polyacrylamide gel substrates (Bio-Rad)

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Bio-Rad polyacrylamide gel substrates

Schematic illustration of experimental process to find novel genes involved in biophysical stimulation. (A) The four sample conditions used in this study: shCTRL and shDDR2 cells cultured on 2 and 20 kPa collagen‐coated <t>polyacrylamide</t> gel <t>substrates.</t> (B) Experimental process of RNA‐seq: a 24‐h incubation of the cells on PAA gels followed by cellular RNA extraction, library construction, sequencing, and data analysis. This figure is created with BioRender.com .

Polyacrylamide Gel Substrates, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 10229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyacrylamide+gel+substrates/Acrylamide/pmc11073507-125-0-10
Average 97 stars, based on 10229 article reviews

polyacrylamide gel substrates - by Bioz Stars, 2026-11

97/100 stars

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1) Product Images from "DDR2 signaling and mechanosensing orchestrate neuroblastoma cell fate through different transcriptome mechanisms"

Article Title: DDR2 signaling and mechanosensing orchestrate neuroblastoma cell fate through different transcriptome mechanisms

Journal: FEBS Open Bio

doi: 10.1002/2211-5463.13798

Figure Legend Snippet: Schematic illustration of experimental process to find novel genes involved in biophysical stimulation. (A) The four sample conditions used in this study: shCTRL and shDDR2 cells cultured on 2 and 20 kPa collagen‐coated polyacrylamide gel substrates. (B) Experimental process of RNA‐seq: a 24‐h incubation of the cells on PAA gels followed by cellular RNA extraction, library construction, sequencing, and data analysis. This figure is created with BioRender.com .

Techniques Used: Cell Culture, RNA Sequencing, Incubation, RNA Extraction, Sequencing

Oligomer Restriction:

Article Title: JunB Mediates Basal- and TGFβ1-Induced Smooth Muscle Cell Contractility
Article Snippet: .. Briefly, polyacrylamide gel substrates were prepared by mixing acrylamide, bis-acrylamide (Bio-Rad, Hercules, CA), and 0.5 μm diameter yellow fluorescent beads (Invitrogen, Eugene, OR) in ultrapure water. .. The mixture was then added to the center of pretreated 20 mm diameter glass-bottomed wells of 6-well plates (In Vitro Scientific, Sunnyvale, CA).

other:

Article Title: Mechanosensitive traction force generation is regulated by the neutrophil activation state.
Article Snippet: Polyacrylamide gel substrates were then prepared using varying concentrations of acrylamide (Bio-Rad) and N,N-methylene-bisacrylamide (Bio-Rad) to achieve either a Young’s modulus of either 1.5 kPa or 10 kPa; 1.5 kPa substrates were made with 3% acrylamide and 0.2% bisacrylamide, and 10 kPa substrates with 5.2% acrylamide and 0.19% bisacrylamide.

Article Title: Mechanosensitive Traction Force Generation is Regulated by the Neutrophil Activation State
Article Snippet: Polyacrylamide gel substrates were then prepared using varying concentrations of acrylamide (Bio-Rad) and N,N-methylene-bisacrylamide (Bio-Rad) to achieve either a Young’s modulus of either 1.5 kPa or 10 kPa; 1.5 kPa substrates were made with 3% acrylamide and 0.2% bisacrylamide, and 10 kPa substrates with 5.2% acrylamide and 0.19% bisacrylamide.

Article Title: Mechanosensitive traction force generation is regulated by the neutrophil activation state
Article Snippet: The bottom coverslip was functionalized with 0.5% 3-Aminopropyltriethoxysilane (Sigma 281778) in ethanol followed by 0.5% glutaraldehyde (Polyscience 07710-100) in deionized water for hydrophilicity.

Concentration Assay:

Article Title: ECM stiffness primes the TGFβ pathway to promote chondrocyte differentiation
Article Snippet: .. The elastic modulus of polyacrylamide gel substrates was controlled by varying the concentration of the cross-linker, piperazine diacrylamide (Bio-Rad, Hercules, CA 161-0202), from 1–3% (wt/vol), while maintaining a constant 30% (wt/vol) concentration of the monomer, acrylamide (161, 0140; Bio-Rad), in a solution of 0.01 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer. .. Polymerization was initiated by 10% (wt/vol) ammonium persulfate (APS) added at a 1:200 dilution and enhanced by TEMED (161-0801; Bio-Rad) added at a 1:2000 dilution.

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