pot1 (Novus Biologicals)

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Novus Biologicals pot1
Pot1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pot1/POT1+Antibody/pmc12993239-119-60-62
Average 94 stars, based on 21 article reviews

pot1 - by Bioz Stars, 2026-10

94/100 stars

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Western Blot:

Article Title: Deregulated DNA ADP-ribosylation impairs telomere replication
Article Snippet: Dot blot: Pan-ADP-ribose (#MABE1016, Millipore, 1:1000); Poly/Mono-ADP-ribose (#83732, Lot #5, Cell Signaling Technology, 1:1000); Mono-ADP-ribose (#HCA354 Lot #162452, Biorad, 1:1000); Auto anti-dsDNA (#AB_10805293, University of Iowa, Developmental Studies Hybridoma Bank, 1:200); Auto anti-ssDNA #AB_10805144, University of Iowa, Developmental Studies Hybridoma Bank, 1:200). .. Western blot: pRPA32 (S33) (#A300-246A, Lot #12, Bethyl, 1:1000); pRPA32 (S4/S8) (#A300-245A, Lot #9, Bethyl, 1:1000); pChk1 (S317) (#2344S, Lot #12, Cell Signaling Technology, 1:1000); Chk1 (#37010S, Lot #1, Cell Signaling Technology, 1:1000); gH2AX (#2577S Lot #12, Cell Signaling Technology, 1:1000); H2AX (#2595S Lot #8, Cell Signaling Technology, 1:1000); GFP (#130-091-833 Lot #5240106116, Miltenyi Biotech, 1:5000); PARG (#66564 Lot #1, Cell Signaling Technology, 1:1000); PARP1 (#61639 Lot #22922002, Active Motif, 1:1000); PARP2 (#39044 Lot #23822008, Active Motif, 1:1000); TARG1 (#25249-1-AP Lot #00106148, Proteintech, 1:1000); Pan-ADP-ribose (#MABE1016, Millipore, 1:1000); Poly/Mono-ADP-ribose (#83732, Lot #5, Cell Signaling Technology, 1:1000); Cyclin E2 (#4132S Lot #3, Cell Signaling Technology, 1:1000); HPF1 (#NBP1-93973 Lot #000004509, Novus, 1:1000); ARH3 (#sc-374162 Lot #G0919, Santa Cruz, 1:500); Tubulin (#T6557, Sigma-Aldrich, 1:5000); Poly-ADP-ribose (10H) (#ALX-804-220-R100, Enzo, 1:1000); FEN1 (#A300-256A, Lot #2, Fortis Life Sciences, 1:1000); DNA Ligase 1 (#A301-136A, Fortis Life Sciences, 1:1000); POT1 (#NB500-176, Novus, 1:1000); Myc (#MA1-21316, Thermo Fisher, 1:1000). .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging ary April 2023 Antibodies Antibodies used Immunofluorescence: FLAG (D6W5B) (#14793S, Cell Signaling Technology, 1:1000); FLAG (M2) (#F1804, Millipore, 1:1000); TRF1 (#ab10579, Abcam, 1:1000); TRF2 (#NB110-57130, Novus, 1:1000); RPA2 (#ab2175 Lot #GR3386072-1, Abcam, 1:1000), Poly/MonoADP-ribose (#83732, Lot #5, Cell Signaling Technology, 1:1000); Mono-ADP-ribose (#HCA354 Lot #162452, Biorad, 1:1000); MonoADP-ribose (#HCA355 Lot #158442, Biorad, 1:1000); Cas9 (Cat. #14697 Lot #8, Cell Signaling Technology, 1:1000); GFP #ab6556, Abcam, 1:1000); Alexa Fluor 594 goat anti-rabbit (#A11012 Lot #2433881, Thermo Fisher, 1:1000); Alexa Fluor 594 goat anti-mouse. (#A11032 Lot #2527968, Thermo Fisher, 1:1000); Alexa Fluor 488 goat anti-mouse (#A11001 Lot #2659299, Thermo Fisher, 1:1000); Alexa Fluor 488 goat anti-rabbit. (#A32731 Lot #XD343356, Thermo Fisher, 1:1000); Alexa Fluor 647 goat anti-mouse. (#A21244 Lot #2527970, Thermo Fisher, 1:1000).

Article Title: Deregulated DNA ADP-ribosylation impairs telomere replication.
Article Snippet: Dot blot: Pan-ADP-ribose (#MABE1016, Millipore, 1:1000); Poly/Mono-ADP-ribose (#83732, Lot #5, Cell Signaling Technology, 1:1000); Mono-ADP-ribose (#HCA354 Lot #162452, Biorad, 1:1000); Auto anti-dsDNA (#AB_10805293, University of Iowa, Developmental Studies Hybridoma Bank, 1:200); Auto anti-ssDNA #AB_10805144, University of Iowa, Developmental Studies Hybridoma Bank, 1:200). .. Western blot: pRPA32 (S33) (#A300-246A, Lot #12, Bethyl, 1:1000); pRPA32 (S4/S8) (#A300-245A, Lot #9, Bethyl, 1:1000); pChk1 (S317) (#2344S, Lot #12, Cell Signaling Technology, 1:1000); Chk1 (#37010S, Lot #1, Cell Signaling Technology, 1:1000); gH2AX (#2577S Lot #12, Cell Signaling Technology, 1:1000); H2AX (#2595S Lot #8, Cell Signaling Technology, 1:1000); GFP (#130-091-833 Lot #5240106116, Miltenyi Biotech, 1:5000); PARG (#66564 Lot #1, Cell Signaling Technology, 1:1000); PARP1 (#61639 Lot #22922002, Active Motif, 1:1000); PARP2 (#39044 Lot #23822008, Active Motif, 1:1000); TARG1 (#25249-1-AP Lot #00106148, Proteintech, 1:1000); Pan-ADP-ribose (#MABE1016, Millipore, 1:1000); Poly/Mono-ADP-ribose (#83732, Lot #5, Cell Signaling Technology, 1:1000); Cyclin E2 (#4132S Lot #3, Cell Signaling Technology, 1:1000); HPF1 (#NBP1-93973 Lot #000004509, Novus, 1:1000); ARH3 (#sc-374162 Lot #G0919, Santa Cruz, 1:500); Tubulin (#T6557, Sigma-Aldrich, 1:5000); Poly-ADP-ribose (10H) (#ALX-804-220-R100, Enzo, 1:1000); FEN1 (#A300-256A, Lot #2, Fortis Life Sciences, 1:1000); DNA Ligase 1 (#A301-136A, Fortis Life Sciences, 1:1000); POT1 (#NB500-176, Novus, 1:1000); Myc (#MA1-21316, Thermo Fisher, 1:1000). ..

Pyrolysis Gas Chromatography:

Article Title: Modified hTERT treatment ameliorates pressure overload-induced heart failure.
Article Snippet: .. The primary antibodies: histone H 2 AX (1:1000, Cell Signalling Technology, 7631S), γH 2 AX (1:1000, Cell Signalling Technology, 9718S), 53BP1(1:1000, Cell Signalling Technology, 4937S), p53 (1:1000, Cell Signalling Technology, 2524S), Phospho-p53 (Ser15) (1:1000, Cell Signalling Technology, 9284s), PGC-1α (1:1000, Santa Cruz, sc-517380), TFAM (1:1000, Proteintech, 22586-1-AP), TRF1 (1:1000, Abcam, ab192629), RAP1 (1:1000, Santa Cruz, sc-53434), TIN2 (1:1000, Proteintech, 11368-1-AP), POT1 (1:1000, Novus Biologicals, NB500-176), HA-tag (1:1000, Cell Signalling Technology, 3724s), DYKDDDDK Tag (1:1000, Cell Signalling Technology, 14793s), H3 (1:1000, Cell Signalling Technology, D5A7), GAPDH (1:1000, Proteintech, 60004-1-Ig) followed by secondary antibody for 1 h with a 1:2000 dilution of IgG Goat Anti-Mouse HRP (Proteintech, SA00001-1) or IgG Goat Anti- Rabbit HRP (Proteintech, SA00001-2). .. Western blots were developed using ECL chemiluminescent substrate (Thermo Fisher Scientific, 34098CN).

Article Title: Modified hTERT treatment ameliorates pressure overload-induced heart failure
Article Snippet: .. The primary antibodies: histone H 2 AX (1:1000, Cell Signalling Technology, 7631S), γH 2 AX (1:1000, Cell Signalling Technology, 9718S), 53BP1(1:1000, Cell Signalling Technology, 4937S), p53 (1:1000, Cell Signalling Technology, 2524S), Phospho-p53 (Ser15) (1:1000, Cell Signalling Technology, 9284s), PGC-1α (1:1000, Santa Cruz, sc-517380), TFAM (1:1000, Proteintech, 22586-1-AP), TRF1 (1:1000, Abcam, ab192629), RAP1 (1:1000, Santa Cruz, sc-53434), TIN2 (1:1000, Proteintech, 11368-1-AP), POT1 (1:1000, Novus Biologicals, NB500-176), HA-tag (1:1000, Cell Signalling Technology, 3724s), DYKDDDDK Tag (1:1000, Cell Signalling Technology, 14793s), H3 (1:1000, Cell Signalling Technology, D5A7), GAPDH (1:1000, Proteintech, 60004-1-Ig) followed by secondary antibody for 1 h with a 1:2000 dilution of IgG Goat Anti-Mouse HRP (Proteintech, SA00001-1) or IgG Goat Anti- Rabbit HRP (Proteintech, SA00001-2). .. Western blots were developed using ECL chemiluminescent substrate (Thermo Fisher Scientific, 34098CN).

Membrane:

Article Title: BRM-SWI/SNF chromatin remodeling complex enables functional telomeres by promoting co-expression of TRF2 and TRF1
Article Snippet: .. Proteins were separated by SDS–PAGE, transferred to PVDF membrane, and probed with antibodies specific for p-ATM (p-S1981, 1:5000 dilution, ab81292, Abcam), ATM (1:5000 dilution, ab32420, Abcam), TRF2 (1:2000 dilution, 05–513, Merck), TRF1 (1:1000 dilution, GTX77605, Genetex), POT1 (1:2000 dilution, NB500-176, Novus Biologicals), RAP1 (1:1000 dilution, 5433, Cell Signaling Technology), TPP1 (1:1000 dilution, 14667, Cell Signaling Technology), BRM (1:2000 dilution, ab15597, Abcam), BRG1 (1:2000 dilution, 21634-1-AP, Proteintech) and Flag (1:5000 dilution, F1804, Sigma). β-actin (1:5000 dilution, 66009-1-Ig, Proteintech) antibody was used as a loading control. ..

Control:

other:

Article Title: Cyclin E-induced replicative stress drives p53-dependent whole-genome duplication.
Article Snippet: The following antibodies were used for FACS and diluted in PBS supplemented with 1% BSA: MCM7 (1:200, Santa Cruz, sc-56324), cyclin B1 (1:200, Abcam, ab32053), p-H2A.X S139 (1:200, Millipore, 05-636), anti-Rabbit Alexa Fluor 555 (1:500, Thermo, A21428) and anti-Mouse Alexa Fluor 555 (1:500, Thermo, A21424).

Blocking Assay:

Article Title: Fluorogenic speed-optimized DNA-PAINT probes enable super-resolution imaging of whole cells
Article Snippet: .. Cells were then blocked for 1 hour with blocking buffer (1% BSA in 0.1% Triton X-100), blocking buffer was then removed, and samples were labeled at 1:300 with c-myc monoclonal antibody (ThermoFisher Scientific; 9E10 cMyc) against POT1 and TRF2 (Novus Biologicals; 57130) at 4 oC overnight. .. The next day, samples were washed 3 times with 1× PBS for 5 minutes each and incubated in DNA-labeled secondary nanobodies (Massive Photonics) corresponding to their host target for 1 h in the blocking buffer .

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pot1 (Novus Biologicals)

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