poly prep gravity flow column (Bio-Rad)
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Poly Prep Gravity Flow Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1781 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+prep+gravity+flow+columns/Columns+For+Chromatography+Poly-Prep/pmc13018514-96-19-22
Average 99 stars, based on 1781 article reviews
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Purification:Article Title: Streptococcus pneumoniae DNA Initiates Type I Interferon Signaling in the Respiratory Tract Article Snippet: Cells were lysed using Bugbuster (Novagen) and sonication in the presence of the HALT protease cocktail (Pierce). .. His-tagged protein was purified from clarified lysates with nickel-charged agarose resin using Article Title: Streptococcus pneumoniae DNA Initiates Type I Interferon Signaling in the Respiratory Tract Article Snippet: Cells were lysed using Bugbuster (Novagen) and sonication in the presence of the HALT protease cocktail (Pierce). .. His-tagged protein was purified from clarified lysates with nickel-charged agarose resin using Disruption:Article Title: Crystallization and preliminary X-ray diffraction analyses of the homodimeric glycine decarboxylase (P-protein) from the cyanobacterium Synechocystis sp. PCC 6803 Article Snippet: .. After cell disruption by sonication, the lysate was centrifuged (Sorvall RC6, 20 000 rev min −1 , SS34 rotor) for 40 min at 277 K. Metal-affinity chromatography was carried out using Ni 2+ -Sepharose (GE Healthcare, Sweden) and Sonication:Article Title: Crystallization and preliminary X-ray diffraction analyses of the homodimeric glycine decarboxylase (P-protein) from the cyanobacterium Synechocystis sp. PCC 6803 Article Snippet: .. After cell disruption by sonication, the lysate was centrifuged (Sorvall RC6, 20 000 rev min −1 , SS34 rotor) for 40 min at 277 K. Metal-affinity chromatography was carried out using Ni 2+ -Sepharose (GE Healthcare, Sweden) and Chromatography:Article Title: Crystallization and preliminary X-ray diffraction analyses of the homodimeric glycine decarboxylase (P-protein) from the cyanobacterium Synechocystis sp. PCC 6803 Article Snippet: .. After cell disruption by sonication, the lysate was centrifuged (Sorvall RC6, 20 000 rev min −1 , SS34 rotor) for 40 min at 277 K. Metal-affinity chromatography was carried out using Ni 2+ -Sepharose (GE Healthcare, Sweden) and Incubation:Article Title: An intrinsic mechanism of secreted protein aging and turnover Article Snippet: Plasma protein samples were diluted 1:10 in Tris-buffered saline (TBS) and incubated with lectin-agarose affinity matrices of Erythrina cristagalli (ECA), Ricinus communis agglutinin-I (RCA), peanut agglutinin (PNA), Maackia amurensis -II (MAL-II), Sambucus nigra (SNA), wheat germ agglutinin (WGA), Griffonia simplicifolia -II (GSL-II), Con A, or Lens culinaris (LCA) (Vector Laboratories). .. After incubation overnight at 4 °C on a rotating wheel, the mixture was applied to Article Title: Innate mechanism of mucosal barrier erosion in the pathogenesis of acquired colitis Article Snippet: .. After centrifugation at 14,000 rpm, the supernatants was incubated with 2 μg/ml of Muc2 antibody (R-12, Santa Cruz Biotechnology) and protein A/G PLUS agarose for 2 h at room temperature, centrifuged at 2,000 rpm to remove agarose beads, and then applied to Centrifugation:Article Title: Innate mechanism of mucosal barrier erosion in the pathogenesis of acquired colitis Article Snippet: .. After centrifugation at 14,000 rpm, the supernatants was incubated with 2 μg/ml of Muc2 antibody (R-12, Santa Cruz Biotechnology) and protein A/G PLUS agarose for 2 h at room temperature, centrifuged at 2,000 rpm to remove agarose beads, and then applied to |
