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Cell Signaling Technology Inc antibodies against pstat6
Antibodies Against Pstat6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pstat6/Phospho-Stat6+(Tyr641)+Antibody/pm41796250-226-11-14
Average 95 stars, based on 388 article reviews

antibodies against pstat6 - by Bioz Stars, 2026-10

95/100 stars

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Article Title: Loss of tumor suppressors promotes inflammatory tumor microenvironment and enhances LAG3+T cell mediated immune suppression
Article Snippet: An equal amount of total proteins was denatured, separated on NuPAGE 4–12% Bis-Tris protein gels, and transferred to a nitrocellulose membrane (Bio-Rad, #1704270). .. Membranes were incubated with primary antibodies against Cas9 (Diagenode, C15200203, 7A9), NF1 (abcam, ab17963), TSC1 (ThermoFisher, PA5-20131), TGF-βR2 (R&D System, AF532), NF2 (Cell Signaling, #6995, D1D8), Pten (Cell Signaling, #9559, 138G6), Caspase 3 (Cell Signaling, #9665, 8G10), pAKT (Cell Signaling, #9271), AKT (Cell Signaling, #9272), pERK (Cell Signaling, #9101), ERK (Santa Cruz, sc-514302, C-9), pmTOR (Santa Cruz, sc-293133, 59.Ser2448), HER2 (Cell Signaling, #2165, 29D8), CD40 (R&D System, AF440), pSTAT3 (Cell Signaling, #9145S, D3A7), STAT3 (Cell Signaling, #4904S, 79D7), pSTAT6 (Cell Signaling, #56554S, D8S9Y and 9361S), STAT6 (Cell Signaling, #9362S), pJAK3 (Cell Signaling, #5031S, D44E3), JAK3 (Cell Signaling, #8863S, D7B12), or β-actin (Santa Cruz, sc-69879, AC-15). ..

Article Title: Memory T‐Cell Phenotype in Cutaneous T‐Cell Lymphoma Is Modified by Germline Gene Gametocyte Specific Factor 1
Article Snippet: To evaluate protein expression, we performed western blotting with 4%–20% Mini‐PROTEAN TGX Stain‐Free Precast Gels (Bio‐rad Cat# 4568093 and 4568094), followed by transfer to 0.22 μm PVDF membranes (Bio‐rad Cat# 1704157 and 1704156). .. Overnight incubation at 4°C was carried out with the following primary antibodies: DDX4 (Abcam Cat# ab27591 RRID:AB_11139638), GAPDH (Thermo Fisher Scientific Cat# PA1‐987 RRID:AB_2107311), GTSF1 (Abcam Cat# ab262937), LINE‐1 Orf1p (Millipore Sigma Cat# MABC1152 RRID:AB_2941775), NFκB2 p100/p52 (Cell Signaling Cat# 4882 RRID:AB_10695537), PIWIL2 (Abcam Cat# ab181340), PIWIL4 (Abcam Cat# ab111714 RRID:AB_10887762), pSTAT3 Y705 (Cell Signaling Cat# 9145 RRID:AB_2491009), STAT3 (Cell Signaling Cat# 30835 RRID:AB_2798995), STAT4 (Cell Signaling Cat# 2653, RRID:AB_2255156), STAT5 (Cell Signaling Cat# 94205 RRID:AB_2737403), pSTAT6 (Cell Signaling Cat# 9361 RRID:AB_331595), STAT6 (Cell Signaling Cat# 5397 RRID:AB_11220421) and TDRD9 (Abcam Cat# ab118427). .. Secondary antibody incubation for 1 h was performed with the appropriate HRP‐conjugated secondary antibody: anti‐mouse IgG (Cell Signaling Cat# 7076 RRID:AB_330924) or anti‐rabbit IgG (Cell Signaling Cat# 7074 RRID:AB_2099233).

Article Title: Loss of tumor suppressors promotes inflammatory tumor microenvironment and enhances LAG3+T cell mediated immune suppression.
Article Snippet: An equal amount of total proteins was denatured, separated on NuPAGE 4–12% Bis-Tris protein gels, and transferred to a nitrocellulose membrane (Bio-Rad, #1704270). .. Membranes were incubated with primary antibodies against Cas9 (Diagenode, C15200203, 7A9), NF1 (abcam, ab17963), TSC1 (ThermoFisher, PA5-20131), TGF-βR2 (R&D System, AF532), NF2 (Cell Signaling, #6995, D1D8), Pten (Cell Signaling, #9559, 138G6), Caspase 3 (Cell Signaling, #9665, 8G10), pAKT (Cell Signaling, #9271), AKT (Cell Signaling, #9272), pERK (Cell Signaling, #9101), ERK (Santa Cruz, sc-514302, C-9), pmTOR (Santa Cruz, sc-293133, 59.Ser2448), HER2 (Cell Signaling, #2165, 29D8), CD40 (R&D System, AF440), pSTAT3 (Cell Signaling, #9145S, D3A7), STAT3 (Cell Signaling, #4904S, 79D7), pSTAT6 (Cell Signaling, #56554S, D8S9Y and 9361S), STAT6 (Cell Signaling, #9362S), pJAK3 (Cell Signaling, #5031S, D44E3), JAK3 (Cell Signaling, #8863S, D7B12), or β-actin (Santa Cruz, sc-69879, AC-15). ..

Ubiquitin Proteomics:

Article Title: DNA-PK-mediated phosphorylation of STAT6 establishes a non-canonical type 2 immunity axis to prevent macrophage senescence.
Article Snippet: Then rProtein A/G Beads (Cell Signaling, # 37478) were added and incubated for 4 h. The immunoprecipitated was washed three times with buffer and followed by immunoblot. .. Antibodies used were as follows: HSP90 (Santa Cruz, #sc-13119, 1:10,000), α-Tubulin (Sigma, #T6199, 1:10,000), STAT6 (Cell Signaling, #5397, 1:1500), pSTAT6(pY641) (Cell Signaling, #56554, 1:1000), pSTAT6(pS807) (ABclonal Tech, customized, 1:200), DNA-PK (Santa Cruz, #sc-390849, 1:500), ATM (Cell Signaling, #2873, 1:1000), ATR (Santa Cruz, #sc-515173, 1:500), CHK1 (Cell Signaling, #2360, 1:1500), CHK2 (Proteintech, #13954-1-AP, 1:1000), p16-INK4A (Abcam, #ab211542, 1:1000), γH2AX (Cell Signaling, #9718, 1:1500), BRCA1 (Thermo Fisher, #MA1-23164, 1:1000), UBE2T (Thermo Fisher, #PA576202, 1:1000), JAK (Cell Signaling, #50996, 1:1000), STING (Cell Signaling, #13647, AR TI CL E IN P RE SS 1:2000), STING (pS365) (Cell Signaling, #72971, 1:1000), pTBK1 (pS172) (Cell Signaling, #5483, 1:1000), pJAK1 (pY1034/1035) (Cell Signaling, #74129, 1:1000), Ubiquitin (Cell Signaling, #3936, 1:1000), K48-specific ubiquitin (Abcam, #ab140601, 1:1000), PU.1 (Abcam, #ab227835, 1:1500), Col1a1 (Cell Signaling, #72026, 1:1000), SMAD2/3 (Cell Signaling, #8685, 1:1000), pSMAD2 (Ser465/467)/SMAD3 (Ser423/425) (Cell Signaling, #8828, 1:1000), αSMA (Cell Signaling, #19245, 1:2000) anti-HA (Cell Signaling, #3724, 1:5000) and anti-Flag (Cell Signaling, #8146, 1:5000). .. Generation of STAT6 pS807-sepicific antibody To generate a polyclonal antibody specific for STAT6 phosphorylated at Ser807 (pS807), we collaborated with ABclonal Tech.

Article Title: DNA-PK-mediated phosphorylation of STAT6 establishes a non-canonical type 2 immunity axis to prevent macrophage senescence
Article Snippet: Then, rProtein A/G Beads (Cell Signaling, # 37478) were added and incubated for 4 h. The immunoprecipitated was washed three times with buffer and followed by immunoblot. .. Antibodies used were as follows: HSP90 (Santa Cruz, #sc-13119, 1:10,000), α-Tubulin (Sigma, #T6199, 1:10,000), STAT6 (Cell Signaling, #5397, 1:1500), pSTAT6(pY641) (Cell Signaling, #56554, 1:1000), pSTAT6(pS807) (ABclonal Tech, customized, 1:200), DNA-PK (Santa Cruz, #sc-390849, 1:500), ATM (Cell Signaling, #2873, 1:1000), ATR (Santa Cruz, #sc-515173, 1:500), CHK1 (Cell Signaling, #2360, 1:1500), CHK2 (Proteintech, #13954-1-AP, 1:1000), p16-INK4A (Abcam, #ab211542, 1:1000), γH2AX (Cell Signaling, #9718, 1:1500), BRCA1 (Thermo Fisher, #MA1-23164, 1:1000), UBE2T (Thermo Fisher, # PA576202 , 1:1000), JAK (Cell Signaling, #50996, 1:1000), STING (Cell Signaling, #13647, 1:2000), STING (pS365) (Cell Signaling, #72971, 1:1000), pTBK1 (pS172) (Cell Signaling, #5483, 1:1000), pJAK1 (pY1034/1035) (Cell Signaling, #74129, 1:1000), Ubiquitin (Cell Signaling, #3936, 1:1000), K48-specific ubiquitin (Abcam, #ab140601, 1:1000), PU.1 (Abcam, #ab227835, 1:1500), Col1a1 (Cell Signaling, #72026, 1:1000), SMAD2/3 (Cell Signaling, #8685, 1:1000), p-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) (Cell Signaling, #8828, 1:1000), αSMA (Cell Signaling, #19245, 1:2000) anti-HA (Cell Signaling, #3724, 1:5000) and anti-Flag (Cell Signaling, #8146, 1:5000). .. To generate a polyclonal antibody specific for STAT6 phosphorylated at Ser807 (pS807), we collaborated with ABclonal Tech.

other:

Article Title: A Hirsutella sinensis Alcohol Extract Exerts Bidirectional Immunoregulatory Effects by Regulating Macrophage Polarization.
Article Snippet: For background, Hirsutella sinensis, the only anamorphic fungus considered an effective substitute for Cordyceps sinensis, possesses immunoregulatory properties.. However, the specific mechanism underlying the immunoregulatory function of Hirsutella sinensis remains unclear.. The purpose is to investigate the therapeutic effects of Hirsutella sinensis alcohol extract (HSAE) on immune dysregulation and elucidate the underlying mechanisms involved.

Image Search Results

A Immunoblots of pSTAT6 (pY641) and γH2AX in thioglycolate-elicited peritoneal macrophages (Thio-PM) with Etoposide (Eto) and IL-4 treatment. The right panel shows the relative intensity of pSTAT6 (pY641) ( n = 4 independent experiments). B Immunofluorescence images of staining (pSTAT6 (pY641), red; DAPI, blue) of IL-4-stimulated Thio-PMs treated with Eto. Scale bars 50 μm. Right panel shows the quantification of pSTAT6 (pY641) ( n = 3 biological replicates per group). C Immunoblots of pSTAT6 (pY641) and γH2AX in Eto-treated Thio-PMs with different inhibitors. KU55933, an inhibitor of ATM. Berzosertib, inhibitor of ATR. NU7026, inhibitor of DNA-PK. AZD7762, inhibitor of CHK1/2. The right panel shows the quantification of pSTAT6 (pY641) ( n = 3 independent experiments). D Immunoblots of pSTAT6 (pY641) and γH2AX in Eto-treated Cas9 tg/+ Thio-PMs with indicated sgRNA. The right panel shows the quantification of pSTAT6 (pY641)/tSTAT6 ( n = 3 independent experiments). E Dual luciferase reporter of STAT6 in RAW 264.7 cells treated with Eto and NU7026 ( n = 3 per group). Data are mean ± s.e.m. p-value was calculated by paired two-tailed Student’s t test ( A , D ), unpaired two-tailed Student’s t test ( B ), one-way ANOVA with Dunnett’s correction ( C , E ).

Journal: Nature Communications

Article Title: DNA-PK-mediated phosphorylation of STAT6 establishes a non-canonical type 2 immunity axis to prevent macrophage senescence

doi: 10.1038/s41467-026-69996-8

Figure Lengend Snippet: A Immunoblots of pSTAT6 (pY641) and γH2AX in thioglycolate-elicited peritoneal macrophages (Thio-PM) with Etoposide (Eto) and IL-4 treatment. The right panel shows the relative intensity of pSTAT6 (pY641) ( n = 4 independent experiments). B Immunofluorescence images of staining (pSTAT6 (pY641), red; DAPI, blue) of IL-4-stimulated Thio-PMs treated with Eto. Scale bars 50 μm. Right panel shows the quantification of pSTAT6 (pY641) ( n = 3 biological replicates per group). C Immunoblots of pSTAT6 (pY641) and γH2AX in Eto-treated Thio-PMs with different inhibitors. KU55933, an inhibitor of ATM. Berzosertib, inhibitor of ATR. NU7026, inhibitor of DNA-PK. AZD7762, inhibitor of CHK1/2. The right panel shows the quantification of pSTAT6 (pY641) ( n = 3 independent experiments). D Immunoblots of pSTAT6 (pY641) and γH2AX in Eto-treated Cas9 tg/+ Thio-PMs with indicated sgRNA. The right panel shows the quantification of pSTAT6 (pY641)/tSTAT6 ( n = 3 independent experiments). E Dual luciferase reporter of STAT6 in RAW 264.7 cells treated with Eto and NU7026 ( n = 3 per group). Data are mean ± s.e.m. p-value was calculated by paired two-tailed Student’s t test ( A , D ), unpaired two-tailed Student’s t test ( B ), one-way ANOVA with Dunnett’s correction ( C , E ).

Article Snippet: Antibodies used were as follows: HSP90 (Santa Cruz, #sc-13119, 1:10,000), α-Tubulin (Sigma, #T6199, 1:10,000), STAT6 (Cell Signaling, #5397, 1:1500), pSTAT6(pY641) (Cell Signaling, #56554, 1:1000), pSTAT6(pS807) (ABclonal Tech, customized, 1:200), DNA-PK (Santa Cruz, #sc-390849, 1:500), ATM (Cell Signaling, #2873, 1:1000), ATR (Santa Cruz, #sc-515173, 1:500), CHK1 (Cell Signaling, #2360, 1:1500), CHK2 (Proteintech, #13954-1-AP, 1:1000), p16-INK4A (Abcam, #ab211542, 1:1000), γH2AX (Cell Signaling, #9718, 1:1500), BRCA1 (Thermo Fisher, #MA1-23164, 1:1000), UBE2T (Thermo Fisher, # PA576202 , 1:1000), JAK (Cell Signaling, #50996, 1:1000), STING (Cell Signaling, #13647, 1:2000), STING (pS365) (Cell Signaling, #72971, 1:1000), pTBK1 (pS172) (Cell Signaling, #5483, 1:1000), pJAK1 (pY1034/1035) (Cell Signaling, #74129, 1:1000), Ubiquitin (Cell Signaling, #3936, 1:1000), K48-specific ubiquitin (Abcam, #ab140601, 1:1000), PU.1 (Abcam, #ab227835, 1:1500), Col1a1 (Cell Signaling, #72026, 1:1000), SMAD2/3 (Cell Signaling, #8685, 1:1000), p-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) (Cell Signaling, #8828, 1:1000), αSMA (Cell Signaling, #19245, 1:2000) anti-HA (Cell Signaling, #3724, 1:5000) and anti-Flag (Cell Signaling, #8146, 1:5000).

Techniques: Western Blot, Immunofluorescence, Staining, Luciferase, Two Tailed Test

A Co-IP of STAT6-Turbo and JAK1, DNA-PK, ATM, and ATR in RAW 264.7 cells with TurboID. B Proximity ligation assay (PLA) of STAT6 and DNA-PK in Thio-PMs treated with Eto. Scale bars 10 μm. C The quantification of PLA signal in ( B ) ( n = 3 biological replicates per group, with more than 100 cells). D Diagram of phosphorylated sites in mouse STAT6 after Eto treatment. E Immunoblots of pSTAT6 (pY641) and tSTAT6 in Stat6 -/- Thio-PMs expressing STAT6 mutants (WT, S807A, S807E). F Immunoblots of pSTAT6 (pY641) in STAT6-mutants-expressed Cas9 tg+ Thio-PMs with Prkdc sgRNA under IL-4 & Eto co-treatment. G Dual luciferase reporter of STAT6 in RAW 264.7 cells expressing STAT6 mutants under IL-4 treatment ( n = 3 biological replicates per group). H Immunoblots of pSTAT6 (pS807) and γH2AX in Eto-treated Cas9 tg+ Thio-PMs with Prkdc sgRNAs. I Immunoblots of pSTAT6 (pS807) and γH2AX in Eto-treated Cas9 tg/+ Thio-PMs with Ku70 or Ku80 sgRNA. J Immunoblots of pSTAT6 (pS807) and γH2AX in Eto-treated Thio-PMs pre-treated with NU7026. The right panel shows the relative intensity of pSTAT6 (pS807) ( n = 3 independent experiments). K Immunoblots of pSTAT6 (pS807) and pSTAT6 (pY641) in Thio-PMs with indicated treatment. AS1517499, an inhibitor of STAT6 (block of pY641). L Immunoblots of pSTAT6 (pS807) and tSTAT6 in Eto-treated Stat6 -/- Thio-PMs expressing STAT6 mutants (WT, Y641F). The blot results are representative of three biologically independent repeats in ( A , E , F , H , I , K and L ). Data are mean ± s.e.m. Unpaired two-tailed Welch’s t test ( C ), One-way ANOVA with Dunnett’s correction ( G ), paired two-tailed Student’s t test ( J ).

Journal: Nature Communications

Article Title: DNA-PK-mediated phosphorylation of STAT6 establishes a non-canonical type 2 immunity axis to prevent macrophage senescence

doi: 10.1038/s41467-026-69996-8

Figure Lengend Snippet: A Co-IP of STAT6-Turbo and JAK1, DNA-PK, ATM, and ATR in RAW 264.7 cells with TurboID. B Proximity ligation assay (PLA) of STAT6 and DNA-PK in Thio-PMs treated with Eto. Scale bars 10 μm. C The quantification of PLA signal in ( B ) ( n = 3 biological replicates per group, with more than 100 cells). D Diagram of phosphorylated sites in mouse STAT6 after Eto treatment. E Immunoblots of pSTAT6 (pY641) and tSTAT6 in Stat6 -/- Thio-PMs expressing STAT6 mutants (WT, S807A, S807E). F Immunoblots of pSTAT6 (pY641) in STAT6-mutants-expressed Cas9 tg+ Thio-PMs with Prkdc sgRNA under IL-4 & Eto co-treatment. G Dual luciferase reporter of STAT6 in RAW 264.7 cells expressing STAT6 mutants under IL-4 treatment ( n = 3 biological replicates per group). H Immunoblots of pSTAT6 (pS807) and γH2AX in Eto-treated Cas9 tg+ Thio-PMs with Prkdc sgRNAs. I Immunoblots of pSTAT6 (pS807) and γH2AX in Eto-treated Cas9 tg/+ Thio-PMs with Ku70 or Ku80 sgRNA. J Immunoblots of pSTAT6 (pS807) and γH2AX in Eto-treated Thio-PMs pre-treated with NU7026. The right panel shows the relative intensity of pSTAT6 (pS807) ( n = 3 independent experiments). K Immunoblots of pSTAT6 (pS807) and pSTAT6 (pY641) in Thio-PMs with indicated treatment. AS1517499, an inhibitor of STAT6 (block of pY641). L Immunoblots of pSTAT6 (pS807) and tSTAT6 in Eto-treated Stat6 -/- Thio-PMs expressing STAT6 mutants (WT, Y641F). The blot results are representative of three biologically independent repeats in ( A , E , F , H , I , K and L ). Data are mean ± s.e.m. Unpaired two-tailed Welch’s t test ( C ), One-way ANOVA with Dunnett’s correction ( G ), paired two-tailed Student’s t test ( J ).

Techniques: Co-Immunoprecipitation Assay, Proximity Ligation Assay, Western Blot, Expressing, Luciferase, Blocking Assay, Two Tailed Test

A Hypothetical model of regulation of pSTAT6 (pY641). B Immunoblots of pSTAT6 (pY641) and tSTAT6 in STAT6-mutants (WT, S807E)-expressed RAW 264.7 cells under IL-4 stimulation for 30 min or 4 h. C Quantification of pSTAT6 (pY641)/tSTAT6 in ( B ) ( n = 3 independent experiments). D Stabilization assay of STAT6 mutants (WT, S807E) in IL-4-stimulated RAW 264.7 cells under CHX treatment. E Degradation curve of pSTAT6 (pY641) in ( D ) ( n = 3 independent experiments). F Immunoblots of pSTAT6 (pY641) and tSTAT6 in MG132-pre-treated Stat6 -/- PMs expressing STAT6 mutants (WT, S807E) under IL-4 stimulation for 4 h. G Immunoblots of ubiquitination and K48-ubiquitination on STAT6 in Thio-PMs with indicated treatments. The bottom right panel shows the quantification of STAT6 ubiquitination ( n = 3 independent experiments). H Co-IP of RNF128-FLAG with STAT6-HA mutants (WT, S807E) in RAW 264.7 cells after 4-h IL-4 stimulation. I The quantification of the interaction between STAT6 and RNF128 in ( H ) ( n = 4 independent experiments). J Immunoblots of ubiquitination on STAT6-HA mutants (WT, S807E) and their interaction with RNF128-FLAG in RAW 264.7 cells after 4 h IL-4 stimulation. K Immunoblots of ubiquitination on STAT6-HA mutants (WT, K319R, K595R, K319R/K595R) in RAW 264.7 cells after 4-h IL-4 stimulation. L Stability assay of STAT6 mutants (WT, K319R, K595R) in RAW 264.7 cells under CHX treatment. M Stability assay of STAT6 mutants (WT, S807A, S807A/K319R) in RAW 264.7 cells under CHX treatment. N Diagram of pS807 effect on activated STAT6 stability. The blot data are representative of three independent repeats ( F and J – M ). Data are mean ± s.e.m. Paired two-tailed t test ( C , I ), ordinary two-way ANOVA ( E ), one-way ANOVA with Dunnett’s correction ( G ).

Journal: Nature Communications

Article Title: DNA-PK-mediated phosphorylation of STAT6 establishes a non-canonical type 2 immunity axis to prevent macrophage senescence

doi: 10.1038/s41467-026-69996-8

Figure Lengend Snippet: A Hypothetical model of regulation of pSTAT6 (pY641). B Immunoblots of pSTAT6 (pY641) and tSTAT6 in STAT6-mutants (WT, S807E)-expressed RAW 264.7 cells under IL-4 stimulation for 30 min or 4 h. C Quantification of pSTAT6 (pY641)/tSTAT6 in ( B ) ( n = 3 independent experiments). D Stabilization assay of STAT6 mutants (WT, S807E) in IL-4-stimulated RAW 264.7 cells under CHX treatment. E Degradation curve of pSTAT6 (pY641) in ( D ) ( n = 3 independent experiments). F Immunoblots of pSTAT6 (pY641) and tSTAT6 in MG132-pre-treated Stat6 -/- PMs expressing STAT6 mutants (WT, S807E) under IL-4 stimulation for 4 h. G Immunoblots of ubiquitination and K48-ubiquitination on STAT6 in Thio-PMs with indicated treatments. The bottom right panel shows the quantification of STAT6 ubiquitination ( n = 3 independent experiments). H Co-IP of RNF128-FLAG with STAT6-HA mutants (WT, S807E) in RAW 264.7 cells after 4-h IL-4 stimulation. I The quantification of the interaction between STAT6 and RNF128 in ( H ) ( n = 4 independent experiments). J Immunoblots of ubiquitination on STAT6-HA mutants (WT, S807E) and their interaction with RNF128-FLAG in RAW 264.7 cells after 4 h IL-4 stimulation. K Immunoblots of ubiquitination on STAT6-HA mutants (WT, K319R, K595R, K319R/K595R) in RAW 264.7 cells after 4-h IL-4 stimulation. L Stability assay of STAT6 mutants (WT, K319R, K595R) in RAW 264.7 cells under CHX treatment. M Stability assay of STAT6 mutants (WT, S807A, S807A/K319R) in RAW 264.7 cells under CHX treatment. N Diagram of pS807 effect on activated STAT6 stability. The blot data are representative of three independent repeats ( F and J – M ). Data are mean ± s.e.m. Paired two-tailed t test ( C , I ), ordinary two-way ANOVA ( E ), one-way ANOVA with Dunnett’s correction ( G ).

Techniques: Western Blot, Expressing, Ubiquitin Proteomics, Co-Immunoprecipitation Assay, Stability Assay, Two Tailed Test

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