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The expression levels of PP2Acα, p-mTOR, Acsl4, and TfR in HCT116 CRC cell lines (A) Protein electrophoresis image; (B-E) Protein quantification images of PP2Acα, p-mTOR, Acsl4, and TfR, respectively * P < 0.05, ** P < 0.01. n=3, Mean ± SD.
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The expression levels of PP2Acα, p-mTOR, Acsl4, and TfR in HCT116 CRC cell lines (A) Protein electrophoresis image; (B-E) Protein quantification images of PP2Acα, p-mTOR, Acsl4, and TfR, respectively * P < 0.05, ** P < 0.01. n=3, Mean ± SD.

Journal: Frontiers in Oncology

Article Title: PPP2CA knockdown upregulates the expression levels of ferroptosis-related genes TFRC and ACSL4 in colorectal cancer cells by promoting mTOR phosphorylation

doi: 10.3389/fonc.2025.1694932

Figure Lengend Snippet: The expression levels of PP2Acα, p-mTOR, Acsl4, and TfR in HCT116 CRC cell lines (A) Protein electrophoresis image; (B-E) Protein quantification images of PP2Acα, p-mTOR, Acsl4, and TfR, respectively * P < 0.05, ** P < 0.01. n=3, Mean ± SD.

Article Snippet: The anti-PP2AC antibody (cat. no. 13482-1-AP) and anti-GAPDH antibody (cat. no. 10494-1-AP) were acquired from Wuhan Sanying Biotechnology Co., Ltd. Additionally, the anti-mTOR (phospho Ser2448) antibody (cat. no. YP-Ab-14329), anti-ACSL4 antibody (cat. no. YP-Ab-12589), and anti-TfR antibody (cat. no. YP-Ab-14016) were purchased from Hangzhou Zhenyoupin Biotechnology Co., Ltd. Rapamycin (cat. no. R485503) was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd.

Techniques: Expressing, Protein Electrophoresis