cell line for scalable and rapid in vitro evaluation of chimeric antigen receptors. Article Snippet: .. They were cloned into the Plasmid Preparation:Article Title: The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics. Article Snippet: TMEM182, a transmembrane protein highly expressed in muscle and adipose tissues, plays a crucial role in muscle cell differentiation, metabolism, and signaling.. However, its role in fat deposition and metabolism is still unknown.. In this study, we used overexpression and knockout models to examine the impact of TMEM182 on fat synthesis and metabolism. Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer. Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into Article Title: Modular Cell Line for Scalable and Rapid In Vitro Evaluation of Chimeric Antigen Receptors Article Snippet: .. They were cloned into the Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer Article Snippet: Rapid ampli cation of cDNA ends (RACE) We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC97L cells using the SMARTer RACE cDNA Ampli cation Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol. .. Vector construction and transfection of cell lines The full-length of tsTE1 was ampli ed from the cRNA of MHCC-97L cells as stated in Rapid ampli cation of cDNA ends (RACE) subsection, and then cloned into Article Title: Modular cell line for scalable and rapid in vitro evaluation of chimeric antigen receptors. Article Snippet: .. They were cloned into the Transfection:Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer. Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer Article Snippet: Rapid ampli cation of cDNA ends (RACE) We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC97L cells using the SMARTer RACE cDNA Ampli cation Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol. .. Vector construction and transfection of cell lines The full-length of tsTE1 was ampli ed from the cRNA of MHCC-97L cells as stated in Rapid ampli cation of cDNA ends (RACE) subsection, and then cloned into Rapid Amplification of cDNA Ends:Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer. Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into In Vitro:Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer. Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer Article Snippet: Rapid ampli cation of cDNA ends (RACE) We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC97L cells using the SMARTer RACE cDNA Ampli cation Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol. .. Vector construction and transfection of cell lines The full-length of tsTE1 was ampli ed from the cRNA of MHCC-97L cells as stated in Rapid ampli cation of cDNA ends (RACE) subsection, and then cloned into Synthesized:Article Title: RNA splicing junction landscape reveals abundant tumor-specific transcripts in human cancer. Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. Vector construction and transfection of cell lines The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer Article Snippet: The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing. .. The full-length of tsTE1 was amplified from the cRNA of MHCC-97L cells as stated in Rapid amplification of cDNA ends (RACE) subsection, and then cloned into Article Title: RNA Splicing Junction Landscape Reveals Abundant Tumor-Specific Transcripts in Human Cancer Article Snippet: Rapid ampli cation of cDNA ends (RACE) We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC97L cells using the SMARTer RACE cDNA Ampli cation Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol. .. Vector construction and transfection of cell lines The full-length of tsTE1 was ampli ed from the cRNA of MHCC-97L cells as stated in Rapid ampli cation of cDNA ends (RACE) subsection, and then cloned into Control:Article Title: Modular Cell Line for Scalable and Rapid In Vitro Evaluation of Chimeric Antigen Receptors Article Snippet: .. They were cloned into the Article Title: Modular cell line for scalable and rapid in vitro evaluation of chimeric antigen receptors. Article Snippet: .. They were cloned into the |
