poly prep gravity flow column (Bio-Rad)

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Bio-Rad poly prep gravity flow column
Poly Prep Gravity Flow Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1781 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+prep+gravity+flow+column/Columns+For+Chromatography+Poly-Prep/pmc13018514-96-19-22
Average 99 stars, based on 1781 article reviews

poly prep gravity flow column - by Bioz Stars, 2026-09

99/100 stars

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Chromatography:

Article Title: Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacterium Streptococcus sanguinis
Article Snippet: The clarified lysate was then mixed with 2 ml of nickel-nitrilotriacetic acid-agarose resin (Qiagen), pre-washed in binding buffer A, and incubated for 2 h at 4 °C with gentle agitation. .. This chromatography mixture was then filtered through a Poly-Prep gravity-flow column (Bio-Rad) and washed several times with binding buffer A, before the protein was eluted with elution buffer A (50 m m HEPES, pH 7.4, 200 m m NaCl, 500 m m imidazole, 1× SIGMAFAST EDTA-free protease inhibitor mixture). .. The affinity-purified proteins were further purified, and simultaneously buffer-exchanged into 50 m m HEPES, pH 7.4, 200 m m NaCl, by gel-filtration chromatography on an Akta Purifier using a Superdex 75 10/300 GL column (GE Healthcare).

Article Title: Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacterium Streptococcus sanguinis
Article Snippet: The clarified lysate was then mixed with 2 ml of Ni-NTA agarose resin (Qiagen), prewashed in binding buffer A, and incubated for 2 h at 4oC with gentle agitation. .. This chromatography mixture was then filtered through a Poly-Prep gravity-flow column (BioRad) and washed several times with by gest on M arch 9, 2019 hp://w w w .jb.org/ D ow nladed from Gram-positive type IV pili 12 binding buffer A, before the protein was eluted with elution buffer A (50 mM HEPES pH 7.4, 200 mM NaCl, 500 mM imidazole, 1 x SIGMAFAST EDTA-free protease inhibitor cocktail). .. The affinitypurified proteins were further purified, and simultaneously buffer-exchanged into (50 mM HEPES pH 7.4, 200 mM NaCl), by gel-filtration chromatography on an Akta Purifier using a Superdex 75 10/300 GL column (GE Healthcare).

Article Title: Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacterium Streptococcus sanguinis
Article Snippet: The clarified lysate was then mixed with 2 ml of Ni-NTA agarose resin (Qiagen), prewashed in binding buffer A, and incubated for 2 h at 4°C with gentle agitation. .. This chromatography mixture was then filtered through a Poly-Prep gravity-flow column (BioRad) and washed several times with binding buffer A, before the protein was eluted with elution buffer A (50 mM HEPES pH 7.4, 200 mM NaCl, 500 mM imidazole, 1 x SIGMAFAST EDTA-free protease inhibitor cocktail). .. The affinity-purified proteins were further purified, and simultaneously buffer-exchanged into (50 mM HEPES pH 7.4, 200 mM NaCl), by gel-filtration chromatography on an Akta Purifier using a Superdex 75 10/300 GL column (GE Healthcare).

Article Title: Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacterium Streptococcus sanguinis
Article Snippet: The clarified lysate was then mixed with 2 ml of Ni-NTA agarose resin (Qiagen), prewashed in binding buffer A, and incubated for 2 h at 4oC with gentle agitation. .. This chromatography mixture was then filtered through a Poly-Prep gravity-flow column (BioRad) and washed several times with by guest on M arch 9, 2019 http://w w w .jbc.org/ D ow nloaded from binding buffer A, before the protein was eluted with elution buffer A (50 mM HEPES pH 7.4, 200 mM NaCl, 500 mM imidazole, 1 x SIGMAFAST EDTA-free protease inhibitor cocktail). .. The affinitypurified proteins were further purified, and simultaneously buffer-exchanged into (50 mM HEPES pH 7.4, 200 mM NaCl), by gel-filtration chromatography on an Akta Purifier using a Superdex 75 10/300 GL column (GE Healthcare).

Binding Assay:

Article Title: Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacterium Streptococcus sanguinis
Article Snippet: The clarified lysate was then mixed with 2 ml of Ni-NTA agarose resin (Qiagen), prewashed in binding buffer A, and incubated for 2 h at 4oC with gentle agitation. .. This chromatography mixture was then filtered through a Poly-Prep gravity-flow column (BioRad) and washed several times with by gest on M arch 9, 2019 hp://w w w .jb.org/ D ow nladed from Gram-positive type IV pili 12 binding buffer A, before the protein was eluted with elution buffer A (50 mM HEPES pH 7.4, 200 mM NaCl, 500 mM imidazole, 1 x SIGMAFAST EDTA-free protease inhibitor cocktail). .. The affinitypurified proteins were further purified, and simultaneously buffer-exchanged into (50 mM HEPES pH 7.4, 200 mM NaCl), by gel-filtration chromatography on an Akta Purifier using a Superdex 75 10/300 GL column (GE Healthcare).

Article Title: Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacterium Streptococcus sanguinis
Article Snippet: The clarified lysate was then mixed with 2 ml of Ni-NTA agarose resin (Qiagen), prewashed in binding buffer A, and incubated for 2 h at 4°C with gentle agitation. .. This chromatography mixture was then filtered through a Poly-Prep gravity-flow column (BioRad) and washed several times with binding buffer A, before the protein was eluted with elution buffer A (50 mM HEPES pH 7.4, 200 mM NaCl, 500 mM imidazole, 1 x SIGMAFAST EDTA-free protease inhibitor cocktail). .. The affinity-purified proteins were further purified, and simultaneously buffer-exchanged into (50 mM HEPES pH 7.4, 200 mM NaCl), by gel-filtration chromatography on an Akta Purifier using a Superdex 75 10/300 GL column (GE Healthcare).

Article Title: Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacterium Streptococcus sanguinis
Article Snippet: The clarified lysate was then mixed with 2 ml of Ni-NTA agarose resin (Qiagen), prewashed in binding buffer A, and incubated for 2 h at 4oC with gentle agitation. .. This chromatography mixture was then filtered through a Poly-Prep gravity-flow column (BioRad) and washed several times with by guest on M arch 9, 2019 http://w w w .jbc.org/ D ow nloaded from binding buffer A, before the protein was eluted with elution buffer A (50 mM HEPES pH 7.4, 200 mM NaCl, 500 mM imidazole, 1 x SIGMAFAST EDTA-free protease inhibitor cocktail). .. The affinitypurified proteins were further purified, and simultaneously buffer-exchanged into (50 mM HEPES pH 7.4, 200 mM NaCl), by gel-filtration chromatography on an Akta Purifier using a Superdex 75 10/300 GL column (GE Healthcare).

Protease Inhibitor:

Article Title: Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacterium Streptococcus sanguinis
Article Snippet: The clarified lysate was then mixed with 2 ml of Ni-NTA agarose resin (Qiagen), prewashed in binding buffer A, and incubated for 2 h at 4oC with gentle agitation. .. This chromatography mixture was then filtered through a Poly-Prep gravity-flow column (BioRad) and washed several times with by gest on M arch 9, 2019 hp://w w w .jb.org/ D ow nladed from Gram-positive type IV pili 12 binding buffer A, before the protein was eluted with elution buffer A (50 mM HEPES pH 7.4, 200 mM NaCl, 500 mM imidazole, 1 x SIGMAFAST EDTA-free protease inhibitor cocktail). .. The affinitypurified proteins were further purified, and simultaneously buffer-exchanged into (50 mM HEPES pH 7.4, 200 mM NaCl), by gel-filtration chromatography on an Akta Purifier using a Superdex 75 10/300 GL column (GE Healthcare).

Article Title: Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacterium Streptococcus sanguinis
Article Snippet: The clarified lysate was then mixed with 2 ml of Ni-NTA agarose resin (Qiagen), prewashed in binding buffer A, and incubated for 2 h at 4°C with gentle agitation. .. This chromatography mixture was then filtered through a Poly-Prep gravity-flow column (BioRad) and washed several times with binding buffer A, before the protein was eluted with elution buffer A (50 mM HEPES pH 7.4, 200 mM NaCl, 500 mM imidazole, 1 x SIGMAFAST EDTA-free protease inhibitor cocktail). .. The affinity-purified proteins were further purified, and simultaneously buffer-exchanged into (50 mM HEPES pH 7.4, 200 mM NaCl), by gel-filtration chromatography on an Akta Purifier using a Superdex 75 10/300 GL column (GE Healthcare).

Article Title: Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacterium Streptococcus sanguinis
Article Snippet: The clarified lysate was then mixed with 2 ml of Ni-NTA agarose resin (Qiagen), prewashed in binding buffer A, and incubated for 2 h at 4oC with gentle agitation. .. This chromatography mixture was then filtered through a Poly-Prep gravity-flow column (BioRad) and washed several times with by guest on M arch 9, 2019 http://w w w .jbc.org/ D ow nloaded from binding buffer A, before the protein was eluted with elution buffer A (50 mM HEPES pH 7.4, 200 mM NaCl, 500 mM imidazole, 1 x SIGMAFAST EDTA-free protease inhibitor cocktail). .. The affinitypurified proteins were further purified, and simultaneously buffer-exchanged into (50 mM HEPES pH 7.4, 200 mM NaCl), by gel-filtration chromatography on an Akta Purifier using a Superdex 75 10/300 GL column (GE Healthcare).

Lysis:

Article Title: Structures and kinetics of Thermotoga maritima MetY reveal new insights into the predominant sulfurylation enzyme of bacterial methionine biosynthesis
Article Snippet: .. The resin was transferred to a Poly-Prep gravity flow column (Bio-Rad), and protein was eluted with the lysis buffer containing 150-mM imidazole. ..

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