mammalian expression vectors prk5-megfp-hmgb1-wt (Addgene inc)
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Mammalian Expression Vectors Prk5 Megfp Hmgb1 Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prk5+vector/prk5+ha+gst+raga+q66l/pm40468084-568-0-13
Average 90 stars, based on 1 article reviews
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Clone Assay:Article Title: Differential HspBP1 expression accounts for the greater vulnerability of neurons than astrocytes to misfolded proteins Article Snippet: Htt-23Q, -73Q, and-130Q were generated by subcloning N-terminal fragments of huntingtin (1–230 amino acids) containing 23Q, 73Q, or 130Q into pDendra2-N (Clontech) using SalI and ApaI cloning sites. .. Murine HspBP1 was cloned into a Article Title: AMBRA1 Negatively Regulates the Function of ALDH1B1, a Cancer Stem Cell Marker, by Controlling Its Ubiquitination Article Snippet: .. Wild-type and mutant ubiquitins cloned into the Article Title: AMPK attenuates SHH subgroup medulloblastoma growth and metastasis by inhibiting NF-κB activation. Article Snippet: D283-Med cells (ATCC Cat# HTB-185, RRID:CVCL_1155) were cultured in MEM medium supplemented with 10% FBS. .. Full-length human PRKAA1 cDNA was generated by PCR and cloned into the Article Title: Autophagy regulates spermatid differentiation via degradation of PDLIM1. Article Snippet: The rat anti-LAMP2 antibody (ab13524, Abcam) was kindly provided by Dr. Li Yu (Tsinghua University). .. Plasmids , cell culture and transfection Genes encoding FLAG-tagged PDLIM1, STMN1 (stathmin 1), PPP4R2 (protein phosphatase 4, regulatory subunit 2), NUDC (nudC nuclear distribution protein), MAP6 (microtubule-associated protein 6), YBX1 (Y box protein 1), and CD2AP (CD2-associated protein) were cloned into the SalI and NotI site of the modified Plasmid Preparation:Article Title: Differential HspBP1 expression accounts for the greater vulnerability of neurons than astrocytes to misfolded proteins Article Snippet: Htt-23Q, -73Q, and-130Q were generated by subcloning N-terminal fragments of huntingtin (1–230 amino acids) containing 23Q, 73Q, or 130Q into pDendra2-N (Clontech) using SalI and ApaI cloning sites. .. Murine HspBP1 was cloned into a Article Title: AMBRA1 Negatively Regulates the Function of ALDH1B1, a Cancer Stem Cell Marker, by Controlling Its Ubiquitination Article Snippet: .. Wild-type and mutant ubiquitins cloned into the Article Title: miR-27a-3p negatively regulates osteogenic differentiation of MC3T3-E1 preosteoblasts by targeting osterix Article Snippet: Primary antibodies against Osx (1:4,000; cat. no. ab94744; Abcam) and β-actin (1:1,000; cat. no. sc-47778; Santa Cruz Biotechnology, Inc.) were used. .. Osx cDNA was inserted into a Article Title: AMPK attenuates SHH subgroup medulloblastoma growth and metastasis by inhibiting NF-κB activation. Article Snippet: D283-Med cells (ATCC Cat# HTB-185, RRID:CVCL_1155) were cultured in MEM medium supplemented with 10% FBS. .. Full-length human PRKAA1 cDNA was generated by PCR and cloned into the Article Title: Calpain Determines the Propensity of Adult Hippocampal Neural Stem Cells to Autophagic Cell Death Following Insulin Withdrawal. Article Snippet: .. pRK5-calpain 1 and -calpain 2 plasmids were constructed by subcloning the human calpain 1 (OriGene, Rockville, MD, http://www.origene.com; SC116897) and calpain 2 (OriGene; SC119079) genes into the Article Title: Autophagy regulates spermatid differentiation via degradation of PDLIM1. Article Snippet: The rat anti-LAMP2 antibody (ab13524, Abcam) was kindly provided by Dr. Li Yu (Tsinghua University). .. Plasmids , cell culture and transfection Genes encoding FLAG-tagged PDLIM1, STMN1 (stathmin 1), PPP4R2 (protein phosphatase 4, regulatory subunit 2), NUDC (nudC nuclear distribution protein), MAP6 (microtubule-associated protein 6), YBX1 (Y box protein 1), and CD2AP (CD2-associated protein) were cloned into the SalI and NotI site of the modified Cloning:Article Title: Differential HspBP1 expression accounts for the greater vulnerability of neurons than astrocytes to misfolded proteins Article Snippet: Htt-23Q, -73Q, and-130Q were generated by subcloning N-terminal fragments of huntingtin (1–230 amino acids) containing 23Q, 73Q, or 130Q into pDendra2-N (Clontech) using SalI and ApaI cloning sites. .. Murine HspBP1 was cloned into a Mutagenesis:Article Title: AMBRA1 Negatively Regulates the Function of ALDH1B1, a Cancer Stem Cell Marker, by Controlling Its Ubiquitination Article Snippet: .. Wild-type and mutant ubiquitins cloned into the Construct:Article Title: Frizzled-4 C-terminus Distal to KTXXXW Motif is Essential for Normal Dishevelled Recruitment and Norrin-stimulated Activation of Lef/Tcf-dependent Transcriptional Activation Article Snippet: .. The mouse FZD 4 construct containing the V5-tag between F37 and G38 in a Article Title: Calpain Determines the Propensity of Adult Hippocampal Neural Stem Cells to Autophagic Cell Death Following Insulin Withdrawal. Article Snippet: .. pRK5-calpain 1 and -calpain 2 plasmids were constructed by subcloning the human calpain 1 (OriGene, Rockville, MD, http://www.origene.com; SC116897) and calpain 2 (OriGene; SC119079) genes into the Generated:Article Title: AMPK attenuates SHH subgroup medulloblastoma growth and metastasis by inhibiting NF-κB activation. Article Snippet: D283-Med cells (ATCC Cat# HTB-185, RRID:CVCL_1155) were cultured in MEM medium supplemented with 10% FBS. .. Full-length human PRKAA1 cDNA was generated by PCR and cloned into the Polymerase Chain Reaction:Article Title: AMPK attenuates SHH subgroup medulloblastoma growth and metastasis by inhibiting NF-κB activation. Article Snippet: D283-Med cells (ATCC Cat# HTB-185, RRID:CVCL_1155) were cultured in MEM medium supplemented with 10% FBS. .. Full-length human PRKAA1 cDNA was generated by PCR and cloned into the Subcloning:Article Title: Calpain Determines the Propensity of Adult Hippocampal Neural Stem Cells to Autophagic Cell Death Following Insulin Withdrawal. Article Snippet: .. pRK5-calpain 1 and -calpain 2 plasmids were constructed by subcloning the human calpain 1 (OriGene, Rockville, MD, http://www.origene.com; SC116897) and calpain 2 (OriGene; SC119079) genes into the Cell Culture:Article Title: Autophagy regulates spermatid differentiation via degradation of PDLIM1. Article Snippet: The rat anti-LAMP2 antibody (ab13524, Abcam) was kindly provided by Dr. Li Yu (Tsinghua University). .. Plasmids , cell culture and transfection Genes encoding FLAG-tagged PDLIM1, STMN1 (stathmin 1), PPP4R2 (protein phosphatase 4, regulatory subunit 2), NUDC (nudC nuclear distribution protein), MAP6 (microtubule-associated protein 6), YBX1 (Y box protein 1), and CD2AP (CD2-associated protein) were cloned into the SalI and NotI site of the modified Transfection:Article Title: Autophagy regulates spermatid differentiation via degradation of PDLIM1. Article Snippet: The rat anti-LAMP2 antibody (ab13524, Abcam) was kindly provided by Dr. Li Yu (Tsinghua University). .. Plasmids , cell culture and transfection Genes encoding FLAG-tagged PDLIM1, STMN1 (stathmin 1), PPP4R2 (protein phosphatase 4, regulatory subunit 2), NUDC (nudC nuclear distribution protein), MAP6 (microtubule-associated protein 6), YBX1 (Y box protein 1), and CD2AP (CD2-associated protein) were cloned into the SalI and NotI site of the modified Modification:Article Title: Autophagy regulates spermatid differentiation via degradation of PDLIM1. Article Snippet: The rat anti-LAMP2 antibody (ab13524, Abcam) was kindly provided by Dr. Li Yu (Tsinghua University). .. Plasmids , cell culture and transfection Genes encoding FLAG-tagged PDLIM1, STMN1 (stathmin 1), PPP4R2 (protein phosphatase 4, regulatory subunit 2), NUDC (nudC nuclear distribution protein), MAP6 (microtubule-associated protein 6), YBX1 (Y box protein 1), and CD2AP (CD2-associated protein) were cloned into the SalI and NotI site of the modified |
