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preadipocyte nutrition medium  (PromoCell)


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    PromoCell preadipocyte nutrition medium
    Preadipocyte Nutrition Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/preadipocyte+basal+medium/Preadipocyte+Basal+Medium/pmc12347182-158-21-24
    Average 93 stars, based on 6 article reviews
    preadipocyte nutrition medium - by Bioz Stars, 2026-09
    93/100 stars

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    Cell Culture:

    Article Title: Human Adipose Tissue Macrophages Display Activation of Cancer-related Pathways
    Article Snippet: .. The CD14 − fraction (containing endothelial cells, fibroblasts, lymphocytes, stem cells, and preadipocytes) was cultured in preadipocyte basal medium (Promocell) for 24 h. Adherent preadipocytes were then cultured in selective preadipocyte growth medium (PGM, Promocell) during 3 days before collecting the preadipocyte-conditioned medium (PCM). ..

    Article Title: Bone Marrow p16 INK4a -Deficiency Does Not Modulate Obesity, Glucose Homeostasis or Atherosclerosis Development
    Article Snippet: SVF was subjected to Magnetic-Activated Cell Sorting (MACS) in order to isolate ATM CD11b + cells using CD11b-labelled magnetic beads (Miltenyi Biotec) according to the manufacturer's instructions. .. The CD11b − fraction was cultured in Preadipocyte Basal Medium (PBM, Promocell) for 24 h to eliminate cell types other than preadipocytes. ..

    Article Title: Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages
    Article Snippet: Cells were cultured depending on the experiment at a density of 1 or 2.10 6 cells/well in six-well plastic culture dishes (Primaria, Becton Dickinson Labware). .. The CD14− fraction was cultured in Preadipocyte Basal Medium (Promocell) for 24 hours, washed with PBS to remove floating cells. .. After 2 washing steps with PBS, cells were cultured in RPMI 1640 medium, containing gentamycin (40 μg/ml), glutamine (0,05%), supplemented with 10% pooled human serum (Biowest).

    Article Title: Human Adipose Tissue Macrophages Display Activation of Cancer-related Pathways
    Article Snippet: .. The CD14 fraction (containing endothelial cells, fibroblasts, lymphocytes, stem cells, and preadipocytes) was cultured in preadipocyte basal medium (Promocell) for 24 h. Adherent preadipocytes were then cultured in selective preadipocyte growthmedium (PGM, Promocell) during 3 days before collecting the preadipocyte-conditioned medium (PCM). ..

    Concentration Assay:

    Article Title: Cancer cell migration depends on adjacent ASC and adipose spheroids in a 3D bioprinted breast cancer model.
    Article Snippet: .. Alternatively, after the 2 d of spheroid assembly, adipogenic differentiation was induced by culture in adipogenic differentiation medium consisting of Preadipocyte Basal Medium-2 supplemented with 10% FCS, 1% penicillin/streptomycin, and insulin (final concentration 1.7 μM; PromoCell, Heidelberg, Germany), dexamethasone (1 μM; Sigma-Aldrich, St. Louis, USA), 3-isobutyl-1-methylxanthine (IBMX, 500 μM; ServaElectrophoresis, Heidelberg, Germany) and indomethacin (200 μM; Sigma-Aldrich, St. Louis, USA) for 9 d, with media exchange every other day. ..



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    (A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to <t>preadipocyte</t> growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.
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    Image Search Results


    (A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to preadipocyte growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.

    Journal: Frontiers in Endocrinology

    Article Title: Mouse vascularized adipose spheroids: an organotypic model for thermogenic adipocytes

    doi: 10.3389/fendo.2024.1396965

    Figure Lengend Snippet: (A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to preadipocyte growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.

    Article Snippet: The isolated inguinal white adipose tissue stromal vascular fraction was resuspended in Preadipocyte Basal Medium (BulletKit, Lonza, PT-8002) with the addition of L-glutamine, GA-1000 and FBS (SingleQuots, part of BulletKit, Lonza, PT-8002) and seeded in 12–24-wells (Thermo Scientific, 150628 and 142475) depending on the experiment.

    Techniques: Isolation, Light Microscopy, Immunofluorescence, Staining, Marker, Expressing, Standard Deviation, MANN-WHITNEY