Review



aavs1 locus  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Addgene inc aavs1 locus
    Aavs1 Locus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmk243/pMK243+(Tet-OsTIR1-PURO)+(Plasmid+%2372835)/bio_rxiv__64898__2026__03__20__713105-165-22-25
    Average 93 stars, based on 46 article reviews
    aavs1 locus - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis
    Article Snippet: .. To generate tetracycline-inducible PEX5 C11A plasmid, we modified pMK243 (Tet-OsTIR1-PURO) from Masato Kanemaki (Addgene #72835). pMK243 was digested by BgIII and MluI to remove the OsTIR sequence. ..

    Article Title: Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis.
    Article Snippet: .. 3, No. 10 D ow nloaded from https://academ ic.oup.com /pnasnexus/article/3/10/pgae429/7793200 by guest on 25 N ovem ber 2024 tetracycline-inducible PEX5C11A plasmid, we modified pMK243 (Tet-OsTIR1-PURO) from Masato Kanemaki (Addgene #72835). pMK243 was digested by BgIII and MluI to remove the OsTIR sequence. ..

    Article Title: A Reporter Platform to Study Therapy-Induced Senescence in Live Cancer Cells.
    Article Snippet: Dronpa-LaminB1-10 was a gift from Michael Davidson, used for the creation of the SRbr, SRbfr, SRsh, SRf and SRs constructs (Addgene plasmid #57 282); mCherry-LaminA-C-18 was a gift from Michael Davidson (Addgene plasmid #55 068); pHRM-NLS-dCas9-GFP11×7NLS-P2A-BFP-dWPRE was a gift from Bo Huang for the creation of the SRs construct (Addgene plasmid #70 224)[59]; piRFP670-N1 was a gift from Vladislav Verkhusha to create the SRbfr construct (Addgene plasmid #45 457) and pmScarlet-i_C1 was a gift from Dorus Gadella for the SRbr, SRsh, SRf and SRs constructs (Addgene plasmid #85 044). .. [60] pMK243 was a gift from Masato Kanemaki (Addgene plasmid #72 835)[61] to create the SRsh construct. .. The plasmid with mCherry expressed under a human IL6-promotor was obtained from Tebu-bio.

    Article Title: A Reporter Platform to Study Therapy‐Induced Senescence in Live Cancer Cells
    Article Snippet: Dronpa‐LaminB1‐10 was a gift from Michael Davidson, used for the creation of the SRbr, SRbfr, SRsh, SRf and SRs constructs (Addgene plasmid #57 282); mCherry‐LaminA‐C‐18 was a gift from Michael Davidson (Addgene plasmid #55 068); pHRM‐NLS‐dCas9‐GFP11×7‐NLS‐P2A‐BFP‐dWPRE was a gift from Bo Huang for the creation of the SRs construct (Addgene plasmid #70 224) [ ] ; piRFP670‐N1 was a gift from Vladislav Verkhusha to create the SRbfr construct (Addgene plasmid #45 457) and pmScarlet‐i_C1 was a gift from Dorus Gadella for the SRbr, SRsh, SRf and SRs constructs (Addgene plasmid #85 044). .. [ ] pMK243 was a gift from Masato Kanemaki (Addgene plasmid #72 835) [ ] to create the SRsh construct. .. The plasmid with mCherry expressed under a human IL6‐promotor was obtained from Tebu‐bio.

    Modification:

    Article Title: Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis
    Article Snippet: .. To generate tetracycline-inducible PEX5 C11A plasmid, we modified pMK243 (Tet-OsTIR1-PURO) from Masato Kanemaki (Addgene #72835). pMK243 was digested by BgIII and MluI to remove the OsTIR sequence. ..

    Article Title: Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis.
    Article Snippet: .. 3, No. 10 D ow nloaded from https://academ ic.oup.com /pnasnexus/article/3/10/pgae429/7793200 by guest on 25 N ovem ber 2024 tetracycline-inducible PEX5C11A plasmid, we modified pMK243 (Tet-OsTIR1-PURO) from Masato Kanemaki (Addgene #72835). pMK243 was digested by BgIII and MluI to remove the OsTIR sequence. ..

    Sequencing:

    Article Title: Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis
    Article Snippet: .. To generate tetracycline-inducible PEX5 C11A plasmid, we modified pMK243 (Tet-OsTIR1-PURO) from Masato Kanemaki (Addgene #72835). pMK243 was digested by BgIII and MluI to remove the OsTIR sequence. ..

    Article Title: Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis.
    Article Snippet: .. 3, No. 10 D ow nloaded from https://academ ic.oup.com /pnasnexus/article/3/10/pgae429/7793200 by guest on 25 N ovem ber 2024 tetracycline-inducible PEX5C11A plasmid, we modified pMK243 (Tet-OsTIR1-PURO) from Masato Kanemaki (Addgene #72835). pMK243 was digested by BgIII and MluI to remove the OsTIR sequence. ..

    Transfection:

    Article Title: RIF1 suppresses the formation of single-stranded ultrafine anaphase bridges via protein phosphatase 1.
    Article Snippet: The pEGFP-PP1a (#44224), pEGFP-PP1b (#44223) and pEGFPPP1g (#44225) were obtained from Addgene. .. Transfection and cell line generation HCT116 OsTIR1 cell line was generated by transfecting HCT116 cells with plasmids AAVS1 T2 CRIPR in pX330 (Addgene #72833) and pMK243 (Tet-OsTIR1-PURO) (Addgene #72835), as described in the previous study.44 Plasmids were transfected using Lipofectamine 2000 (Thermo Fisher) followed by puromycin selection and single clone isolation. ..

    Generated:

    Article Title: RIF1 suppresses the formation of single-stranded ultrafine anaphase bridges via protein phosphatase 1.
    Article Snippet: The pEGFP-PP1a (#44224), pEGFP-PP1b (#44223) and pEGFPPP1g (#44225) were obtained from Addgene. .. Transfection and cell line generation HCT116 OsTIR1 cell line was generated by transfecting HCT116 cells with plasmids AAVS1 T2 CRIPR in pX330 (Addgene #72833) and pMK243 (Tet-OsTIR1-PURO) (Addgene #72835), as described in the previous study.44 Plasmids were transfected using Lipofectamine 2000 (Thermo Fisher) followed by puromycin selection and single clone isolation. ..

    Selection:

    Article Title: RIF1 suppresses the formation of single-stranded ultrafine anaphase bridges via protein phosphatase 1.
    Article Snippet: The pEGFP-PP1a (#44224), pEGFP-PP1b (#44223) and pEGFPPP1g (#44225) were obtained from Addgene. .. Transfection and cell line generation HCT116 OsTIR1 cell line was generated by transfecting HCT116 cells with plasmids AAVS1 T2 CRIPR in pX330 (Addgene #72833) and pMK243 (Tet-OsTIR1-PURO) (Addgene #72835), as described in the previous study.44 Plasmids were transfected using Lipofectamine 2000 (Thermo Fisher) followed by puromycin selection and single clone isolation. ..

    Isolation:

    Article Title: RIF1 suppresses the formation of single-stranded ultrafine anaphase bridges via protein phosphatase 1.
    Article Snippet: The pEGFP-PP1a (#44224), pEGFP-PP1b (#44223) and pEGFPPP1g (#44225) were obtained from Addgene. .. Transfection and cell line generation HCT116 OsTIR1 cell line was generated by transfecting HCT116 cells with plasmids AAVS1 T2 CRIPR in pX330 (Addgene #72833) and pMK243 (Tet-OsTIR1-PURO) (Addgene #72835), as described in the previous study.44 Plasmids were transfected using Lipofectamine 2000 (Thermo Fisher) followed by puromycin selection and single clone isolation. ..

    Clone Assay:

    Article Title: NuMA promotes constitutive heterochromatin compaction by stabilizing linker histone H1 on chromatin
    Article Snippet: .. Plasmids AAVS1 T2 CRIPR in pX330 (Addgene, 72833) and pMK243 (Tet-OsTIR1-PURO) (Addgene, 72835) were co-transfected and positive clones were screened by puromycin. ..

    Construct:

    Article Title: A Reporter Platform to Study Therapy-Induced Senescence in Live Cancer Cells.
    Article Snippet: Dronpa-LaminB1-10 was a gift from Michael Davidson, used for the creation of the SRbr, SRbfr, SRsh, SRf and SRs constructs (Addgene plasmid #57 282); mCherry-LaminA-C-18 was a gift from Michael Davidson (Addgene plasmid #55 068); pHRM-NLS-dCas9-GFP11×7NLS-P2A-BFP-dWPRE was a gift from Bo Huang for the creation of the SRs construct (Addgene plasmid #70 224)[59]; piRFP670-N1 was a gift from Vladislav Verkhusha to create the SRbfr construct (Addgene plasmid #45 457) and pmScarlet-i_C1 was a gift from Dorus Gadella for the SRbr, SRsh, SRf and SRs constructs (Addgene plasmid #85 044). .. [60] pMK243 was a gift from Masato Kanemaki (Addgene plasmid #72 835)[61] to create the SRsh construct. .. The plasmid with mCherry expressed under a human IL6-promotor was obtained from Tebu-bio.

    Article Title: A Reporter Platform to Study Therapy‐Induced Senescence in Live Cancer Cells
    Article Snippet: Dronpa‐LaminB1‐10 was a gift from Michael Davidson, used for the creation of the SRbr, SRbfr, SRsh, SRf and SRs constructs (Addgene plasmid #57 282); mCherry‐LaminA‐C‐18 was a gift from Michael Davidson (Addgene plasmid #55 068); pHRM‐NLS‐dCas9‐GFP11×7‐NLS‐P2A‐BFP‐dWPRE was a gift from Bo Huang for the creation of the SRs construct (Addgene plasmid #70 224) [ ] ; piRFP670‐N1 was a gift from Vladislav Verkhusha to create the SRbfr construct (Addgene plasmid #45 457) and pmScarlet‐i_C1 was a gift from Dorus Gadella for the SRbr, SRsh, SRf and SRs constructs (Addgene plasmid #85 044). .. [ ] pMK243 was a gift from Masato Kanemaki (Addgene plasmid #72 835) [ ] to create the SRsh construct. .. The plasmid with mCherry expressed under a human IL6‐promotor was obtained from Tebu‐bio.

    other:

    Article Title: Ubiquitous MEIS transcription factors actuate lineage-specific transcription to establish cell fate
    Article Snippet: pMK243 (Tet-OsTIR1-PURO) , Addgene , 72835.



    Similar Products

    93
    Addgene inc aavs1 locus
    Aavs1 Locus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmk243/pMK243+(Tet-OsTIR1-PURO)+(Plasmid+%2372835)/bio_rxiv__64898__2026__03__20__713105-165-22-25
    Average 93 stars, based on 1 article reviews
    aavs1 locus - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Addgene inc pmk243 tet ostir1 puro
    Pmk243 Tet Ostir1 Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmk243/pMK243+(Tet-OsTIR1-PURO)+(Plasmid+%2372835)/bio_rxiv__64898__2025__12__17__694947-33-5-13
    Average 93 stars, based on 1 article reviews
    pmk243 tet ostir1 puro - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Addgene inc pmk243
    Pmk243, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmk243/pMK243+(Tet-OsTIR1-PURO)+(Plasmid+%2372835)/pm41159617-246-1-8
    Average 93 stars, based on 1 article reviews
    pmk243 - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Addgene inc tet ostir1 puro cassette
    Tet Ostir1 Puro Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmk243/pMK243+(Tet-OsTIR1-PURO)+(Plasmid+%2372835)/pm40106440-289-3-6
    Average 93 stars, based on 1 article reviews
    tet ostir1 puro cassette - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Addgene inc tet on promoter
    Tet On Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmk243/pMK243+(Tet-OsTIR1-PURO)+(Plasmid+%2372835)/pmc12000411-531-14-16
    Average 93 stars, based on 1 article reviews
    tet on promoter - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    Image Search Results