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pvivo2  (InvivoGen)


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    Structured Review

    InvivoGen pvivo2
    Pvivo2, supplied by InvivoGen, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pvivo2/pVIVO2-mcs/pmc11784016-273-1-2
    Average 93 stars, based on 24 article reviews
    pvivo2 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Single Cell:

    Article Title: Variability in HIV-1 Transmitted/Founder Virus Susceptibility to Combined APOBEC3F and APOBEC3G Host Restriction
    Article Snippet: .. The pVIVO2 (Invivogen) has two MCS to enable co-expression of two genes on a single-cell basis. .. GeneJuice (EMD Millipore) transfection reagent was used according to manufacturer’s instructions.

    Article Title: Variability in HIV-1 transmitted/founder virus susceptibility to combined APOBEC3F and APOBEC3G host restriction.
    Article Snippet: Transfections used 500 ng of an HIV-1 infectious molecular clone (LAI, LAI SLQM, T/F CH058, T/F CH470, T/F CH040, T/F CH077, T/F Thro, T/F CH236, T/F CH264, T/F CH850, or T/F CH569) and 25 or 50 ng of pVIVO2 containing no A3 (empty vector), A3G-HA, A3F-V5, or both A3F-V5 and A3G-HA. .. The pVIVO2 (Invivogen) has two MCSs to enable co-expression of two genes on a single-cell basis. .. GeneJuice (Novagen) transfection reagent was used according to manufacturer’s instructions.

    Article Title: APOBEC3D excludes APOBEC3F from HIV-1 virions by competitive binding of RNA
    Article Snippet: .. To express two A3 transcripts on a single-cell basis, we used an expression plasmid, pVIVO2 (Invivogen), with two transcription units in a single vector. ..

    Article Title: Variability in HIV-1 transmitted/founder virus susceptibility to combined APOBEC3F and APOBEC3G host restriction
    Article Snippet: Transfections used 500 ng of an HIV-1 infectious molecular clone (LAI, LAI SLQ M , T/F CH058, T/F CH470, T/F CH040, T/F CH077, T/F Thro, T/F CH236, T/F CH264, T/F CH850, or T/F CH569) and 25 or 50 ng of pVIVO2 containing no A3 (empty vector), A3G-HA, A3F-V5, or both A3F-V5 and A3G-HA. .. The pVIVO2 (Invivogen) has two MCSs to enable co-expression of two genes on a single-cell basis. .. GeneJuice (Novagen) transfection reagent was used according to manufacturer’s instructions.

    Expressing:

    Article Title: Stability of APOBEC3F in the Presence of the APOBEC3 Antagonist HIV-1 Vif Increases at the Expense of Co-Expressed APOBEC3H Haplotype I
    Article Snippet: .. To express two A3 transcripts on a single-cell basis, we used an expression plasmid, pVIVO2 (Invivogen, San Diego, CA, USA), with two transcription units in a single vector. ..

    Article Title: Stability of APOBEC3F in the Presence of the APOBEC3 Antagonist HIV-1 Vif Increases at the Expense of Co-Expressed APOBEC3H Haplotype I.
    Article Snippet: .. To express two A3 transcripts on a single-cell basis, we used an expression plasmid, pVIVO2 (Invivogen, San Diego, CA, USA), with two transcription units in a single vector. ..

    Article Title: APOBEC3D excludes APOBEC3F from HIV-1 virions by competitive binding of RNA
    Article Snippet: .. To express two A3 transcripts on a single-cell basis, we used an expression plasmid, pVIVO2 (Invivogen), with two transcription units in a single vector. ..

    Plasmid Preparation:

    Article Title: Stability of APOBEC3F in the Presence of the APOBEC3 Antagonist HIV-1 Vif Increases at the Expense of Co-Expressed APOBEC3H Haplotype I
    Article Snippet: .. To express two A3 transcripts on a single-cell basis, we used an expression plasmid, pVIVO2 (Invivogen, San Diego, CA, USA), with two transcription units in a single vector. ..

    Article Title: MMP-7 derived peptides with MHC class-I binding motifs from canine mammary tumor tissue elicit strong antigen-specific T-cell responses in BALB/c mice
    Article Snippet: .. MMP-7 gene from canine mammary tumor and murine MMP-7 was cloned in pVIVO2-mcs vector (InvivoGen, USA) for customizing xenogenic MMP-7 DNA vaccine constructs. ..

    Article Title: Role of co-expressed APOBEC3F and APOBEC3G in inducing HIV-1 drug resistance
    Article Snippet: .. HIV-1 LAI ΔVif ΔEnv or HIV-1 LAI ΔEnv (500 ng) were cotransfected into 1 × 10 5 293T cells with 25, 50, or 100 ng of A3G in MCS-II of pVIVO2 (Invivogen) vector with a C-terminal HA tag, A3F in MCS-I of pVIVO2 vector with a C-terminal V5 tag, or A3F/A3G where A3F was cloned into MCS-I of pVIVO2 with a C-terminal V5 tag and A3G was cloned into the MCS-II of the pVIVO2 vector with a C-terminal HA tag. ..

    Article Title: Stability of APOBEC3F in the Presence of the APOBEC3 Antagonist HIV-1 Vif Increases at the Expense of Co-Expressed APOBEC3H Haplotype I.
    Article Snippet: .. To express two A3 transcripts on a single-cell basis, we used an expression plasmid, pVIVO2 (Invivogen, San Diego, CA, USA), with two transcription units in a single vector. ..

    Article Title: APOBEC3D excludes APOBEC3F from HIV-1 virions by competitive binding of RNA
    Article Snippet: .. To express two A3 transcripts on a single-cell basis, we used an expression plasmid, pVIVO2 (Invivogen), with two transcription units in a single vector. ..

    Clone Assay:

    Article Title: MMP-7 derived peptides with MHC class-I binding motifs from canine mammary tumor tissue elicit strong antigen-specific T-cell responses in BALB/c mice
    Article Snippet: .. MMP-7 gene from canine mammary tumor and murine MMP-7 was cloned in pVIVO2-mcs vector (InvivoGen, USA) for customizing xenogenic MMP-7 DNA vaccine constructs. ..

    Article Title: Role of co-expressed APOBEC3F and APOBEC3G in inducing HIV-1 drug resistance
    Article Snippet: .. HIV-1 LAI ΔVif ΔEnv or HIV-1 LAI ΔEnv (500 ng) were cotransfected into 1 × 10 5 293T cells with 25, 50, or 100 ng of A3G in MCS-II of pVIVO2 (Invivogen) vector with a C-terminal HA tag, A3F in MCS-I of pVIVO2 vector with a C-terminal V5 tag, or A3F/A3G where A3F was cloned into MCS-I of pVIVO2 with a C-terminal V5 tag and A3G was cloned into the MCS-II of the pVIVO2 vector with a C-terminal HA tag. ..

    Construct:

    Article Title: MMP-7 derived peptides with MHC class-I binding motifs from canine mammary tumor tissue elicit strong antigen-specific T-cell responses in BALB/c mice
    Article Snippet: .. MMP-7 gene from canine mammary tumor and murine MMP-7 was cloned in pVIVO2-mcs vector (InvivoGen, USA) for customizing xenogenic MMP-7 DNA vaccine constructs. ..



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