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purification large  (TaKaRa)


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    TaKaRa purification large
    Purification Large, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/purification+large/Capturem+His-Tagged+Purification+Large+Volume/pm40255451-61-8-12
    Average 99 stars, based on 21 article reviews
    purification large - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Disruption of extracellular redox balance drives persistent lung fibrosis and impairs fibrosis resolution.
    Article Snippet: Lung fibrosis is a devastating outcome of various diffuse parenchymal lung diseases.. Despite rigorous research efforts, the mechanisms that propagate its progressive and nonresolving nature remain enigmatic.. Oxidative stress has been implicated in the pathogenesis of lung fibrosis.

    Article Title: Genetically engineered silk fibroin with antibacterial and antioxidant properties.
    Article Snippet: Bombyx mori silk fibroin and the antimicrobial peptide cecropin B are promising biomolecules for biomedical applications due to their unique and complementary properties.. In this study, we successfully expressed recombinant silk fibroin (R. fib) alone and in fusion with cecropin B (R. fib–cec) in Pichia pastoris, with yields of 6 mg/L for R. fib and 1.3 mg/L for the R. fib–cec fusion protein.. Characterization of the proteins through SDS-PAGE, Western blotting, and MALDI-TOF confirmed the successful expression and purity of the recombinant proteins.

    Cell Culture:

    Article Title: Disruption of extracellular redox balance drives persistent lung fibrosis and impairs fibrosis resolution.
    Article Snippet: Lung fibrosis is a devastating outcome of various diffuse parenchymal lung diseases.. Despite rigorous research efforts, the mechanisms that propagate its progressive and nonresolving nature remain enigmatic.. Oxidative stress has been implicated in the pathogenesis of lung fibrosis.

    Recombinant:

    Article Title: Genetically engineered silk fibroin with antibacterial and antioxidant properties.
    Article Snippet: Bombyx mori silk fibroin and the antimicrobial peptide cecropin B are promising biomolecules for biomedical applications due to their unique and complementary properties.. In this study, we successfully expressed recombinant silk fibroin (R. fib) alone and in fusion with cecropin B (R. fib–cec) in Pichia pastoris, with yields of 6 mg/L for R. fib and 1.3 mg/L for the R. fib–cec fusion protein.. Characterization of the proteins through SDS-PAGE, Western blotting, and MALDI-TOF confirmed the successful expression and purity of the recombinant proteins.



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    (A) Bulk RNA-seq experimental conditions. Control organoids (n = 6 organoids, three differentiations) and L1342P organoids (n = 6 organoids, two differentiations) were sequenced using the AVITI system. (B) Hierarchical clustering of cortical organoid sample replicates. Darker blue indicates higher similarity. (C) Principal Component Analysis (PCA) plot illustrating the clustering of replicates based on their variance in two dimensions. (D) DEG Heatmap showing the top 50 down (blue) and upregulated (red) genes across conditions. (E) Volcano plot highlighting significantly differentially expressed genes (DEGs, FDR < 0.05) in Nav1.2-L1342P hiPSC-derived cortical organoids compared to controls. Notable downregulated genes include SCN2A and potassium channel-related genes. In contrast, upregulated genes include: DLX2 (Distal-Less Homeobox 2), H2AX (histone family member X), PAX6 (Paired Box 6), and ASCL1 (Achaete-scute family bHLH transcription factor 1), which are important in neurodevelopment, neuronal function, and cellular function. (F) Gene Ontology (GO) analysis of biological processes reveals changes in synapse organization, microtubule-cytoskeleton arrangements, glutamate receptor signaling, and forebrain development (top). (G) GO molecular function analysis reveals enrichment in ion channel activity and alterations in the glutamatergic pathway. (H) GO cellular component identifies synaptic pathway alterations in the Nav1.2-L1342P cortical organoids. (I) Network analysis reveals numerous globally differentially expressed genes (DEGs) involved in synaptic signal regulation (magenta), calcium transport (dark green), synapse organization (orange), and forebrain development (lime). The colored edges connecting genes represent functional relationships or interactions within each cluster, highlighting how these DEGs collectively contribute to altered neuronal physiology. (J) REACTOME pathway analysis reveals disruptions in cell division, metabolism, AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptor trafficking, and neuronal synapses. (K) KEGG pathway enrichment analysis reveals alterations in MAPK and cAMP signaling pathways, as well as glutamatergic synapses. The circle size is represented by the number of DEGs highly ranked by p.adjust values.

    Journal: bioRxiv

    Article Title: Epilepsy-Associated SCN2A-L1342P Mutation Drives Network Hyperexcitability and Widespread Transcriptomic Changes in Human Cortical Organoids

    doi: 10.1101/2025.08.18.670956

    Figure Lengend Snippet: (A) Bulk RNA-seq experimental conditions. Control organoids (n = 6 organoids, three differentiations) and L1342P organoids (n = 6 organoids, two differentiations) were sequenced using the AVITI system. (B) Hierarchical clustering of cortical organoid sample replicates. Darker blue indicates higher similarity. (C) Principal Component Analysis (PCA) plot illustrating the clustering of replicates based on their variance in two dimensions. (D) DEG Heatmap showing the top 50 down (blue) and upregulated (red) genes across conditions. (E) Volcano plot highlighting significantly differentially expressed genes (DEGs, FDR < 0.05) in Nav1.2-L1342P hiPSC-derived cortical organoids compared to controls. Notable downregulated genes include SCN2A and potassium channel-related genes. In contrast, upregulated genes include: DLX2 (Distal-Less Homeobox 2), H2AX (histone family member X), PAX6 (Paired Box 6), and ASCL1 (Achaete-scute family bHLH transcription factor 1), which are important in neurodevelopment, neuronal function, and cellular function. (F) Gene Ontology (GO) analysis of biological processes reveals changes in synapse organization, microtubule-cytoskeleton arrangements, glutamate receptor signaling, and forebrain development (top). (G) GO molecular function analysis reveals enrichment in ion channel activity and alterations in the glutamatergic pathway. (H) GO cellular component identifies synaptic pathway alterations in the Nav1.2-L1342P cortical organoids. (I) Network analysis reveals numerous globally differentially expressed genes (DEGs) involved in synaptic signal regulation (magenta), calcium transport (dark green), synapse organization (orange), and forebrain development (lime). The colored edges connecting genes represent functional relationships or interactions within each cluster, highlighting how these DEGs collectively contribute to altered neuronal physiology. (J) REACTOME pathway analysis reveals disruptions in cell division, metabolism, AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptor trafficking, and neuronal synapses. (K) KEGG pathway enrichment analysis reveals alterations in MAPK and cAMP signaling pathways, as well as glutamatergic synapses. The circle size is represented by the number of DEGs highly ranked by p.adjust values.

    Article Snippet: To this end, we extracted total RNA from the cortical organoids using the NucleoSpin miRNA Kit for the Isolation of Small and large RNA (Macherey-Nagel, Catalog No. 740971.50) according to the manufacturer’s instructions.

    Techniques: RNA Sequencing, Control, Derivative Assay, Cell Function Assay, Activity Assay, Functional Assay, Protein-Protein interactions