john gurdon (Addgene inc)

Addgene inc is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Addgene inc john gurdon
John Gurdon, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psuper/pSUPER+retro+puro+Scr+shRNA+(Plasmid+%2330520)/us12496328-255-13-15
Average 93 stars, based on 18 article reviews

john gurdon - by Bioz Stars, 2026-09

93/100 stars

Images

other:

Article Title: Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Catalase Sigma-Aldrich C40 Acryloyl-X-SE Thermo Fisher Scientific A20770 Acrylamide 40% Sigma-Aldrich A4058 N,N0-Methylenebisacrylamide Sigma-Aldrich M1533 Sodium acrylate Sigma-Aldrich 408220 TEMED Bio-Rad 1610800 APS Sigma-Aldrich A3678 anhydrous-DMSO Thermo Fisher Scientific D12345 Guanidine HCl Sigma-Aldrich G3272 Triton-X-100 Sigma-Aldrich 93433 Proteinase K Thermo Fisher Scientific EO0492 0.1% (w/v) poly-L-lysine Sigma-Aldrich P8920 Deposited data Proteomics data P180 pulldown This study PXD050948 RNA-sequencing data P180 This study GSE262262 Experimental models: Cell lines HEK-293T ATCC CRL-3216 Experimental models: Organisms/strains Rat (Wistar) Janvier N/A Recombinant DNA pSuper Brummelkamp et al.74 N/A pGW1-mCherry Kapitein et al.75 N/A pGW1-BFP Kapitein et al.75 N/A RTN4A-GFP Kind gift from dr. Voeltz (Howard Hughes Medical Institute & Department of Molecular, Cellular and Developmental Biology, University of Colorado) Addgene #61807 pEGFP(A206K)-N1 Kind gift from dr. Lippincott-Schwartz- (Howard Hughes Medical Institute, Janelia Research Campus) N/A pEGFP(A206K)-C1 Kind gift from dr. Lippincott-Schwartz (Howard Hughes Medical Institute, Janelia Research Campus) N/A GFP-Sec61b Kind gift from dr. Rapaport (Department of Cell Biology, Harvard Medical School) Addgene #15108 DP1-GFP Farı́as et al.12 N/A RpL10A-tagRFP Kind gift from dr. Singer (Department of Cell Biology, Albert Einstein College of Medicine) Addgene #74172 TOM20-V5-FKBP-AP Kind gift from dr. Ting (Department of Genetics& Biology, Stanford University) Addgene #120914 mCherrymyr5’/3’-Calreticulin Kind gift from dr. Twiss (Department Biological Sciences, University of South Carolina) N/A Lifeact-mCherry Kind gift from dr. Sato (Graduate School of Arts and Sciences, University of Tokyo) Addgene #67302 HA-KifC1-MD-Strep Farı́as et al.12 N/A GFP-SBP-RTN4A Farı́as et al.12 N/A P180-DCoiled-coil-GFP Farı́as et al.12 N/A P180-DCoiled-coil-GFPAviTag This study N/A P180-Drepeats-GFP Farı́as et al.12 N/A P180-Drepeats-GFPAviTag This study N/A P180-Coiled-coil-GFPAviTag This study N/A P180-Repeats-GFPAviTag This study N/A SplitAP-V5-C1 Özkan et al.19 N/A (Continued on next page) ll OPEN ACCESS Article e2 Developmental Cell 59, 2053–2068.e1–e9, August 19, 2024

Mutagenesis:

Article Title: RN7SK small nuclear RNA controls bidirectional transcription of highly expressed gene pairs in skin
Article Snippet: .. To over-express the wild-type or siRNA 5 resistant mutant RN7SK construct, the full-length RNA was cloned into pSUPER (Addgene). ..

Article Title: Nucleocytoplasmic transport of the RNA-binding protein CELF2 regulates neural stem cell fates.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Sox2 (goat) R&D Systems AF2018 bIII-tubulin (mouse) Biolegend 801201 CELF2 (rabbit) Abcam ab186430 CELF2 (mouse) Santa Cruz Biotechnology sc47731 Ki67 (mouse) BD Biosciences 550609 CSDE1 (rabbit) Abcam ab201688 LaminB1 (mouse) Abcam ab8982 Lap2 (mouse) BD Biosciences 611000 Satb2 (mouse) Abcam ab51502 Tbr2 (rabbit) Abcam ab183991 Tbr2 (rat) Invitrogen 14-4875-80 Pax6 (rabbit) Biolegend 901301 pH3 (mouse) Invitrogen MA5-15220 pH3 (mouse) Cell Signaling 9706s GFP (mouse) Abcam ab290 GFP (chicken) EMD Millipore AB16901 GAPDH (rabbit) Sigma G9545 Dcp1a (mouse) Abnova H00055802-M06 4E-T Abnova H00056478-B01 Bacterial strains pEGFP-C1 Clontech Discontinued pCAGIG Matsuda and Cepko, 2004 Addgene (11159) pSUPER OligoEngine VEC-pBS-0002 Chemicals, peptides, and recombinant proteins Q5 Site-Directed Mutagenesis Kit NEB E0554S Polyethylenimine Sigma Aldrich 765090 FGF2 Corning CB-40060A B27 Thermo Fisher 17504044 Penicillin-streptomycin Wisent 450-201-EL L-glutamine Wisent 609-065-EL DMEM Thermo Fisher 11995065 Neurobasal Thermo Fisher 21103049 FBS Wisent 098-150 Lipofectamine Stem Thermo Fisher STEM00015 Laminin Corning 354232 Poly-D-Lysine Sigma Aldrich P6407 Trizol Invitrogen LS15596026 Maxima H Minus cDNA Synthesis Kit Thermo Fisher M1681 Phusion High Fidelity PCR Master Mix Thermo Fisher F531 PerfeCTa SYBR Green FastMix Quanta Bio 95072 Bovine Serum Albumin (BSA) BioShop 005PJD Bovine Serum Albumin (BSA) Jackson Immunoresearch 005PEF Hoechst 33258 Sigma Aldrich 94403 (Continued on next page) Cell Reports 35, 109226, June 8, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Aqua-Poly/Mount Polysciences 18606 SUPERasine-IN Invitrogen AM2694 Critical commercial assays EZ-Magna RIP-RNA Immunoprecipitation Kit EMD Millipore 17-700 RNAscope Fluorescent Multiplex Acdbio 320851 Tbr2 mRNA probe Acdbio 429641 Duolink In Situ Orange Starter Kit Mouse/Rabbit Sigma Aldrich DUO92102 NEBNext Ultra II Directional RNA library prep kit NEB E6350S NEBNext rRNA depletion kit NEB E6350S Deposited data Raw and analyzed data This paper GSE163703 Experimental models: Cell lines HEK293 ATCC CRL-1573 Experimental models: Organisms/strains CD1 mouse Charles River 022 Oligonucleotides See Table S4 for oligonucleotide information.

Construct:

Article Title: A simple and robust vector-based shRNA expression system used for RNA interference.
Article Snippet: .. Plasmid construction The HBV replication-competent plasmids pHBV1.31 and pHBV1.18 containing 1.31 and 1.18 HBV genome copies, respectively, were constructed and preserved in our laboratory (unpublished data), pmWasabi-N was a gift from Allele Biotechnology Company, pSuper was obtained from Addgene (Cambridge, MA), vector pSEAP2-Control was a gift from Dr. Shuiping Liu (Central South University of China), and the pCMVGLuc vector was purchased from New England Biolabs (NEB, Ipswich, MA). ..

Clone Assay:

Article Title: Production of functional small interfering RNAs by an amino-terminal deletion mutant of human Dicer
Article Snippet: .. First, a 257-bp fragment of the RLuc CDS was PCR-amplified and cloned into pSuper (Addgene) between two Pol III-dependent promoters, H1 and U6. ..

Recombinant:

cDNA Synthesis:

Polymerase Chain Reaction:

SYBR Green Assay:

Plasmid Preparation:

Article Title: SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling.
Article Snippet: VPS35 was subcloned into pDESTeGFP-C1 (Addgene plasmid #31796; Hong et al., 2010) usingGateway LRClonase II (ThermoFisher). .. Rab5-dsRed andRab11-dsRed were obtained fromAddgene (plasmid #13050 and #12679, respectively; Sharma et al., 2003; Choudhury et al., 2002). pSuper-dsRed was a gift from Kumiko Ui-Tei (Addgene plasmid #42053; Nishi et al., 2013). ..

Article Title: CUE domain‐containing protein 2 promotes the Warburg effect and tumorigenesis
Article Snippet: .. Briefly, plasmid in pSin‐EF2α‐Puro (Addgene), pLKO, or pSUPER viral vector was cotransfected with virus packaging plasmids into HEK293T cells using Lipofectamine 2000 (Invitrogen). .. Viral supernatant was collected and added into culture medium of PLC, HeLa, or MDA‐MB‐231 cells in the presence of 8 μg/ml polybrene (Sigma‐Aldrich).

Virus:

Transfection:

Article Title: Population of ATP synthase molecules in mitochondria is limited by available 6.8-kDa proteolipid protein (MLQ).
Article Snippet: Makoto Fujikawa, Shigenori Ohsakaya, Kanako Sugawara and Masasuke Yoshida* JST ICORP ATP-Synthesis Regulation Project, 2-3-6 Aomi, Koto-ku, Tokyo 135-0064, Japan Department of Biochemistry, Faculty of Pharmaceutical Science, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, Japan Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259-R1-8, Midori-ku, Yokohama 226-8503, Japan Department of Molecular Bioscience, Kyoto Sangyo University, Kamigamo-Motoyama, Sakyo-ku, Kyoto 603-8555, Japan

Review