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renilla luciferase reporter plasmid ptk-rl  (Promega)

 
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    Structured Review

    Promega renilla luciferase reporter plasmid ptk-rl
    Renilla Luciferase Reporter Plasmid Ptk Rl, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptk-rl+plasmid/pgl3+basic/pm38549229-52-0-7
    Average 90 stars, based on 1 article reviews
    renilla luciferase reporter plasmid ptk-rl - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Mfn2 downregulation in excitotoxicity causes mitochondrial dysfunction and delayed neuronal death
    Article Snippet: Firefly luciferase-based reporter gene was transfected along with a Renilla expression vector (pTK-RL; Promega), and also, where relevant, MEF2-DN expression vector.

    Article Title: Epidermal Growth Factor Promotes Protein Degradation of Epithelial Protein Lost in Neoplasm (EPLIN), a Putative Metastasis Suppressor, during Epithelial-mesenchymal Transition
    Article Snippet: Plasmids and Constructs—GFP-tagged expression plasmid for EPLIN- (pCMV-EPLIN-AC-GFP) and control plasmid pCMV-AC-GFP were purchased from Origene (Rockville, MD).HumanEPLIN- reporterwas obtained fromSwitchGear Genomics (Menlo Park, CA). pTK-RL plasmid was purchased from Promega (Madison, WI).

    Article Title: Epidermal Growth Factor Promotes Protein Degradation of Epithelial Protein Lost in Neoplasm (EPLIN), a Putative Metastasis Suppressor, during Epithelial-mesenchymal Transition
    Article Snippet: Human EPLIN-β reporter was obtained from SwitchGear Genomics (Menlo Park, CA). pTK-RL plasmid was purchased from Promega (Madison, WI).

    Article Title: Hyperactivation of Stat3 in gp130 mutant mice promotes gastric hyperproliferation and desensitizes TGF-beta signaling.
    Article Snippet: The latent transcription factor Stat3 is activated by gp130, the common receptor for the interleukin (IL)-6 cytokine family and other growth factor and cytokine receptors.. Ligand-induced dimerization of gp130 leads to activation of the Stat1, Stat3 and Shp2-Ras-Erk signaling pathways.. Here we assess genetically the contribution of exaggerated Stat3 activation to the phenotype of gp130Y757F/Y757F mice, in which a knock-in mutation disrupts the negative feedback mechanism on gp130-dependent Stat signaling.

    Article Title: Inhibition of serotonin-induced mitogenesis, migration, and ERK MAPK nuclear translocation in vascular smooth muscle cells by atorvastatin.
    Article Snippet: Li M, Liu Y, Dutt P, Fanburg BL, Toksoz D. Inhibition of serotonin-induced mitogenesis, migration, and ERK MAPK nuclear translocation in vascular smooth muscle cells by atorvastatin.. Am J Physiol Lung Cell Mol Physiol 293: L463–L471, 2007.. First published June 1, 2007; doi:10.1152/ajplung.00133.2007.—The HMGCoA reductase inhibitors, statins, have pleiotropic effects which may include interference with the isoprenylation of Ras and Rho small GTPases.

    Article Title: Helicoverpa armigera GATAe transcriptional factor regulates the expression of Bacillus thuringiensis Cry1Ac receptor gene ABCC2 by its interplay with additional transcription factors.
    Article Snippet: Helicoverpa armigera is a worldwide pest that has been efficiently controlled by transgenic plants expressing Bt Cry toxins.. To exert toxicity, Cry toxins bind to different receptors located in larval midgut cells.. Previously, we reported that GATA transcription factor GATAe activates the expression of multiple H. armigera Cry1Ac receptors in different insect cell lines.

    Construct:

    Article Title: Mfn2 downregulation in excitotoxicity causes mitochondrial dysfunction and delayed neuronal death
    Article Snippet: Firefly luciferase-based reporter gene was transfected along with a Renilla expression vector (pTK-RL; Promega), and also, where relevant, MEF2-DN expression vector.

    Article Title: Epidermal Growth Factor Promotes Protein Degradation of Epithelial Protein Lost in Neoplasm (EPLIN), a Putative Metastasis Suppressor, during Epithelial-mesenchymal Transition
    Article Snippet: Plasmids and Constructs—GFP-tagged expression plasmid for EPLIN- (pCMV-EPLIN-AC-GFP) and control plasmid pCMV-AC-GFP were purchased from Origene (Rockville, MD).HumanEPLIN- reporterwas obtained fromSwitchGear Genomics (Menlo Park, CA). pTK-RL plasmid was purchased from Promega (Madison, WI).

    Article Title: Epidermal Growth Factor Promotes Protein Degradation of Epithelial Protein Lost in Neoplasm (EPLIN), a Putative Metastasis Suppressor, during Epithelial-mesenchymal Transition
    Article Snippet: Human EPLIN-β reporter was obtained from SwitchGear Genomics (Menlo Park, CA). pTK-RL plasmid was purchased from Promega (Madison, WI).

    Article Title: Hyperactivation of Stat3 in gp130 mutant mice promotes gastric hyperproliferation and desensitizes TGF-beta signaling.
    Article Snippet: The latent transcription factor Stat3 is activated by gp130, the common receptor for the interleukin (IL)-6 cytokine family and other growth factor and cytokine receptors.. Ligand-induced dimerization of gp130 leads to activation of the Stat1, Stat3 and Shp2-Ras-Erk signaling pathways.. Here we assess genetically the contribution of exaggerated Stat3 activation to the phenotype of gp130Y757F/Y757F mice, in which a knock-in mutation disrupts the negative feedback mechanism on gp130-dependent Stat signaling.

    Article Title: Inhibition of serotonin-induced mitogenesis, migration, and ERK MAPK nuclear translocation in vascular smooth muscle cells by atorvastatin.
    Article Snippet: Li M, Liu Y, Dutt P, Fanburg BL, Toksoz D. Inhibition of serotonin-induced mitogenesis, migration, and ERK MAPK nuclear translocation in vascular smooth muscle cells by atorvastatin.. Am J Physiol Lung Cell Mol Physiol 293: L463–L471, 2007.. First published June 1, 2007; doi:10.1152/ajplung.00133.2007.—The HMGCoA reductase inhibitors, statins, have pleiotropic effects which may include interference with the isoprenylation of Ras and Rho small GTPases.

    Article Title: Helicoverpa armigera GATAe transcriptional factor regulates the expression of Bacillus thuringiensis Cry1Ac receptor gene ABCC2 by its interplay with additional transcription factors.
    Article Snippet: Helicoverpa armigera is a worldwide pest that has been efficiently controlled by transgenic plants expressing Bt Cry toxins.. To exert toxicity, Cry toxins bind to different receptors located in larval midgut cells.. Previously, we reported that GATA transcription factor GATAe activates the expression of multiple H. armigera Cry1Ac receptors in different insect cell lines.

    Expressing:

    Article Title: Mfn2 downregulation in excitotoxicity causes mitochondrial dysfunction and delayed neuronal death
    Article Snippet: Firefly luciferase-based reporter gene was transfected along with a Renilla expression vector (pTK-RL; Promega), and also, where relevant, MEF2-DN expression vector.

    Article Title: Epidermal Growth Factor Promotes Protein Degradation of Epithelial Protein Lost in Neoplasm (EPLIN), a Putative Metastasis Suppressor, during Epithelial-mesenchymal Transition
    Article Snippet: Plasmids and Constructs—GFP-tagged expression plasmid for EPLIN- (pCMV-EPLIN-AC-GFP) and control plasmid pCMV-AC-GFP were purchased from Origene (Rockville, MD).HumanEPLIN- reporterwas obtained fromSwitchGear Genomics (Menlo Park, CA). pTK-RL plasmid was purchased from Promega (Madison, WI).

    Article Title: Epidermal Growth Factor Promotes Protein Degradation of Epithelial Protein Lost in Neoplasm (EPLIN), a Putative Metastasis Suppressor, during Epithelial-mesenchymal Transition
    Article Snippet: Human EPLIN-β reporter was obtained from SwitchGear Genomics (Menlo Park, CA). pTK-RL plasmid was purchased from Promega (Madison, WI).

    Article Title: Hyperactivation of Stat3 in gp130 mutant mice promotes gastric hyperproliferation and desensitizes TGF-beta signaling.
    Article Snippet: The latent transcription factor Stat3 is activated by gp130, the common receptor for the interleukin (IL)-6 cytokine family and other growth factor and cytokine receptors.. Ligand-induced dimerization of gp130 leads to activation of the Stat1, Stat3 and Shp2-Ras-Erk signaling pathways.. Here we assess genetically the contribution of exaggerated Stat3 activation to the phenotype of gp130Y757F/Y757F mice, in which a knock-in mutation disrupts the negative feedback mechanism on gp130-dependent Stat signaling.

    Article Title: Inhibition of serotonin-induced mitogenesis, migration, and ERK MAPK nuclear translocation in vascular smooth muscle cells by atorvastatin.
    Article Snippet: Li M, Liu Y, Dutt P, Fanburg BL, Toksoz D. Inhibition of serotonin-induced mitogenesis, migration, and ERK MAPK nuclear translocation in vascular smooth muscle cells by atorvastatin.. Am J Physiol Lung Cell Mol Physiol 293: L463–L471, 2007.. First published June 1, 2007; doi:10.1152/ajplung.00133.2007.—The HMGCoA reductase inhibitors, statins, have pleiotropic effects which may include interference with the isoprenylation of Ras and Rho small GTPases.

    Article Title: Helicoverpa armigera GATAe transcriptional factor regulates the expression of Bacillus thuringiensis Cry1Ac receptor gene ABCC2 by its interplay with additional transcription factors.
    Article Snippet: Helicoverpa armigera is a worldwide pest that has been efficiently controlled by transgenic plants expressing Bt Cry toxins.. To exert toxicity, Cry toxins bind to different receptors located in larval midgut cells.. Previously, we reported that GATA transcription factor GATAe activates the expression of multiple H. armigera Cry1Ac receptors in different insect cell lines.

    Luciferase:

    Article Title: Mfn2 downregulation in excitotoxicity causes mitochondrial dysfunction and delayed neuronal death
    Article Snippet: Firefly luciferase-based reporter gene was transfected along with a Renilla expression vector (pTK-RL; Promega), and also, where relevant, MEF2-DN expression vector.

    Article Title: Epidermal Growth Factor Promotes Protein Degradation of Epithelial Protein Lost in Neoplasm (EPLIN), a Putative Metastasis Suppressor, during Epithelial-mesenchymal Transition
    Article Snippet: Plasmids and Constructs—GFP-tagged expression plasmid for EPLIN- (pCMV-EPLIN-AC-GFP) and control plasmid pCMV-AC-GFP were purchased from Origene (Rockville, MD).HumanEPLIN- reporterwas obtained fromSwitchGear Genomics (Menlo Park, CA). pTK-RL plasmid was purchased from Promega (Madison, WI).

    Article Title: Epidermal Growth Factor Promotes Protein Degradation of Epithelial Protein Lost in Neoplasm (EPLIN), a Putative Metastasis Suppressor, during Epithelial-mesenchymal Transition
    Article Snippet: Human EPLIN-β reporter was obtained from SwitchGear Genomics (Menlo Park, CA). pTK-RL plasmid was purchased from Promega (Madison, WI).

    Article Title: Hyperactivation of Stat3 in gp130 mutant mice promotes gastric hyperproliferation and desensitizes TGF-beta signaling.
    Article Snippet: The latent transcription factor Stat3 is activated by gp130, the common receptor for the interleukin (IL)-6 cytokine family and other growth factor and cytokine receptors.. Ligand-induced dimerization of gp130 leads to activation of the Stat1, Stat3 and Shp2-Ras-Erk signaling pathways.. Here we assess genetically the contribution of exaggerated Stat3 activation to the phenotype of gp130Y757F/Y757F mice, in which a knock-in mutation disrupts the negative feedback mechanism on gp130-dependent Stat signaling.

    Article Title: Inhibition of serotonin-induced mitogenesis, migration, and ERK MAPK nuclear translocation in vascular smooth muscle cells by atorvastatin.
    Article Snippet: Li M, Liu Y, Dutt P, Fanburg BL, Toksoz D. Inhibition of serotonin-induced mitogenesis, migration, and ERK MAPK nuclear translocation in vascular smooth muscle cells by atorvastatin.. Am J Physiol Lung Cell Mol Physiol 293: L463–L471, 2007.. First published June 1, 2007; doi:10.1152/ajplung.00133.2007.—The HMGCoA reductase inhibitors, statins, have pleiotropic effects which may include interference with the isoprenylation of Ras and Rho small GTPases.

    Article Title: Helicoverpa armigera GATAe transcriptional factor regulates the expression of Bacillus thuringiensis Cry1Ac receptor gene ABCC2 by its interplay with additional transcription factors.
    Article Snippet: Helicoverpa armigera is a worldwide pest that has been efficiently controlled by transgenic plants expressing Bt Cry toxins.. To exert toxicity, Cry toxins bind to different receptors located in larval midgut cells.. Previously, we reported that GATA transcription factor GATAe activates the expression of multiple H. armigera Cry1Ac receptors in different insect cell lines.

    Transfection:

    Article Title: Mfn2 downregulation in excitotoxicity causes mitochondrial dysfunction and delayed neuronal death
    Article Snippet: Firefly luciferase-based reporter gene was transfected along with a Renilla expression vector (pTK-RL; Promega), and also, where relevant, MEF2-DN expression vector.

    Article Title: Epidermal Growth Factor Promotes Protein Degradation of Epithelial Protein Lost in Neoplasm (EPLIN), a Putative Metastasis Suppressor, during Epithelial-mesenchymal Transition
    Article Snippet: Plasmids and Constructs—GFP-tagged expression plasmid for EPLIN- (pCMV-EPLIN-AC-GFP) and control plasmid pCMV-AC-GFP were purchased from Origene (Rockville, MD).HumanEPLIN- reporterwas obtained fromSwitchGear Genomics (Menlo Park, CA). pTK-RL plasmid was purchased from Promega (Madison, WI).

    Article Title: Epidermal Growth Factor Promotes Protein Degradation of Epithelial Protein Lost in Neoplasm (EPLIN), a Putative Metastasis Suppressor, during Epithelial-mesenchymal Transition
    Article Snippet: Human EPLIN-β reporter was obtained from SwitchGear Genomics (Menlo Park, CA). pTK-RL plasmid was purchased from Promega (Madison, WI).

    Article Title: Hyperactivation of Stat3 in gp130 mutant mice promotes gastric hyperproliferation and desensitizes TGF-beta signaling.
    Article Snippet: The latent transcription factor Stat3 is activated by gp130, the common receptor for the interleukin (IL)-6 cytokine family and other growth factor and cytokine receptors.. Ligand-induced dimerization of gp130 leads to activation of the Stat1, Stat3 and Shp2-Ras-Erk signaling pathways.. Here we assess genetically the contribution of exaggerated Stat3 activation to the phenotype of gp130Y757F/Y757F mice, in which a knock-in mutation disrupts the negative feedback mechanism on gp130-dependent Stat signaling.

    Article Title: Inhibition of serotonin-induced mitogenesis, migration, and ERK MAPK nuclear translocation in vascular smooth muscle cells by atorvastatin.
    Article Snippet: Li M, Liu Y, Dutt P, Fanburg BL, Toksoz D. Inhibition of serotonin-induced mitogenesis, migration, and ERK MAPK nuclear translocation in vascular smooth muscle cells by atorvastatin.. Am J Physiol Lung Cell Mol Physiol 293: L463–L471, 2007.. First published June 1, 2007; doi:10.1152/ajplung.00133.2007.—The HMGCoA reductase inhibitors, statins, have pleiotropic effects which may include interference with the isoprenylation of Ras and Rho small GTPases.

    Article Title: Helicoverpa armigera GATAe transcriptional factor regulates the expression of Bacillus thuringiensis Cry1Ac receptor gene ABCC2 by its interplay with additional transcription factors.
    Article Snippet: Helicoverpa armigera is a worldwide pest that has been efficiently controlled by transgenic plants expressing Bt Cry toxins.. To exert toxicity, Cry toxins bind to different receptors located in larval midgut cells.. Previously, we reported that GATA transcription factor GATAe activates the expression of multiple H. armigera Cry1Ac receptors in different insect cell lines.

    Control:

    Article Title: Mfn2 downregulation in excitotoxicity causes mitochondrial dysfunction and delayed neuronal death
    Article Snippet: Firefly luciferase-based reporter gene was transfected along with a Renilla expression vector (pTK-RL; Promega), and also, where relevant, MEF2-DN expression vector.

    Article Title: Epidermal Growth Factor Promotes Protein Degradation of Epithelial Protein Lost in Neoplasm (EPLIN), a Putative Metastasis Suppressor, during Epithelial-mesenchymal Transition
    Article Snippet: Plasmids and Constructs—GFP-tagged expression plasmid for EPLIN- (pCMV-EPLIN-AC-GFP) and control plasmid pCMV-AC-GFP were purchased from Origene (Rockville, MD).HumanEPLIN- reporterwas obtained fromSwitchGear Genomics (Menlo Park, CA). pTK-RL plasmid was purchased from Promega (Madison, WI).

    Article Title: Epidermal Growth Factor Promotes Protein Degradation of Epithelial Protein Lost in Neoplasm (EPLIN), a Putative Metastasis Suppressor, during Epithelial-mesenchymal Transition
    Article Snippet: Human EPLIN-β reporter was obtained from SwitchGear Genomics (Menlo Park, CA). pTK-RL plasmid was purchased from Promega (Madison, WI).

    Article Title: Hyperactivation of Stat3 in gp130 mutant mice promotes gastric hyperproliferation and desensitizes TGF-beta signaling.
    Article Snippet: The latent transcription factor Stat3 is activated by gp130, the common receptor for the interleukin (IL)-6 cytokine family and other growth factor and cytokine receptors.. Ligand-induced dimerization of gp130 leads to activation of the Stat1, Stat3 and Shp2-Ras-Erk signaling pathways.. Here we assess genetically the contribution of exaggerated Stat3 activation to the phenotype of gp130Y757F/Y757F mice, in which a knock-in mutation disrupts the negative feedback mechanism on gp130-dependent Stat signaling.

    Article Title: Inhibition of serotonin-induced mitogenesis, migration, and ERK MAPK nuclear translocation in vascular smooth muscle cells by atorvastatin.
    Article Snippet: Li M, Liu Y, Dutt P, Fanburg BL, Toksoz D. Inhibition of serotonin-induced mitogenesis, migration, and ERK MAPK nuclear translocation in vascular smooth muscle cells by atorvastatin.. Am J Physiol Lung Cell Mol Physiol 293: L463–L471, 2007.. First published June 1, 2007; doi:10.1152/ajplung.00133.2007.—The HMGCoA reductase inhibitors, statins, have pleiotropic effects which may include interference with the isoprenylation of Ras and Rho small GTPases.

    Article Title: Helicoverpa armigera GATAe transcriptional factor regulates the expression of Bacillus thuringiensis Cry1Ac receptor gene ABCC2 by its interplay with additional transcription factors.
    Article Snippet: Helicoverpa armigera is a worldwide pest that has been efficiently controlled by transgenic plants expressing Bt Cry toxins.. To exert toxicity, Cry toxins bind to different receptors located in larval midgut cells.. Previously, we reported that GATA transcription factor GATAe activates the expression of multiple H. armigera Cry1Ac receptors in different insect cell lines.



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    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
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    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
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    Promega transfection control renilla luciferase plasmid ptk-rl
    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
    Transfection Control Renilla Luciferase Plasmid Ptk Rl, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    transfection control renilla luciferase plasmid ptk-rl - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

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    Millipore ptk rl plasmid 21–170
    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
    Ptk Rl Plasmid 21–170, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptk-rl+plasmid/ptk+rl+plasmid+21+170/pm35569307-70-3-7
    Average 90 stars, based on 1 article reviews
    ptk rl plasmid 21–170 - by Bioz Stars, 2026-10
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    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative luciferase (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by Renilla luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.

    Journal: Cancer Science

    Article Title: Application of prime editing system to introduce TP53 R248Q hotspot mutation in acute lymphoblastic leukemia cell line

    doi: 10.1111/cas.16162

    Figure Lengend Snippet: Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative luciferase (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by Renilla luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.

    Article Snippet: Renilla luciferase reporter plasmid pTK‐RL (E2231; Promega) was cotransfected as the internal standard.

    Techniques: Activity Assay, Targeted Gene Expression, Reporter Assay, Luciferase, Cell Culture, Produced, Expressing, Quantitative RT-PCR