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pvrc8400 vector  (Addgene inc)


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    Structured Review

    Addgene inc pvrc8400 vector
    KEY RESOURCES TABLE
    Pvrc8400 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pvrc8400+vector/WNV+VLPs+pVRC8400+(Plasmid+%2363160)/pmc11979902-77-0-3
    Average 93 stars, based on 22 article reviews
    pvrc8400 vector - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class"

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.115163

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Virus, Recombinant, Staining, Peptide Microarray, Transfection, Reverse Transcription, Reporter Gene Assay, Plasmid Preparation, Expressing, Software

    Related Articles

    Plasmid Preparation:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .

    Virus:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .

    Recombinant:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .

    Staining:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .

    Peptide Microarray:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .

    Transfection:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .

    Reverse Transcription:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .

    Reporter Gene Assay:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .

    Expressing:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .

    Software:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .

    Mutagenesis:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .

    Sequencing:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .

    Neutralization:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .

    Enzyme-linked Immunosorbent Assay:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .

    Modification:

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.CMVR rhesus IgG expression plasmid , NIH/VRC , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.Rhesus Macaque Igl expression vector , NIH/VRC, Mason et al. , VRC3952.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. Software and algorithms , , .Software and algorithms , , .

    Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , Corrigan et al. , N/A.pVRC8400-RM19R plasmid , Corrigan et al. , N/A.

    Article Title: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
    Article Snippet: Recombinant DNA , , .Recombinant DNA , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-VRC40.01 plasmid , This paper , N/A.pVRC8400-VRC40.01 plasmid , This paper , N/A.

    Article Title: Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain
    Article Snippet: pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.pLEXm , Laboratory of Daniel Leahy, Johns Hopkins University, USA , N/A.. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. gWiz-blank , Aldevron , Cat# 5009.gWiz-blank , Aldevron , Cat# 5009.

    Article Title: Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat# 63160.. pVRC8400-RM19R plasmid , This paper , N/A.pVRC8400-RM19R plasmid , This paper , N/A.

    Article Title: Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class
    Article Snippet: , , .. pVRC8400 vector , https://www.addgene.org , Cat#63160; RRID:Addgene_63164.. gWiz , Aldeveron , Cat# 5008.gWiz , Aldeveron , Cat# 5008.

    Article Title: Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite
    Article Snippet: pα-H vector , Laboratory of Daniel Leahy , N/A.pα-H vector , Laboratory of Daniel Leahy , N/A.. pVRC8400 vector , https://www.addgene.org , Cat#63160.. , , .



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class

    doi: 10.1016/j.celrep.2024.115163

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: pVRC8400 vector , https://www.addgene.org , Cat# 63160.

    Techniques: Virus, Recombinant, Staining, Peptide Microarray, Transfection, Reverse Transcription, Reporter Gene Assay, Plasmid Preparation, Expressing, Software

    Journal: Structure (London, England : 1993)

    Article Title: Vaccine-elicited murine antibody WS6 neutralizes diverse beta-coronaviruses by recognizing a helical stem supersite of vulnerability

    doi: 10.1016/j.str.2022.06.004

    Figure Lengend Snippet:

    Article Snippet: The heavy and light chain variable region sequences were synthesized with codon optimized for human cell expression and then subcloned into corresponding pVRC8400 vectors containing mouse IgG1 heavy chain and kappa light chain constant regions by GenScript (Piscataway, NJ).

    Techniques: Virus, Recombinant, Luciferase, Transfection, Peptide Microarray, Cloning, Mutagenesis, Software