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primary human islets  (BioChain Institute)

 
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    BioChain Institute primary human islets
    Primary Human Islets, supplied by BioChain Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+human+islets/primary+human+islets/pmc12181959-185-0-3
    Average 90 stars, based on 1 article reviews
    primary human islets - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Modeling diabetic alpha cell dysfunction using stem cell-derived alpha cells
    Article Snippet: Primary human islets (BioChain) or SC-α cell clusters were fixed with 4% PFA, washed three times with PBS, embedded in Histogel, and subsequently embedded in paraffin.



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    A) Schematic view of printing <t>primary</t> <t>cadaveric</t> <t>human</t> <t>islets</t> embedded in a 3% w/v alg/ 6% w/v MC bioink at 500 IEQ: 1 mL bioink. The printed primary islet construct is then cultured for 3-7 days to monitor for functionality and viability. Created using Biorender.com. B) Live Dead staining of printed primary islets 3 days post-printing. All scale bars convey 250 μm. Live cells are shown in green, dead cells are shown in red. C) Percentage of individual printed primary islet represented by viable cells, as indicated by green signal in Live/Dead staining (N=8). D) Location of dead cells present in individual printed primary islet as indicated by red signal in Live Dead staining. r/R of 0.0 indicates that the dead cell is present in the center of the islet, and an r/R of 1.0 indicates that the dead cell is present at the edge of the islet. E) Primary islets were printed and maintained in culture for 7 days. Islets are fluorescently stained for nuclei (DAPI), islet marker c-peptide (shown in green), and islet marker glucagon (shown in red). Scale bar representing 50 μm (*: p < 0.05). F) Glucose stimulated insulin secretion of printed primary islets after 7 days in culture G) Stimulation index from GSIS of second high glucose period normalized to second low glucose period (n=3).
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    human primary islets  (Prodo Labs)
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    A) Schematic view of printing <t>primary</t> <t>cadaveric</t> <t>human</t> <t>islets</t> embedded in a 3% w/v alg/ 6% w/v MC bioink at 500 IEQ: 1 mL bioink. The printed primary islet construct is then cultured for 3-7 days to monitor for functionality and viability. Created using Biorender.com. B) Live Dead staining of printed primary islets 3 days post-printing. All scale bars convey 250 μm. Live cells are shown in green, dead cells are shown in red. C) Percentage of individual printed primary islet represented by viable cells, as indicated by green signal in Live/Dead staining (N=8). D) Location of dead cells present in individual printed primary islet as indicated by red signal in Live Dead staining. r/R of 0.0 indicates that the dead cell is present in the center of the islet, and an r/R of 1.0 indicates that the dead cell is present at the edge of the islet. E) Primary islets were printed and maintained in culture for 7 days. Islets are fluorescently stained for nuclei (DAPI), islet marker c-peptide (shown in green), and islet marker glucagon (shown in red). Scale bar representing 50 μm (*: p < 0.05). F) Glucose stimulated insulin secretion of printed primary islets after 7 days in culture G) Stimulation index from GSIS of second high glucose period normalized to second low glucose period (n=3).
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    A) Schematic view of printing primary cadaveric human islets embedded in a 3% w/v alg/ 6% w/v MC bioink at 500 IEQ: 1 mL bioink. The printed primary islet construct is then cultured for 3-7 days to monitor for functionality and viability. Created using Biorender.com. B) Live Dead staining of printed primary islets 3 days post-printing. All scale bars convey 250 μm. Live cells are shown in green, dead cells are shown in red. C) Percentage of individual printed primary islet represented by viable cells, as indicated by green signal in Live/Dead staining (N=8). D) Location of dead cells present in individual printed primary islet as indicated by red signal in Live Dead staining. r/R of 0.0 indicates that the dead cell is present in the center of the islet, and an r/R of 1.0 indicates that the dead cell is present at the edge of the islet. E) Primary islets were printed and maintained in culture for 7 days. Islets are fluorescently stained for nuclei (DAPI), islet marker c-peptide (shown in green), and islet marker glucagon (shown in red). Scale bar representing 50 μm (*: p < 0.05). F) Glucose stimulated insulin secretion of printed primary islets after 7 days in culture G) Stimulation index from GSIS of second high glucose period normalized to second low glucose period (n=3).

    Journal: bioRxiv

    Article Title: Stress-free Bioprinting of Human Primary and iPSC-derived Islets with Retained Functionality

    doi: 10.1101/2024.10.14.617656

    Figure Lengend Snippet: A) Schematic view of printing primary cadaveric human islets embedded in a 3% w/v alg/ 6% w/v MC bioink at 500 IEQ: 1 mL bioink. The printed primary islet construct is then cultured for 3-7 days to monitor for functionality and viability. Created using Biorender.com. B) Live Dead staining of printed primary islets 3 days post-printing. All scale bars convey 250 μm. Live cells are shown in green, dead cells are shown in red. C) Percentage of individual printed primary islet represented by viable cells, as indicated by green signal in Live/Dead staining (N=8). D) Location of dead cells present in individual printed primary islet as indicated by red signal in Live Dead staining. r/R of 0.0 indicates that the dead cell is present in the center of the islet, and an r/R of 1.0 indicates that the dead cell is present at the edge of the islet. E) Primary islets were printed and maintained in culture for 7 days. Islets are fluorescently stained for nuclei (DAPI), islet marker c-peptide (shown in green), and islet marker glucagon (shown in red). Scale bar representing 50 μm (*: p < 0.05). F) Glucose stimulated insulin secretion of printed primary islets after 7 days in culture G) Stimulation index from GSIS of second high glucose period normalized to second low glucose period (n=3).

    Article Snippet: [ – ] Human cadaveric primary islets (Prodo Labs) were printed in the alginate/methylcellulose ink and maintained in culture for 3 to 7 days ( ).

    Techniques: Construct, Cell Culture, Staining, Marker