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pstat2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pstat2
    Pstat2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pstat2/Phospho-Stat2+(Tyr690)+Rabbit+mAb/pmc12817915-187-37-58
    Average 96 stars, based on 188 article reviews
    pstat2 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: ISGF3 and STAT2/IRF9 Control Basal and IFN-Induced Transcription through Genome-Wide Binding of Phosphorylated and Unphosphorylated Complexes to Common ISRE-Containing ISGs
    Article Snippet: .. Protein isolation, quantification and immunoblotting were performed as described before by Piaszyk-Borychowska [ ] with the use of the primary antibodies anti-pSTAT1 (CST, 7649, D4A7, 1:200), -pSTAT2 (CST, 88410, D3P2P, 1:500), -tSTAT1 (CST, 14994, D1K9Y, 1:500), -STAT2 (CST, 72604, D9J7L, 1:500), -IRF9 (CST, 76684, D2T8M, 1:400 in case of 2fTGH and U3C-based cells and 1:300 in case of Huh cells) as well as -α-tubulin (Abcam, Waltham, MA, USA, AB52866, 1:2000) and the secondary HRP-conjugated goat anti-rabbit antibodies (Sigma-Aldrich, A9169, 1:20,000). .. The total RNA was extracted using the GeneMATRIX Universal RNA Purification Kit (EurX, Gdansk, Poland, E3598-02) according to the protocol provided by the manufacturer.

    Article Title: ISGF3 and STAT2/IRF9 Control Basal and IFN-Induced Transcription through Genome-Wide Binding of Phosphorylated and Unphosphorylated Complexes to Common ISRE-Containing ISGs.
    Article Snippet: .. Protein isolation, quantification and immunoblotting were performed as described before by Piaszyk-Borychowska [20] with the use of the primary antibodies anti-pSTAT1 (CST, 7649, D4A7, 1:200), -pSTAT2 (CST, 88410, D3P2P, 1:500), -tSTAT1 (CST, 14994, D1K9Y, 1:500), -STAT2 (CST, 72604, D9J7L, 1:500), -IRF9 (CST, 76684, D2T8M, 1:400 in case of 2fTGH and U3C-based cells and 1:300 in case of Huh cells) as well as -α-tubulin (Abcam, Waltham, MA, USA, AB52866, 1:2000) and the secondary HRP-conjugated goat anti-rabbit antibodies (Sigma-Aldrich, A9169, 1:20,000). .. The total RNA was extracted using the GeneMATRIX Universal RNA Purification Kit (EurX, Gdansk, Poland, E3598-02) according to the protocol provided by the manufacturer.

    Western Blot:

    Article Title: ISGF3 and STAT2/IRF9 Control Basal and IFN-Induced Transcription through Genome-Wide Binding of Phosphorylated and Unphosphorylated Complexes to Common ISRE-Containing ISGs
    Article Snippet: .. Protein isolation, quantification and immunoblotting were performed as described before by Piaszyk-Borychowska [ ] with the use of the primary antibodies anti-pSTAT1 (CST, 7649, D4A7, 1:200), -pSTAT2 (CST, 88410, D3P2P, 1:500), -tSTAT1 (CST, 14994, D1K9Y, 1:500), -STAT2 (CST, 72604, D9J7L, 1:500), -IRF9 (CST, 76684, D2T8M, 1:400 in case of 2fTGH and U3C-based cells and 1:300 in case of Huh cells) as well as -α-tubulin (Abcam, Waltham, MA, USA, AB52866, 1:2000) and the secondary HRP-conjugated goat anti-rabbit antibodies (Sigma-Aldrich, A9169, 1:20,000). .. The total RNA was extracted using the GeneMATRIX Universal RNA Purification Kit (EurX, Gdansk, Poland, E3598-02) according to the protocol provided by the manufacturer.

    Article Title: ISGF3 and STAT2/IRF9 Control Basal and IFN-Induced Transcription through Genome-Wide Binding of Phosphorylated and Unphosphorylated Complexes to Common ISRE-Containing ISGs.
    Article Snippet: .. Protein isolation, quantification and immunoblotting were performed as described before by Piaszyk-Borychowska [20] with the use of the primary antibodies anti-pSTAT1 (CST, 7649, D4A7, 1:200), -pSTAT2 (CST, 88410, D3P2P, 1:500), -tSTAT1 (CST, 14994, D1K9Y, 1:500), -STAT2 (CST, 72604, D9J7L, 1:500), -IRF9 (CST, 76684, D2T8M, 1:400 in case of 2fTGH and U3C-based cells and 1:300 in case of Huh cells) as well as -α-tubulin (Abcam, Waltham, MA, USA, AB52866, 1:2000) and the secondary HRP-conjugated goat anti-rabbit antibodies (Sigma-Aldrich, A9169, 1:20,000). .. The total RNA was extracted using the GeneMATRIX Universal RNA Purification Kit (EurX, Gdansk, Poland, E3598-02) according to the protocol provided by the manufacturer.

    Membrane:

    Article Title: Supporting Information for Type I interferon signaling pathway enhances immune-checkpoint inhibition in KRAS mutant lung tumors
    Article Snippet: 20 μg of protein extract was mixed with the NuPAGETM 4X sample buffer (Invitrogen, NP0007) and the NuPAGETM 10X sample reducing agent (Invitrogen, NP0009) and heated for 5 minutes at 95oC. .. Protein extracts were separated on NUPAGETM 4-12% BisTris Midi gels (Invitrogen, 10452342), transferred to a nitrocellulose membrane (Amersham, 10600001) and blotted with antibodies raised against: pSTAT2 (1:1000, Cell Signaling, 4441), STAT2 (1 μg/ml, R&D Systems, MAB16662), pSTAT1 (1:1000, Cell Signaling, 9167), STAT1 (1:1000, Cell Signaling, 9175) pERK1/2 (1:1000, Cell Signaling, 9101), ERK1 (1:1000, BD Pharmingen, 554100), ERK2 (1:500, BD Biosciences, 610103). .. Primary antibodies were detected with HRP-conjugated secondary antibodies directed against mouse, rat or rabbit IgGs (1:2000, HRP, Dako, P0447, P0450 and P0448, respectively) or fluorescent-dye conjugated secondary antibody against mouse (1:5000, Alexa Fluor 680, Invitrogen A21057) and visualized with ChemiLuminiscence ECL Western blot detection reagent (GE Healthcare, 28980926) or Odyssey® CLx Imager (LI-COR Biosciences).

    Cell-Signaling:

    Article Title: Short-chain fatty acids butyrate and acetate limit Zika virus replication and associated ocular manifestations via the G-protein coupled receptor 43/FFAR2.
    Article Snippet: .. Antibodies used in this study were purchased from the following sources: 4G2 (GeneTex, #GTX57154), NS3 (GeneTex, #GTX133309), β-actin (Millipore Sigma, #A2228), pNFκB (#3033), NFκB (#6956) pERK1/2 (#4370), ERK (#4695), pP38 (#4511), P38 (#8690), pSTAT1 (#9167), STAT1 (#14994), pSTAT2 (#88410), STAT2 (#72604), pSTAT3 (#9145), STAT3 (#9139), RIG-I (#3743), pIRF3 (#79945), IRF3 (#4302), and IFIT2 (#92633) antibodies were purchased from Cell Signaling Technology (Danvers, MA). .. SCFAs NaAc (Thermofisher, #A13184-30), 4-PBA (#11323), NaB (#13121), and FFAR2 receptor antagonist 4-CMTB (#29680) were purchased from Cayman Chemicals (Ann Arbor, MI).

    Article Title: Short-chain fatty acids butyrate and acetate limit Zika virus replication and associated ocular manifestations via the G-protein coupled receptor 43/FFAR2
    Article Snippet: .. Antibodies used in this study were purchased from the following sources: 4G2 (GeneTex, #GTX57154), NS3 (GeneTex, #GTX133309), β-actin (Millipore Sigma, #A2228), pNFκB (#3033), NFκB (#6956) pERK1/2 (#4370), ERK (#4695), pP38 (#4511), P38 (#8690), pSTAT1 (#9167), STAT1 (#14994), pSTAT2 (#88410), STAT2 (#72604), pSTAT3 (#9145), STAT3 (#9139), RIG-I (#3743), pIRF3 (#79945), IRF3 (#4302), and IFIT2 (#92633) antibodies were purchased from Cell Signaling Technology (Danvers, MA). .. SCFAs NaAc (Thermofisher, #A13184-30), 4-PBA (#11323), NaB (#13121), and FFAR2 receptor antagonist 4-CMTB (#29680) were purchased from Cayman Chemicals (Ann Arbor, MI).

    Staining:

    Article Title: Specificity and promiscuity of JAK recruitment regulates pleiotropy of cytokine-receptor signaling
    Article Snippet: .. Compensation was performed using single stained samples to account for spillover of Pacific Blue and AlexaFluor-488 into the APC channel. (Antibody: pSTAT1: BD Phosflow 612597, pSTAT2: cell signaling 907405, pSTAT3: BD Phosflow 557815, pSTAT5: BD Phosflow 612599, pSTAT6: BD Phosflow 612601). pSTAT activation by TPOR in the presence of different JAKs was probed by phospho-flow cytometry as described previously( ). ..

    Activation Assay:

    Article Title: Specificity and promiscuity of JAK recruitment regulates pleiotropy of cytokine-receptor signaling
    Article Snippet: .. Compensation was performed using single stained samples to account for spillover of Pacific Blue and AlexaFluor-488 into the APC channel. (Antibody: pSTAT1: BD Phosflow 612597, pSTAT2: cell signaling 907405, pSTAT3: BD Phosflow 557815, pSTAT5: BD Phosflow 612599, pSTAT6: BD Phosflow 612601). pSTAT activation by TPOR in the presence of different JAKs was probed by phospho-flow cytometry as described previously( ). ..

    Cytometry:

    Article Title: Specificity and promiscuity of JAK recruitment regulates pleiotropy of cytokine-receptor signaling
    Article Snippet: .. Compensation was performed using single stained samples to account for spillover of Pacific Blue and AlexaFluor-488 into the APC channel. (Antibody: pSTAT1: BD Phosflow 612597, pSTAT2: cell signaling 907405, pSTAT3: BD Phosflow 557815, pSTAT5: BD Phosflow 612599, pSTAT6: BD Phosflow 612601). pSTAT activation by TPOR in the presence of different JAKs was probed by phospho-flow cytometry as described previously( ). ..



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    IFIT3 knockdown relieved the intestinal inflammatory response and inhibited the activation of the STAT1/2 pathway in DSS-induced UC mice. ( A and B ) Comparison of colon transduction efficacy in mice between intraperitoneal injection of saline and AAV9 using paraffin sections ( A ) and the IVIS Lumina III imaging system ( B ). Scale bar = 100 µm. ( C ) Comparison of colon length between AAV9-shNC- and AAV9-shIFIT3-treated UC mice. ( D ) Weight changes in the two mouse groups. ( E ) Representative images and histological score of H&E staining in colon tissues from the two mouse groups. Scale bar = 100 µm (top) and 25 µm (bottom). ( F ) mRNA levels of IFIT3, TNF-α, IL-1β and IL-6 in colon tissues from the two mouse groups. ( G ) Protein expressions of STAT1, pSTAT1, STAT2, <t>pSTAT2</t> and IFIT3 in colon tissues from the two mouse groups. Data was presented as mean ± SD (n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001.
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    IFIT3 knockdown relieved the intestinal inflammatory response and inhibited the activation of the STAT1/2 pathway in DSS-induced UC mice. ( A and B ) Comparison of colon transduction efficacy in mice between intraperitoneal injection of saline and AAV9 using paraffin sections ( A ) and the IVIS Lumina III imaging system ( B ). Scale bar = 100 µm. ( C ) Comparison of colon length between AAV9-shNC- and AAV9-shIFIT3-treated UC mice. ( D ) Weight changes in the two mouse groups. ( E ) Representative images and histological score of H&E staining in colon tissues from the two mouse groups. Scale bar = 100 µm (top) and 25 µm (bottom). ( F ) mRNA levels of IFIT3, TNF-α, IL-1β and IL-6 in colon tissues from the two mouse groups. ( G ) Protein expressions of STAT1, pSTAT1, STAT2, <t>pSTAT2</t> and IFIT3 in colon tissues from the two mouse groups. Data was presented as mean ± SD (n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001.
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    Image Search Results


    IFIT3 knockdown relieved the intestinal inflammatory response and inhibited the activation of the STAT1/2 pathway in DSS-induced UC mice. ( A and B ) Comparison of colon transduction efficacy in mice between intraperitoneal injection of saline and AAV9 using paraffin sections ( A ) and the IVIS Lumina III imaging system ( B ). Scale bar = 100 µm. ( C ) Comparison of colon length between AAV9-shNC- and AAV9-shIFIT3-treated UC mice. ( D ) Weight changes in the two mouse groups. ( E ) Representative images and histological score of H&E staining in colon tissues from the two mouse groups. Scale bar = 100 µm (top) and 25 µm (bottom). ( F ) mRNA levels of IFIT3, TNF-α, IL-1β and IL-6 in colon tissues from the two mouse groups. ( G ) Protein expressions of STAT1, pSTAT1, STAT2, pSTAT2 and IFIT3 in colon tissues from the two mouse groups. Data was presented as mean ± SD (n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Journal of Inflammation Research

    Article Title: Downregulation of IFIT3 Relieves the Inflammatory Response in Ulcerative Colitis via Selectively Regulating Macrophage M1 Polarization and the STAT1/2 Signaling Pathway

    doi: 10.2147/JIR.S542033

    Figure Lengend Snippet: IFIT3 knockdown relieved the intestinal inflammatory response and inhibited the activation of the STAT1/2 pathway in DSS-induced UC mice. ( A and B ) Comparison of colon transduction efficacy in mice between intraperitoneal injection of saline and AAV9 using paraffin sections ( A ) and the IVIS Lumina III imaging system ( B ). Scale bar = 100 µm. ( C ) Comparison of colon length between AAV9-shNC- and AAV9-shIFIT3-treated UC mice. ( D ) Weight changes in the two mouse groups. ( E ) Representative images and histological score of H&E staining in colon tissues from the two mouse groups. Scale bar = 100 µm (top) and 25 µm (bottom). ( F ) mRNA levels of IFIT3, TNF-α, IL-1β and IL-6 in colon tissues from the two mouse groups. ( G ) Protein expressions of STAT1, pSTAT1, STAT2, pSTAT2 and IFIT3 in colon tissues from the two mouse groups. Data was presented as mean ± SD (n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: The PVDF membranes were incubated with primary antibodies against IFIT3 (1:3000; #15201-1-AP, Proteintect), STAT1 (1:1000; # R25799 , Zenbio, Chengdu, China), pSTAT1 (1:1000; #bsm-52209R, Bioss, Beijing, China), STAT2 (1:1000; #R381419 Zenbio), pSTAT2 (1:1000; #bs-3428R, Bioss), and GAPDH (1:1000; #GB15002-100, Servicebio) overnight at 4 °C and then incubated with HRP-labeled secondary antibodies at room temperature for 1 hour.

    Techniques: Knockdown, Activation Assay, Comparison, Transduction, Injection, Saline, Imaging, Staining

    IFIT3 knockdown repressed macrophage M1 polarization by inhibiting the STAT1 pathway in vitro. ( A and B ) mRNA and protein levels of IFIT3 in untreated THP‐1 macrophages or treated with 100 ng/mL LPS or 100 ng/mL LPS + 20 ng/mL IFN‐γ or 20 ng/mL IL‐4. ( C ) mRNA levels of IFIT3 in 100 ng/mL LPS-stimulated macrophages treated with siNC or two siRNAs targeting IFIT3 (siIFIT3-1 and siIFIT3-2). ( D ) mRNA levels of TNF-α, IL-1β and IL-6 in 100 ng/mL LPS-stimulated macrophages treated with siNC and siIFIT3-2. ( E ) Protein levels of STAT1, pSTAT1, STAT2, pSTAT2 and IFIT3 in each group. ( F ) Representative gating and the co-expression of CD86 and CD68 in untreated (M0) and 100 ng/mL LPS-stimulated macrophages (M1) by flow cytometry. ( G ) Representative gating and the co-expression of CD68 and CD86 in 100 ng/mL LPS-stimulated macrophages treated with siNC and siIFIT3-2 by flow cytometry. Data was presented as mean ± SD from 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Journal of Inflammation Research

    Article Title: Downregulation of IFIT3 Relieves the Inflammatory Response in Ulcerative Colitis via Selectively Regulating Macrophage M1 Polarization and the STAT1/2 Signaling Pathway

    doi: 10.2147/JIR.S542033

    Figure Lengend Snippet: IFIT3 knockdown repressed macrophage M1 polarization by inhibiting the STAT1 pathway in vitro. ( A and B ) mRNA and protein levels of IFIT3 in untreated THP‐1 macrophages or treated with 100 ng/mL LPS or 100 ng/mL LPS + 20 ng/mL IFN‐γ or 20 ng/mL IL‐4. ( C ) mRNA levels of IFIT3 in 100 ng/mL LPS-stimulated macrophages treated with siNC or two siRNAs targeting IFIT3 (siIFIT3-1 and siIFIT3-2). ( D ) mRNA levels of TNF-α, IL-1β and IL-6 in 100 ng/mL LPS-stimulated macrophages treated with siNC and siIFIT3-2. ( E ) Protein levels of STAT1, pSTAT1, STAT2, pSTAT2 and IFIT3 in each group. ( F ) Representative gating and the co-expression of CD86 and CD68 in untreated (M0) and 100 ng/mL LPS-stimulated macrophages (M1) by flow cytometry. ( G ) Representative gating and the co-expression of CD68 and CD86 in 100 ng/mL LPS-stimulated macrophages treated with siNC and siIFIT3-2 by flow cytometry. Data was presented as mean ± SD from 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: The PVDF membranes were incubated with primary antibodies against IFIT3 (1:3000; #15201-1-AP, Proteintect), STAT1 (1:1000; # R25799 , Zenbio, Chengdu, China), pSTAT1 (1:1000; #bsm-52209R, Bioss, Beijing, China), STAT2 (1:1000; #R381419 Zenbio), pSTAT2 (1:1000; #bs-3428R, Bioss), and GAPDH (1:1000; #GB15002-100, Servicebio) overnight at 4 °C and then incubated with HRP-labeled secondary antibodies at room temperature for 1 hour.

    Techniques: Knockdown, In Vitro, Expressing, Flow Cytometry

    UPA inhibits inflammation by silencing IFIT3 in LPS-stimulated macrophages. ( A ) mRNA levels of IFIT3, TNF-α, IL-1β and IL-6 in 100 ng/mL LPS-stimulated macrophages with untreated or treated with 100 nM and 400 nM UPA. ( B ) Protein levels of STAT1, pSTAT1, STAT2, pSTAT2 and IFIT3 among cell-groups. ( C ) Schematic diagram of relation among UPA, IFIT3 and STAT1/2 pathways. Data was presented as mean ± SD from 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Journal of Inflammation Research

    Article Title: Downregulation of IFIT3 Relieves the Inflammatory Response in Ulcerative Colitis via Selectively Regulating Macrophage M1 Polarization and the STAT1/2 Signaling Pathway

    doi: 10.2147/JIR.S542033

    Figure Lengend Snippet: UPA inhibits inflammation by silencing IFIT3 in LPS-stimulated macrophages. ( A ) mRNA levels of IFIT3, TNF-α, IL-1β and IL-6 in 100 ng/mL LPS-stimulated macrophages with untreated or treated with 100 nM and 400 nM UPA. ( B ) Protein levels of STAT1, pSTAT1, STAT2, pSTAT2 and IFIT3 among cell-groups. ( C ) Schematic diagram of relation among UPA, IFIT3 and STAT1/2 pathways. Data was presented as mean ± SD from 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: The PVDF membranes were incubated with primary antibodies against IFIT3 (1:3000; #15201-1-AP, Proteintect), STAT1 (1:1000; # R25799 , Zenbio, Chengdu, China), pSTAT1 (1:1000; #bsm-52209R, Bioss, Beijing, China), STAT2 (1:1000; #R381419 Zenbio), pSTAT2 (1:1000; #bs-3428R, Bioss), and GAPDH (1:1000; #GB15002-100, Servicebio) overnight at 4 °C and then incubated with HRP-labeled secondary antibodies at room temperature for 1 hour.

    Techniques: