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primary human islets  (Prodo Labs)

 
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    Prodo Labs primary human islets
    Primary Human Islets, supplied by Prodo Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+human+islets/human+islets+primary/pm42120510-258-0-9
    Average 86 stars, based on 1 article reviews
    primary human islets - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    other:

    Article Title: Divergent cell-type specific hypoxia responses in human stem cell-derived and primary islets.
    Article Snippet: Primary human islets were obtained from Prodo Laboratories with consent from the donor’s relatives for research use.

    Article Title: Comparative and integrative single cell analysis reveals new insights into the transcriptional immaturity of stem cell-derived β cells.
    Article Snippet: Primary human islets were acquired as clusters and shipped from Prodo Laboratories, which required consent from the donor’s relatives for use in research.

    Transduction:

    Article Title: Paired box 6 gene delivery preserves beta cells and improves islet transplantation efficacy.
    Article Snippet: Whole islets or dispersed cells were cultured in RPMI-1640 medium (Thermo Fisher Scientific) supplemented with 10% (vol/vol) fetal bovine serum (FBS; Gibco) and 1% (vol/vol) penicillin and streptomycin (Thermo Fisher Scientific). .. Human islet culture and transduction Primary human islets from seven normal and five T2D donors (Appendix Table S2) were obtained from Prodo Laboratories, Inc. (Aliso Viejo, CA, USA). .. Human islets were cultured in PIM(S) complete media (Prodo Labs) and transduced with AAV-Ctrl or AAVPAX6 at a dose of 20,000 vg per islet cell.

    Article Title: Anti-ROR1 antibodies and uses thereof
    Article Snippet: Primary adipocytes were differentiated from human white preadipocytes (Promo Cell) (Hudecek et al., Blood 116:4532, 2010). .. 2000 islet equivalent of primary human islets, acinar cells, and media for islet culture were procured from Prodo labs. Human T cells were transduced with a ROR1 CAR lentivirus to express the ROR1-specific R12scFv CAR, as described in Hudecek et al. (Clinical Cancer Research 19:3153, 2013). ..



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    A) Schematic view of printing <t>primary</t> <t>cadaveric</t> <t>human</t> <t>islets</t> embedded in a 3% w/v alg/ 6% w/v MC bioink at 500 IEQ: 1 mL bioink. The printed primary islet construct is then cultured for 3-7 days to monitor for functionality and viability. Created using Biorender.com. B) Live Dead staining of printed primary islets 3 days post-printing. All scale bars convey 250 μm. Live cells are shown in green, dead cells are shown in red. C) Percentage of individual printed primary islet represented by viable cells, as indicated by green signal in Live/Dead staining (N=8). D) Location of dead cells present in individual printed primary islet as indicated by red signal in Live Dead staining. r/R of 0.0 indicates that the dead cell is present in the center of the islet, and an r/R of 1.0 indicates that the dead cell is present at the edge of the islet. E) Primary islets were printed and maintained in culture for 7 days. Islets are fluorescently stained for nuclei (DAPI), islet marker c-peptide (shown in green), and islet marker glucagon (shown in red). Scale bar representing 50 μm (*: p < 0.05). F) Glucose stimulated insulin secretion of printed primary islets after 7 days in culture G) Stimulation index from GSIS of second high glucose period normalized to second low glucose period (n=3).
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    human primary islets  (Prodo Labs)
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    Prodo Labs human primary islets
    A) Schematic view of printing <t>primary</t> <t>cadaveric</t> <t>human</t> <t>islets</t> embedded in a 3% w/v alg/ 6% w/v MC bioink at 500 IEQ: 1 mL bioink. The printed primary islet construct is then cultured for 3-7 days to monitor for functionality and viability. Created using Biorender.com. B) Live Dead staining of printed primary islets 3 days post-printing. All scale bars convey 250 μm. Live cells are shown in green, dead cells are shown in red. C) Percentage of individual printed primary islet represented by viable cells, as indicated by green signal in Live/Dead staining (N=8). D) Location of dead cells present in individual printed primary islet as indicated by red signal in Live Dead staining. r/R of 0.0 indicates that the dead cell is present in the center of the islet, and an r/R of 1.0 indicates that the dead cell is present at the edge of the islet. E) Primary islets were printed and maintained in culture for 7 days. Islets are fluorescently stained for nuclei (DAPI), islet marker c-peptide (shown in green), and islet marker glucagon (shown in red). Scale bar representing 50 μm (*: p < 0.05). F) Glucose stimulated insulin secretion of printed primary islets after 7 days in culture G) Stimulation index from GSIS of second high glucose period normalized to second low glucose period (n=3).
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    https://www.bioz.com/product/primary+human+islets/primary+human+islets/pm38754432-887-0-11
    Average 90 stars, based on 1 article reviews
    human primary islets - by Bioz Stars, 2026-10
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    Image Search Results


    A) Schematic view of printing primary cadaveric human islets embedded in a 3% w/v alg/ 6% w/v MC bioink at 500 IEQ: 1 mL bioink. The printed primary islet construct is then cultured for 3-7 days to monitor for functionality and viability. Created using Biorender.com. B) Live Dead staining of printed primary islets 3 days post-printing. All scale bars convey 250 μm. Live cells are shown in green, dead cells are shown in red. C) Percentage of individual printed primary islet represented by viable cells, as indicated by green signal in Live/Dead staining (N=8). D) Location of dead cells present in individual printed primary islet as indicated by red signal in Live Dead staining. r/R of 0.0 indicates that the dead cell is present in the center of the islet, and an r/R of 1.0 indicates that the dead cell is present at the edge of the islet. E) Primary islets were printed and maintained in culture for 7 days. Islets are fluorescently stained for nuclei (DAPI), islet marker c-peptide (shown in green), and islet marker glucagon (shown in red). Scale bar representing 50 μm (*: p < 0.05). F) Glucose stimulated insulin secretion of printed primary islets after 7 days in culture G) Stimulation index from GSIS of second high glucose period normalized to second low glucose period (n=3).

    Journal: bioRxiv

    Article Title: Stress-free Bioprinting of Human Primary and iPSC-derived Islets with Retained Functionality

    doi: 10.1101/2024.10.14.617656

    Figure Lengend Snippet: A) Schematic view of printing primary cadaveric human islets embedded in a 3% w/v alg/ 6% w/v MC bioink at 500 IEQ: 1 mL bioink. The printed primary islet construct is then cultured for 3-7 days to monitor for functionality and viability. Created using Biorender.com. B) Live Dead staining of printed primary islets 3 days post-printing. All scale bars convey 250 μm. Live cells are shown in green, dead cells are shown in red. C) Percentage of individual printed primary islet represented by viable cells, as indicated by green signal in Live/Dead staining (N=8). D) Location of dead cells present in individual printed primary islet as indicated by red signal in Live Dead staining. r/R of 0.0 indicates that the dead cell is present in the center of the islet, and an r/R of 1.0 indicates that the dead cell is present at the edge of the islet. E) Primary islets were printed and maintained in culture for 7 days. Islets are fluorescently stained for nuclei (DAPI), islet marker c-peptide (shown in green), and islet marker glucagon (shown in red). Scale bar representing 50 μm (*: p < 0.05). F) Glucose stimulated insulin secretion of printed primary islets after 7 days in culture G) Stimulation index from GSIS of second high glucose period normalized to second low glucose period (n=3).

    Article Snippet: [ – ] Human cadaveric primary islets (Prodo Labs) were printed in the alginate/methylcellulose ink and maintained in culture for 3 to 7 days ( ).

    Techniques: Construct, Cell Culture, Staining, Marker