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m propidium iodide  (Biotium)


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    Structured Review

    Biotium m propidium iodide
    M Propidium Iodide, supplied by Biotium, used in various techniques. Bioz Stars score: 96/100, based on 320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/propidium+iodide/Propidium+Iodide/pmc13650049-386-31-35
    Average 96 stars, based on 320 article reviews
    m propidium iodide - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Single Cell:

    Article Title: KMT2C inactivation leads to PTEN downregulation and tolerance to DNA damage during cell cycle progression.
    Article Snippet: .. Single cell suspensions were generated with trypsinization and fixed with ethanol overnight before stained with propidium iodide (50mg/ml, Biotium, cat. 40017) for 30min. .. Flow cytometry was performed on a FC500 Beckman Coulter and for the analysis FlowJo v.10 was used.

    Generated:

    Article Title: KMT2C inactivation leads to PTEN downregulation and tolerance to DNA damage during cell cycle progression.
    Article Snippet: .. Single cell suspensions were generated with trypsinization and fixed with ethanol overnight before stained with propidium iodide (50mg/ml, Biotium, cat. 40017) for 30min. .. Flow cytometry was performed on a FC500 Beckman Coulter and for the analysis FlowJo v.10 was used.

    Staining:

    Article Title: KMT2C inactivation leads to PTEN downregulation and tolerance to DNA damage during cell cycle progression.
    Article Snippet: .. Single cell suspensions were generated with trypsinization and fixed with ethanol overnight before stained with propidium iodide (50mg/ml, Biotium, cat. 40017) for 30min. .. Flow cytometry was performed on a FC500 Beckman Coulter and for the analysis FlowJo v.10 was used.

    Article Title: Multi-omics and spatial analysis of microgravity-grown glioblastoma organoids reveals superior modeling of advanced disease after long-term spaceflight
    Article Snippet: After 48 hours, the organoids were stained with Calcein-AM and imaged (Nikon AXR), with maximum intensity Z projections used for representative images. .. For viability staining, organoids were transferred to a 96-well plate and stained with Calcein-AM (1:500, BioLegend, 425201) and propidium iodide (2 μg/ml, Biotium, 40016) for 30 minutes at 37°C. .. Z-stack images of each entire organoid were taken using a confocal microscope (Nikon AXR), and a maximum intensity projection was generated for visualization of representative organoids.

    Article Title: FANCD2 restrains fork progression and prevents fragility at early origins upon re-replication
    Article Snippet: Cells were then washed with PBS 1% BSA and incubated with 0.5% Triton X-100 in PBS for 10 min. EdU staining was performed using the BaseClick EdU-HTS 488 kit (Sigma-Aldrich, BCK-HTS488-2) according to the manufacturer’s instructions. .. The genomic DNA was stained with propidium iodide (Biotium, 40017) in combination with RNAse (AppliChem, A2760). .. For Annexin V/PI flow cytometry Annexin V Apoptosis Detection Kit (BD PharmingenTM, 556547) was used, according to manufacturer’s instructions.

    other:

    Article Title: Succinate dehydrogenase loss suppresses pyrimidine biosynthesis via succinate-mediated inhibition of aspartate transcarbamylase.
    Article Snippet: Quantitation of all metabolites was performed using Tracefinder v.4.1 (Thermo Scientific) referencing an in-house metabolite standards library using ≤5 ppm mass error.

    Article Title: Cellular reprogramming to reverse aging and promote organ and tissue regeneration
    Article Snippet: SH-SY5Y neuroblastoma cells were obtained from the American Tissue Culture Collection (ATCC, CRL-2266) and maintained according to ATCC recommendations.

    Article Title: Hydrophobic polymeric particles of the plant polyester suberin destroy bacteria by interacting with their membranes
    Article Snippet: Deuterated dimethyl sulfoxide (DMSO- d 6 , >99.99 %) was obtained from Merck; Diethyl ether (>99.7 %) from PanReac; Hydrochloric acid (37 %) from Riedel-de-Haën; and Ethyl 4-hydroxy-3- methoxy-cinnamate (99 %) and ciprofloxacin hydrochloride monohydrate from Alfa Aesar.

    Electroporation:

    Article Title: Innovative nano-electrode chip for electroporation in yeast
    Article Snippet: .. Propidium iodide (PI, Biotium, USA), an impermeant nucleic acid dye, was used as an electroporation marker. ..

    Marker:

    Article Title: Innovative nano-electrode chip for electroporation in yeast
    Article Snippet: .. Propidium iodide (PI, Biotium, USA), an impermeant nucleic acid dye, was used as an electroporation marker. ..



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    Representative images of cell cycle analysis by <t>propidium</t> iodide staining using flow cytometry of A549 cells treated with control GapmeR compared with GRAS1 GapmeR1 or GRAS1 GapmeR2 (left). Quantification of the proportion of cells in each cell cycle phase was performed from three independent experiments (right). Error bars represent SD in all panels; (ns) P > 0.05; (*) P ≤ 0.05; (***) P < 0.001 by two-tailed t test.
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    Representative images of cell cycle analysis by <t>propidium</t> iodide staining using flow cytometry of A549 cells treated with control GapmeR compared with GRAS1 GapmeR1 or GRAS1 GapmeR2 (left). Quantification of the proportion of cells in each cell cycle phase was performed from three independent experiments (right). Error bars represent SD in all panels; (ns) P > 0.05; (*) P ≤ 0.05; (***) P < 0.001 by two-tailed t test.
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    Image Search Results


    Commensal microflora coatings support adhesion and viability of human cells. (A) CLSM images showing the morphology and distribution of gingival fibroblasts, oral keratinocytes, and macrophages after 48 h on glass, Ti, and CHM. Scale bar: 100 μm. (B) Metabolic activity of (a) fibroblasts, (b) keratinocytes, and (c) macrophages after 48 h on the indicated surfaces. Cells were stained with green fluorescent Calcein AM to label viable cells and with red fluorescent propidium iodide (PI) to label nonviable (dead) cells. All experiments were conducted using three biological replicates (N = 3) and three technical replicates per group (n = 3). For CLSM, the biocompatibility assay was imaged at 10× magnification with a voxel size of 1.52 μm (x) × 1.52 μm (y) × 3 μm (z).

    Journal: Bioactive Materials

    Article Title: Commensal microbiota–coated biohybrid implants induce antibiofilm, osteogenic, and immunomodulatory responses in a human 3D immunocompetent model

    doi: 10.1016/j.bioactmat.2026.06.006

    Figure Lengend Snippet: Commensal microflora coatings support adhesion and viability of human cells. (A) CLSM images showing the morphology and distribution of gingival fibroblasts, oral keratinocytes, and macrophages after 48 h on glass, Ti, and CHM. Scale bar: 100 μm. (B) Metabolic activity of (a) fibroblasts, (b) keratinocytes, and (c) macrophages after 48 h on the indicated surfaces. Cells were stained with green fluorescent Calcein AM to label viable cells and with red fluorescent propidium iodide (PI) to label nonviable (dead) cells. All experiments were conducted using three biological replicates (N = 3) and three technical replicates per group (n = 3). For CLSM, the biocompatibility assay was imaged at 10× magnification with a voxel size of 1.52 μm (x) × 1.52 μm (y) × 3 μm (z).

    Article Snippet: Ti- and CHM-integrated tissues, after coculture under sterile or biofilm-infected conditions, were longitudinally sectioned at the tissue–implant interface and stained with Calcein AM and propidium iodide (Invitrogen, Germany) for 30 min.

    Techniques: Activity Assay, Staining

    Representative images of cell cycle analysis by propidium iodide staining using flow cytometry of A549 cells treated with control GapmeR compared with GRAS1 GapmeR1 or GRAS1 GapmeR2 (left). Quantification of the proportion of cells in each cell cycle phase was performed from three independent experiments (right). Error bars represent SD in all panels; (ns) P > 0.05; (*) P ≤ 0.05; (***) P < 0.001 by two-tailed t test.

    Journal: Life Science Alliance

    Article Title: The noncoding RNA GRAS1 interacts with NKAP and protects against DNA damage and cell death

    doi: 10.26508/lsa.202403105

    Figure Lengend Snippet: Representative images of cell cycle analysis by propidium iodide staining using flow cytometry of A549 cells treated with control GapmeR compared with GRAS1 GapmeR1 or GRAS1 GapmeR2 (left). Quantification of the proportion of cells in each cell cycle phase was performed from three independent experiments (right). Error bars represent SD in all panels; (ns) P > 0.05; (*) P ≤ 0.05; (***) P < 0.001 by two-tailed t test.

    Article Snippet: Then cells were resuspended in 100 μl of 1X Annexin-binding buffer (10 mM HEPES adjusted to pH 7.4, 140 mM NaCl, and 2.5 mM CaCl2) with 5 μl of FITC-conjugated Annexin V (A13199; Life Technologies) and 2 μl of 1 mg/ml propidium iodide (40017; Biotium).

    Techniques: Cell Cycle Assay, Staining, Flow Cytometry, Control, Two Tailed Test