Journal: Metabolism: clinical and experimental

Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice

doi: 10.1016/j.metabol.2026.156548

Figure Lengend Snippet: Critical role of SRF in regulating actin cytoskeletal gene expression in adipocytes in vitro . (A) Motif identified by MEME that is enriched in H3K27ac peaks within the HFD-associated super-enhancer regions. (B) Alignment of the SRF binding motif identified de novo from SRF ChIP-Seq in TGFβ1-treated 3T3L1 adipocytes, compared to the canonical SRF motif from the HOMER database. (C) Pathway analysis of genes associated with SRF ChIP-seq peaks. (D) Genomic tracks showing SRF and H3K27ac ChIP-seq signals at the Acta2 locus, highlighting SRF binding induced by TGFβ1 treatment (indicated by a black arrow) within an adipocyte super-enhancer region. (E–G) In vitro loss- and gain-of function experiments in 3T3-L1 adipocytes. Gene expression analysis of cytoskeletal genes following (E) Srf knockdown ( shSrf , n = 3 per condition, total N = 6) and (F) overexpression ( Srf OE, n = 3 per condition, total N = 6). (G) Western blot analysis of SRF and ACTA2 protein levels upon Srf overexpression ( Srf OE, n = 2 per condition, total N = 4). A two-tailed Student’s t -test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: 3T3-L1 preadipocytes (ATCC) were maintained in DMEM (Invitrogen) supplemented with 10% bovine calf serum (HyClone) and 1% penicillin–streptomycin (Gibco).

Techniques: Gene Expression, In Vitro, Binding Assay, ChIP-sequencing, Knockdown, Over Expression, Western Blot, Two Tailed Test

Journal: Food Science & Nutrition

Article Title: A Herbal Pair of Taraxacum officinale F.H.Wigg. and Lonicera japonica Thunb. Ameliorates Obesity and Modulates AMPK Signaling

doi: 10.1002/fsn3.71774

Figure Lengend Snippet: Effect of PL‐700 on lipid accumulation in 3T3‐L1 cells. (A) Oil red O staining of fully differentiated 3T3‐L1 adipocytes treated with LIPO‐700 (1, 10, and 100 μg/mL) for 24 h on day 7 of differentiation. The histogram shows quantification of lipid content expressed as a percentage relative to the MDI‐only group. (B) Western blot analysis of p‐AMPK, AMPK, PEPCK, G6Pase, and GAPDH protein expression in 3T3‐L1 adipocytes after 24‐h treatment with LIPO‐700. (C) Western blot analysis of ATGL and HSL protein expression in 3T3‐L1 adipocytes after 24‐h treatment with LIPO‐700. GAPDH was used as a loading control. (D) RT‐PCR analysis of SREBP‐1c, leptin, and LPL mRNA expression in 3T3‐L1 adipocytes following 24‐h LIPO‐700 treatment. GAPDH was used as an internal control. ### p < 0.001, vs. untreated control; * p < 0.05, ** p < 0.01, *** p < 0.001, vs. MDI‐treated group.

Article Snippet: Mouse preadipocyte 3T3‐L1 cells (CVCL_0123; Cat# CL‐173, ATCC, Manassas, VA, USA) were cultured in Dulbecco's Modified Eagle Medium (DMEM; Gibco, Thermo Fisher Scientific, Grand Island, NY, USA) supplemented with 10% calf serum (Gibco, Thermo Fisher Scientific, Grand Island, NY, USA) and 1% penicillin–streptomycin (Gibco, Thermo Fisher Scientific, Grand Island, NY, USA) at 37°C in a humidified atmosphere containing 5% CO 2 (Jackson et al. ; Kaczmarek et al. ).

Techniques: Staining, Western Blot, Expressing, Control, Reverse Transcription Polymerase Chain Reaction