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aav php eb capsid  (Addgene inc)


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    Structured Review

    Addgene inc aav php eb capsid
    Aav Php Eb Capsid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pucmini+icap+php+eb/pUCmini-iCAP-PHP%2EeB+(Plasmid+%23103005)/pm42875809-80-10-12
    Average 97 stars, based on 207 article reviews
    aav php eb capsid - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Characterizing stroke-related cellular changes in the surviving neurons of mouse ischemic stroke.
    Article Snippet: The A-CREB plasmid (Addgene #33371, Watertown, MA, USA) was subcloned into the hSyn1-eYFP vector (Addgene #117382). .. AAV 293 cells were co-transfected with pUCmini-iCAP-PHP.eB (Addgene #103005), rAAV-ITR CREB, CREB S133A, A-CREB expression plasmid (single-stranded genome), pAAV-GFP control plasmid (Cell Biolabs Inc.), or hSyn1-eYFP vector, along with the helper plasmid (Agilent Technologies, Santa Clara, CA, USA), using the calcium phosphate precipitation method. ..

    Article Title: Blockade of associative spine enlargement impairs awake cortical function
    Article Snippet: .. Briefly, plasmids encoding the AAV vector, pHelper (Stratagene), and pUCmini-iCAP-PHP.eB (Addgene #103005) were transfected into HEK293 (AAV293) cells (Stratagene). ..

    Article Title: Cell-type-specific repression reveals MC4R neurons as a site of action for ciliary obesity genes.
    Article Snippet: For each virus, three 15-cm dishes were seeded with 1.5×107 AAVpro cells (Takara, 632273, passage number less than 10) in 40 mL complete DMEM [DMEM (Gibco, 11995065) supplemented with 10% FBS (Gibco, A5670801) and 1% penicillin–streptomycin (Sigma-Aldrich, P4333)]. .. The following day, cells were transfected with a DNA–PEI mixture containing 20 μg pAdDeltaF6 (Addgene #112867; gift from James M. Wilson), 11 μg pUCmini-iCAP-PHP.eB (Addgene #103005; gift from Viviana Gradinaru [91]), 7 μg of the AAV sgRNA plasmid, and 75 μL of 1 μg/μL linear PEI (Polysciences, 23966) in 4 mL Opti-MEM (Gibco, 31985062). ..

    Expressing:

    Article Title: Characterizing stroke-related cellular changes in the surviving neurons of mouse ischemic stroke.
    Article Snippet: The A-CREB plasmid (Addgene #33371, Watertown, MA, USA) was subcloned into the hSyn1-eYFP vector (Addgene #117382). .. AAV 293 cells were co-transfected with pUCmini-iCAP-PHP.eB (Addgene #103005), rAAV-ITR CREB, CREB S133A, A-CREB expression plasmid (single-stranded genome), pAAV-GFP control plasmid (Cell Biolabs Inc.), or hSyn1-eYFP vector, along with the helper plasmid (Agilent Technologies, Santa Clara, CA, USA), using the calcium phosphate precipitation method. ..

    Plasmid Preparation:

    Article Title: Characterizing stroke-related cellular changes in the surviving neurons of mouse ischemic stroke.
    Article Snippet: The A-CREB plasmid (Addgene #33371, Watertown, MA, USA) was subcloned into the hSyn1-eYFP vector (Addgene #117382). .. AAV 293 cells were co-transfected with pUCmini-iCAP-PHP.eB (Addgene #103005), rAAV-ITR CREB, CREB S133A, A-CREB expression plasmid (single-stranded genome), pAAV-GFP control plasmid (Cell Biolabs Inc.), or hSyn1-eYFP vector, along with the helper plasmid (Agilent Technologies, Santa Clara, CA, USA), using the calcium phosphate precipitation method. ..

    Article Title: δ2-Protocadherins organize parallel indirect basal ganglia circuits
    Article Snippet: .. AAVpro 293T cells (Takara Bio) were transfected with pAAV-hSyn-EGFP-CAAX, pAdDeltaF6 (Addgene; Plasmid #112867), and pUCmini-iCAP-PHP.eB (Addgene; Plasmid #103005) using polyethylenimine. ..

    Article Title: Blockade of associative spine enlargement impairs awake cortical function
    Article Snippet: .. Briefly, plasmids encoding the AAV vector, pHelper (Stratagene), and pUCmini-iCAP-PHP.eB (Addgene #103005) were transfected into HEK293 (AAV293) cells (Stratagene). ..

    Article Title: Cell-type-specific repression reveals MC4R neurons as a site of action for ciliary obesity genes.
    Article Snippet: For each virus, three 15-cm dishes were seeded with 1.5×107 AAVpro cells (Takara, 632273, passage number less than 10) in 40 mL complete DMEM [DMEM (Gibco, 11995065) supplemented with 10% FBS (Gibco, A5670801) and 1% penicillin–streptomycin (Sigma-Aldrich, P4333)]. .. The following day, cells were transfected with a DNA–PEI mixture containing 20 μg pAdDeltaF6 (Addgene #112867; gift from James M. Wilson), 11 μg pUCmini-iCAP-PHP.eB (Addgene #103005; gift from Viviana Gradinaru [91]), 7 μg of the AAV sgRNA plasmid, and 75 μL of 1 μg/μL linear PEI (Polysciences, 23966) in 4 mL Opti-MEM (Gibco, 31985062). ..

    Article Title: EvoPRAISE: computationally guided directed evolution for rational AAV capsid engineering
    Article Snippet: .. The mutated capsid fragments were amplified using primers flanking the mutagenized region and cloned into the corresponding site of the pUCmini-iCAP-PHP.eB (Addgene plasmid #103005). ..

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome.
    Article Snippet: © The Author(s) 2026.. Published by Oxford University Press.. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Article Title: Computationally guided discovery of Ly6e/LY6E-dependent AAV capsid variants
    Article Snippet: .. The mutated capsid 524 fragments were amplified using primers flanking the mutagenized region and cloned into the 525 corresponding site of the pUCmini-iCAP-PHP.eB (Addgene plasmid #103005). ..

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome
    Article Snippet: We re-amplified the rat Slc9a6 cDNA from the TOPO vector and cloned into pAAV-L7–6-GFP-WPRE [ ] (Addgene, Plasmid #126462, gifted from Hirokazu Hirai) to generate pAAV-L7-6-Slc9a6-GFP. .. We generated recombinant AAVs in the University of Utah Drug Discovery Core Facility by co-transfecting HEK-293 T cells with either pAAV/L7–6-Slc9a6-GFP or the control pAAV-L7–6-GFP, in addition to pHelper (Stratagene, La Jolla, CA, USA) and pUCmini-iCAP-PHP.eB [ ] (Addgene, plasmid #103005, gifted from Viviana Gradinaru), followed by viral particles purification, concentration, and genomic titer determination. ..

    Control:

    Article Title: Characterizing stroke-related cellular changes in the surviving neurons of mouse ischemic stroke.
    Article Snippet: The A-CREB plasmid (Addgene #33371, Watertown, MA, USA) was subcloned into the hSyn1-eYFP vector (Addgene #117382). .. AAV 293 cells were co-transfected with pUCmini-iCAP-PHP.eB (Addgene #103005), rAAV-ITR CREB, CREB S133A, A-CREB expression plasmid (single-stranded genome), pAAV-GFP control plasmid (Cell Biolabs Inc.), or hSyn1-eYFP vector, along with the helper plasmid (Agilent Technologies, Santa Clara, CA, USA), using the calcium phosphate precipitation method. ..

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome.
    Article Snippet: © The Author(s) 2026.. Published by Oxford University Press.. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome
    Article Snippet: We re-amplified the rat Slc9a6 cDNA from the TOPO vector and cloned into pAAV-L7–6-GFP-WPRE [ ] (Addgene, Plasmid #126462, gifted from Hirokazu Hirai) to generate pAAV-L7-6-Slc9a6-GFP. .. We generated recombinant AAVs in the University of Utah Drug Discovery Core Facility by co-transfecting HEK-293 T cells with either pAAV/L7–6-Slc9a6-GFP or the control pAAV-L7–6-GFP, in addition to pHelper (Stratagene, La Jolla, CA, USA) and pUCmini-iCAP-PHP.eB [ ] (Addgene, plasmid #103005, gifted from Viviana Gradinaru), followed by viral particles purification, concentration, and genomic titer determination. ..

    Transfection:

    Article Title: δ2-Protocadherins organize parallel indirect basal ganglia circuits
    Article Snippet: .. AAVpro 293T cells (Takara Bio) were transfected with pAAV-hSyn-EGFP-CAAX, pAdDeltaF6 (Addgene; Plasmid #112867), and pUCmini-iCAP-PHP.eB (Addgene; Plasmid #103005) using polyethylenimine. ..

    Article Title: Blockade of associative spine enlargement impairs awake cortical function
    Article Snippet: .. Briefly, plasmids encoding the AAV vector, pHelper (Stratagene), and pUCmini-iCAP-PHP.eB (Addgene #103005) were transfected into HEK293 (AAV293) cells (Stratagene). ..

    Article Title: Cell-type-specific repression reveals MC4R neurons as a site of action for ciliary obesity genes.
    Article Snippet: For each virus, three 15-cm dishes were seeded with 1.5×107 AAVpro cells (Takara, 632273, passage number less than 10) in 40 mL complete DMEM [DMEM (Gibco, 11995065) supplemented with 10% FBS (Gibco, A5670801) and 1% penicillin–streptomycin (Sigma-Aldrich, P4333)]. .. The following day, cells were transfected with a DNA–PEI mixture containing 20 μg pAdDeltaF6 (Addgene #112867; gift from James M. Wilson), 11 μg pUCmini-iCAP-PHP.eB (Addgene #103005; gift from Viviana Gradinaru [91]), 7 μg of the AAV sgRNA plasmid, and 75 μL of 1 μg/μL linear PEI (Polysciences, 23966) in 4 mL Opti-MEM (Gibco, 31985062). ..

    Amplification:

    Article Title: EvoPRAISE: computationally guided directed evolution for rational AAV capsid engineering
    Article Snippet: .. The mutated capsid fragments were amplified using primers flanking the mutagenized region and cloned into the corresponding site of the pUCmini-iCAP-PHP.eB (Addgene plasmid #103005). ..

    Article Title: Computationally guided discovery of Ly6e/LY6E-dependent AAV capsid variants
    Article Snippet: .. The mutated capsid 524 fragments were amplified using primers flanking the mutagenized region and cloned into the 525 corresponding site of the pUCmini-iCAP-PHP.eB (Addgene plasmid #103005). ..

    Clone Assay:

    Article Title: EvoPRAISE: computationally guided directed evolution for rational AAV capsid engineering
    Article Snippet: .. The mutated capsid fragments were amplified using primers flanking the mutagenized region and cloned into the corresponding site of the pUCmini-iCAP-PHP.eB (Addgene plasmid #103005). ..

    Article Title: Computationally guided discovery of Ly6e/LY6E-dependent AAV capsid variants
    Article Snippet: .. The mutated capsid 524 fragments were amplified using primers flanking the mutagenized region and cloned into the 525 corresponding site of the pUCmini-iCAP-PHP.eB (Addgene plasmid #103005). ..

    Generated:

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome.
    Article Snippet: © The Author(s) 2026.. Published by Oxford University Press.. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome
    Article Snippet: We re-amplified the rat Slc9a6 cDNA from the TOPO vector and cloned into pAAV-L7–6-GFP-WPRE [ ] (Addgene, Plasmid #126462, gifted from Hirokazu Hirai) to generate pAAV-L7-6-Slc9a6-GFP. .. We generated recombinant AAVs in the University of Utah Drug Discovery Core Facility by co-transfecting HEK-293 T cells with either pAAV/L7–6-Slc9a6-GFP or the control pAAV-L7–6-GFP, in addition to pHelper (Stratagene, La Jolla, CA, USA) and pUCmini-iCAP-PHP.eB [ ] (Addgene, plasmid #103005, gifted from Viviana Gradinaru), followed by viral particles purification, concentration, and genomic titer determination. ..

    Recombinant:

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome.
    Article Snippet: © The Author(s) 2026.. Published by Oxford University Press.. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome
    Article Snippet: We re-amplified the rat Slc9a6 cDNA from the TOPO vector and cloned into pAAV-L7–6-GFP-WPRE [ ] (Addgene, Plasmid #126462, gifted from Hirokazu Hirai) to generate pAAV-L7-6-Slc9a6-GFP. .. We generated recombinant AAVs in the University of Utah Drug Discovery Core Facility by co-transfecting HEK-293 T cells with either pAAV/L7–6-Slc9a6-GFP or the control pAAV-L7–6-GFP, in addition to pHelper (Stratagene, La Jolla, CA, USA) and pUCmini-iCAP-PHP.eB [ ] (Addgene, plasmid #103005, gifted from Viviana Gradinaru), followed by viral particles purification, concentration, and genomic titer determination. ..

    Drug discovery:

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome.
    Article Snippet: © The Author(s) 2026.. Published by Oxford University Press.. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome
    Article Snippet: We re-amplified the rat Slc9a6 cDNA from the TOPO vector and cloned into pAAV-L7–6-GFP-WPRE [ ] (Addgene, Plasmid #126462, gifted from Hirokazu Hirai) to generate pAAV-L7-6-Slc9a6-GFP. .. We generated recombinant AAVs in the University of Utah Drug Discovery Core Facility by co-transfecting HEK-293 T cells with either pAAV/L7–6-Slc9a6-GFP or the control pAAV-L7–6-GFP, in addition to pHelper (Stratagene, La Jolla, CA, USA) and pUCmini-iCAP-PHP.eB [ ] (Addgene, plasmid #103005, gifted from Viviana Gradinaru), followed by viral particles purification, concentration, and genomic titer determination. ..

    Purification:

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome.
    Article Snippet: © The Author(s) 2026.. Published by Oxford University Press.. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome
    Article Snippet: We re-amplified the rat Slc9a6 cDNA from the TOPO vector and cloned into pAAV-L7–6-GFP-WPRE [ ] (Addgene, Plasmid #126462, gifted from Hirokazu Hirai) to generate pAAV-L7-6-Slc9a6-GFP. .. We generated recombinant AAVs in the University of Utah Drug Discovery Core Facility by co-transfecting HEK-293 T cells with either pAAV/L7–6-Slc9a6-GFP or the control pAAV-L7–6-GFP, in addition to pHelper (Stratagene, La Jolla, CA, USA) and pUCmini-iCAP-PHP.eB [ ] (Addgene, plasmid #103005, gifted from Viviana Gradinaru), followed by viral particles purification, concentration, and genomic titer determination. ..

    Concentration Assay:

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome.
    Article Snippet: © The Author(s) 2026.. Published by Oxford University Press.. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome
    Article Snippet: We re-amplified the rat Slc9a6 cDNA from the TOPO vector and cloned into pAAV-L7–6-GFP-WPRE [ ] (Addgene, Plasmid #126462, gifted from Hirokazu Hirai) to generate pAAV-L7-6-Slc9a6-GFP. .. We generated recombinant AAVs in the University of Utah Drug Discovery Core Facility by co-transfecting HEK-293 T cells with either pAAV/L7–6-Slc9a6-GFP or the control pAAV-L7–6-GFP, in addition to pHelper (Stratagene, La Jolla, CA, USA) and pUCmini-iCAP-PHP.eB [ ] (Addgene, plasmid #103005, gifted from Viviana Gradinaru), followed by viral particles purification, concentration, and genomic titer determination. ..

    Titer Analysis:

    Article Title: Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome
    Article Snippet: We re-amplified the rat Slc9a6 cDNA from the TOPO vector and cloned into pAAV-L7–6-GFP-WPRE [ ] (Addgene, Plasmid #126462, gifted from Hirokazu Hirai) to generate pAAV-L7-6-Slc9a6-GFP. .. We generated recombinant AAVs in the University of Utah Drug Discovery Core Facility by co-transfecting HEK-293 T cells with either pAAV/L7–6-Slc9a6-GFP or the control pAAV-L7–6-GFP, in addition to pHelper (Stratagene, La Jolla, CA, USA) and pUCmini-iCAP-PHP.eB [ ] (Addgene, plasmid #103005, gifted from Viviana Gradinaru), followed by viral particles purification, concentration, and genomic titer determination. ..



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