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Image Search Results
Journal: iScience
Article Title: Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation
doi: 10.1016/j.isci.2024.109672
Figure Lengend Snippet:
Article Snippet:
Techniques: Infection, Recombinant, Electron Microscopy, SYBR Green Assay, Lysis, Protease Inhibitor, Plasmid Preparation, XF Assay, Isolation, Quantitation Assay, Bicinchoninic Acid Protein Assay, Software
Journal: Data in Brief
Article Title: Updating “Data on changes in lipid profiles during the differentiation and maturation of human subcutaneous white adipocytes analyzed using chromatographic and bioinformatic tools” with data on the changes in protein profiles
doi: 10.1016/j.dib.2023.109036
Figure Lengend Snippet: Schematic of the experimental procedure. Schedule from cell culture to proteomic analysis. Human subcutaneous white preadipocytes were seeded in a 12-well plate on day 0. Twenty-four hours after seeding, spindle-shaped cells were observed. After approximately seven days, the preadipocytes achieved 100% confluence on preadipocyte growth medium (Stage-1). The preadipocytes were then differentiated for three consecutive days in preadipocyte differentiation medium (Stage-2). Three days after replacement with adipocyte nutrition medium, small lipid droplets appeared (Stage-3). The number of lipid droplets gradually increased (Stage-4) until the cytoplasm was filled with lipid droplets (Stage-5). Proteins were then extracted and cleaved with trypsin. The peptides were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS). Data were analyzed using the Proteome Discoverer 2.2 using the Mascot 2.6 search engine.
Article Snippet: The
Techniques: Cell Culture, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy
Journal: Data in Brief
Article Title: Updating “Data on changes in lipid profiles during the differentiation and maturation of human subcutaneous white adipocytes analyzed using chromatographic and bioinformatic tools” with data on the changes in protein profiles
doi: 10.1016/j.dib.2023.109036
Figure Lengend Snippet: Partial Least Squares Discriminant Analysis (PLS-DA) of proteins among the five stages. Stage-1: subcutaneous preadipocytes, stage-2: after inducing adipocyte differentiation, stages-3 to -5: from initiation of lipid droplet formation to appearance of mature subcutaneous adipocytes (dependent on lipid droplet amount and formation). The ellipses represent the 95% confidence interval. This figure was generated using MetaboAnalyst 5.0.
Article Snippet: The
Techniques: Generated